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Deguelin regulates nuclear pore complex proteins Nup98 and Nup88 in U937 cells in vitro1

Acta Pharmacologica Sinica 2005年第10期26卷 1265-1273页

作者： Hong-li LIU~2. Yan CHEN~(2.3), Guo-hui CUI~2. Qiu-ling WU~2. Jing HE~2. Wei-hua CHEN~2. Jian-feng ZHOU~4 ~2.department of Hematology, Union hospital, Tongji {2. College, Huazhong university of science and Technology, Wuhan 43002. ~4 department of Hematology, Tongji hospital, Tongji {2. College, Huazhong university of science and Technology, Wuhan 430030, China department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim: To investigate the anticancer effects and the molecular mechanisms of deguelin on human U937 leukemia cells, and to explore the underlying mechanism regulating nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro. Methods: The effects of deguelin on the growth of U937 cells were studied by MTT assay. The effect of deguelin on the cell cycle of U937 cells was studied by using a propidium iodide method. The localization of the nuclear pore complex proteins Nup98 and Nup88 was investigated by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 and Nup88 in U937 cells was investigated by using flow cytometry and Western blot. Results: The prolif eration of U937 cells was inhibited in the deguelin-treated group, with a 2.-h IC50value of 2..61 nmol/L and a 36-h IC50value of 17.07 nmol/L. U937 cells treated with deguelin had reduced percentages of cells in the G0/G1phase, whereas cells accumulated in the S and G2.M phases. Nup88 and Nup98 were found on both the nuclar and cytoplasmic sides of the U937 cells by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 was upregulated and that of the Nup88 protein was downregulated in U937 cells treated with deguelin. Conclusion: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle such that cells are arrested at the S and G2.M phases, so that the proportion of cells in the G0/G1phase decreases. The antitumor effects of deguelin are related to upregulating the expression of Nup98 and downregulating the expression of Nup88 protein in U937 cells.

关键词： deguelin U937 cells nucleoporin Nup98 Nup88

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Chinese consensus on the diagnosis and treatment of immunoglobulin light-chain cardiac amyloidosis

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Chinese medical Journal 2024年第2期137卷 127-129页

作者： Kaini Shen Zhuang Tian Yajuan Gao Yining Wang Li Huo Jian Li Yun Zhang Shuyang Zhang On behalf of Chinese Society of Rare Diseases and Rare Diseases Branch of China Research hospital Association department of Hematology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Cardiology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Clinical Medicine Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Radiology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Nuclear Medicine Beijing Key Laboratory of Molecular Targeted Diagnosis and Therapy in Nuclear MedicineState Key Laboratory of Complex Severe and Rare DiseasesCenter for Rare Diseases ResearchPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Cardiology The Key Laboratory of Cardiovascular Remodeling and Function ResearchChinese Ministry of EducationChinese National Health Commission and Chinese Academy of Medical SciencesThe State and Shandong Province Joint Key Laboratory of Translational Cardiovascular MedicineQilu HospitalCheeloo College of MedicineShandong UniversityJinanShandong 250012China Cardiovascular Disease Research Center of Shandong First medical university Central Hospital Affiliated to Shandong First Medical UniversityJinanShandong 250013China 不详

Epidemiology of Amyloid Light-Chain Cardiac Amyloidosis(AL-CA)The annual incidence of amyloid light-chain(AL)amyloidosis is 3-5/million,and the incidence in men is slightly higher than that in ***,70%of patients with ... 详细信息

关键词： amyloid cardiac diagnosis

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BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

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Biomedical and Environmental sciences 2011年第2期24卷 89-93页

作者： HU Ying CHEN ErJun MU Yue LI JinLu CHEN RenJi beijing Stomatological hospital Capital Medical University Beijing 100050 China beijing Tongrenhospital Capital Medical University Beijing 100730 China

Objective Convincing evidence suggests a link between increased risk of nonsyndromic cleft lip with or without cleft palate （NSCL/P） and low intake of folic acid by the mother during pregnancy. The present study was designed to explore if genetic variation in the betaine‐homocysteine methyltransferase （BHMT） gene contributes to NSCL/P. Methods DNA was obtained from 166 individuals with NSCL/P and 2.5 healthy subjects. Three known single nucleotide polymorphisms （SNPs） present in the BHMT gene （rs651852. rs3797546, and rs3733890） were investigated by real‐time PCR‐based TaqMan genotyping. Results Neither allelic nor genotypic association was found between NSCL/P and SNPs rs651852.and rs3733890. SNP rs3797546 did not show allelic association with NSCL/P; however, a higher proportion of NSCL/P patients carry the CC genotype compared with the TT＋CT genotype （P=0.02., OR=2.10, 95% CI=1.11‐3.95）. Conclusion Our study suggests that polymorphism rs3797546 in the BHMT gene may confer genetic risk of NSCL/P in a recessive manner.

关键词： Nonsyndromic cleft lip with or without palate Homocysteine Folate Polymorphisms

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细胞免疫表型分析诊断骨髓增生异常综合征临床意义

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中国实用内科杂志 2010年第5期30卷 401-403页

作者：徐娟首都医科大学宣武医院血液科北京100053

骨髓增生异常综合征(MDS)是克隆性造血干细胞疾病,其基本病理特征是无效造血,并表现为骨髓细胞病态造血和细胞表面标记的混杂表达。对MDS患者进行细胞免疫表型检测可以分析原始细胞群、粒细胞群和单核细胞群的表达异常,有助于MDS的诊断... 详细信息

骨髓增生异常综合征(MDS)是克隆性造血干细胞疾病,其基本病理特征是无效造血,并表现为骨髓细胞病态造血和细胞表面标记的混杂表达。对MDS患者进行细胞免疫表型检测可以分析原始细胞群、粒细胞群和单核细胞群的表达异常,有助于MDS的诊断。由于对MDS细胞免疫表型分析是一个非常复杂的过程,规范与统一标准、使检测结果具有可比性和可信性是关键问题。

关键词：骨髓增生异常综合征细胞免疫表型分析

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Application of Transcranial Doppler in Subarachnoid Hem orrhage

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Journal of Huazhong university of science and Technology({2. sciences) 1999年第3期19卷 230-232页

作者： JIANG Yaping , SHI Qijia , GAO Qiong department of neurology, Tongji hospital, Tongji {2. university, Wuhan 430030 department of neurology Tongji Hospital Tongji Medical University Wuhan

Transcranial Doppler (TCD) was dynamically performed on 2. patients with subarachnoid hemorrhage (SAH) before and after treatment. In the first 4 days of clinical course, 3 cases demonstrated elevated intracranial pressure (ICP), 6 cases showed stenosis of blood vessels, and 16 cases gave normal findings. At 7-14 days of clinical course, 2. cases showed vascular spasm or narrowing, 4 cases imparted normal findings and 1 patient died of elevated ICP. After the third TCD performed on the 2.th day of the clinical course, 5 cases showed mean blood flow velocities (Vm) below normal values, indicating poor cerebral perfusion, 9 cases still had Vm above normal levels, 10 cases yielded completely normal findings. In conclusion, TCD conducted at different phases of SAH can not only demonstrate the functional and pathological status of cerebral blood vessels following SAH, but also reflect the outcome of clinical features. It is very helpful in the evaluation of the causes and the prognosis of SAH.

关键词： SAH TCD diagnosis prognosis

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Establishment of liver specific glucokinase gene knockout mice: a new animal model for screening anti-diabetic drugs

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Acta Pharmacologica Sinica 2004年第12期25卷 93-99页

作者： Ya-li ZHANG, Xiao-hong TAN2. Mei-fang XIAO, Hui LI, Yi-qing Mao, Xiao YANG2. Huan-ran TAN3 department of Pharmacology, School of Basic medical {2., Peking university Health {2. Center, beijing 100083 2.nstitute of Biotechnology Academy ofMilitary medical {2., beijing 100071, China department of Pharmacology School of Basic Medical Science Peking University Health Science Center 北京 100083 Institute of Biotechnology Academy of Military medical science 北京 100071

AIM: To characterize the liver-specific role of glucokinase in maintaining glucose homeostasis and to create an animal model for diabetes. METHODS: We performed hepatocyte-specific gene knockout of glucokinase in mice using Cre-loxP gene targeting strategy. First, two directly repeated loxP sequences were inserted to flank the exon 9 and exon 10 of glucokinase in genomic DNA. To achieve this, linearized targeting vector was electroporated into ES cells. Then G418- and Gancyclovir-double-resistant clones were picked and screened by PCR analysis and the positives identified by PCR were confirmed by Southern blot. A targeted clone was selected for microinjection into C57BL/6J blastocysts and implanted into pseudopregnant FVB recipient. Chimeric mice and their offspring were analyzed by Southern blot. Then by intercrossing the Alb-Cre transgenic mice with mice containing a conditional gk allele, we obtained mice with liver-specific glucokinase gene knockout. RESULTS: Among 161 double resistant clones 4 were positive to PCR and Southern blot and only one was used for further experiments. Eventually we generated the liver specific glucokinase knockout mice. These mice showed increased glucose level with age and at the age of 6 weeks fasting blood glucose level was significantly higher than control and they also displayed impaired glucose tolerance. CONCLUSION: Our studies indicate that hepatic glucokinase plays an important role in glucose homeostasis and its deficiencies contribute to the development of diabetes. The liver glucokinase knock- out mouse is an ideal animal model for MODY2. and it also can be applied for screening anti-diabetic drugs.

关键词： animal disease model non insulin dependent diabetes mellitus type 2.diabetes mellitus glucokinase knockout mice

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Effects of the CNTF-collagen gel-controlled delivery system on rat neural stem/progenitor cells behavior

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science China(Life sciences) 2010年第4期53卷 504-510页

作者： YANG ZhaoYang1, QIAO Hui2.& LI XiaoGuang1,3 1School of Biological science and medical Engineering, Beihang {2., beijing 100191, China 2.ublic Heath School, Ningxia medical {2., Yinchuan 750004, China 3beijing Institute for Neuroscience, capital medical {2., beijing 100069, China School of Biological science and medical Engineering Beihang University Beijing China Public Heath School Ningxia Medical University Yinchuan China beijing Institute for Neuroscience Capital Medical University Beijing China

The injury of central nervous system (CNS) usually causes the cavity formation. Although transplantation of neural stem/precursor cells (NSPCs) into the lesioned area of CNS has been shown to be implicated in the functional restoration, the therapeutic result is limited by the poor survival of NSPCs as well as their insufficient proliferation and differentiation abilities. Type-1 collagen is considered as a candidate scaffold or drug delivery system to overcome the aforementioned obstacle. This study observed the effects of the CNTF (ciliary neurotrophic factor)-collagen gel-controlled delivery system and daily addition of soluble-form CNTF on the NSPC survival, migration, proliferation and differentiation. The results showed that, within 12.h of the initial co-culture, CNTF was released in a burst pattern, then the CNTF-collagen gel-controlled delivery system stably released CNTF for up to 12.d. The cell viability test, together with immunohistochemistry, RT-PCR and Western blotting, showed that the CNTF-collagen gel-controlled delivery system supported the NSPCs seeded on the surface of collagen gel survival and facilitated their migration and proliferation. The daily addition of soluble-form CNTF to the medium had similar effects to the CNTF-collagen gel-controlled delivery system, but large quantities of soluble-form CNTF were consumed during the entire process. Taken together, the CNTF-collagen gel-controlled delivery system not only provides a physical scaffold for the transplanted NSPCs to adhere and migrate, but also facilitates the NSPC survival, growth and proliferation, simultaneously reducing the consumption of the expensive growth factors. This system may be used to enhance the microenvironment in the lesioned area of CNS.

关键词： spinal cord neural stem/progenitor cells control release type-1 collagen CNTF

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A novel endorectal RF coil for high resolution MRI and spectroscopy of the prostate

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Progress in Natural science:Materials International 2005年第5期15卷 472-476页

作者： ZHANG Hongjie 1,2. SONG Xiaoyu1, WANG Xiaoying3, XU Pingfang1 and BAO Shanglian1*(1. beijing Key Laboratory of medical Physics & Engineering, Peking {2., beijing 100871, China 2. Navy General hospital, beijing 100037, China 3. Peking {2. First hospital, beijing 100034, China) beijing Key Lab. of Med. Phys. and Eng. Peking Univ. Beijing 100871 China Navy General hospital Beijing 100037 China Peking Univ. First hospital Beijing 100034 China

The diagnosis of prostate diseases by magnetic resonance imaging (MRI) in vivo is sometimes difficult for the lower signal to noise ratio (SNR). To increase the SNR of the prostate image, we designed a RF coil that can be inserted into rectum and was named endorectal coil. The properties of RF coil were evaluated using a network analyzer. Moreover the images and spectroscopy of a special phantom were acquired and the results were compared to those of the commercial TORSO coil (G.E. medical Systems, USA). Our coil gave a significantly higher SNR at the region of interest (ROI). The achieved high local SNR and resulting high spatial resolution would add more anatomic and biochemical information to the diagnosis of prostate diseases.

关键词： MRI RF coil endorectal coil.

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Evaluation of frequency-lowering algorithms for intelligibility of Chinese speech in hearing-aid users

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Progress in Natural science:Materials International 2009年第6期19卷 741-749页

作者： Xianbo Xiao a, Hui Zhang a, Guangshu Hua, Chunhong Liu b, Jia Liu c a Biomedical Engineering, Tsinghua university, Room 2.9, medical Tech. Bld., beijing 100084, China b department of Otolaryngology, xuanwu hospital, beijing 100053, China c Bo Na Feng medical Instrument Co., Ltd., beijing 10002., China Biomedical Engineering Tsinghua University Room 209 Medical Tech. Bld. Beijing 100084 China department of Otolaryngology Xuanwu Hospital Beijing 100053 China Bo Na Feng medical Instrument Co. Ltd. Beijing 100028 China

Despite the attention being given to and the knowledge of the benefits of evaluating frequency-lowering algorithms for hearing-impaired people, the causality between these algorithms and their benefits is still not clear. This is aggravated by the fact that comparative research on the methodologies and skills required for fitting an appropriate algorithm to individual patients is lacking. Against this back-drop, the current study has attempted to make progress in this area. In this experiment, six experienced traditional hearing-aid users with severe impairments in the high frequencies were fitted with two different frequency-lowering methods, and weekly hearing tests were conducted to track the benefits of such methods. After the experiment, five of the listeners accepted the frequency-lowering algorithms. Both methods showed superior results when compared with the listeners’ own hearing aids in most of the tests, and the segmented compression algorithm was indicated to have better "anti-noise" quality and speech intelligibility improvement capabilities. A preference for the application of proportional compression to unvoiced speech was also found in this algorithm. Unlike in previous studies, all speech materials here are recorded in Chinese. Therefore, the results could also be used to evaluate the benefits of frequency-lowering to the intelligibility of Chinese speech for patients. As a result, an improvement was found, especially in the recognition of consonants. Moreover, no adverse effect was found in intonation recognition.

关键词： Hearing aid Frequency-lowering Speech intelligibility

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CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation

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Acta Pharmacologica Sinica 2007年第1期28卷 66-72页

作者： Zhen-zhong ZHENG Zheng-xiang LIU~2.department of Cardiology,Tongji hospital,Tongji {2. College,Huazhong university of science and Technology,Wuhan 430030,China department of Cardiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effects of CD 151 on the activity of endothelial NO syn-thase(eNOS),and ECV304 migration,proliferation and tube ***:pAAV-CD 151 and pAAV-anti-CD 151 were constructed and used to transientlytransfect ECV304 mediated with Lipofectamine *** transfection,the ex-pression of CD 151 was measured by Western *** migration assay wasperformed using Boyden transwell;proliferation assay was evaluated using the 3-[4,5-dimethylthiazol-2.yl]-2.5,diphenyltetrazolium bromide(MTT)method,andtube formation test was examined on matrigel,eNOS activity was assayed by L-[~3H]citrulline production from L-[~3H]*** involvement of eNOS was ex-plored using an eNOS inhibitor(L-NAME)and the effects in the process ***:CD 151 promotes cell migration,proliferation and tube *** addition,CD151 increases eNOS ***,cell migration,prolifera-tion and tube formation induced by CD 151 are inhibited when L-NAME is used,which indicates that there is an involvement of eNOS in CD151-induced cellmigration,cell proliferation and tube ***:CD 151 promotesECV304 migration,proliferation and tube *** mechanism is that CD151increases eNOS *** result also suggests that eNOS is involved in theangiogenic effects of CD151.

关键词： CDI51 endothelial NO synthase(eNOS) cell migration cell proliferation tube formation

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