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Effects of combined pre- and post-natal protein deprivation on the myenteric plexus of the esophagus of weanling rats:A histochemical,quantitative and ultrastructural study

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World Journal of Gastroenterology 2007年第26期13卷 3598-3604页

作者： edson a liberti Ricardo BV Fontes Verginia M Fuggi Laura BM Maifrino Romeu R Souza Autonomic Nervous System Laboratory (VQM) Department of AnatomyInstitute of Biomedical SciencesUniversity of So Paulo So PauloBrazil

AIM: To evaluate the effects of protein deprivation on the myenteric plexus of the esophagus of weanling rats. METHODS: Pregnant female Wistar rats were divided into 2 groups: nourished (N),receiving normal diet,and undernourished (D),receiving a protein-deprived diet,which continued after birth. At twenty-one days of age,13 esophagi from each group were submitted to light microscopy and morphometrical analysis employing the NADH diaphorase,NADPH diaphorase and acetylcholinesterase techniques. Three other esophagi from each group were evaluated with transmission electron microscopy (TEM). RESULTS: In both the NADH- and the NADPH-reactive mounts,the neurons of the N mounts were more intensely stained,while in the D esophagi only the larger neurons were reactive. Many myenteric neurons of N were intensely reactive for AChE activity but only a few neurons of D exhibited these aspects. Ultrastructural analysis revealed that the granular reticulum of N showed large numbers of ribosomes aligned on the outer surface of its regularly arranged membrane while the ribosomes of D were disposed in clusters. The chromatin was more homogeneously scattered inside the neuron nucleus of N as well as the granular component of the nucleolus was evidently more developed in this group. Statistically significant differences between N and D groups were detected in the total estimated number of neurons stained by the NADPH technique. CONCLUSION: The morphological and quantitative data shows that feeding with protein-deprived diet in 21-d old rats induces a delay in the development of the myenteric neurons of the esophagus.

关键词： Enteric nervous system Myenteric plexus Nutrition Microscopy Electron Transmission Esopha-gus Rat Wistar

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Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

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World Journal of Gastroenterology 2010年第5期16卷 563-570页

作者： Flavio M Greggio Ricardo BV Fontes Laura B Maifrino Patricia Castelucci Romeu Rodrigues de Souza edson a liberti Autonomic Nervous System Laboratory (VQM) Department of Anatomy Instituto de Ciencias Biomedicas da Universidade de Sao Paulo 2415 Av Prof Lineu Prestes 05508-900 So Paulo Brazil

AIM:To evaluate effects of preand postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normalfed (N42), proteindeprived (D42), and proteinrecovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron ***: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the prof ile of nerve cell perikarya (ranging from 200 to 400 μm2).CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

关键词： Esophagus Enteric nervous system Myenteric plexus Proteins Light microscopy Transmission electron microscopy

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Effects of protein deprivation and re-feeding on P2X_2 receptors in enteric neurons

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World Journal of Gastroenterology 2010年第29期16卷 3651-3663页

作者： Rúbia Misawa Priscila Azevedo Girotti Márcia Sanae Mizuno edson Aparecido liberti John Barton Furness Patricia Castelucci Department of Anatomy Institute of Biomedical SciencesUniversity of So Paulo Department of Anatomy and Cell Biology University of Melbourne

AIM:To investigate the effects of malnutrition and refeeding on the P2X2 receptor,nitric oxide synthase(NOS),calretinin,calbindin and choline acetyltransferase(ChAT) in neurons of the rat ***:We analyzed the co-localization,numbers and sizes of P2X2-expressing neurons in relation to NOS-immunoreactive(IR),calbindin-IR,ChAT-IR,and calretinin-IR neurons of the myenteric and submucosal *** experimental groups consisted of:(1) rats maintained on normal feed throughout pregnancy until 42 d post-parturition(N);(2) rats deprived of protein throughout pregnancy and 42 d post-parturition(D);and(3) rats undernourished for 21 d post-parturition and then given a protein diet from days 22 to 42(DR).The myenteric and submucosal plexuses were evaluated by double labeling by immunohistochemical methods for P2X2 receptor,NOS,ChAT,calbindin and ***:We found similar P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of myenteric and submucosal neurons from the N,D and DR *** labeling of the myenteric plexus demonstrated that approximately 100% of NOS-IR,calbindin-IR,calretinin-IR and ChAT-IR neurons in all groups also expressed the P2X2 *** the submucosal plexus,the calretinin-IR,ChAT-IR and calbindin-IR neurons were nearly all immunoreactive for the P2X2 *** the myenteric plexus,there was a 19% increase in numbers per cm2 for P2X2 receptor-IR neurons,64% for NOS-IR,84% for calretinin-IR and 26% for ChAT-IR neurons in the D *** spatial density of calbindin-IR neurons,however,did not differ among the three *** submucosal neuronal density increased for calbindin-IR,calretinin-IR and ChAT-IR *** average size of neurons in the myenteric plexus neurons in the D group was less than that in the controls and,in the re-fed rats;there was a 34% reduction in size only for the calretinin-IR ***:This work demonstrates that expression of the P2X2 receptor is present in inhibitory,intrins

关键词： Chemical coding Myenteric neurons Submucosal neurons Undernutrition

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Morphological effects of autoclaved diet on the myenteric neurons of rats

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World Journal of Gastroenterology 2011年第43期17卷 4799-4803页

作者： Patrícia O Gonalez Naianne K Clebis Renata B Mari Karina M Gagliardo Sandra R Stabille Haroldo G Faria edson a liberti JoséRoberto Kfoury Jr Department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of So Paulo Department of Morphology of the Centre of Biomedical Sciences Federal University of Rio Grande do Norte Department of Biology Paranaense University Department of Morphology MaringáState University Central Vivarium MaringáState University Department of Anatomy Biomedical Sciences InstituteUniversity of So Paulo

AIM:To evaluate the effect of autoclaved diet on the jejunum neurons of the myenteric plexus of rats during their ***:The experimental groups were made up of rats going through weaning whose mothers received either an autoclaved or a non-autoclaved diet during gestation and lactation,and rats that were fed the same diet as their mothers during the post-weaning *** order to measure the neurons'body profile and to quantify the number of neurons per area,preparations were stained by the nicotinamide adenine dinucleotide-diaphorase ***:No significant changes were observed in rats'body weight or in the number of neurons regardless of the diet used(P>0.05) .There was a decrease in the jejunum-ileum length in rats treated with an autoclaved diet(P<0.05) .An increase in the neuronal cross-sectional area was seen in rats that had received the autoclaved diet,an effect that was significant for animals undergoing *** addition,all observed factors showed significant differences when related to the age of the ***:The autoclaved diet did not alter the quantity of neurons,but increased their cell body area,suggesting changes similar to those observed in protein deficiency.

关键词： Myenteric neurons Jejunum Morphometry Diet Autoclaving

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