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Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinofl agellate bloom

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Journal of Oceanology and Limnology 2021年第1期39卷 242-258页

作者： Jingyi CEN Jianyan WANG Lifen HUANG Yarou LIN guangmao ding Yuzao QI Songhui LÜ Research Center of Harmful Algae and Marine Biology Jinan UniversityGuangzhou 510632China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519000China Department of Science Research Beijing Museum of Natural HistoryBeijing 100050China Fujian Fishery Resources Monitoring Center Fuzhou 350003China Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes Jinan UniversityGuangzhou 510632China

Abstract On May 24–29,2019,a harmful algal bloom occurred in Pingtan coastal areas,Fujian,southeast China,and caused mass mortality of cage-cultured fi *** clonal cultures of an unknown naked dinofl agellate were set up from seawater samples taken during the *** cultures were examined for morphological features,ultrastructure characters,photosynthetic pigments,the large subunit(LSU)of the rRNA gene,and the internal transcribed spacer(ITS)sequences,as well as acute toxicity *** cell was unarmored,small-sized,and ovoid,and was characterized by elaborate striations on the epicone and *** nucleus was large,ellipsoid to oval or kidney-shaped,and centrally located in the cell.A long linear apical groove originated above the sulcus in the ventral epicone and extended to the dorsal *** elongate,slit-like“ventral pore”was located on the left of the epicone,well away from the apical *** chloroplasts were yellowish brown,numerous,band like,and irregularly distributed in the cell *** was the main accessory pigment *** topology reconstructed on partial LSU rDNA showed that the unknown dinofl agellate branched as a sister species to Karlodinium sp.(strain IFR981&IFR797,from France)and Karlodinium corrugatum(strain KDGSO08,from Australia),with genetic divergences of 0.6%and 3.3%,*** on the morphological and molecular phylogenetic analysis,we describe the novel dinofl agellate as Karlodinium elegans ***.A toxicity assay revealed that the clonal culture of ***(strain PTB601)had no adverse eff ect on brine shrimp(Artemia salina)and marine medaka(Oryzias melastigma),indicating it may not be a toxic species.

关键词： Karlodinium Kareniaceae harmful algal bloom toxicity dinofl agellate

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Who is the“murderer”of the bloom in coastal waters of Fujian,China,in 2019?

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Journal of Oceanology and Limnology 2020年第3期38卷 722-732页

作者： CEN Jingyi WANG Jianyan HUANG Lifen ding guangmao QI Yuzao CAO Rongbo CUI Lei Lü Songhui College of Life Science and Technology Jinan UniversityGuangzhou 510632China Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes Guangzhou 510632China Department of Science Research Beijing Museum of Natural HistoryBeijing 100050China Fujian Fishery Resources Monitoring Center Fuzhou 350003China

On May 24-29,2019,a bloom occurring in Pingtan coastal areas of Fujian Province caused mass mortality of cage-cultured fish(Plectorhinchus cinctus and Pagrosomus major).During the bloom,two major causative organisms were present:Prorocentrum donghaiense(at a concentration of 1.46×10^7 cells/L)and an unknown naked dinoflagellate(4.58×10^6 cells/L).The naked dinoflagellate was isolated and cultured in this study,and its morphological features were examined using light microscopy and scanning electron *** large subunit(LSU)of the rRNA gene and the internal transcribed spacer(ITS)region of the naked dinoflagellate were also sequenced for field bloom samples and lab culture strains(PT-A and PT-B).On the basis of its morphological characteristics and molecular sequences,the unknown naked dinoflagellate was identified as Karlodinium *** to the phylogenetic analysis,the *** was most closely related to Karlodinium australe and Karlodinium armiger,and the three species clustered into a single clade of Karlodinium with bootstrap/posterior probability values of 95%/0.99 and 86%/0.99 inferred from LSU and ITS sequences,*** was first reported as Karenia digitata,a new harmful algal species bloomed in Hong Kong,China,in *** present study,we gave a detailed morphological and phylogenetic description of *** and submitted the molecular sequences of this species to GenBank for the first time.

关键词： Karenia digitata Karlodinium digitatum harmful algal bloom Ichthyotoxicity

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Comparison of short-term toxicity of 14 common phycotoxins(alone and in combination)to the survival of brine shrimp Artemia salina

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Acta Oceanologica Sinica 2023年第2期42卷 134-141页

作者： Yuting Zhang Shanshan Song Bin Zhang Yang Zhang Miao Tian Ziyi Wu Huorong Chen guangmao ding Renyan Liu Jingli Mu Institute of Oceanography College of Geography and OceanographyMinjiang UniversityFuzhou350108China Fuzhou Institute of Oceanography Fuzhou350108China Department of Marine Chemistry National Marine Environmental Monitoring CenterDalian116023China Fujian Marine Environment and Fishery Resource Monitoring Center Fuzhou350003China

Toxic harmful algal blooms(HABs)can cause deleterious effects in marine organisms,threatening the stability of marine *** is well known that different strains,natural populations and growth conditions of the same toxic algal species may lead to different amount of phycotoxin production and the ensuing *** fully assess the ecological risk of toxic HABs,it is of great importance to investigate the toxic effects of phycotoxins in marine *** this study,the short-term toxicity of 14 common phycotoxins(alone and in combination)in the marine zooplankton Artemia salina was *** 48 h LC_(50)of the 14 phycotoxins varied from 0.0193µg/mL to 2.415µg/*** most potent phycotoxin was azaspiracids-3(AZA3;with a LC_(50)of 0.0193µg/mL),followed by azaspiracids-2(AZA2;0.0226µg/mL),pectenotoxin-2(PTX2;0.0460µg/mL)and dinophysistoxin-1(DTX1;0.0818µg/mL).For the binary exposure,okadaic acid(OA)induced potential additive effects with DTX1,probably due to their similar structure(polyether fatty acid)and mode of action(attacking the serine/threonine phosphoprotein phosphatases).On the other hand,OA showed potential antagonistic effects with PTX2,which might be accounted for by their activation on the detoxification activity of cytochrome P450 *** addition,DTX1 induced potential synergetic effects with saxitoxin(STX),yessotoxin(YTX)or PTX2,suggesting the hazard potency of the mixtures of DTX1 and other phycotoxins(like STX,YTX and PTX2)with regard to the ecological *** results provide valuable toxicological data for assessing the impact of phycotoxins on marine planktonic species and highlight the potential ecological risk of toxic HABs in marine ecosystems.

关键词： LC_(50) harmful algal blooms binary exposure ecological risk

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2012年三沙湾米氏凯伦藻赤潮的生态特征及成因分析

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海洋学报 2018年第6期40卷 104-112页

作者：丁光茂张树峰福建省海洋环境与渔业资源监测中心福建福州350003 厦门大学环境与生态学院近海海洋环境科学国家重点实验室福建厦门361102

为了理清米氏凯伦藻赤潮形成与环境因素的关系,掌握其发生、发展规律,本文分析了2012年春末夏初三沙湾海域米氏凯伦藻赤潮期间的气象、水文和营养盐情况,并结合室内模拟培养实验,探讨了米氏凯伦藻赤潮形成与各环境因素的相关性。结果显... 详细信息

为了理清米氏凯伦藻赤潮形成与环境因素的关系,掌握其发生、发展规律,本文分析了2012年春末夏初三沙湾海域米氏凯伦藻赤潮期间的气象、水文和营养盐情况,并结合室内模拟培养实验,探讨了米氏凯伦藻赤潮形成与各环境因素的相关性。结果显示米氏凯伦藻赤潮通常发生在温度为20.5~25.0℃和盐度为24.3~32.3的海域,其细胞生长的最适温度和最适盐度分别为:22.0~25.0℃和26.0~30.0;Pearson相关性分析显示,米氏凯伦藻细胞密度与叶绿素a、无机磷、化学需氧量呈极显著正相关,与透明度、氨氮呈显著负相关;另外,米氏凯伦藻对磷酸盐的需求较低,其赤潮的形成可能主要受水体中氨氮的影响。基于现场调查和模拟实验结果,综合分析认为稳定的水文和气象条件、发生前期降雨输入的无机氮,以及由于持续阴雨导致的较低光照条件是此次米氏凯伦藻赤潮形成和维持的重要条件。

关键词：三沙湾米氏凯伦藻赤潮环境因素成因

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链状亚历山大藻谷氨酰胺合成酶的分离纯化及酶学特性研究

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渔业研究 2018年第2期40卷 98-103页

作者：丁光茂福建省海洋环境与渔业资源监测中心福建福州350003

本文以链状亚历山大藻(Alexandrium catenella)为实验材料,通过离子交换、分子筛和疏水层析等纯化技术来分离纯化谷氨酰胺合成酶(Glutamine synthetase,GS),并对GS的理化特性进行研究。研究结果表明,GS分子量为430 kD,由8个分子量为55.6... 详细信息

本文以链状亚历山大藻(Alexandrium catenella)为实验材料,通过离子交换、分子筛和疏水层析等纯化技术来分离纯化谷氨酰胺合成酶(Glutamine synthetase,GS),并对GS的理化特性进行研究。研究结果表明,GS分子量为430 kD,由8个分子量为55.6 kD的亚基组成;按照亚基分子量的大小判断,链状亚历山大藻GS属于GSⅠ类型。GS反应的最适pH和温度分别为8.0和25℃,Mg^(2+)离子参与的GS反应,GS活力表达最高,Cu^(2+)、K+、Zn^(2+)、Ni^(2+)、Ca^(2+)、Ce^(2+)、Mn^(2+)对GS活力均有一定的抑制作用,Fe^(2+)和Fe^(3+)则完全抑制了GS活力。

关键词：谷氨酰胺合成酶(GS) 离子交换柱分子筛

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Natural vibration and critical velocity of translating Timoshenko beam with non-homogeneous boundaries

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Applied Mathematics and Mechanics(English Edition) 2024年第9期45卷 1523-1538页

作者： Yanan LI Jieyu ding Hu ding Liqun CHEN Shanghai Institute of Applied Mathematics and Mechanics Shanghai Key Laboratory of Mechanics in Energy EngineeringShanghai Frontier Science Center of MechanoinformaticsSchool of Mechanics and Engineering ScienceShanghai UniversityShanghai 200072China School of Mathematics and Statistics Qingdao UniversityQingdao 266071Shandong ProvinceChina

In most practical engineering applications,the translating belt wraps around two fixed *** boundary conditions of the dynamic model are typically specified as simply supported or fixed *** this paper,non-homogeneous boundaries are introduced by the support *** the translating belt as the mechanical prototype,the vibration characteristics of translating Timoshenko beam models with nonhomogeneous boundaries are investigated for the first *** governing equations of Timoshenko beam are deduced by employing the generalized Hamilton's *** effects of parameters such as the radius of wheel and the length of belt on vibration characteristics including the equilibrium deformations,critical velocities,natural frequencies,and modes,are numerically calculated and *** numerical results indicate that the beam experiences deformation characterized by varying curvatures near the *** radii of the wheels play a pivotal role in determining the change in trend of the relative difference between two beam *** the results unearths that the relative difference in equilibrium deformations between the two beam models is more pronounced with smaller-sized *** the two wheels are of equal size,the critical velocities of both beam models reach their respective *** addition,the relative difference in natural frequencies between the two beam models exhibits nonlinear variation and can easily exceed 50%.Furthermore,as the axial velocities increase,the impact of non-homogeneous boundaries on modal shape of translating beam becomes more *** dealing with non-homogeneous boundaries is challenging,beam models with non-homogeneous boundaries are more sensitive to parameters,and the differences between the two types of beams undergo some interesting variations under the influence of non-homogeneous boundaries.

关键词： translating beam Timoshenko beam non-homogeneous boundary natural frequency critical velocity

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Whittle’s Index Based Sensor Scheduling for Multiprocess Systems Under DoS Attacks

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Journal of Systems Science & Complexity 2024年第1期37卷 329-350页

作者： CAI Zai ding Kemi Shenzhen Key Laboratory of Control Theory and Intelligent Systems Southern University of Science and TechnologyShenzhen 518055China.

In this paper,the authors consider how to design defensive countermeasures against DoS attacks for remote state estimation of multiprocess *** each system,a sensor will measure its state and transmits the data packets through an unreliable channel which is vulnerable to be jammed by an *** limited communication bandwidth,only a subset of sensors are allowed for data transmission,and how to select the optimal one to maximize the accuracy of remote state estimation is the focus of the proposed *** authors first formulate this problem as a Markov decision process and investigate the existence of optimal ***,the authors demonstrate the piecewise monotonicity structure of optimal *** the difficulty of obtaining an optimal policy of large-scale problems,the authors develop a suboptimal heuristic policy based on the aforementioned policy structure and Whittle’s ***,a closed form of the indices is derived in order to reduce implementation complexity of proposed scheduling policy and numerical examples are provided to illustrate the proposed developed results.

关键词： DoS attacks Kalman filter remote state estimation Whittle’s index.

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Deep structure of the Southeast Asian curved subduction system and its dynamic process

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Journal of Oceanology and Limnology 2024年第3期42卷 701-704页

作者： Weiwei ding Key Laboratory of Submarine Geoscience&Second Institute of Oceanography Ministry of Natural ResourcesHangzhou 310012China School of Oceanography Shanghai Jiao Tong UniversityShanghai 200030China

The deep structure,material circulation,and dynamic processes in the Southeast Asia have long been an elusive scientific puzzle due to the lack of systematic scientific observations and recognized theoretical *** on the deep seismic tomography using long-period natural earthquake data,in this study,the deep structure and material circulation of the curved subduction system in Southeast Asia was studied,and the dynamic processes since 100 million years ago was *** is pointed out that challenges still exist in the precise reconstruction of deep mantle structures of the study area,the influence of multi-stage subduction on deep material exchange and shallow magma activity,as well as the spatiotemporal evolution and coupling mechanism of multi-plate *** work should focus on high-resolution land-sea joint 3-D seismic tomography imaging of the curved subduction system in the Southeast Asia,combined with geochemical analysis and geodynamic modelling works.

关键词： curved subduction system deep structure material recycling dynamic process Southeast Asia

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A vertical track nonlinear energy sink

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Applied Mathematics and Mechanics(English Edition) 2024年第6期45卷 931-946页

作者： Meng LI Hu ding Shanghai Key Laboratory of Mechanics in Energy Engineering School of Mechanics and Engineering ScienceShanghai Frontier Science Center of MechanoinformaticsShanghai UniversityShanghai 200444China

Eliminating the effects of gravity and designing nonlinear energy sinks(NESs)that suppress vibration in the vertical direction is a challenging task with numerous damping *** this paper,the dynamic design of a vertical track nonlinear energy sink(VTNES)with zero linear stiffness in the vertical direction is proposed and realized for the first *** motion differential equations of the VTNES coupled with a linear oscillator(LO)are *** the strong nonlinearity considered of the VTNES,the steady-state response of the system is analyzed with the harmonic balance method(HBM),and the accuracy of the HBM is verified *** this basis,the VTNES prototype is manufactured,and its nonlinear stiffness is *** damping effect and dynamic characteristics of the VTNES are studied theoretically and *** results show that the VTNES has better damping effects when strong modulation responses(SMRs)***,even for small-amplitude vibration,the VTNES also has a good vibration suppression *** sum up,in order to suppress the vertical vibration,an NES is designed and developed,which can suppress the vertical vibration within certain ranges of the resonance frequency and the vibration intensity.

关键词： track nonlinear energy sink(TNES) vertical direction gravity nonlinear stiffness harmonic balance method(HBM) vibration control

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Capturing metal clusters at the strong-interaction region to reverse Ostwald ripening

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Science China Materials 2024年第4期67卷 1363-1364页

作者： Shuheng Tian ding Ma Beijing National Laboratory for Molecular Sciences New Cornerstone Science LaboratoryCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

High-temperature interactions induce sintering of metal nanoparticles(NPs) on heterogeneous catalyst surfaces, leading to reduced surface area and active site loss through Ostwald ripening [1]. This irreversible deactivation necessitates costly catalyst replacement, notably impacting metals like copper in hydrogenation and reforming reactions [2].

关键词： catalyst reforming irreversible

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