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Autophagy-targeting modulation to promote peripheral nerve regeneration

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Neural Regeneration Research 2025年第7期20卷 1864-1882页

作者： Yan Chen Hongxia deng Nannan Zhang Department of Obstetrics and Gynecology West China Second HospitalSichuan UniversityChengduSichuan ProvinceChina Key Laboratory of Birth Defects and Women and Children’s Diseases Ministry of EducationSichuan UniversityChengduSichuan ProvinceChina Laboratory of Reproductive Endocrinology and Reproductive Regulation Sichuan UniversityChengduSichuan ProvinceChina National Center for Birth Defect Monitoring West China Second University HospitalSichuan UniversityChengduSichuan ProvinceChina

Nerve regeneration following traumatic peripheral nerve injuries and neuropathies is a complex process modulated by diverse factors and intricate molecular *** studies have focused on factors that stimulate axonal outgrowth and myelin ***,recent studies have highlighted the pivotal role of autophagy in peripheral nerve regeneration,particularly in the context of traumatic ***,autophagy-targeting modulation has emerged as a promising therapeutic approach to enhancing peripheral nerve *** current understanding suggests that activating autophagy facilitates the rapid clearance of damaged axons and myelin sheaths,thereby enhancing neuronal survival and mitigating injury-induced oxidative stress and *** actions collectively contribute to creating a favorable microenvironment for structural and functional nerve regeneration.A range of autophagyinducing drugs and interventions have demonstrated beneficial effects in alleviating peripheral neuropathy and promoting nerve regeneration in preclinical models of traumatic peripheral nerve *** review delves into the regulation of autophagy in cell types involved in peripheral nerve regeneration,summarizing the potential drugs and interventions that can be harnessed to promote this *** hope that our review will offer novel insights and perspectives on the exploitation of autophagy pathways in the treatment of peripheral nerve injuries and neuropathies.

关键词： autophagy autophagy related genes Charcot–Marie–Tooth diseases diabetic peripheral neuropathy metformin myelination peripheral nerve injury Schwann cells sciatic nerve Wallerian degeneration

High-dose dexamethasone regulates microglial polarization via the GR/JAK1/STAT3 signaling pathway after traumatic brain injury

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Neural Regeneration Research 2025年第9期20卷 2611-2623页

作者： Mengshi Yang Miao Bai Yuan Zhuang Shenghua Lu Qianqian Ge Hao Li Yu deng Hongbin Wu Xiaojian Xu Fei Niu Xinlong dong Bin Zhang Baiyun Liu Beijing Tiantan Hospital Capital Medical UniversityBeijingChina Department of Neurology The First Hospital of Tsinghua UniversityBeijingChina Department of Neurotrauma and Beijing Key Laboratory of Central Nervous System Injury Beijing Neurosurgical InstituteCapital Medical UniversityBeijingChina Department of Critical Care Medicine Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Center for Nerve Injury and Repair Beijing Institute of Brain DisordersChina National Clinical Research Center for Neurological DiseasesBeijingChina

Although microglial polarization and neuroinflammation are crucial cellular responses after traumatic brain injury,the fundamental regulatory and functional mechanisms remain insufficiently *** potent anti-inflammato ry agents,the use of glucoco rticoids in traumatic brain injury is still controversial,and their regulatory effects on microglial polarization are not yet *** the present study,we sought to determine whether exacerbation of traumatic brain injury caused by high-dose dexamethasone is related to its regulatory effects on microglial polarization and its mechanisms of *** vitro cultured BV2 cells and primary microglia and a controlled cortical impact mouse model were used to investigate the effects of dexamethasone on microglial ***,dexamethasone,RU486(a glucocorticoid receptor antagonist),and ruxolitinib(a Janus kinase 1 antagonist)were ***-sequencing data obtained from a C57BL/6 mouse model of traumatic brain injury were used to identify potential targets of *** Morris water maze,quantitative reverse transcription-polymerase chain reaction,western blotting,immunofluorescence and confocal microscopy analysis,and TUNEL,Nissl,and Golgi staining were performed to investigate our ***-throughput sequencing results showed that arginase 1,a marker of M2 microglia,was significantly downregulated in the dexamethasone group compared with the traumatic brain injury group at3 days post-traumatic brain *** dexamethasone inhibited M1 and M2 microglia,with a more pronounced inhibitory effect on M2microglia in vitro and in *** receptor plays an indispensable role in microglial polarization after dexamethasone treatment following traumatic brain ***,glucocorticoid receptor activation increased the number of apoptotic cells and neuronal death,and also decreased the density of dendritic spines.A possible downstream receptor signaling mechanism is th

关键词： apoptosis BV2 microglia dexamethasone glucocorticoid receptor glucocorticoids innate immune system microglial polarization neuroinflammation primary microglia traumatic brain injury

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京沪高铁桩基沉降时间曲线反分析及预测

长江科学院院报 2012年第4期29卷 57-63页

作者：邓东平李亮赵炼恒邹金锋中南大学土木工程学院长沙410075

在京沪高铁项目中,针对深厚软土地层中的桩基工程,使用FLAC3D建立的桩基模型进行计算。同时,利用桩基静载试验所得的沉降-时间曲线,在考虑土体存在蠕变效应时,结合位移反分析方法,对桩基土体参数和蠕变参数进行反演。在得到反演参数后,... 详细信息

在京沪高铁项目中,针对深厚软土地层中的桩基工程,使用FLAC3D建立的桩基模型进行计算。同时,利用桩基静载试验所得的沉降-时间曲线,在考虑土体存在蠕变效应时,结合位移反分析方法,对桩基土体参数和蠕变参数进行反演。在得到反演参数后,对桩基在长期荷载作用下的沉降进行预测。研究表明:①数值反演结果与实测结果比较相符;②使用反演参数对桩基长期荷载作用下的沉降进行预测,得出未注浆桩和注浆桩在长期荷载作用下的前期沉降量大,且基本达到最大沉降量,后期没有出现明显的蠕变效应。因此,建议可不需要通过注浆来对桩基的蠕变效应进行改善,且当在工期较为紧张时,可减少桩基静置时间。

关键词：桩基 FLAC3D 沉降蠕变反分析预测

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水位升降对顺层岩坡稳定性影响的上限分析方法

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铁道科学与工程学报 2012年第3期9卷 51-57页

作者：赵志刚赵炼恒李亮邓东平中铁七局集团有限公司河南郑州450016 中南大学土木建筑学院湖南长沙410075

在构造顺层岩石边坡的简单直线破坏模式基础上,将强度折减技术与极限分析上限定理结合,推导边坡内外水位升降影响条件下的顺层边坡稳定性安全系数的目标表达式,并采用序列二次规划迭代法进行求解。通过算例计算,分析水位变化参数对顺层... 详细信息

在构造顺层岩石边坡的简单直线破坏模式基础上,将强度折减技术与极限分析上限定理结合,推导边坡内外水位升降影响条件下的顺层边坡稳定性安全系数的目标表达式,并采用序列二次规划迭代法进行求解。通过算例计算,分析水位变化参数对顺层岩石边坡坡体安全系数和潜在破裂面的影响。研究结果表明:边坡内外水位升降对边坡安全系数和破坏范围存在不可忽视的影响,但当岩层倾角处于一定范围时,岩层倾角对边坡安全系数和破坏范围的控制程度更强。

关键词：顺层岩石边坡稳定性分析水位升降安全系数强度折减技术上限法

High-resolution imaging through dynamic scattering media: Iterative optimization based on polarimetric characteristics

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Science China Technological Sciences 2025年第1期 82-84页

作者： Meng XIANG Kai deng Tianyu WANG Jinpeng LIU Xue dong Teli XI Fei LIU Xiaopeng SHAO School of Optoelectronic Engineering Xidian University Xi'an Key Laboratory of Computational Imaging National Key Laboratory of Infrared Detection Technologies

High-resolution imaging through randomly dynamic scattered fields and highly scattered walls is an extensively sought-after capability with potential applications in various fields such as underwater imaging, biomedical imaging, and seeing through fog. Numerous methods have been proposed to unscramble object information from degraded scattered images, resulting in considerable improvements in image contrast in degraded scenarios [1].

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Selective enrichment of hepatocytes from mouse embryonic stem cells with a culture system containing cholestatic serum

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Acta Pharmacologica Sinica 2007年第12期28卷 1931-1937页

作者： Jun MIN~(2,3),Chang-zhen SHANG~2,Ya-jin CHEN,Lei ZHANG,Lu LIU,Xiao-geng deng,Mei YANG,dong-ping CHEN,JunCAO,Er-wei SONG,Ji-sheng CHENDepartment of Hepatobiliary Surgery,The Second Affiliated Hospital of Sun Yat-sen University,Guangzhou 510120,China Department of Hepatobiliary Surgery The Second Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510120China

Aim:There is increasing evidence indicating that embryonic stem (ES) cells arecapable of differentiating into hepatocyte-like cells in ***,it is neces-sary to improve the differentiation efficiency so as to promote the ***,we report an efficient culture system to support hepatocytedifferentiation from ES cells by utilizing cholestatic ***:One weekafter the induction of E14 mouse ES cells into hepatocytes with sodium butyrate,cholestatic serum was added into the culture system at various concentrationsand hepatocyte-like cells were induced to *** morphological andphenotypic markers of hepatocytes were characterized using light microscopy,immunocytochemistry,and RT-PCR,*** function of glycogen stor-age of the differentiated cells was detected by Periodic acid-Schiff (PAS) reaction,and the ratio of hepatic differentiation was determined by counting the albuminand PAS-positive ***:In the presence of conditional selective mediumcontaining cholestatic serum,numerous epithelial cells resembling hepatocyteswere *** RT-PCR analysis showed that undifferentiated ES cells didnot express any hepatic-specific markers;however,in the presence of sodiumbutyrate and conditional selective medium containing cholestatic serum,hepaticdifferentiation markers were *** staining showed thatthose ES-derived hepatocytes were α-fetoprotein,albumin,and cytokeratin 18positive,with the ability of storing *** determination of the hepaticdifferentiation ratio showed that the application of cholestatic serum efficientlyenriched ES-derived hepatocyte-like cells by inducing lineage differentiation andenhancing lineage ***:The conditional selective mediumcontaining cholestatic serum is optimal to selectively enrich hepatocyte-like cellsfrom mixed differentiated ES cells,which may provide a novel method to improvethe hepatic differentiation ratio of ES cells.

关键词： embryonic stem cells sodium butyrate hepatocyte cholestatic serum differentiation

TRMT6 and TRMT61A facilitated acute pancreatitis severity via regulation of neutrophil function

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Science China Life Sciences 2025年

作者： Yuan dong Li Su Sin Man Lam Cen Yan Yalan deng Guotao Lu Jinkui Yang Guanghou Shui Yingmei Feng Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical University Department of Science and Development Beijing Youan HospitalCapital Medical University Laboratory for Clinical Medicine Capital Medical University Neuroscience Research Institute Peking University Center of Medical and Health AnalysisPeking University State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences Pancreatic Center Department of GastroenterologyAffiliated Hospital of Yangzhou UniversityYangzhou University Beijing Key Laboratory of Diabetes Research and Care Department of EndocrinologyBeijing Tongren HospitalCapital Medical University

Dear Editor,Type 2 diabetes mellitus (T2DM) has been identified as a risk factor for increased severity in acute pancreatitis (AP)(Mikóet al,2018).However,to date,no studies have simultaneously included healthy individuals,T2DM patients,and AP patients to investigate which diabetes-associated metabolites are linked to AP severity.

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Six-fold swelling expansion microscopy assisted cell painting for high-content phenotypic screening

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Optoelectronics Letters 2025年

作者： Junfang XIE Yipeng dong Jin WANG Zhichao deng Jianguo TIAN Qing YE Key Laboratory of Weak-Light Nonlinear Photonics， Ministry of Education， School of Physics and TEDA Applied Physics， Nankai University

Imaging-based phenotypic screening uses cellular phenotypes to describe the drug performance， which generally focuses on single cellular feature， lacking of a comprehensive characterization. Here， we propose a high content phenotypic screening method based on expansion microscopy (ExM) assisted Cell Painting， which enables multi-channel imaging with approximately 50 nm resolution. As a demonstration， we applied this method to a phenotypic screening involving five drugs. The morphological attributes of three subcellular structures were summarized to consist a “fingerprint” describing the drug effect. The proposed method can provide comprehensive and detailed clues for drug evaluation， enriching the content of phenotypic screening.

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Distinct MLT asymmetry of auroral kilometric radiation observed by the FAST satellite

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Earth and Planetary Physics 2025年第1期9卷 188-193页

作者： JiaWen Tang Sai Zhang FuLiang Xiao HongMing Yang Si Liu YiHua He Chang Yang YuYue Jin ZhouKun deng ping Li Archie James Johnston School of Physics and Electronic Sciences Changsha University of Science and TechnologyChangsha 410114China Hunan Province Higher Education Key Laboratory of Modeling and Monitoring on the Near-Earth Electromagnetic Environments Changsha University of Science and TechnologyChangsha 410114China School of Electrical and Information Engineering Changsha University of Science and TechnologyChangsha 410114China

Auroral Kilometric Radiation (AKR) is a common radio emission,which can contribute to the magnetosphere-ionosphereatmosphere co u *** emissions have been observed in all magnetic planet magnetospheres of the solar *** this study,using observations from the FAST satellite from 30 August 1996 to 9 September 2001,the distribution of AKR in altitude=500-4500 km and invariant latitude (|ILAT|)=60°-80°has been analyzed.63045 AKR samples have been identified with~48%(52%) samples on the dayside (nightside).Of considerable interest,there is a distinct MLT asymmetry with the high occurrence rate in MLT=05-08 and 18-22(02-05 and 12-17) in the northern (southern) *** distinct MLT asymmetry is associated with the direction of Bxof the interplaneta ry magnetic *** addition,the occurrence rate on the nightside clearly increases as the AE^(*) index *** study further enriches the information and understanding of AKR in the magnetosphere as well as other similar radio emissions.

关键词： asymmetry auroral kilometric radiation interplanetary magnetic field