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煤炭技术 2012年第5期31卷 259-260页

作者：郭东峰新乡学院河南新乡453000

该系统基本实现网上留言的动作过程,同时提供了后台管理的功能,可方便的对前台的界面、用户的留言及与本留言薄相关的信息进行动态管理。

关键词：网络留言薄系统设计

脓毒症患者血浆微RNA-146a表达与患病风险、炎性因子水平及预后的相关性分析

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第二军医大学学报 2020年第2期41卷 135-140页

作者：王华王莉郭东风海军军医大学(第二军医大学)附属公利医院急诊科上海200135 上海中医药大学附属第七人民医院皮肤科上海200137

目的评估脓毒症患者血浆miRNA-146a表达水平对患病风险及预后的价值。方法连续纳入2015年10月至2017年3月于海军军医大学(第二军医大学)附属公利医院急诊科和ICU就诊的132例脓毒症患者,以及同期在健康体检中心进行健康体检的131名健康... 详细信息

目的评估脓毒症患者血浆miRNA-146a表达水平对患病风险及预后的价值。方法连续纳入2015年10月至2017年3月于海军军医大学(第二军医大学)附属公利医院急诊科和ICU就诊的132例脓毒症患者,以及同期在健康体检中心进行健康体检的131名健康对照。采集两组研究对象的血液样本,采用qRT-PCR检测血浆miRNA-146a表达,采用ELISA法测定血清炎性因子(CRP、TNF-α、IL-1β、IL-6、IL-8、IL-10)水平。结果与健康对照相比,脓毒症患者血浆中miRNA-146a表达水平较低(Z=-7.423,P<0.01);ROC曲线显示血浆miRNA-146a对脓毒症有较好的诊断价值,AUC为0.765(95%CI 0.707~0.823),取约登指数最大值处的界值为最佳诊断界值,此时灵敏度和特异度分别为87.9%和61.8%。多元logistic回归模型显示血浆miRNA-146a表达水平低是脓毒症患病风险的独立预测因素(OR=0.312,95%CI 0.225~0.434,P<0.01)。Spearman相关分析显示血浆miRNA-146a表达水平与急性生理功能和慢性健康状况Ⅱ(APACHEⅡ)评分呈负相关(r=-0.562,P<0.01),与血清CRP(r=-0.304,P<0.001)、TNF-α(r=-0.287,P=0.001)、IL-1β(r=-0.173,P=0.047)及IL-6(r=-0.213,P=0.014)呈负相关,与血清IL-10(r=0.225,P=0.009)呈正相关。与存活脓毒症患者相比,死亡患者血浆中miRNA-146a的表达水平降低(Z=-2.818,P=0.005)。ROC曲线分析显示血浆miRNA-146a对脓毒症有良好的预后价值,AUC为0.660(95%CI 0.546~0.773),最佳诊断界值的灵敏度和特异度分别为88.5%和47.2%。结论血浆miRNA-146a表达水平低是脓毒症患病的独立预测因素,并与疾病严重程度高、炎性因子水平高及预后不良有关。

关键词：微RNA-146a 脓毒症危险因素预后炎性因子

Remote Sensing CO,CO_2 in Vehicle Emissions Based on TDLAS

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光机电信息 2007年第5期24卷 25-30页

作者： WANG Tie-dong LIU Wen-qing ZHANG Yu-jun LIU Jian-guo dong feng-zhong WANG Min WANG Xiao-mei XU Min Key Laboratory of Environment Optics and Technology Anhui Institute of Optics and Fine Mechanics Chinese Academy of SciencesHefei 230031China

Knowing the quantity of pollutants that the vehicle fleet is emitting to the air has become a vital problem in almost every major city in China. Finding and fixing gross polluters is therefore very important to control the urban air quality and protect the human health and the environment. Remote sensing is an important advance in the technology of on-road vehicle emissions testing because it is fast, mobile, and unobtrusive. This on-road vehicle emissions remote system is designed to measure the carbon monoxide, carbon dioxide and opacity from the vehicles's tailpipe based on the Tunable Diode Laser Absorption Spectroscopy (TDLAS). There are several advantages of this system such as compact design and easy of use. The measurement principle and optical layout of the instrument has been described in this paper. Field testing at Beijing and Hefei were conducted over one year, more than 6 000 vehicles were tested. This vehicle emissions remote system has been shown to be able to measure CO,CO2 and opacity from individual at highway speeds. In parallel, the plate license, speed, acceleration and length of vehicle are recognised by computer so that the owners of vehicles exceeding the permissible level of emissions can be identified.

关键词：车辆排放一氧化碳排放二氧化碳排放遥感 TDLAS

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寄生植物肉苁蓉及寄主微量元素的含量研究

光谱学与光谱分析 2011年第4期31卷 1030-1032页

作者：黄勇郭东锋骆翔赵东平朱艳霞郭玉海中国农业大学农学与生物技术学院北京100193

随着寄生植物对我国农林业危害越来越来大,寄生植物与寄主间相互作用及物质转运的研究也成为热点。借助先进的ICP-AES技术对寄生植物肉苁蓉及其寄主不同器官中的各种微量元素进行了系统的研究,结果发现肉苁蓉及其寄主中所含主要元素基... 详细信息

随着寄生植物对我国农林业危害越来越来大,寄生植物与寄主间相互作用及物质转运的研究也成为热点。借助先进的ICP-AES技术对寄生植物肉苁蓉及其寄主不同器官中的各种微量元素进行了系统的研究,结果发现肉苁蓉及其寄主中所含主要元素基本相同,但各种元素含量存在一定的差异,其中K,P,Na,Ca元素含量较高,且肉苁蓉中K和P含量高于寄主植物。研究结果为寄生植物合理利用及有害寄生杂草防除提供了新的科学依据。

关键词： ICP-AES 寄生植物微量元素

非局域克尔介质中厄米高斯光束传输的变分研究

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物理学报 2008年第9期57卷 5684-5689页

作者：白东峰郭旗胡巍华南师范大学光子信息技术广东省高校重点实验室广州510006

在非局域非线性克尔介质中,通过对介质实对称响应函数的泰勒展开,简化了非局域非线性薛定谔方程所对应的Lagrange密度,进而利用变分法对光束的传输问题进行了分析.求出试探解各个参量的演化方程并得到了自聚焦介质中的厄米高斯型光束的... 详细信息

在非局域非线性克尔介质中,通过对介质实对称响应函数的泰勒展开,简化了非局域非线性薛定谔方程所对应的Lagrange密度,进而利用变分法对光束的传输问题进行了分析.求出试探解各个参量的演化方程并得到了自聚焦介质中的厄米高斯型光束的精确解析解,当输入功率达到临界功率时,即形成高阶空间光孤子(厄米高斯孤子),其最低阶(基模光孤子)就是高斯孤子.通过数值模拟发现解析解与数值解符合得很好.

关键词：非局域克尔介质变分法厄米高斯光束空间光孤子

Different Neuroprotection and Therapeutic Time Windows by Two Specific Diazepam Regimens on Retinal Ganglion Cells after Optic Nerve Transection in Adult Rats

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Different Neuroprotection and Therapeutic Time Windows by Tw...

第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Ling Liu Xi-Ying Jiao guo-dong feng Ming-Mei Wu An-An Yang Si-Wei You Institute of Neurosciences the Fourth Military Medical University

Purpose: To compare neuroprotection and therapeutic time windows of two diazepam regimens on retinal ganglion cells (RGCs) after rat optic nerve transection (ONT). Methods: Adult rats received initial intraperitoneal diazepam injections 30 minutes before left ONT, followed by daily diazepam (Regimen-A) or every 8 hours for 3 days (Regimen-B) until they were killed at Day 7 or 14. Initial diazepam in Regimen-A and Regimen-B was delayed to 3,

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针灸标准化与针灸知识产权相互关系研究

针刺研究 2014年第5期39卷 427-430页

作者：董国锋武晓冬韩焱晶中国中医科学院针灸研究所北京100700

知识经济条件下,针灸标准化必然涉及知识产权。既然如此,针灸标准化与针灸知识产权二者间到底是什么关系呢?二者间的关系就是既排斥又融合的关系。如果处理不好二者间的关系,不仅会给二者各自的发展造成不利影响,还会对针灸事业的发展... 详细信息

知识经济条件下,针灸标准化必然涉及知识产权。既然如此,针灸标准化与针灸知识产权二者间到底是什么关系呢?二者间的关系就是既排斥又融合的关系。如果处理不好二者间的关系,不仅会给二者各自的发展造成不利影响,还会对针灸事业的发展与繁荣造成不利影响。因此,恰当处理二者的关系,对解决诸如此类的问题具有重要现实意义。而恰当处理二者关系的前提是,必须先明了二者间的关系。本文对二者的异同做了系统比较,并对二者的相互作用做了全面剖析。

关键词：针灸疗法标准化知识产权相互关系

Effect of antisense DNMT3b gene eukaryotic expression plasmid on expression of the DNMT3b gene in human biliary tract carcinoma cells

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Hepatobiliary & Pancreatic Diseases International 2006年第1期5卷 123-128页

作者： Shi Zuo, Jing-Qing dong, Wei guo, Min-feng Liu, Li-Ning Xu, Jian Luo and Sheng-Quan Zou Department of General Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China Department of General Surgery Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 China.

BACKGROUND: Hypermethylation of the promoter region is one of the major mechanisms of tumor suppressor gene inactivation. DNA methyltransferase 3b (DNMT3b), an enzyme that participates in the establishment of de novo methylation patterns, is highly expressed in many tumor cells and tissues, and it is closely associated with hypermethylation of the promoter of tumor suppressor genes. The aim of this study was to explore the effect of transfection with antisense DNMT3b gene eukaryotic expression plasmid on the expression of the DNMT3b gene in human biliary tract carcinoma cell. METHODS: The constructed antisense DNMT3b gene eukaryotic expression plasmid was transfected into the human biliary tract carcinoma cell line QBC-939 with lipofectamine transfection reagent, and positive cell clones were formed using G418 selection after transfection. The constructed recombinant plasmid was transfected into QBC-939 cells successfully and was confirmed by amplification of the exogenous neo^R gene with the polymerase chain reaction method. The expression of DNMT3b gene mRNA and protein was detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry respectively. RESULTS: Following transfection, the mRNA level of the DNMT3b gene decreased from 0.956±0.053 to 0.209±0.023, and the protein level of the DNMT3b gene also decreased from (75.38±3.22)% to (29.87±3.46)%. Very significant differences were observed both at the transcription and posttranscription levels in the expression of the DNMT3b gene between the non-tranfection group and the antisense DN- MT3b gene eukaryotic expression plasmid transfection group (P<0.01). CONCLUSIONS: Transfection with the antisense DNMT3b gene eukaryotic expression plasmid can significantly reduce the expression level of the DNMT3b gene in the human biliary tract carcinoma cell line QBC-939. This study may provide a valid method to investigate the function of the DNMT3b gene and its role in biliary tract c

关键词： DNA methyltransferase 3b antisense RNA transfection gene expression biliary tract carcinoma

Identification of Dual Receptor-binding Specific Strains of Human H5N1 Viruses in China

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Biomedical and Environmental Sciences 2012年第1期25卷 104-108页

作者： ZHOU Jian Fang ZOU Shu Mei LI Zi WANG Min dong Jie guo Jun feng WEI He Jiang WEN Le Ying XU Hong SHU Yue Long State Key Laboratory for Molecular Virology and Genetic Engineering National Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China

Objective Both the 2, 6 linkage and its topology on target cells are critical for the recognition by human influenza virus. The binding preference of avian flu virus H5N1 HA to the 2, 3-1inked sialylated glycans is considered the major factor limiting its efficient infection and transmission in humans. To monitor potential adaptation of H5N1 virus in human population, the surveillance of receptor-binding specificity was undertaken in China. Methods The binding specificity of 32 human H5N1 virus strains isolated from 2003 to 2009 was tested by 2, 3-specific sialidase-treated chicken red blood cell （CRBC） agglutination assay and a solid-phase direct binding assay with synthetic sialylglycopolymers. Results Dual binding preference to 2, 3 and 2, 6-glycans were found in two strains： A/Guangdong/1/06 （A/GD/1/06） and A/Guangxi/1/08 （A/GX/1/08）. Though minor effect of short-2, 6-binding was detected in A/GX/1/08 at a low virus titer, both showed high affinity to the oligosaccharide at a high load. Notably both are of the long-2, 6-recognition, with the same topology as that of human H1N1 and H3N2 viruses. Conclusion The findings suggest that human H5N1 virus in China likely acquired the potential human-adaptation ability. Further research and surveillance on receptor-binding specificity of H5N1 viruses are required.

关键词： Receptor-binding specificity Human H5N2 virus China

CaH2-promoted activity of Ni-carbonate interface for CO2 methanation

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Journal of Energy Chemistry 2025年第1期100卷 522-532页

作者： Jin-Peng Wang guo-Cui Mao Hui-Lin Jiang Bao-Xia dong Yun-Lei Teng School of Chemistry and Chemical Engineering Yangzhou University

Transition metal-carbonate interfaces often act as active sites in heterogeneous catalytic *** interface between transition metal and metal carbonate exhibits a dynamic equilibrium during the CO2hydrogenation reaction,involving surface carbonate hydrogenation and ***,there have been few reports on engineering the activity of the interface between transition metal and alkaline earth metal carbonate for catalytic *** work demonstrated that the incorporation of CaH2in Ni/CaCO3enhances the CO2methanation activity of the *** CO2conversion for Ni/CaH2-CaCO3reached 68.5%at 400°C,which was much higher than that of the Ni/CaCO3（31.6%） and Ni/CaH2-CaO （42.4%） ***,the Ni/CaH2-CaCO3catalysts remained stable during the stability test for 24 h at 400°C and 8 *** research revealed that CaH2played a crucial role in promoting the activity of the Ni-carbonate interface for ***2could modify the electronic structure of Ni and tune the structural properties of CaCO3to generate medium basic sites （OH groups）,which are favorable for the activation of H2and ***-situ Fourier transform infrared spectroscopy （FTIR） analysis combined with density functional theory calculations demonstrated that CO2activation occurs at the hydroxyl group （OH） on the CaH2-modified Ni-carbonate surface,leading to the formation of CO3H****,our study has confirmed that CO2methanation over the Ni/CaH2-CaCO3catalysts proceeds via the formate pathway.

关键词： Metal hydride Ni catalysts Carbonate Metal-carbonate interface