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Stress Echocardiography for Chronic Coronary Syndrome:Clinical Practice Guidelines(2023)

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Journal of Geriatric Cardiology 2024年第5期21卷 475-505页

作者： Peng-Fei ZHANG Jia-Wei TIAN Tian-Gang ZHU Jue-Fei WU Xiao-Ping LENG Yi WANG Meng-Meng LI Xin-Hao LI Qian-Qian WANG Xiao-Peng FENG Jia-Yan LV Li-Xue YIN Yun ZHANG Mei ZHANG chinese Society of Echocardiography,Superficial Tissue and Vascular Group of chinese Society of Ultrasound in Medicine,Ultrasound Professional Committee of chinese Medicine Education Association State Key Laboratory for Innovation and Transformation of Luobing Theory Key Laboratory of Cardiovascular Remodeling and Function ResearchChinese Ministry of EducationChinese National Health Commission and Chinese Academy of Medical SciencesDepartment of CardiologyQilu Hospital of Shandong UniversityJinanChina The second affiliated hospital of Harbin medical University HaerbinChina Peking University People's hospital BeijingChina Sichuan Province People's hospital ChengduChina Nanfang hospital Southern Medical UniversityGuangzhouChina Peking Union medical college hospital Chinese Academy of Medical SciencesBeijingChina 不详

In the context of the People's Republic of china,coronary artery disease(CAD)presents a sig-nificant clinical challenge,with over 11.3 mil-lion patients ***,the diagnos-is of CAD has predominantly relied on invasive coronary angiography.[1]However,recent advances in clinical research have revealed a notable trend:a substantial 82% of patients subjected to such invas-ive diagnostics do not necessitate interventional therapy.

关键词： coronary clinical patients

Specific CEA-producing colorectal carcinoma cell killing with recombinant adenoviral vector containing cytosine deaminase gene

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World Journal of Gastroenterology 2002年第2期8卷 270-275页

作者： Li-Zong Shen Wen-Xi Wu Qiang Ding Yi-Bing Hua,department of General surgery,The First affiliated hospital of Nanjing medical University,Nanjing,210029,Jiangsu Province,china De-Hua Xu Zhong-Cheng Zheng Xin-Yuan Liu,Shanghai Institute of Biochemistry and Cell Biology,The chinese Academy of Sciences,Shanghai,200031,china Kun Yao,department of Microbiology and Immunology,Nanjing medical University,Nanjing,210029,Jiangsu Province,china department of General surgery The First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road Nanjing 210029 Jiangsu Province China.

AIM: To kill CEA positive colorectal carcinoma cells specifically using the E coli cytosine deaminase (CD) suicide gene, a new replication-deficient recombinant adenoviral vector was constructed in which CD gene was controlled under CEA promoter and its in vitro cytotoxic effects were evaluated. METHODS: Shuttle plasmid containing CD gene and regulatory sequence of the CEA gene was constructed and recombined with the right arm of adenovirus genome DNA in 293 cell strain. Dot blotting and PCR were used to identify positive plaques. The purification of adenovirus was performed with ultra-concentration in CsCl step gradients and the titration was measured with plaque formation assay. Cytotoxic effects were assayed with MTT method, The fifty percent inhibition concentration (IC(50)) of 5-FC was calculated using a curve-fitting parameter. The human colorectal carcinoma cell line, which was CEA-producing, and the CEA-nonproducing Hela cell line were applied in cytological tests. An established recombinant adenovirus vector AdCMVCD, in which the CD gene was controlled under CMV promoter, was used as virus control. Quantitative results were expressed as the mean +/- SD of the mean. Statistical analysis was performed using ANOVA test. RESULTS: The desired recombinant adenovirus vector was named AdCEACD. The results of dot blotting and PCR showed that the recombinant adenovirus contained CEA promoter and CD gene. Virus titer was about 5.0 X 10(14)pfu/L(-1) after purification. The CEA-producing Lovo cells were sensitive to 5-FC and had the same cytotoxic effect after infection with AdCEACD and AdCMVCD (The IC(50) values of 5-FC in parent Lovo cells, Lovo cells infected with 100 M.O.I AdCEACD and Lovo cells infected with 10 M.O.I AdCMVCD were >15000, 216.5+/-38.1 and 128.8+/-25.4 micromol.L(-1), P<0.001, respectively), and the cytotoxicity of 5-FC increased accordingly when the m.o.i of adenoviruses were enhanced (The value of IC(50) of 5-FC was reduced to 27.9+/-4.2 micr

关键词： Gene Therapy Genetic Vectors Adenoviridae Animals Antimetabolites Bystander Effect Carcinoembryonic Antigen Cell Line Colorectal Neoplasms Cytosine Deaminase Flucytosine Hela Cells Humans Nucleoside Deaminases Promoter Regions (Genetics) Research Support, Non-U.S. Gov't Tumor Cells, Cultured

Inhibition of Tumor Growth in Mice by Endostatin Derived from Abdominal Transplanted Encapsulated Cells

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 278-284页

作者： Huaining TENG~(1.3) Ying ZHANG~2 Wei WANG~2 Xiaojun MA~2* Jian FEI~1* 1 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Model Organism Research Center,Shanghai Institutes for Biological Sciences,chinese Academy of Sciences,Shanghai 200031,china 2 Laboratory of Biomedical Material Engineering,Dalian Institute of Chemical Physics,chinese Academy of Sciences,Dolian 116023,china 3 department of Urology,Changzheng hospital,second Military medical University,Shanghai 200003,china Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China department of Urology Changzheng Hospital Second Military Medical UniversityShanghai 200003 China Laboratory of Biomedical Material Engineering Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023 China

Endostatin,a C-terminal fragment of collagen 18a,inhibits the growth of established tumorsand metastases in vivo by inhibiting ***,the purification procedures required for large-scale production and the attendant cost of these processes,together with the low effectiveness in clinicaltests,suggest that alternative delivery methods might be required for efficient therapeutic use of *** the present study,we transfected chinese hamster ovary(CHO)cells with a human endostatin geneexpression vector and encapsulated the CHO cells in alginate-poly-L-lysine *** release ofbiologically active endostatin was confirmed using the chicken chorioallantoic membrane *** encap-sulated endostatin-expressing CHO cells can inhibit the growth of primary tumors in a subcutaneous B 16tumor model when injected into the abdominal cavity of *** results widen the clinical applicationof the microencapsulated cell endostatin delivery system in cancer treatment.

关键词： microencapsulation endostatin CHO tumor gene therapy

Genetic polymorphism and mRNA levels of cytochrome P450ⅡE1 and glutathione S-transferase P1 in patients with alcoholic liver disease in different nationalities

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Hepatobiliary & Pancreatic Diseases International 2009年第2期8卷 162-167页

作者： Ying Liu,Xiang-Wei Meng,Ling-Yun Zhou,Pei-Yi Zhang,Xun Sun and Ping Zhang department of Gastroenterology,First affiliated hospital,Jilin University,Changchun 130021,china department of Gastroenterology,Heilongjiang Province hospital,Harbin 150036,china department of medical Molecular Biology,Harbin medical University,Harbin 150086,china department of Gastroenterology First Affiliated Hospital Jilin University Changchun 130021 China.

BACKGROUND:Alcohol abuse and dependence are major factors in the pathogenesis of alcoholic liver disease(ALD).Alcohol abuse is becoming an increasingly severe problem among the Han,Mongol,and Korean nationalities in northeast *** study aimed to investigate the relationship between ALD and the genetic polymorphism and expression levels of two enzymes,cytochrome P450ⅡE1(CYPⅡE1)and glutathione S-transferase P1(GSTP1)in patients of three ***:Peripheral blood was collected from 353 chinese patients with ALD,300 alcohol dependent patients without liver disease(alcoholic),and 360 healthy *** group included patients from the Han,Mongol and Korean ***-time polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP)were ***:Regardless of nationality,patients who carried the rare CYPⅡE1 C2 and GSTP1 Val alleles were at higher risk of *** frequency of C2 and Val in patients with ALD was respectively 50.00%and 26.98%in the Han,31.36%and 22.87%in the Mongol,and 45.87%and 22.02% in the Korean *** significant differences were seen in the frequency of either C2 or Val alleles in ALD patients among the three *** each nationality,the frequency of both C2 and Val alleles was significantly higher in ALD compared to alcoholic and healthy *** for nationality,the average mRNA levels of CYPⅡ E1 in ALD patients and healthy controls were 10.05%and 2.21%,*** average mRNA levels of GSTP1 in ALD patients and healthy controls were 0.53%and 2.12%,*** mRNA level of CYPⅡE1 was higher,and that of GSTP1 was lower in patients with ALD compared to the ***:Except for nationality,patients with ALD in this series tended to have a higher mRNA expression of CYPⅡE1 and to carry the C2 allele,and tended to have a lower mRNA expression of GSTP1 and to carry the Val *** is a causal relationship between the polymorphic alleles,which lea

关键词： alcoholic liver disease alcoholic genetic polymorphism mRNA expression

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灰毡毛忍冬的生药学研究

时珍国医国药 2005年第11期16卷 1076-1077页

作者：税丕先刘家奇孙琴庄元春泸州医学院药学院四川泸州646000 四川省泸州市中医医院 646000

目的:为灰毡毛忍冬的鉴别,开发利用提供依据。方法:采用来源、性状、显微、理化对其鉴别。结果:首次系统阐明了灰毡毛忍冬的生药学理论。结论:为制订灰毡毛忍冬质量标准提供依据。

关键词：灰毡毛忍冬来源性状显微理化鉴别

Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin

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Acta Pharmacologica Sinica 2006年第10期27卷 1367-1374页

作者： Hal-tao ZHANG~(2.4) Jun WU~3 Hai-feng ZHANG~2 Qi-feng ZHU ~2Institute of Biochemistry.and Molecular Biology,Guangdong medical college,Zhanjiang 524023,china ~3affiliated hospital of Guangdong medical college,Zhanjiang 524023,china Institute of Biochemistry and Molecular Biology Guangdong Medical College Zhanjiang China affiliated hospital of Guangdong medical college Zhanjiang China

Aim:To investigate the role of intercellular potassium in tachyplesin-induced HL-60cells ***:The concentration of intercellular potassium,cell vol-ume and mitochondrial membrane potential were examined by flow ***:The concentration of intercellular potassium reduced in a time-depen-dent manner in tachyplesin-treated HL-60 *** addition,the loss of mitochon-drial membrane potential was tightly coupled with the shrinkage of cell *** caspase inhibitors protected against DNA degradation but did not pre-vent the loss of HL-60 cell viability induced by ***2+,which was akind of blocker of volume-regulatory K+channels,increased the viability oftachyplesin-treated HL-60 cells and maintained mitochondrial membrane potentialand cell ***:Efflux of K+was an important reason for apoptosisin tachyplesin-treated HL-60 *** of K+affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation.

关键词： horseshoe crab tachyplesin intracellular potassium mitochondrial membrane potential HL-60 cell

Two-photon spectroscopic behaviors and photodynamic effect on the BEL-7402 cancer cells of the new chlorophyll photosensitizer

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Science china Chemistry 2008年第6期51卷 529-538页

作者： ZHAO PeiDe1,2,CHEN Ping1,TANG GuoQing1,LIU JinWei2,LIN Lie1,GUO Peng1,YU Qing2, YAO JianZhong3,MA DongMing4,ZHANG GuiLan1 & CHEN WenJu1 1 Key Laboratory of Optoelectronic Information Science and Technology,Ministry of Education,Institute of Modern Optics,Nankai University,Tianjin 300071,china 2 department of Physics,Hebei University of Technology,Tianjin 300130,china 3 college of Pharmacy,second Military medical University,Shanghai 200433,china 4 Tianjin college of the traditional chinese Medicine,Tianjin 300193,china Key Laboratory of Optoelectronic Information Science and Technology Ministry of Education Institute of Modern Optics Nankai University Tianjin China department of Physics Hebei University of Technology Tianjin China college of Pharmacy Second Military Medical University Shanghai China Tianjin college of the traditional chinese Medicine Tianjin China

The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ'_2 ≈ 31.5x10^(-50)cm^4·s·photon^(-1). The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.

关键词： photosensitizer chlorophyll derivate two-photon fluorescence two-photon absorption cross-section TPPDT BEL-7402 cell

勃起功能障碍和早泄共病中西医结合诊治中国专家共识

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中国实验方剂学杂志 2024年第7期30卷 147-153页

作者：中国性学会中医性学分会专家共识编写组王权胜不详广西中医药大学第一附属医院

目前,国内对于勃起功能障碍(ED)/早泄(PE)共病的中西医结合诊疗和管理缺乏共识,为制订ED/PE共病中西医结合临床诊疗策略,根据近年来国内外发布的ED/PE共病指南并结合全国多家泌尿外科、男科研究单位的临床实践,中国性学会中医性学分会... 详细信息

目前,国内对于勃起功能障碍(ED)/早泄(PE)共病的中西医结合诊疗和管理缺乏共识,为制订ED/PE共病中西医结合临床诊疗策略,根据近年来国内外发布的ED/PE共病指南并结合全国多家泌尿外科、男科研究单位的临床实践,中国性学会中医性学分会组织相关临床专家根据现有的循证医学证据,经共识起草、学会审核、专家函审访谈、审稿会的程序充分讨论形成本共识。该共识针对中西医结合的诊疗定位和价值,基于医生临床经验、研究证据、患者意愿和生活环境因素,综合评估做出ED/PE共病的中西医结合诊治规范,为更多患者在诊疗进程中获益。中医诊断注重望诊、闻诊、问诊、切诊四诊信息的收集,并据此进行《伤寒论》六经辨证、卫气营血辨证、脏腑辨证。基于中西医协同方式,发挥中医药整体调节的优势,实施个性化中西医结合治疗。中药内服治疗根据《中医外科学》第四章外科疾病的辨证第二节阴阳辨证。《类经·阴阳类》“人之疾病……必有所本,或本于阴,或本于阳,病变虽多,其本则一。”《黄帝内经·素问·阴阳应象大论》“善诊者,察色按脉,先别阴阳。”该共识依据“八纲辨证,首辨阴阳”分为阳证、阴证两类。此外,对ED/PE共病的证型证候,基于异病同治有应用柴胡桂枝汤、温经汤、补中益气汤治疗本病,可以参考。

关键词：勃起功能障碍(ED) 早泄(PE) ED/PE共病中西医结合共识

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A Study of the Law of Herbal Administration in Treating Lung-distension by TCM Physicians through History Using Cluster Analysis

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Journal of traditional chinese Medicine 2011年第4期31卷 303-307页

作者：高振李风森哈木拉提·吾甫尔 traditional chinese Medicine hospital affiliated with Xinjiang medical University & National Clinical Research Base of traditional chinese Medicine Xin Jiang medical University

Objective: To review the herbal drugs used most often for treating lung-distension by determining those used by physicians throughout history; to study their property, taste, and channel tropism; and to explore their compatibility. Methods: Historical prescriptions for treating lung-distension were collected and sorted. Property, taste and channel tropism were determined, and the law of herbal administration was determined by cluster analysis. Results: One hundred and ninety five prescriptions were found, involving 166 herbal drugs, with a total appearance frequency of 1296 drugs. The herbs involved 8 properties (total appearance frequency, 1296), 7 forms of taste (total appearance frequency, 1991) and involved all 12 regular channels (total appearance frequency, 3382). Sixteen herbal drugs were used most often and formed 4 cluster prescriptions: C1: Xing Ren (Semen Armeniacae Amarum), Gan Cao (Radix Glycyrrhizae), and Ban Xia (Rhizoma Pinelliae); C2: Gui Zhi (Ramulus Cinnamomi), Wu Wei Zi (Fructus Schisandrae), and Sheng Jiang (Rhizoma Zingiberis Recens); C3: Da Zao (Fructus Jujubae), Ma Huang (Herba Ephedrae), and Shi Gao (Gypsum Fibrosum); and C4: Chen Pi (Pericarpium Citri Reticulatae), Jie Geng (Radix Platycodi), Fu Ling (Poria), Sang Bai Pi (Cortex Mori), Xi Xin (Herba Asari), Gan Jiang (Rhizoma Zingiberis), and Bai Shao (Radix Paeoniae Alba). Conclusion: In treating lung-distension, phlegm retention has been traditionally considered the underlying pathology, emphasizing regulation of the lung and spleen as key and stressing patient nourishment and mental improvement. Prescriptions for lung-distension should be made with reference to the property, taste, channel tropism, and effectiveness of the chosen herbal drugs.

关键词： ancient medical literature cluster lung-distension property taste and channel tropism the law of herbal administration