检索结果-南通市图书馆

Synthesis and Anticancer Effect of gem-Difluoromethylenated Chrysin Derivatives

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第11期17卷 1439-1442页

作者： Xing ZHENG Jian Guo CAO duan fang liao Bing Yang ZHU Hui Ting LIU Institute of Pharmacy ＆ Pharmacology Nanhua University Hengyang 421001 Cancer Research Institute Nanhua University Hengyang 421001

Ten gem-difluoromethylenated chrysin derivatives were prepared and their anticancer activities in vitro were evaluated by the standard MTT method. The results of biological test showed that some of gem-difluoromethyle... 详细信息

关键词： Anticancer activity gem-difluoromethylenated chrysin.

Silencing of Bcl-XL Expression in Human MGC-803 Gastric Cancer Cells by siRNA

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第8期37卷 555-560页

作者： Xiao-Yong LEI~(#*) Miao ZHONG~# Lan-fang FENG Chun-Yan YAN Bing-Yang ZHU Sheng-Song TANG duan-fang liao Institute of Pharmacy and Pharmacology,Nanhua University,Hengyang 421001,China Institute of Pharmacy and Pharmacology Nanhua UniversityHengyang 421001 China

To investigate the inhibitory effect of the Bcl-XL small interfering RNA(siRNA)on BcI-XLgene expression in the human gastric cancer cell line MGC-803,green fluorescent protein(GFP)siRNAwas constructed and transfected into MGC-803 ceils,together with GFP expression vector pTrace *** expression levels were observed using fluorescence ***-XL siRNA and negative siRNAwere then constructed and stably transfected into MGC-803 ***-PCR and immunofluorescence wereused to detect the expression of *** apoptosis was detected by acridine orange(AO)andflow *** were as follows:(1)48 h after GFP expression vector and GFP siRNA co-transfection,the expression level of GFP in the GFP siRNA group was much lower than the negative siRNA group,according to fluorescence microscopy *** mRNA and protein levels of Bcl-XL in Bcl-XL siRNAstable transfectants were reduced to almost background level compared with negative siRNA transfectantsor untreated cells.(2)Changes in nucleus morphology was observed by AO staining nucleic and flowcytometry analysis,which showed that stable Bcl-XL siRNA transfectants have an increased spontaneousapoptosis (21.17%+1.26% vs.1.19%+0.18% and 1.56%+0.15% respectively,P<0.05 *** siRNAor untreated control),siRNA targeting GFP or Bcl-XL genes can specifically suppress GFP or BcI-XLexpression in MGC-803 cells,and Bcl-XL siRNA can increase spontaneous ***-XL siRNA maybe a beneficial agent against human gastric adenocarcinoma.

关键词： Bcl-XL siRNA MGC-803 cells

Triangle-free Graphs with Three Positive Eigenvalues

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

ALGEBRA COLLOQUIUM 2024年第3期31卷 525-540页

作者： duan, fang Xinjiang Normal Univ Sch Math Sci Urumqi 830017 Peoples R China

A graph G is called triangle-free if G does not contain any triangle as its induced subgraph. Let G(n) be the set of triangle-free graphs of order n each of which has three positive eigenvalues. In this paper, we find 20 specific graphs in G(n), each of which has nullity no more than 2, and we show that in terms of three graph transformations all the other graphs of G(n) can be constructed from these 20 specific graphs. Hence, we completely characterize the triangle-free graphs with exactly three positive eigenvalues.

关键词： positive (negative) inertia index nullity triangle-free graph SIGNED GRAPHS INDEX

Effect of Autophagy Over Liver Diseases

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2016年第1期31卷 65-68页

作者： Dong-qian Yi Xue-feng Yang duan-fang liao Qing Wu Nian Fu Yang Hu Ting Cao Department of Digestion Internal Medicine the Affiliated Nanhua Hospital of University of South China Department of Pathology Hunan University of Chinese Medicine

In recent years, increasingly evidences show that autophagy plays an important role in the pathogenesis and development of liver diseases, and the relationship between them has increasingly become a focus of concern. Autophagy refers to the process through which the impaired organelles, misfolded protein, and intruding microorganisms is degraded by lysosomes to maintain stability inside cells. This article states the effect of autophagy on liver diseases （hepatic fibrosis, fatty liver, viral hepatitis, and liver cancer）, which aims to provide a new direction for the treatment of liver diseases.

关键词： autophagy hepatic fibrosis fatty liver viral hepatitis liver cancer

On Hamiltonian Property of Cayley Digraphs

在线全文

学校读者我要写书评

暂无评论

Acta Mathematicae Applicatae Sinica 2024年第2期40卷 547-556页

作者： fang duan Qiong-xiang HUANG School of Mathematics Science Xinjiang Normal UniversityUrumqi 830017China College of Mathematics and Systems Science Xinjiang UniversityUrumqi 830017China

Let G be a finite group generated by S and C(G,S) the Cayley digraphs of G with connection set *** this paper,we give some sufficient conditions for the existence of hamiltonian circuit in C(G,S),where G=Zm×H is a semiproduct of Zmby a subgroup H of *** particular,if m is a prime,then the Cayley digraph of G has a hamiltonian circuit unless G=Zm×*** addition,we introduce a new digraph operation,called φ-semiproduct of Γ1by Γ2and denoted by Γ1×Γ_φΓ2,in terms of mapping φ:V(Γ2)→{1,-1}.Furthermore we prove that C(Zm,{a})×_φ C(H,S) is also a Cayley digraph if φ is a homomorphism from H to{1,-1} ≤ Zm~*,which produces some classes of Cayley digraphs that have hamiltonian circuits.

关键词： Cayley digraphs hamiltonian circuit external semidirect product

Onychin inhibits proliferation of vascular smooth muscle cells by regulating cell cycle

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第2期26卷 205-211页

作者： Ming YANG~2,Hong-lin HUANG~2,Bing-yang ZHU,Qin-hui TUO,duan-fang liao~3Institute of Pharmacy and Pharmacology,Nanhua University,Hengyang 421001,China InstituteofPharmacyandPharmacology NanhuaUniversityHengyang421001China

Aim:To investigate the effects of onychin on the proliferation of cultured ratartery vascular smooth muscle cells(VSMCs)in the presence of 10% new-borncalf serum(NCS).Methods:Rat VSMCs were incubated with onychin 1-50μmol/L or genistein 10μmol/L in the presence of 10% NCS for 24 *** prolif-eration of VSMCs was measured by cell counting and MTS/PMS *** cycle progression was evaluated by flow ***(Rb)phosphorylation,and expression of cyclin D1and cyclin E were measured byWestern blot *** tyrosine phosphorylation of ERK1/2 was examined byimmunoprecipitation techniques using anti-phospho-tyrosine ***:The proliferation of VSMCs was accelerated significantly in the presence of 10%*** reduced the metabolic rate of MTS and the cell number of VSMCsin the presence of 10% NCS in a dose-dependent *** cytometry analy-sis revealed that the G1-phase fraction ratio in the onychin group was higher thanthat in the 10% NCS group(85.2% vs 70.0%,P<0.01),while the S-phase fractionratio in the onychin group was lower than that in 10% NCS group(4.3% vs16.4%,P<0.01).Western blot analysis showed that onychin inhibited Rb phos-phorylation and reduced the expression of cyclin D1and cyclin *** effects ofonychin on proliferation,the cell cycle and the expression of cyclins in VSMCswere similar to those of genistein,an inhibitor of tyrosine *** studies showed that both onychin and genistein markedlyinhibited the tyrosine phosphorylation of ERK1/2 induced by 10% ***:Onychin inhibits the proliferation of VSMCs through G1phase cellcycle arrest by decreasing the tyrosine phosphorylation of ERK1/2,and the ex-pression of cyclin D1and cyclin E,and sequentially inhibiting Rb phosphorylation.

关键词： onych(?)n vascular smooth muscle cell cycle retinoblastoma cyclin D1 cyclin E tyrosine kinase mitogen-activated protein kmase

BcI-XL Small Interfering RNA Enhances Sensitivity of Hepg2 Hepatocellular Carcinoma Cells to 5-Fluorouracil and Hydroxycamptothecin

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第10期 704-710页

作者： Xiao-Yong LEI* Miao ZHONG Lan-fang FENG Bing-Yang ZHU Sheng-Song TANG duan-fang liao Institute of Pharmacy and Pharmacology,Nanhua University,Hengyang 421001,China

Changes in drug sensitivity in Bcl-XL small interfering RNA (siRNA) transfected Hepg2hepatocellular carcinoma cells were investigated in this ***-XL siRNA and negative siRNA expressionvector were constructed and stably transfected into Hepg2 *** transcription (RT)-PCR,westernblot and immunofluorescence were used to detect the target gene expression at mRNA and protein *** sensitivity of the cells to 5-fluorouracil (5-FU) and hydroxycamptothecin (HCPT) were evaluated *** Bcl-XL mRNA and protein expression levels in Bcl-XL siRNA transfectants were reducedcompared with negative siRNA transfectants or mock *** results showed that Bcl-XL siRNAtransfected cells have a higher cell inhibition rate than negative vector transfected cells or untreated cellsafter treatment with 13,130,1300 and 13,000 mg/L of ***-XL siRNA transfected cells also showedincreased drug-sensitivity compared with negative vector transfected cells or untreated cells after treatmentwith 0.18,0.36,0.72 and 1.44 mg/L *** cytometry (FCM) results demonstrated that the sub-G1population increased in the Bcl-XL siRNA group,compared with the negative siRNA group and untreatedcontrol group,after the addition of 5-FU (1300 mg/L) and HCPT (0.72 mg/L),siRNA targeting Bcl-XL genecan specifically down-regulate Bcl-XL expression in Hepg2 cells,and can increase spontaneous cell apoptosisand sensitize cells to 5-FU or HCPT.

关键词： Bcl-XL small interference RNA Hepg2 cells 5-fluorouracil hydroxycamptothecin

KeyEE:Enhancing Low-Resource Generative Event Extraction with Auxiliary Keyword Sub-Prompt

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Big Data Mining and Analytics 2024年第2期7卷 547-560页

作者： Junwen duan Xincheng liao Ying An Jianxin Wang Hunan Key Laboratory of Bioinformatics School of Computer Science and EngineeringCentral South UniversityChangsha 410083China Big Data Institute Central South UniversityChangsha 410083China

Event Extraction(EE)is a key task in information extraction,which requires high-quality annotated data that are often costly to *** classification-based methods suffer from low-resource scenarios due to the lack of label semantics and fine-grained *** recent approaches have endeavored to address EE through a more data-efficient generative process,they often overlook event keywords,which are vital for *** tackle these challenges,we introduce KeyEE,a multi-prompt learning strategy that improves low-resource event extraction by Event Keywords Extraction(EKE).We suggest employing an auxiliary EKE sub-prompt and concurrently training both EE and EKE with a shared pre-trained language *** the auxiliary sub-prompt,KeyEE learns event keywords knowledge implicitly,thereby reducing the dependence on annotated ***,we investigate and analyze various EKE sub-prompt strategies to encourage further research in this *** experiments on benchmark datasets ACE2005 and ERE show that KeyEE achieves significant improvement in low-resource settings and sets new state-of-the-art results.

关键词： natural language processing Event Extraction(EE) Multi-Prompt Learning(MPL) low-resource

Removal of Cr(Ⅵ)by glutaraldehyde-crosslinked chitosan encapsulating microscale zero-valent iron:Synthesis,mechanism,and longevity

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第8期142卷 115-128页

作者： Yijun duan fang Liu Xiang Liu Miao Li School of Environment Tsinghua UniversityBeijing 100084China Transportation Institute of Inner Mongolia University Hohhot 010070China

Microscale zero-valent iron(mZVI)has shown great potential for groundwater Cr(Ⅵ)***,low Cr(Ⅵ)removal capacity caused by passivation restricted the wide use of *** prepared mZVI/GCS by encapsulating mZVI in a porous glutaraldehydecrosslinked chitosan matrix,and the formation of the passivation layer was alleviated by reducing the contact between zero-valent iron *** average pore diameter of mZVI/GCS was 8.775 nm,which confirmed the mesoporous characteristic of this *** of batch experiments demonstrated that mZVI/GCS exhibited high Cr(Ⅵ)removal efficiency in a wide range of pH(2-10)and temperature(5-35℃).Common groundwater coexisting ions slightly affected m ZVI/*** material showed great reusability,and the average Cr(Ⅵ)removal efficiency was 90.41%during eight *** this study,we also conducted kinetics and isotherms ***-second-order model was the most matched kinetics *** Cr(Ⅵ)adsorption process was fitted by both Langmuir and Freundlich isotherms models,and the maximum Langmuir adsorption capacity of m ZVI/GCS reached243.63 mg/g,which is higher than the adsorption capacities of materials reported in most of the previous ***,the column capacity for Cr(Ⅵ)removal of a mZVI/GCS-packed column was 6.4 times higher than that of a mZVI-packed column in a 50-day ***,mZVI/GCS with a porous structure effectively relieved passivation problems of mZVI and showed practical application prospects as groundwater Cr(Ⅵ)remediation material with practical application prospects.

关键词： Chitosan Glutaraldehyde mZVI Cr(Ⅵ) Groundwater