CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation
CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation作者机构：Department of Cardiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2007年第28卷第1期
页 面：66-72页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 10[医学]
基 金：Project supported by a grant from the National Natural Science Foundation of China(No 30570728) the Science Research Foundation of the Health Department of Hubei Province(No JX2A04)
主 题：CDI51 endothelial NO synthase(eNOS) cell migration cell proliferation tube formation
摘 要：Aim:To investigate the effects of CD 151 on the activity of endothelial NO syn-thase(eNOS),and ECV304 migration,proliferation and tube ***:pAAV-CD 151 and pAAV-anti-CD 151 were constructed and used to transientlytransfect ECV304 mediated with Lipofectamine *** transfection,the ex-pression of CD 151 was measured by Western *** migration assay wasperformed using Boyden transwell;proliferation assay was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5,diphenyltetrazolium bromide(MTT)method,andtube formation test was examined on matrigel,eNOS activity was assayed by L-[~3H]citrulline production from L-[~3H]*** involvement of eNOS was ex-plored using an eNOS inhibitor(L-NAME)and the effects in the process ***:CD 151 promotes cell migration,proliferation and tube *** addition,CD151 increases eNOS ***,cell migration,prolifera-tion and tube formation induced by CD 151 are inhibited when L-NAME is used,which indicates that there is an involvement of eNOS in CD151-induced cellmigration,cell proliferation and tube ***:CD 151 promotesECV304 migration,proliferation and tube *** mechanism is that CD151increases eNOS *** result also suggests that eNOS is involved in theangiogenic effects of CD151.
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