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Temperature Inversions,Meteorological Variables and Air Pollutants and Their Influence on Acute Respiratory Disease in the Guadalajara Metropolitan Zone,jalisco,Mexico

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journal of environmental Protection 2013年第8期4卷 142-153页

作者： Hermes U.Ramirez-Sanchez Mario e.García-Guadalupe Hector H.Ulloa-Godinez Angel R.Meulenert-Pena Omar garcia-Concepcion jaime Alcala Gutierrez Sarahi j.Lizarraga Brito Department of Physics Institute of Astronomy and MeteorologyUniversity Center of Exact Sciences and Engineering(CUCEI)University of GuadalajaraGuadalajaraMexico

The presence of temperature inversions (TI), concentration of air pollutants (AP) and meteorological variables (MV) affect the welfare of the population, creating public health problems (acute respiratory diseases ARDs, among others). The Guadalajara Metropolitan Zone (GMZ) experiences high levels of air pollution, which associated with the presence of temperature inversions and meteorological variations is conducive to the incidence of ARDs in children. The aim of this work is to evaluate the TI, MV, AP and their influence on the ARDs in children under five years in the GMZ from 2003 to 2007. In this period, the moderate and strong TI are the most frequent presenting from November to May. The AP shows a variable behavior during the year and between years, with the highest concentration of particles less than 10 microns (PM10), followed by ozone (O3), nitrogen dioxide (NO2), nitrogen oxides (NOX), carbon monoxide (CO) and sulfur dioxide (SO2), the most affected areas are the southeast of the GMZ. Annual arithmetic mean is 213,510 ± 41,209 ARDs consultations. The most important diseases are acute respiratory infections (98.0%), followed by pneumonia and bronchopneumonia (1.1%), asthma and status asthmaticus (0.5%) and streptococcal pharyngitis and tonsillitis (0.4%). Months with most inquiries were from October to March, mainly in the southeast, south and center of the city, coinciding with high levels of AP. Statistical analysis shows that the TI have significant correlation with ARDs in three years, temperature (Temp) in two, relative humidity (RH) in two, wind speed (WS) in three, wind direction (WD) in two, while that air pollutants NOX and NO2 showed significant correlation with ARDs throughout the period. CO and SO2 showed significance in two years, while the PM10 and O3 in one.

关键词： Temperature Inversions Meteorological Variables Air Pollutants Acute Respiratory Diseases

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Filling the Gaps to Solve the extensin Puzzle

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Molecular Plant 2018年第5期11卷 645-658页

作者： eliana Marzol Cecilia Borassi Mauro Bringas Ana Sede Diana Rosa Rodriguez garcia Luciana Capece jose M. estevez Fundacion Instituto Leloir and Instituto de Investigaciones Bioquimicas de Buenos Aires (IIBBA-CO.NICer) Avenida Patricias Argentinas 435 Buenos AiresCP C1405BWE Argentina Departamento de Quimica Inorganica Analitica y Quimica Fisica Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires (INQUIMAE-CONICET)Buenos Aires CP C1428EGA Argentina Instituto de Investigaciones en Ingenierfa Genetica y Biologia Molecular Dr. Hector Torres (INGEBI-CONICET) Vuelta de Obligado 2490 Buenos Aires C1428ADNArgentina

extensins （eXTs） are highly repetitive plant O-glycoproteins that require several post-translational modifi- cations （PTMs） to become functional in plant cell walls. First, they are hydroxylated on contiguous proline residues; then they are O-glycosylated on hydroxyproline and serine. After secretion into the apoplast, O-glycosylated eXTs form a tridimensional network organized by inter- and intra-Tyr linkages. Recent studies have made significant progress in the identification of the enzymatic machinery required to process eXTs, which includes prolyl 4-hydroxylases, glycosyltransferases, papain-type cysteine endopeptidases, and peroxidases. eXTs are abundant in plant tissues and are particularly important in rapidly expanding root hairs and pollen tubes, which grow in a polar manner. Small changes in eXT PTMs affect fastgrowing cells, although the molecular mechanisms underlying this regulation are unknown. In this review, we highlight recent advances in our understanding of eXT modifications throughout the secretory pathway, eXT assembly in cell walls, and possible sensing mechanisms involving the Catharanthus roseus cell surface sensor receptor-like kinases located at the interface between the apoplast and the cytoplasmic side of the plasma membrane.

关键词： Arabidopsis thaliana cysteine endopeptidases extensins glycosyltransferases peroxidases prolyl 4-hydroxylases

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Towards predicting biochar impacts on plant-available soil nitrogen content

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Biochar 2022年第1期4卷 1040-1054页

作者： Claire L.Phillips Kylie M.Meyer Manuel garcia-jaramillo Clara S.Weidman Catherine e.stewart Thomas Wanzek Michael A.Grusak Donald W.Watts jeff Novak Kristin M.Trippe USDA-ARS National Laboratory for Agriculture and environment AmesIAUSA USDA-ARS Forage Seed and Cereal Research Unit CorvallisORUSA Department of environmental Toxicology Oregon State UniversityCorvallisORUSA Department of Crop and Soil Science Oregon State UniversityCorvallisORUSA USDA-ARS Soil Management and Sugarbeet Research Unit Ft.CollinsCOUSA USDA-ARS edward T.Schafer Agricultural Research Center FargoNDUSA USDA-ARS Coastal Plain Soil Waterand Plant Research CenterFlorenceSCUSA

Biochars can improve soil health but have been widely shown to reduce plant-available nitrogen(N)owing to their high carbon(C)content,which stimulates microbial ***,because biochars contain large amounts of C that are not microbially available,their total elemental C:N ratio does not correspond well with impacts on soil *** hypothesized that impacts on soil plant-available N would relate to biochar mineralizable-C(C_(min))content,and that C:N ratios of the mineralizable biochar component could provide a means for predicting conditions of net soil N-mineralization *** conducted two laboratory experiments,the first measuring biochar C_(min)from respiration of isotopically labeled barley biochars manufactured at 300,500,and 750℃,and the second characterizing C_(min)by proxy measurements for ten biochars from six feedstocks at several *** both experiments,soils were incubated with 2%biochar by mass to determine impacts to soil *** to expectation,all the biochars increased soil N-mineralization relative to unamended *** unexpected,higher temperature(500 and 700℃)barley biochars with less C_(min)stimulated more soil decomposition and more soil N-mineralization than a 350℃barley ***,across diverse biochar feedstocks and production methods,none of the biochar characteristics correlated with soil *** finding of improved soil N-mineralization adds complexity to the range of soil N responses that can be expected in response to biochar *** of the limited ability to predict soil N responses from biochar properties,users should monitor soil N to manage soil fertility.

关键词： C:N ratio Plant-available nitrogen Priming Respiration ^(13)C

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ReLaX:the Helmholtz International Beamline for extreme Fields high-intensity short-pulse laser driver for relativistic laser-matter interaction and strong-field science using the high energy density instrument at the european X-ray free electron laser fac

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High Power Laser Science and engineering 2021年第4期9卷 144-159页

作者： A.Laso garcia H.Höppner A.Pelka C.Bähtz e.Brambrink S.Di Dio Cafiso j.Dreyer S.Göde M.Hassan T.Kluge j.Liu M.Makita D.Möller M.Nakatsutsumi T.R.Preston G.Priebe H.-P.Schlenvoigt j.-P.Schwinkendorf M.šmíd A.-M.Talposi M.Toncian U.Zastrau U.Schramm T.e.Cowan T.Toncian Helmholtz-Zentrum Dresden-Rossendorf 01328 DresdenGermany european XFeL 22869 SchenefeldGermany extreme Light Infrastructure-Nuclear Physics and Faculty of Physics University of Bucharest077126 MagureleRomania Technische Universität Dresden 01062 DresdenGermany

High-energy and high-intensity lasers are essential for pushing the boundaries of *** development has allowed leaps forward in basic research areas,including laser±plasma interaction,high-energy density science,metrology,biology and medical *** Helmholtz International Beamline for extreme Fields user consortium contributes and operates two high-peak-power optical lasers using the high energy density instrument at the european X-ray free electron laser(euXFeL)*** lasers will be used to generate transient extreme states of density and temperature to be probed by the X-ray *** paper introduces the ReLaX laser,a short-pulse high-intensity Ti:Sa laser system,and discusses its characteristics as available for user *** will also present the first experimental commissioning results validating its successful integration into the euXFeL infrastructure and viability as a relativisticintensity laser driver.

关键词： X-ray free electron laser high-intensity laser relativistic intensity laser Ti:Sa laser

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Uncovering the genetic diversity of Giardia intestinalis in isolates from outbreaks in New Zealand

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Infectious Diseases of Poverty 2022年第3期11卷 27-36页

作者： Paul Ogbuigwe Patrick j.Biggs juan Carlos garcia-Ramirez Matthew A.Knox Anthony Pita Niluka Velathanthiri Nigel P.French David T.S.Hayman School of Veterinary Science Massey UniversityPalmerston NorthManawatu-WanganuiNew Zealand School of Natural Sciences Massey UniversityPalmerston NorthManawatu-WanganuiNew Zealand

Background:Giardia intestinalis is one of the most common causes of diarrhoea worldwide. Molecular techniques have greatly improved our understanding of the taxonomy and epidemiology of this parasite. Co-infection with mixed (sub-) assemblages has been reported, however, Sanger sequencing is sometimes unable to identify shared subtypes between samples involved in the same epidemiologically linked event, due to samples showing multiple dominant subtypes within the same outbreak. Here, we aimed to use a metabarcoding approach to uncover the genetic diversity within samples from sporadic and outbreak cases of giardiasis to characterise the subtype diversity, and determine if there are common sequences shared by epidemiologically linked cases that are missed by Sanger ***:We built a database with 1109 unique glutamate dehydrogenase (gdh) locus sequences covering most of the assemblages of G. intestinalis and used gdh metabarcoding to analyse 16 samples from sporadic and outbreak cases of giardiasis that occurred in New Zealand between 2010 and ***:There is considerable diversity of subtypes of G. intestinalis present in each sample. The utilisation of metabarcoding enabled the identification of shared subtypes between samples from the same outbreak. Multiple variants were identified in 13 of 16 samples, with Assemblage B variants most common, and Assemblages e and A present in mixed ***:This study showed that G. intestinalis infections in humans are frequently mixed, with multiple subtypes present in each host. Shared sequences among epidemiologically linked cases not identified through Sanger sequencing were detected. Considering the variation in symptoms observed in cases of giardiasis, and the potential link between symptoms and (sub-) assemblages, the frequency of mixed infections could have implications for our understanding of host–pathogen interactions.

关键词： DNA sequencing epidemiology Genetic diversity Giardiasis Metabarcoding

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Nanostructured biohybrid material with wide-ranging antiviral action

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Nano Research 2023年第8期16卷 11455-11463页

作者： Noelia Losada-garcia Angela Vazquez-Calvo David Ortega-Alarcon Olga Abian Adrian Velazquez-Campoy Pilar Domingo-Calap Antonio Alcami jose M.Palomo Instituto de Catálisis y Petroleoquímica(ICP) CSICC/Marie Curie 2.Madrid 28049Spain Centro de Biología Molecular Severo Ochoa Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Autónoma de Madrid(UAM)Madrid 28049Spain Institute of Biocomputation and Physics of Complex Systems(BIFI) Joint Unit GBsC-CSIC-BIFIUniversity of ZaragozaZaragoza 50018Spain Fundación Instituto de Investigación Sanitaria de Aragón(IIS Aragón) Zaragoza 50009Spain CIBeRehd Madrid 28029Spain Instituto de Biología Integrativa de Sistemas I2SyBioUniversitat de València-CSICValència E-46071Spain

Respiratory pathogens kill more people than any other infectious agent each year *** of novel,economically friendly,sustainable,and highly efficient materials against viruses is a major ***,we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized using a new biohybrid technology that employs a biological agent for its formation at room temperature in aqueous *** evaluation of different enzymes in the final preparation of the nanomaterial or even in synthetic methods was *** characterization revealed the formation of Cu species in the protein *** best biomaterial synthesized using a lipase called BioCuNPs showed excellent inhibition capacity against functional proteins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);for example,assent 3-chymotrypsin like protease(3CLpro)complete inhibition was achieved by using 5µg/mL,or acetone(ACe)–spike protein interaction was inhibited by more than 80%in the presence of 400µg/mL of *** these in vitro results into account,an efficacy analysis against human coronavirus 229e(HCoV-e229)coronavirus was performed.A virus reduction of 99%was obtained in 5 ***,SARS-CoV-2 virus was tested to demonstrate high efficiency,with>99%inhibition in 15 min using 500 microgram of *** determine the wide applicability of this nanohybrid against viruses,an evaluation was carried out against a non-enveloped virus such as Human Rhinovirus(HRV-14),obtaining a virus reduction of 99.9%in 5 ***,the virucidal capacity against different bacteriophages was also evaluated,obtaining an excellent inhibition effect against PhageΦX174(99.999%reduction in 5 min).

关键词： antiviral compound coronaviruses copper nanoparticles nanobiotechnology viruses

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Intensive outpatient comprehensive behavioral intervention for tics: A case series

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World journal of Clinical Cases 2014年第10期2卷 569-577页

作者： Tabatha H Blount Ann-Louise T Lockhart Rocio V garcia jeslina j Raj Alan L Peterson Department of Psychiatry The University of Texas Health Science Center at San AntonioSan Antonio TX 78229 United States Child and Adolescent Psychology San Antonio Military Medical Center Joint Base San Antonio Ft. Sam Houston TX 78234 United States

Recent randomized clinical trials have established the efficacy of Comprehensive Behavioral Intervention for Tics(CBIT) in treating children and adults with Tourette syndrome and persistent tic disorders. However, the standard CBIT protocol uses a weekly outpatient treatment format(i.e., 8 sessions over 10 wk), which may be inconvenient or impractical for some patients, particularly patients, who are required to travel long distances in order to receive care. In contrast, an intensive outpatient program may increase accessibility to evidence-based behavioral treatments for Tourette syndrome and other persistent tic disorders by eliminating the necessity of repeated travel. This case series evaluated the use of an intensive outpatient program CBIT(IOP CBIT) for the treatment of 2 preadolescent males(ages 10 and 14 years) with Tourette syndrome. The IOP CBIT treatment protocol included several hours of daily treatment over a 4-d period. Both children evi-denced notable reductions in their tics and maintained treatment gains at follow-up. Moreover, both patients and their parents expressed treatment satisfaction with the IOP CBIT format. This case series addresses an important research gap in the behavioral treatment of tic disorders literature. The patients' treatment outcomes indicate that IOP CBIT is a promising treatment that warrants more systematic investigation.

关键词： Tourette syndrome Tics Habit reversal Intensive outpatient Behavior therapy

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Gastric and small intestinal lipid digestion kinetics as affected by the gradual addition of lipases and bile salts

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Food Bioscience 2022年第2期46卷 781-792页

作者： S.H.e Verkempinck j.M.Guevara-Zambrano M.R.Infantes-garcia M.C.Naranjo R.Soliva-Fortuny P.elez-Martínez T.Grauwet KU Leuven Department of Microbial and Molecular Systems M2SLaboratory of Food Technology and Leuven Food Science and Nutrition Research Centre LFoRCeKasteelpark Arenberg 23PB 24573001LeuvenBelgium University of Lleida Department of Food TechnologyAgrotecnio CenterAv.Alcalde Rovira Roure 19125198LleidaSpain

We studied the effect of evolving from static in vitro digestion conditions towards the gradual addition of essential digestive compounds for lipid *** oil-in-water emulsions were considered:a low(5%w/w)and high(20%w/w)triolein-based emulsion with identical surfactant-to-oil ratio(0.2).emulsions were subjected to in vitro static digestion conditions or gradual gastric lipase addition,gradual pancreatic lipase addition,and/or gradual bile salt *** these three latter cases,similar amounts of gastric/pancreatic lipase and/or bile salts were provided as in the static case,however divided over 4 doses added during the first 30 min of each digestive *** the low-lipid emulsion,gradually adding lipases and bile salts did not significantly affect lipolysis *** can be related to the sufficient amounts of digestive compounds present even in the smallest initial *** the high-lipid emulsion,the gradual addition of bile salts significantly reduced the lipolysis *** salts are essential to remove lipid digestion products from the interface and thus allow the continuation of the lipid digestion process at the ***,the lipolysis extent after 2 h of small intestinal phase was not significantly influenced by the digestion *** is again explained by the simple nature of the emulsions studied and the excess of lipase even in the smallest initial ***,this work showed that evolving towards more(semi-)dynamic digestion conditions can impact(lipid)digestion kinetics,even for relatively simple food compositions,and is of interest to obtain more physiological relevant digestion kinetics.

关键词： Kinetics In vitro digestion Microemulsions Lipid digestion Lipases Bile salts

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Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

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journal of Integrative Medicine 2019年第2期17卷 132-140页

作者： Carlos R.Oliveira Daniel G.Spindola Daniel M.garcia Adolfo erustes Alexandre Bechara Caroline Palmeira-dos-Santos Soraya S.Smaili Gustavo j.S.Pereira André Hinsberger ezequiel P.Viriato Maria Cristina Marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz elaine G.Rodrigues Claudia Bincoletto Departamento de Farmacologia Escola Paulista de Medicina (EPM) Universidade Federal de Sao Paulo (UNIFESP) Grupo de Fitocomplexos e Sinalizacao Celular Escola de Ciências da Saúde Universidade Anhembi Morumbi Faculdade de Ciências Farmacêuticas e Bioquímicas Oswaldo Cruz Laboratório de Produtos Naturais e Quimiometria Programa de pós-graduacao em Farmácia e Biotecnologia Universidade Anhanguera de Sao Paulo Faculdade de Ciências Farmacêuticas Universidade Estadual de Campinas (UNICAMP) Unidade de Oncologia experimental EPM Universidade Federal de Sao Paulo (UNIFESP)

Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its *** study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CeAA) on breast ***: The cytotoxicity of CeAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CeAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo ***: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CeAA evaluated in this study(73 mg/mL). The CeAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CeAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CeAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CeAA-treated MCF7 cells. Gavage administration of the CeAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated ***: Altogether, our data show the antitumor potential of the CeAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CeAA in breast cancer therapy.

关键词： Angelica archangelica Umbelliferae Apoptosis Cell death Breast cancer Angelicin

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1.3 μm emitting SrF2：Nd^3＋ nanoparticles for high contrast in vivo imaging in the second biological window

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Nano Research 2015年第2期8卷 649-665页

作者： Irene Villa Anna Vedda Irene Xochilt Cantarelli Marco Pedroni Fabio Piccinelli Marco Bettinelli Adolfo Speghini Marta Quintanilla Fiorenzo Vetrone Ueslen Rocha Carlos jacinto elisa Carrasco Francisco Sanz Rodrfguez Angeles juarranz Blanca del Rosal Dirk H. Ortgies Patricia Haro Gonzalez jose garcia Sole Daniel jaque garcia Department of Materials Science University of Milano-Bicocca Via R. Cozzi 55 20125 Milano Italy Dipartimento di Biotecnologie Universita di Verona and INSTM UdR Verona Strada le Grazie 15 37134 Verona Italy Institut National de la Recherche Scientifique-energie Materiaux et Telecommunications Universitd du Quebec Varennes QC PQ J3X 1S2 Canada Grupo de Fotonica e Fluidos Complexos Instituto de Fisica Universidade Federal de Alagoas 57072-970 Maceio Alagoas Brazil Departamento de Biologia Facultad de Ciencias Campus de Cantoblanco Universidad Autdnoma de Madrid Madrid 28049 Spain Fluorescence Imaging Group Departamento de Fisica de Maferiales Facultad de Ciencias Campus de Cantoblanco Universidad Aut6noma de Madrid Madrid 28049 Spain

Novel approaches for high contrast, deep tissue, in vivo fluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window （1,000-1,400 nm）. This allows for the acquisition of high resolution, deep tissue images due to the partial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy （infrared） photons also leads to a drastic reduction in the contribution of autofluorescence to the in vivo image. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimens diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3. ions embedded in SrF2 nanoparticles can be used to produce autofluorescence free, high contrast in vivo fluorescence images. It is also dem- onstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies.

关键词： fluorescence imaging rare earth dopednanoparticles nanomedicine

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