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Modulation of expression of P16 and Her2 in rat breast tissues of mammary hyperplasia model by external use of Rupifang Extract

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Journal of Traditional Chinese Medicine 2012年第4期32卷 651-656页

作者： Guijuan Zhang dehui Li Hui Guo Rui Liao Bizhu Tan Suyi Zhang Yubin Liu Min Ma Xiaoting Zeng Zhuolong peng Si Huang Yinghui Zheng Yi Ma The School Outpatient Department the First Affiliated Hospital of Jinan University Department of Traditional Chinese Medicine Medical College of Jinan University College of Life Science and Technology Jinan University

tract in external use on expression of proto-onco- genes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model. To explore the mechanisms of Rupifang Extract in external use for preventing and treating mammary hyperplasia. METHODS Thirty virginal female Wistar rats were randomized into 5 groups, 6 in each, A： blank con- trol group; B： model group; C： the low dose group of Rupifang; D： the middle dose group of Rupifang; and E： The high dose group of Rupifang. The mam- mary hyperplasia rat models were produced by in- jecting estradiol benzoate and progesterone and ir- ritating by tail nipping. Drug intervention was also launched during the model formation. After 30 days, the expression of her2 and p16 in breast tis- sues of rats in each group were detected by the SP immunohistochemical method. RESULTS： Compared with Blank control group, the expression of her2 in breast tissues in Model group was higher, and the expression of p16 was lower （P〈O.05 or P〈O.01）. After intervention with Rupi- fang Extract, compared with Model group, the ex- pression of her2 in breast tissues in Rupifang groups was lower, and the expression of p16 higher （P〈O.05 or P〈O.01）. CONCLUSION： The mechanisms of Rupifang Ex- tract in external application for preventing and treating mammary hyperplasia may be reducing the expression of proto-oncogenes her2 and in- creasing the expression of tumor suppression genes p16.

关键词： Fibrocystic Breast Disease Receptor,Epidermal Growth Factor Rupifang extract

A fluorescent film sensor for high-performance detection of Listeria monocytogenes via vapor sampling

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聚集体(英文) 2023年第1期4卷 165-172页

作者： Rongrong Huang Meiqi Li dehui Lin Yangtao Shao Congdi Shang Qianqian Liu Guijun Liu Nan Li Rong Miao Haonan peng Yanli Tang Yu Fang Key Laboratory of Applied Surface and Colloid Chemistry(Ministry of Education) School of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi’anChina Shaanxi Engineering Laboratory for Food Green Processing and Safety Control College of Food Engineering and Nutritional ScienceShaanxi Normal UniversityXi’anChina Department of Food Safety College of Food Science and EngineeringNorthwest A&F UniversityShaanxiChina

Foodborne Listeria monocytogenes poses serious threats to public *** and sensitive detection of the pathogen at the point of contamination is thus crucial to halt the spread of bacteria-related ***,we report for the first time a fluorescent film sensor to detect the biomarker of ***,*** sensor demonstrated unprecedented sensing performance for the analyte with a detection limit lower than 0.05 mg/m^(3),response time less than 1 s,full reversibility,and excellent *** study showed that the sensor can be used to monitor the growth of *** with much-improved *** superior performance of the sensor is ascribed to the specific binding,efficient charge transfer emission,and porous adlayer structure of the specially designed sensing fluorophore-based *** work paves the way to develop a portable detector to meet the needs for on-site and real-time detection of foodborne pathogens.

关键词： 3-hydroxy-2-butanone biomarkers fluorescent film sensors Listeria monocytogenes

Investigation of optimum discharge characteristics and chemical activity of AC driven air plasma jet and its anticancer effect

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Plasma Science and Technology 2021年第12期23卷 63-73页

作者： Sansan peng dehui XU Miao QI Rong LIU Xinying ZHANG Huaiyan ZHANG Bolun PANG Jin ZHANG Hao ZHANG Zhijie LIU Dingxin LIU State Key Laboratory of Electrical Insulation and Power Equipment Centre for Plasma BiomedicineXi'an Jiaotong UniversityXi'an 710049People's Republic of China The School of Life Science and Technology Xi'an Jiaotong UniversityXi'an 710049People's Republic of China

In this study,we investigated the effects of the quartz tube diameter,air flow rate,and applied voltage on the characteristics of an air plasma jet to obtain the optimized discharge *** physicochemical properties and concentration of reactive oxygen and nitrogen species(RONS)in plasma-activated medium(PAM)were characterized to explore their chemical ***,we investigated the inactivation effect of air plasma jet on tumour cells and their corresponding inactivation *** results show that the tube diameter plays an important role in sustaining the voltage of the air plasma jet,and the gas flow rate affects the jet length and discharge ***,the air plasma jet discharge displays two modes,namely,ozone and nitrogen oxide modes at high and low gas flow rates,*** the voltage increases the concentration of reactive species and the length of *** evaluating the viability of A549 cells under different parameters,the optimal treatment conditions were determined to be a quartz tube diameter of 4 mm,gas flow rate of 0.5 SLM,and voltage of 18 k ***,an air plasma jet under the optimized conditions effectively enhanced the chemical activity in PAM and produced more aqueous *** air plasma jet induced significant cytotoxicity in A549 cancer cells after plasma treatment.H_(2)O_(2) and NO_(2) are regarded as key factors in promoting cell *** present study demonstrates the potential use of tumour cell therapy by atmospheric air PAM,which aids a better understanding of plasma liquid chemistry.

关键词： cold atmospheric plasma discharge characteristics reactive species plasma-activated medium anticancer effect

不同药剂与施药时期对辣椒炭疽病防治效果的影响

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贵州农业科学 2024年第5期52卷 53-60页

作者：张爱民梁传静牟玉梅邢丹王永平周鹏涂德辉叶勇殷勇贵州省辣椒研究所贵州贵阳550006

【目的】探明贵州辣椒炭疽病的适宜防治药剂和防治时期,为辣椒炭疽病化学防控提供依据。【方法】选取已登记的15种辣椒炭疽病防治药剂单剂(25%咪鲜胺EC、80%波尔多液WP、50%肟菌酯WG、22.7%二氰蒽醌SC、70%代森锰锌WP、40%百菌清SC、30... 详细信息

【目的】探明贵州辣椒炭疽病的适宜防治药剂和防治时期,为辣椒炭疽病化学防控提供依据。【方法】选取已登记的15种辣椒炭疽病防治药剂单剂(25%咪鲜胺EC、80%波尔多液WP、50%肟菌酯WG、22.7%二氰蒽醌SC、70%代森锰锌WP、40%百菌清SC、30%琥胶肥酸铜WP、80%克菌丹WG、22.5%啶氧菌酯SC、85%三氯异氰尿酸SPX、40%苯醚甲环唑SC、500 g/L氟啶胺SC、50%咪鲜胺锰盐WP、250 g/L嘧菌酯SC和25%吡唑醚菌酯SC)和1个混剂(40%苯甲•吡唑酯SC)为药剂处理,选取贵州辣椒炭疽病的3个主要致病菌菌株(*** GL 17-25、*** GL 17-42和*** GL 17-147)为防治对象,采用菌丝生长抑制法和离体果实接种法对药剂进行初筛,通过田间试验进一步验证适宜的药剂和防治时期。【结果】25%咪鲜胺EC、50%咪鲜胺锰盐WP、70%代森锰锌WP对3种辣椒炭疽病菌的菌丝生长抑制率均为100.00%;离体果实接种试验表明,除70%代森锰锌WP外,各药剂的平均预防效果均高于治疗效果,25%咪鲜胺EC对3个菌株的预防和治疗效果最好,平均值分别为72.48%和60.01%,其次为25%吡唑醚菌酯SC(43.53%和30.36%)。相比发病中期用药,发病初期用药的病情指数低、防效高,25%吡唑醚菌酯SC和40%苯甲•吡唑酯SC的防效显著(P<0.05)高于25%咪鲜胺EC,前两者发病初期用药的防效分别为86.99%和88.59%,发病中期用药防效分别为50.87%和45.48%。【结论】25%吡唑醚菌酯SC和40%苯甲•吡唑酯SC能有效防治辣椒炭疽病,防治时期以发病初期为佳。

关键词：辣椒炭疽病药剂防治时期菌丝生长抑制率防效贵州

The Role of Ras-GTPase Activating Protein SH3Domain Binding Protein in Chilli Veinal Mottle Virus Infected Tobacco

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The Role of Ras-GTPase Activating Protein SH3Domain Binding ...

中国植物病理学会2023年学术年会

作者： peng Qiding CHENG Yongchao YUAN Bowen YANG Yiting XI dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life Sciences Sichuan University

Endogenous messenger RNAs( mRNAs) are exposed to a complex RNA regulatory network, which is a dynamic regulation in plant cells to maintain a delicate balance between active and inactive mRNAs in the development and fluctuating ***-binding proteins(RBPs) are the major regulators in the RNA regulatory network that control RNA processing in the nucleus and regulate gene expression in the cytoplasm at the post-transcriptional ***-GTPase activating protein SH3 domain binding protein( G3 BP) belongs to a family of RBPs and plays role in viral immunity in ***, the role of G3 BP in response to plant virus infection is largely ***, we showed that the expression of NtG3 BP-L could be first up-regulated and then down-regulated in chilli veinal mottle virus( ChiVMV)-infected Nicotiana tabacum *** overexpression of NbG3 BP-L( homologue of NtG3 BP-L) in Nicotiana benthamiana reduced the accumulation of *** result of yeast two-hybrid assay showed that NtG3 BP-L could specifically interact with6 K2 among the 11 proteins encoded by ***, the interaction between NtG3 BP-L and ChiVMV K2 was further confirmed by bimolecular fluorescence complementation(BiFC) and Co-Immunoprecipitation(Co-IP) *** assay showed that NtG3 BP-L and ChiVMV-6 K2 co-localized in cytoplasm and didn’t fuse at the *** localization also showed that more obvious fusion zone of NtG3 BP-L and ChiVMV-6 K2 appeared in cytoplasm under ChiVMV *** addition,*** plants with transgenic overexpression of NtG3 BP-L conferred resistance to ChiVMV infection,whereas the knockout of NtG3 BP-L in *** promoted ChiVMV ***, our results indicate that NtG3 BP-L plays a positive role in tobacco response to ChiVMV infection by interacting with ChiVMV-6 K2 and thereby affecting the infection of ChiVMV, which provide new insights into understanding the antiviral strategies of plants.

关键词：

The miR172/TOE3 Module Mediates the Susceptibility of Tobacco to Virus Infection by Regulating the Expression of PR1

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The miR172/TOE3 Module Mediates the Susceptibility of Tobacc...

中国植物病理学会2023年学术年会

作者： JIAO Bolei LIU Sucen peng Qiding ZHOU Jingya YUAN Bowen XI dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life Sciences Sichuan University

Transcription factors of the APETALA2( AP2) subfamily have been reported to be involved in plant growth, development and abiotic ***, little is known about the function of AP2 in plants response to virus *** this study, TARGET OF EAT3 of Nicotiana tabacum( NtTOE3), one of the clades of AP2 subfamily, was identified as a virus response gene in *** demonstrated that NtTOE3 played a positive role in attenuating virus infection in tobacco by NtTOE3 transgenic lines, including overexpression, RNA interference and knockout mutants of ***, we found that overexpression of NtTOE3 increased the expression of genes related to salicylic acid pathway in transgenic tobacco ***, we verified that NtTOE3 could activate the expression of NtPRl by binding to the promoter of *** of AP2 subfamily are targeted by miR172, so we speculated that miR172 might act as an upstream regulatory factor of *** expected, the result of qRT-PCR analysis showed that the expression of miR172 was correlated with changes of the expression of NtTOE3 in *** plants infected by tobacco mosaic virus( TMV).Importantly,we demonstrated that miR172 was a negative regulator of viral resistance in tobacco.5’ RACE assay was then employed to confirm that NtTOE3 was a target of *** evidence indicated that the miR172/TOE3 module enhanced susceptibility of tobacco to TMV infection by regulating the expression of *** conclusion, our findings reveal a role of miR172/TOE3 module in the regulation of plant immune system and elucidate a novel antiviral molecular mechanism.

关键词：

Effects of cucumber mosaic virus infection on physiological characteristic and gene expression of Panax notoginseng

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Effects of cucumber mosaic virus infection on physiological ...

中国植物病理学会2021年学术年会

作者： ZHENG Tianrui peng Qiding HUANG Wanying ZHOU Shuhao DONG Jiahong XI dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource Yunnan University of Chinese Medicine

Panax notoginseng（Burk.） ***,belonging to the Panax genus under the Araliaceae family,is one of the most highly valued medicinal plants in the *** saponins,the main medicinal active ingredients in *** are synthesized by secondary metabolic pathways and regulated by various *** yield and quality of *** are easily affected by various environmental factors,but the mechanism of the regulation is largely *** the expansion of *** artificial planting area,the occurrence of virus disease in *** is becoming *** this study,the effects of cucumber mosaic virus（CMV） on the growth and saponin synthesis of *** plants were *** results showed that the infection of CMV on *** plants resulted in vein yellow *** chlorophyll content and photosynthesis capacity of virus infected *** plants decreased significantly,whereas a large amount of reactive oxygen species accumulated and the activities of several antioxidant enzymes ***,cell damage and oxidative damage was more serious in virus infected *** *** RNA-Seq and transcriptomic analyses showed that many genes associated with triterpenoid saponin synthesis and disease resistance were up-regulated in *** plants under CMV *** together,our results indicated that virus infection could not only affect the growth of *** plants and induce the expression of defense related genes,but also promote the expression of genes related to the secondary metabolism of *** to improve the disease resistance of *** plants and make use of the up-regulation effect of terpenoid synthesis induced by virus infection is worthy of further study.

关键词： CMV Panax notoginseng Triterpene saponis

Identification of actinidia chlorotic ringspot-associated virus encoded P4 and P5 as RNA silencing suppressors

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Identification of actinidia chlorotic ringspot-associated vi...

中国植物病理学会2021年学术年会

作者： XU Qianyi peng Qiding HAN Hongyan WANG Yunru LI Xiangyangpeng XI dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University

Actinidia chlorotic ringspot-associated virus（AcCRaV,genus Emanavirus;family Fimoviridae） is a newly identified negative-stranded RNA virus infecting *** AcCRaV was first reported in China,it has showed a trend of rapid spread and become the main virus infecting kiwifruit in recent *** has a multipartite negative-stranded RNA genome with a single ORF encoded by each genomic *** this study,the functions of P4 and P5 encoded by RNA4 and RNA5 of AcCRaV were *** results of subcellular localization showed that P4 was located in the nucleus and cell membrane,whereas P5 was located only in the nucleus,indicating that P4 and P5 may play different roles in the process of AcCRaV infecting kiwifruit ***,putative proteins encoded by AcCRaV genomic RNA4 and RNA45 were screened for potential RNA silencing suppression activity by using a green fluorescent protein-based reporter agroinfiltration *** results showed that not only P5 had a local RNA silencing suppressor function,but P4,which was predicted as a movement protein,also had a local RNA silencing suppressor function,the results also showed that both P4 and P5 had activities of systemic RNA silencing *** investigation on the possible silencing suppressor active regions in P5 displayed that four partial segments of P5 had RNA silencing suppressor activity similar to that of the full length of ***,pathogenicity test showed that with the help of P4 or P5,potato virus X was more infectious,indicating that both P4 and P5 could enhance the pathogenicity of heterologous *** results provided new date of VSRs from negative-stranded RNA virus,and displayed that multiple VSRs may be essential for protecting AcCRaV from antiviral silencing in the perennial tree host,and may be related to the rapid epidemic of this virus.

关键词： AcCRaV Actinidia RNA silencing

A chain-type diamine strategy towards strongly anisotropic triiodide of DMEDA·I6

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Science China Materials 2020年第4期63卷 566-574页

作者： Li Yao peng Xu Wanru Gao Junze Li Liang Gao Guangda Niu dehui Li Shiyou Chen Jiang Tang Wuhan National Laboratory for Optoelectronics and School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China Research Institute for Magnetoelectronics&Weak Magnetic-field Detection College of ScienceChina Three Gorges UniversityYichang 443002China School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Precision Spectroscopy Key Laboratory of Polar Materials and Devices(MOE)and Department of OptoelectronicsEast China Normal UniversityShanghai 200241China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Linearly bonded triiodide chains with fairly small distance between the adjacent iodine ions feature a facile electron transfer and highly anisotropic ***,we demonstrate a novel strategy towards a new one-dimensional linear triiodide DMEDA·I6,using chain-type N,N'-dimethylethanediamine(DMEDA)cation to coordinate triiodine *** triiodide has the shortest distance between adjacent I3^- and good *** estimated electronic band gap of1.36 e V indicates its semiconducting properties.100 fold differences both in polarization-sensitive absorption and effective mass were achieved by simulation,with directions parallel and perpendicular to the a-axis of DMEDA·*** DMEDA·I6 single crystal-based photodetectors show a good switching characteristic and a distinct polarization-sensitive photoresponse with linear dichroic photodetection ratio of about *** anisotropic features and semiconducting properties of DMEDA·I6 make this triiodide system an interesting candidate for polarization related applications.

关键词： triiodide semiconductor polarization-sensitive detection linear dichroism