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Eosinophil MBP Extract Modulates Oncogene Expression in Prostate Tumor Cells: A Preliminary Study with Monolayer Cultures

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Journal of Cancer Therapy 2015年第6期6卷 482-492页

作者： Christine A. Clarke Michael A. Smith Ibrahim Laniyan Theresa R. Vaughn debra parish-gause William Green Paulette M. Furbert-Harris Department of Microbiology Howard University College of Medicine Washington DC USA Howard University Cancer Center Howard University College of Medicine Washington DC USA Carolina’s Hospital System Marion Mullins SC USA Department of Pathology Howard University College of Medicine Washington DC USA National Human Genome Center at Howard University Howard University College of Medicine Washington DC USA

Prostate cancer is the second leading cause of cancer deaths in the United States and remains a significant health concern for men throughout the world. Despite the discovery of promising immunotherapeutic strategies, curative outcomes remain elusive. We have investigated eosinophils as potential anti-cancer effector cells, and have reported the ability of their toxic granular proteins (MBP, EPO, ECP, EDN) to inhibit prostate tumor cell growth?in vitro. This study investigates the effect of eosinophil MBP extract on the expression of oncogenes p53, bcl-xl, bax, and c-myc, which modulate tumor growth, proliferation, and apoptosis. Briefly, granular proteins were differentially extracted from GRC.014.22 and GRC.014.24, eosinophilic cell lines established in our laboratory from a patient with moderate asthma. Protein extracts were fractionated on Sephadex G-50 columns, and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an African American patient) were treated with MBP extracts from the pooled third peaks. Colony formation and monolayer cell growth inhibition assays were used to evaluate the protein’s growth inhibitory activity against prostate tumor cells;and gene expression analyses, to determine p53, bcl-xl, bax, and c-myc oncogene expression. We show that the granular proteins were potent in their action on HPC8L, inhibiting colony formation in a dose-dependent manner. Treated prostate tumor cell lines trended toward apoptosis-induction, as evident in bcl-xl/bax ratios < 1, increased p53 expression, and up or downregulation of c-myc. These preliminary results demonstrate the growth inhibitory potential of eosinophil granular proteins and strongly support the hypothesis that eosinophils modulate the expression of oncogenes associated with prostate tumor proliferation and apoptosis. More importantly, this study offers insights into possible applications of eosinophilic mediators in oncogenic-targeted

关键词： Eosinophils Major Basic Protein (MBP) Prostate Cancer HPC8L Oncogenes

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Microglial dysfunction and genetic risk for neurodegenerative disease

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Neural Regeneration Research 2025年第5期20卷 1401-1402页

作者： debra S.MacDonald Jay Penney Department of Biomedical Sciences AVCUniversity of Prince Edward IslandCharlottetownPECanada

Neurodegenerative disorders such as Alzheimer's and Parkinson's diseases a re increasing in prevalence as world populations *** tremendous progress has been made,our understanding of the mechanisms that underlie the development of these diseases remains far from com *** troubling,despite the growing emotional and financial toll being to ken by neurodegenerative disorders,existing treatment options are limited almost exclusively to those that help manage symptoms but that lack the ability to alter the progression of the disease(Liu et al.,2022).

关键词： degenerative diseases

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Gene bank scheduling of seed regeneration: Interim report on a long term storage study

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Journal of Integrative Agriculture 2019年第7期18卷 1529-1540页

作者： Robert Redden debra Partington Australian Temperate Field Crops Collection Private Bag 260Agriculture Victoria

A major challenge for the management of gene banks is the maintenance of good seed health in the *** germplasm collections >10 000 accessions often have been acquired from different sources over a range of dates,may differ in germination at time of deposition in the gene bank,and may have genetic differences in seed *** major storage variables affecting seed longevity are temperature and seed moisture *** varieties of each pea(Pisum sativus L.),lentil(Lens culinaris Medikus ***),and chickpea(Cicer arietinum L.),were stored at three temperatures;40,20,and 2°C,each with three seed moisture levels of 10.9–13.8%(high),7.9–10.3%(medium),and 7–7.8%(low),in the Australian Temperate Field Crops Collection gene *** longevity at a given storage period was estimated by the corresponding germination percentage for each *** paper is an interim report on seed viability decline in the first seven years of this seed longevity study,in which viability decline towards zero was almost completed in the three seed moisture treatments at 40°C and the 20°C high seed moisture treatment,but had not declined in the other *** longevity positively responded to a reduction in temperature and then to a reduction in seed *** number of days in storage for seed germination decline to 85%(p85),and to 50%(p50) for mean seed viability,are reported by storage/varietal *** p85 and p50 showed significant inverse linear responses with seed moisture at 40°C for pea and lentil varieties,with intra-specific variation for *** long term trial aims to provide informed timing of seed regeneration for accessions in a gene bank.

关键词： gene bank storage moisture level temperature regeneration pea lentil chickpea

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Modulating Wnt signaling to improve cell replacement therapy for Parkinson＇s disease

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Journal of Molecular Cell Biology 2014年第1期8卷 54-63页

作者： Clare L. parish Lachlan H. Thompson Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville VIC 3010 Australia

Clinical trials have demonstrated the capacity for dopamine neurons, transplanted ectopicaUy into the striatum, to structurally inte- grate, restore dopamine transmission, and induce long-term functional benefits for Parkinson＇s disease （PD） patients. Despite this proof of principle, a number of limitations have hindered the development of cell replacement therapy over the past 20 years, particu- larly tissue availability, graft survival, and adequate reinnervation of the host brain. With a greater understanding of failure in prior clinical trials, increased knowledge of midbrain dopamine development （now including Wnts）, and the development of pluripotent stem cell technologies, we are better equipped than ever to re-address a number of these challenges. This review summarizes the trials, tribulations, and progress in cell replacement therapy for PD. We discuss the prospects of modulating canonical and non-canon- ical Wnt signalingto improve cell therapy based upon their roles in dopamine neural development and the adult brain. This will include the potential of Wnts to （i） expand fetaUy derived tissue in vitro and foUowing transplantation, （ii） promote the differentiation of pluripotent stem cells, （iii） increase graft integration and restoration of neural circuitry, and finally （iv） enhance graft survival.

关键词： Wnt dopamine Parkinson's disease cell transplantation neurogenesis axon growth motor function

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Tau's function and dysfunction in the brain:when small changes have big consequences

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Neural Regeneration Research 2024年第1期19卷 152-153页

作者： Miguel Portillo debra Toiber Department of Life Sciences Ben-Gurion University of the NegevBeer ShevaIsraelThe Zlotowski Center for NeuroscienceBen-Gurion University of the NegevBeer ShevaIsrael

With the increase of life expectancy and population growth,neurodegenerative diseases have risen too and are projected to be a major health public concern by *** diseases are characterized by the progressive decline of cognitive function leading to the subsequent loss of *** the underlying causes of neurodegeneration are not well understood,aging is the main risk factor.

关键词： diseases consequences function

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Control of hydrogen sulfide emissions using autotrophic denitrification landfill biocovers:engineering applications

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Frontiers of Environmental Science & Engineering 2011年第2期5卷 149-158页

作者： Daoroong SUNGTHONG debra R.REINHART Department of Environmental Science Faculty of ScienceSilpakorn UniversityNakhon Pathom73000Thailand Civil Environmental and Construction Engineering DepartmentUniversity of Central FloridaOrlandoFL 32826USA

Hydrogen sulfide(H_(2)S)emitted from construction and demolition waste landfills has received increasing *** its unpleasant odor,longterm exposure to a very low concentration of H_(2)S can cause a public health *** the case of construction and demolition(C&D)waste landfills,where gas collection systems are not normally required,the generated H_(2)S is typically not controlled and the number of treatment processes to control H_(2)S emissions in situ is *** attractive alternative may be to use chemically or biologically active landfill covers.A few studies using various types of cover materials to attenuate H_(2)S emissions demonstrated that H_(2)S emissions can be effectively *** this study,therefore,the costs and benefits of H_(2)S-control cover systems including compost,soil amended with lime,fine concrete,and autotrophic denitrification were *** on a case-study landfill area of 0.04 km^(2),the estimated H_(2)S emissions of 80900 kg over the 15-year period and costs of active cover system components(ammonium nitrate fertilizer for autotrophic denitrification cover,lime,fine concrete,and compost),ammonium nitrate fertilizer is the most cost effective,followed by hydrated lime,fine concrete,and yard waste *** concrete and yard waste compost covers are expensive measures to control H_(2)S emissions because of the large amount of materials needed to create a *** H_(2)S emissions using fine concrete and compost is less expensive at landfills that provide on-site concrete recovery and composting facilities;however,ammonium nitrate fertilizer or hydrated lime would still be more cost effective applications.

关键词： hydrogen sulfide emissions construction and demolition waste autotrophic denitrification landfill biocovers

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DNA damage and neurodegeneration:the unusual suspect

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Neural Regeneration Research 2017年第9期12卷 1441-1442页

作者： Daniel Stein debra Toiber Department of Life Sciences Ben-Gurion University of the Negev The Zlotowski Center for Neuroscience Ben-Gurion University of the Negev

Neurodegenerative diseases are caused by the loss of neuro- nal cells. As neurons deteriorate, a patient may experience relatively mild symptoms such as failing to name objects and people. However, with the progression of the disease, symp- toms worsen resulting in loss of independence and a sense of＂loss of oneself＂. Finally, the patient dies from a variety of complications.

关键词： DNA damage and neurodegeneration:the unusual suspect

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Pathologic pancreatic endocrine cell hyperplasia

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World Journal of Gastroenterology 2011年第2期17卷 137-143页

作者： debra Ouyang Deepti Dhall Run Yu Department of Medicine Cedars-Sinai Medi-cal Center Los Angeles CA 90048 United States Department of Pathology Cedars-Sinai MedicalCenter Los Angeles CA 90048 United States Division of Endocrinology and Carcinoid and Neu-roendocrine Tumor Center Cedars-Sinai Medical Center LosAngeles CA 90048 United States

Pathologic hyperplasia of various pancreatic endocrine cells is rare but has been long known.β cell hyperplasia contributes to persistent hyperinsulinemic hypoglycemia of infancy,which is commonly caused by mutations in the islet ATP-sensitive potassium channel,and to noninsulinoma pancreatogenous hypoglycemia in adults,which may or may not be associated with bariatric surgery.α cell hyperplasia may cause glucagonoma syndrome or induce pancreatic neuroendocrine *** inactivating mutation of the glucagon receptor causes α cell hyperplasia and asymptomatic *** polypeptide cell hyperplasia has been described without a clearly-characterized clinical syndrome and hyperplasia of other endocrine cells inside the pancreas has not been reported to our knowledge. Based on morphological evidence,the main pathogenetic mechanism for pancreatic endocrine cell hyperplasia is increased endocrine cell neogenesis from exocrine ductal *** endocrine cell hyperplasia should be considered in the diagnosis and management of hypoglycemia,elevated islet hormone levels,and pancreatic neuroendocrine *** studies of pathologic pancreatic endocrine cell hyperplasia will likely yield insights into the pathogenesis and treatment of diabetes and pancreatic neuroendocrine tumors.

关键词： Glucagon receptor Hyperplasia Nesidioblastosis Islet Pancreatic endocrine cell Neuroendocrine tumor

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Safe acupuncture and dry needling during pregnancy:New Zealand physiotherapists' opinion and practice

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Journal of Integrative Medicine 2019年第1期17卷 30-37页

作者： Jillian Marie McDowell Susan Heather Kohut debra Betts Prohealth Physiotherapy School of Clinical Sciences Faculty of Health and Environmental Sciences Auckland University of Technology New Zealand School of Acupuncture and Traditional Chinese Medicine

Objective: Acupuncture guidelines have advised caution when treating women during pregnancy,because historical ‘‘forbidden" acupuncture points are believed to stimulate miscarriage or early *** recent research demonstrating that acupuncture is a useful and safe treatment tool for pregnancy-related low-back pain(LBP) and pelvic girdle pain(PGP), it is postulated that fear of miscarriage and subsequent blame by association, restricts its provision. More recently, an increase in dry needling(DN) courses for physiotherapists has potentiated the rapid growth in DN practice in New Zealand(NZ). Many dry needlers do not consider DN to be a form of acupuncture; it is unknown if they have similar safety ***: NZ registered physiotherapists practicing acupuncture and/or DN were invited to participate in an electronic survey to examine their practice and level of understanding in regard to safe needling during ***: Of 124 respondents, only 60(48%) would needle pregnant women, with a further 66% of those still expressing safety concerns. NZ physiotherapists practicing DN only, were more likely to needle areas related to ‘‘forbidden" points in all trimesters. However, overall, NZ physiotherapists were less likely to needle ‘‘forbidden" points than their UK ***: Conflicting literature and a ‘‘fear of blame" influences NZ physiotherapists' decisions to offer needling(both acupuncture and DN) during pregnancy. Further training in this field is recommended to ensure safe practice and adequate provision of acupuncture treatment options for pregnant women suffering musculoskeletal pain, such as LBP and PGP. Further research, particularly into DN, for women during pregnancy, is warranted.

关键词： Physiotherapy Acupuncture Dry needling Safety Pregnancy Trigger point therapy

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Drug development guidelines for Hepatitis C virus——From a Clinical Perspective

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Journal of Chinese Pharmaceutical Sciences 2014年第5期23卷 340-342页

作者： debra Birnkrant Division of Antiviral Products in the Office of Antimicrobial Products CDER’s Office of New Drugs

Chronic hepatitis C virus infection is a global public health *** is estimated that 170-200 million persons around the world are infected with the hepatitis C virus;it affects about 3 to 5 million people in the United *** people infected with the hepatitis C virus(HCV)are not aware of their condition so they do not seek or receive *** 75 percent of those with HCV infection in the

关键词：《中国药学》期刊编辑工作发行工作

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