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Science Letters:Transient expression of chicken alpha interferon gene in lettuce

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第5期9卷 351-355页

作者： Li SONG de-gang zhao Yong-jun WU Yi LI Guizhou Key Laboratory of Ago-Bioengineering Guizhou University Guiyang 550025 China Ministry of Education Key Laboratory of Green Pesticide and Ago-Bioengineering Guizhou University Guiyang 550025 China College of Life Sciences Guizhou University Guiyang 550025 China department of Plant Science University of Connecticut Storrs CT 06269 USA

We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.

关键词： Chicken alpha interferon (ChlFN-α) Expression Transgenic lettuce Bioactivity

Glass-forming ability, microhardness, corrosion resistance, and dealloying treatment of ***40Ndx alloy ribbons

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International Journal of Minerals,Metallurgy and Materials 2017年第6期24卷 708-717页

作者： Hao-yi Chi Zhen-gui Yuan Yan Wang Min Zuo de-gang zhao Hao-ran Geng Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials School of Materials Science and Engineering University of Jinan Jinan 250022 China

The influence of Nd addition on the glass-forming ability (GFA), microhardness, and corrosion resistance of Mg60−xCu40Ndx(x = 5, 10, 15, 20, and 25, at%) alloys were investigated by differential scanning calorimetry, Vickers-type hardness tests, and electrochemical methods. The results suggest that the GFA and microhardness of the amorphous alloys increase until the Nd content reaches 20at%. The corrosion potential and corrosion current density obtained from the Tafel curves indicate that the Mg35Cu40Nd25ternary alloy exhibits the best corrosion resistance among the investigated alloys. Notably, nanoporous copper (NPC) was synthesized through a single-step dealloying of Mg60−xCu40Ndx(x = 5, 10, 15, 20, and 25) ternary alloys in 0.04 mol·L−1H2SO4solution under free corrosion conditions. The influence of dealloying process parameters, such as dealloying time and temperature, on the microstructure of the ribbons was also studied using the surface diffusivity theory. The formation mechanism of dealloyed samples with a multilayered structure was also discussed. © 2017, University of Science and Technology Beijing and Springer-Verlag Berlin Heidelberg.

关键词： Corrosion Corrosion resistance Differential scanning calorimetry Glass Microhardness Neodymium alloys Ternary alloys

Acoustic topological adiabatic passage via a level crossing

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Science China(Physics,Mechanics & Astronomy) 2021年第4期64卷 2-9页

作者： Ya-Xi Shen Long-Sheng Zeng Zhi-Guo Geng de-gang zhao Yu-Gui Peng Jie Zhu Xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and TechnologyWuhan 430074China department of Mechanical Engineering Hong Kong Polytechnic UniversityHong KongChina Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen 518057China

Stimulated adiabatic passage has been extensively studied to achieve robust and selective population transfer in quantum systems. Recently, the quantum-classic analogy has been rapidly developing and can be considered responsible for the implementation of the adiabatic transfer of sound energy in cavity chain systems. In this article, we investigate the adiabatic transfer of sound energy between two topological end states in the Su-Schrieffer-Heeger(SSH) cavity chain, which can be considered to be the acoustic analog of the quantum chirped-pulse excitation. The topological adiabatic passage in SSH cavity chain has two categories. When the single-cavity resonance frequencies on the sublattices A and B in the SSH cavity chain do not switch their spectrum positions, the topologically protected adiabatic evolution results in the returning passage of the sound excited in one end cavity. When a level crossing with single-cavity resonance frequencies on the sublattices A and B exhibits switch in the frequency spectrum, acoustic energy is observed to be topologically pumped between the two end cavities of the SSH chain.

关键词： acoustic adiabatic passage topological end state level crossing

Overview of Stachybotrys(Memnoniella)and current species status

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Fungal Diversity 2015年第2期71卷 17-83页

作者： Yong Wang Kevin D.Hyde Eric H.C.McKenzie Yu-Lan Jiang de-Wei Li de-gang zhao Guizhou Key Laboratory Agro-Bioengineering Guizhou UniversityGuiyangGuizhou 550025People’s Republic of China department of Plant Pathology College of AgricultureGuizhou UniversityGuiyangGuizhou 550025China Institute of Excellence in Fungal Research School of ScienceMae Fah Luang UniversityChiang RaiThailand Landcare Research Private Bag 92170AucklandNew Zealand Valley Laboratory The Connecticut Agricultural Experiment Station153 Cook Hill RoadWindsorCT 06095USA

Stachybotrys(asexual Hypocreales)has a worldwide *** genus inhabits substrates rich in cellulose and is closely related to *** of species has previously been based on morphology,with conidial characters being considered as *** study reevaluates Stachybotrys and Memnoniella,which is shown to include at least seven species-groups;while Memnoniella is a synonym of *** sexual genera Ornatispora and Melanopsamma are also synonyms of *** the exception of Stachybotrys subsimplex,species formed a wellsupported monophyletic group in LSU data analysis belonging to ***-four accepted Stachybotrys species are discussed,while eight species are considered to belong to other genera or are doubtful in this paper and a key to these species *** new combinations and 1 nomina nova is *** status of Stachybotrys species on health,as human or animal pathogens,in indoor environments,and use as biocontrol agents and compound discovery are also discussed.

关键词： Current species Nomen excludendum Phylogeny Synonyms Taxonomic history

A MOVPE method for improving InGaN growth quality by pre-introducing TMIn

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Chinese Physics B 2021年第1期30卷 551-554页

作者： Zi-Kun Cao de-gang zhao Jing Yang Jian-Jun Zhu Feng Liang Zong-Shun Liu State Key Laboratory of Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China College of Materials Science and Opto-Electronic Technology University of Chinese Academy of SciencesBeijing 100049China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

We propose a metal organic vapor phase epitaxy(MOVPE) method of pre-introducing TMIn during the growth of uGa N to improve the subsequent growth of In Ga N and discuss the impact of this method in detail. Monitoring the MOVPE by the interference curve generated by the laser incident on the film surface, we found that this method avoided the problem of the excessive In Ga N growth rate. Further x-ray diffraction(XRD), photoluminescence(PL), and atomic force microscope(AFM) tests showed that the quality of In Ga N is improved. It is inferred that by introducing TMIn in advance, the indium atom can replace the gallium atom in the reactor walls, delivery pipes, and other corners. Hence the auto-incorporation of gallium can be reduced when In Ga N is grown, so as to improve the material quality.

关键词： InGaN metal organic vapor phase epitaxy(MOVPE)

Diversity of non-Laboulbenialean fungi on millipedes

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Studies in Fungi 2017年第1期2卷 130-137页

作者： Enghoff H Reboleira ASPS de-gang zhao Natural History Museum of denmark University of CopenhagenUniversitetsparken 15DK-2100 Copenhagen OEDenmark 不详

New records are given for secondary capilliconidia of Basidiobolus from several species of millipedes(Diplopoda)belonging to three different *** anamorph‘Thaxteriola‟stage of Rhynchomyces is recorded(with doubt)from a Brazilian millipede belonging to the order *** enigmatic fungus showing characteristics of Coreomycetopsis,Hormiscioideus and Antennopsis is recorded from two species of Danish millipedes of the order *** structures,tentatively referred to fungi are recorded from several millipede orders where they occur between micro-scutes of the external cuticle.

关键词： Amphoromorpha Antennopsis Ascomycota Basidiobolus Coreomycetopsis Diplopoda Hormiscioideus Mucoromycotina Pyxidiophora Rhynchomyces Zygomycota

Evaluation of polarization field in InGaN/GaN multiple quantum well structures by using electroluminescence spectra shift

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Chinese Physics B 2020年第3期29卷 221-224页

作者： Ping Chen de-gang zhao de-Sheng Jiang Jing Yang Jian-Jun Zhu Zong-Shun Liu Wei Liu Feng Liang Shuang-Tao Liu Yao Xing Li-Qun Zhang State Key Laboratory of Integrated Optoelectronics Institute of SemiconductorsChinese Academy of Sciences(CAS)Beijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Nano-devices and Applications of CAS Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China

In order to investigate the inherent polarization intensity in InGaN/GaN multiple quantum well(MQW) structures,the electroluminescence(EL) spectra of three samples with different GaN barrier thicknesses of 21.3 nm, 11.4 nm, and 6.5 nm are experimentally studied. All of the EL spectra present a similar blue-shift under the low-level current injection,and then turns to a red-shift tendency when the current increases to a specific value, which is defined as the turning *** value of this turning point differs from one another for the three InGaN/GaN MQW samples. Sample A, which has the GaN barrier thickness of 21.3 nm, shows the highest current injection level at the turning point as well as the largest value of blue-shift. It indicates that sample A has the maximum intensity of the polarization field. The red-shift of the EL spectra results from the vertical electron leakage in InGaN/GaN MQWs and the corresponding self-heating effect under the high-level current injection. As a result, it is an effective approach to evaluate the polarization field in the InGaN/GaN MQW structures by using the injection current level at the turning point and the blue-shift of the EL spectra profiles.

关键词： InGaN/GaN multiple quantum well(MQW) polarization field electroluminescence spectra shift electron leakage current

Interference of Quinolinate Phosphoribosyltransferase Gene QPT Affects Agronomic Traits and Leaf Quality in Nicotiana tabacum L.

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Journal of Agricultural Science and Technology(B) 2020年第4期10卷 229-245页

作者： Chun Wei Zhi-Hui Du Li-Jun Qin de-gang zhao Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) Collaborative Innovation Center for Mountain Ecology&Agro-bioengineering(CICMEAB)College of Life Sciences/Institute of Agro-bioengineeringGuizhou UniversityGuiyang 550025Guizhou ProvinceChina Guizhou Academy of Agricultural Sciences Guiyang 550025Guizhou ProvinceChina

Quinolinate phosphoribosyltransferase(QPRTase),a key enzyme in ensuring nicotinic acid is available for the synthesis of defensive pyridine alkaloids in Nicotiana species,also plays an important role in nicotinamide adenine dinucleotide(NAD)*** this study,the morphological traits,the quality characteristics and photosynthetic parameters in QPT-overexpressing/interfering tobacco plants were investigated,*** showed that the interference of QPT gene not only reduced significantly the morphological traits including plant height,stem girth,leaf number and leaf length,*** 20 days after transplanting(DAT),but the flowering period was delayed 10-15 d in interfered tobaccos compared with the overexpressed,control and wild-type ***,at 40 DAT and 60 DAT,only three indexes(plant height,stem girth and leaf number)in QPT-interfering plants appeared significant difference in comparison with other three types of tobacco ***,the determination results from nicotine,sugar,K+and Cl-content showed the nicotine content in interfered plants was always significantly lower than that in overexpressed plants,control and the wild-type ones respectively whatever toppling or *** the same time,the toppling treatment also caused the increasement of K+content among the four different tobacco lines,but the maximum increase amplitude of K+content was found in QPT-overexpressing tobaccos while the minimum appeared in QPT-interfering ***,QPT-interference in transgenic tobaccos likewise affected the photosynthesis by reducing net photosynthetic rate(Pn),stomata conductance(Gs)and transpiration rate(Tr),while there was no significant difference between QPT-overexpressing plants and the controls and the wild-types.

关键词： Nicotiana tabacum QPT gene physiological characteristics tobacco quality

Comparative transcriptome and co-expression analysis reveal key genes involved in leaf margin serration in Perilla frutescens

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Chinese Herbal Medicines 2020年第3期12卷 265-272页

作者： Qi Shen Dong Zhang Tian-yuan Zhang Yang-yang Xu de-gang zhao Institute of Medical Plant Physiology and Ecology School of Pharmaceutical SciencesGuangzhou University of Chinese MedicineGuangzhou 510006China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China Guizhou Academy of Agricultural Sciences Guiyang 550008China China Center for Information Industry development Beijing 100036China

Objective:In this study,we aimed to identify the genes involved in leaf margin serration in Perilla ***(Family:Lamiaceae)is widely grown in Asian *** leaf is the medicinal part stipulated in the Chinese *** are mainly two types of perilla leaves:one with serrated leaf margin which is the phenotype described in the pharmacopoeia and the other with smooth leaf ***:Transcriptome sequencing,co-expression analysis,and qRT-PCR analysis of six perilla tissues sampled from two different phenotypes(serrated and smooth leaves)were ***:Forty-three differentially expressed genes(deGs),which may potentially regulate leaf shape,were identified through de novo transcriptome sequencing between the two *** involved in leaf shape regulation were ***,we validated five deGs by qRT-PCR,and the results were consistent with the transcriptome *** addition,1186 transcription factors(TFs)belonging to 45 TF families were ***,the co-expression network of deGs was ***:The study identified the key genes that control leaf shape by comparing the *** findings also provide basic data for further exploring ***,which can help study the mechanism of leaf shape development and molecular breeding.

关键词： leaf shape development Perilla frutescens(L.)Britt. transcriptome sequencingw rinkly leaves