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A high-throughput study on endothelial cell adhesion and growth mediated by adsorbed serum protein via signaling pathway PCR array

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Regenerative Biomaterials 2018年第1期5卷 25-34页

作者： Xiaoying Lü Yayun Qu Ying Hong Yan Huang Yiwen Zhang dayun Yang Fudan Zhang Tingfei Xi Deyuan Zhang State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096P.R.China Nanjing Drum Tower Hospital Nanjing 210008P.R.China SQ Medical Device Co. Ltd.Nanjing 210008P.R.China Shenzhen Research Institute Peking UniversityShenzhen 518055P.R.China R&D Center of Lifetech Scientific(Shenzhen)Co. LtdShenzhen 518057P.R.China

The purpose of this paper is to utilize the signaling pathway polymerase chain reaction(PCR)arrays to investigate the activation of two important biological signaling pathways in endothelial cell adhesion and growth mediated by adsorbed serum protein on the surface of bare and titanium nitride(TiN)-coated nickel titanium(NiTi)alloys.First,the endothelial cells were cultured on the bare and TiN-coated NiTi alloys and chitosan films as control for 4 h and 24 h,respectively.Then,the total RNA of the cells was collected and the PCR arrays were performed.After that,the differentially expressed genes in the transforming growth factor beta(TGF-b)signaling pathway and the regulation of actin cytoskeleton pathway were screened out;and the further bioinformatics analyses were performed.The results showed that both TGF-b signaling pathway and regulation of actin cytoskeleton pathway were activated in the cells after 4 h and 24 h culturing on the surface of bare and TiN-coated NiTi alloys compared to the chitosan group.The activated TGF-b signaling pathway promoted cell adhesion;the activated regulation of actin cytoskeleton pathway promoted cell adhesion,spreading,growth and motility.In addition,the activation of both pathways was much stronger in the cells cultured for 24 h versus 4 h,which indicated that cell adhesion and growth became more favorable with longer time on the surface of two NiTi alloy materials.

关键词： biomaterials protein adsorption and cell adhesion signaling pathway PCR array biological signaling pathway

A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies

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Plant Diversity 2020年第5期42卷 370-375页

作者： Mianmian Wang Jun yang Jinpeng Wan dayun Tao Jiawu Zhou Diqiu Yu Peng Xu Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunming650223YunnanChina University of Chinese Academy of Sciences Beijing100049China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMengla666303YunnanChina Yunnan Key Laboratory for Rice Genetic Improvement Food Crops Research InstituteYunnan Academy of Agricultural SciencesKunming650200YunnanChina

Interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving yields.However,interspecific hybridization is often hindered by hybrid sterility,linkage drag,and distorted segregation.To mine for favorable genes from Oryza glaberrima,we cultivated a series of BC4 introgression lines(ILs)of O.glaberrima in the japonica rice variety background(Dianjingyou 1)in which the IL-2769(BC4F10)showed longer sterile lemmas,wider grains and spreading panicles compared with its receptor parent,suggesting that linkage drag may have occurred.Based on the BC5F2 population,a hybrid sterility locus,S20,a long sterile lemma locus,G1-g,and a new grain width quantitative trait locus(QTL),qGW7,were mapped in the linkage region about 15 centimorgan(cM)from the end of the short arm of chromosome 7.The hybrid sterility locus S20 from O.glaberrima eliminated male gametes of Oryza sativa,and male gametes carrying the alleles of O.sativa in the heterozygotes were aborted completely.In addition,the homozygotes presented a genotype of O.glaberrima,and homozygous O.sativa were not produced.Surprisingly,the linked traits G1-g and qGW7 showed similar segregation distortion.These results indicate that S20 was responsible for the linkage drag.As a large number of detected hybrid sterility loci are widely distributed on rice chromosomes,we suggest that hybrid sterility loci are the critical factors for the linkage drag in interspecific and subspecific hybridization of rice.

关键词： Interspecific hybridization Linkage drag Hybrid sterility Segregation distortion

Natural alleles of a uridine 5'-diphospho-glucosyltransferase gene responsible for differential endosperm development between upland rice and paddy rice

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Journal of Integrative Plant Biology 2022年第1期64卷 135-148页

作者： Zihao Wu Xiao Zhang Guimei Chang Jun yang Jinpeng Wan Feijun Wang dayun Tao Jiawu Zhou Lianguang Shang Peng Xu Diqiu Yu School of Life Sciences University of Science and Technology of ChinaHefei 230027China CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical Gardenthe Chinese Academy of SciencesKunming 650223China Yunnan Key Laboratory for Rice Genetic Improvement Food Crops Research InstituteYunnan Academy of Agricultural SciencesKunming 650200China The Innovative Academy of Seed Design Chinese Academy of SciencesMengla 666303China Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agricultural and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMergla 666303China State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan UniversityKunming 650091China

Traditional upland rice generally exhibits insufficient grains resulting from abnormal endosperm development compared to paddy rice. However, the underlying molecular mechanism of this trait is poorly understood. Here,we cloned the uridine 5’-diphospho(UDP)-glucosyltransferase gene EDR1(Endosperm Development in Rice) responsible for differential endosperm development between upland rice and paddy rice by performing quantitative trait loci analysis and map-based cloning. EDR1 was highly expressed in developing seeds duringgrain filling. Natural variations in EDR1 significantly reduced the UDP-glucosyltransferase activity of EDR1 YZNcompared to EDR1 YD1,resulting in abnormal endosperm development in the near-isogenic line, accompanied by insufficient grains and changes in grain quality.By analyzing the distribution of the two alleles EDR1 YD1 and EDR1 YZNamong diverse paddy rice and upland rice varieties, we discovered that EDR1 was conserved in upland rice, but segregated in paddy rice. Further analyses of grain chalkiness in the alleles of EDR1 YD1 and EDR1 YZNvarieties indicated that rice varieties harboring EDR1 YZNand EDR1 YD1 preferentially showed high chalkiness, and low chalkiness,respectively. Taken together, these results suggest that the UDP-glucosyltransferase gene EDR1 is an important determinant controlling differential endosperm development between upland rice and paddy rice.

关键词： EDR1 endosperm development grain quality UDP-glucosyltransferase upland rice

Salidroside Protects Against 6-Hydroxydopamine-Induced Cytotoxicity by Attenuating ER Stress

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Neuroscience Bulletin 2016年第1期32卷 61-69页

作者： Kai Tao Bao Wang dayun Feng Wei Zhang Fangfang Lu Juan Lai Lu Huang Tiejian Nie Qian yang Department of Neurosurgery Tangdu Hospital The Fourth Military Medical University Research Center of Traditional Chinese Medicine Xijing Hospital The Fourth Military Medical University

Parkinson＇s disease （PD） is a neurodegenera- tive disease characterized by a persistent decline of dopaminergic （DA） neurons in the substantia nigra pars compacta. Despite its frequency, effective therapeutic strategies that halt the neurodegenerative processes are lacking, reinforcing the need to better understand the molecular drivers of this disease. Importantly, increasing evidence suggests that the endoplasmic reticulum （ER） stress-induced unfolded protein response is likely involved in DA neuronal death. Salidroside, a major compound isolated from Rhodiola rosea L., possesses potent anti- oxidative stress properties and protects against DA neu- ronal death. However, the underlying mechanisms are not well understood. In the present study, we demonstrate that salidroside prevents 6-hydroxydopamine （6-OHDA）- induced cytotoxicity by attenuating ER stress. Further- more, treatment of a DA neuronal cell line （SN4741） and primary cortical neurons with salidroside significantly reduced neurotoxin-induced increases in cytoplasmic reactive oxygen species and calcium, both of which cause ER stress, and cleaved caspase-12, which is responsible for ER stress-induced cell death. Together, these results sug- gest that salidroside protects SN4741 cells and primary cortical neurons from 6-OHDA-induced neurotoxicity by attenuating ER stress. This provides a rationale for the investigation of salidroside as a potential therapeutic agent in animal models of PD.

关键词： Parkinson＇s disease - ER stress Salidroside6-OHDA Neuroprotection

Genomic divergence in cotton germplasm related to maturity and heterosis

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Journal of Integrative Plant Biology 2019年第8期61卷 929-942页

作者： Shoupu He Gaofei Sun Longyu Huang Daigang yang Panhong Dai dayun Zhou Yuzhen Wu Xiongfeng Ma Xiongming Du Shoujun Wei Jun Peng Meng Kuang Research Base State Key Laboratory of Cotton BiologyAnyang Institute of TechnologyAnyang 455000China Institute of Cotton Research of the Chinese Academy of Agricultural Sciences Anyang 455000China Department of Computer Science and Information Engineering/Data Mining Institute Anyang Institute of TechnologyAnyang 455000China

Commercial varieties of upland cotton(Gossypium hirsutum)have undergone extensive breeding for agronomic traits,such as fiber quality,disease resistance,and yield.Cotton breeding programs have widely used Chinese upland cotton source germplasm(CUCSG)with excellent agronomic traits.A better understanding of the genetic diversity and genomic characteristics of these accessions could accelerate the identification of desirable alleles.Here,we analyzed 10,522 high-quality singlenucleotide polymorphisms(SNP)with the CottonSNP63 K microarray in 137 cotton accessions(including 12 hybrids of upland cotton).These data were used to investigate the genetic diversity,population structure,and genomic characteristics of each population and the contribution of these loci to heterosis.Three subgroups were identified,in agreement with their knownpedigrees,geographical distributions,and times since introduction.For each group,we identified lineagespecific genomic divergence regions,which potentially harbor key alleles that determine the characteristics of each group,such as early maturity-related loci.Investigation of the distribution of heterozygous loci,among 12 commercial cotton hybrids,revealed a potential role for these regions in heterosis.Our study provides insight into the population structure of upland cotton germplasm.Furthermore,the overlap between lineagespecific regions and heterozygous loci,in the high-yield hybrids,suggests a role for these regions in cotton heterosis.

关键词： upland cotton fiber quality genetic diversity

Raf激酶抑制蛋白在非小细胞肺癌中的表达及其临床意义

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中国肺癌杂志 2012年第10期15卷 597-601页

作者：杨大运齐战河北医科大学第四医院内科石家庄050011 河北医科大学第四医院胸外科石家庄050011

背景与目的 Raf激酶抑制蛋白(Rafkinase inhibitor protein,RKIP)属于磷脂酰乙醇胺结合蛋白家族的成员,RKIP参与ERK/MAPK、G蛋白偶联受体和NF-κΒ等信号传导过程,且RKIP的表达减弱或丢失与多种肿瘤的发生发展及侵润转移相关。本研究旨... 详细信息

背景与目的 Raf激酶抑制蛋白(Rafkinase inhibitor protein,RKIP)属于磷脂酰乙醇胺结合蛋白家族的成员,RKIP参与ERK/MAPK、G蛋白偶联受体和NF-κΒ等信号传导过程,且RKIP的表达减弱或丢失与多种肿瘤的发生发展及侵润转移相关。本研究旨在探讨RKIP在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其与NSCLC临床病理特征的相关性。方法应用RT-PCR、Western blot及免疫组化方法检测83例NSCLC及其癌旁组织标本中RKIP的表达,并结合临床病理学资料进行统计学分析,所有病例均经病理诊断确诊,均无其它部位原发肿瘤,术前无化疗、放疗和免疫治疗史。结果 NSCLC中RKIP mRNA及蛋白的表达明显低于癌旁组织,差异有统计学意义(P0.05)。结论 RKIP的低表达与NSCLC的发生及侵袭转移有关,可作为NSCLC预测及预后评估的指标。

关键词：肺肿瘤 Raf激酶抑制蛋白 Western blot RT-PCR 免疫组织化学

Influence of Co, W and Ti on the Stress-rupture Lives of a Single Crystal Nickel-base Superalloy

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Journal of Materials Science & Technology 2006年第2期22卷 169-172页

作者： dayun Yang Tao JIN Nairen ZHAO Zhihui WANG Xiaofeng SUN Hengrong GUAN Zhuangqi HU Department of Superalloy Institute of Metal Research Chinese Academy of Sciences Shenyang 110016 China

压力破裂上的公司， W 和 Ti 的影响 aNi-Cr-AI-Mo-Ta-Co-W-Ti 单身者水晶镍底生活超级合金用 L_9 (~) 被调查了由统计分析的直角的数组设计(OAD ) 。在一个选择作文范围， Ti 内容是到压力破裂生活的效果的最重要的因素然后由 Coconte... 详细信息

压力破裂上的公司， W 和 Ti 的影响 aNi-Cr-AI-Mo-Ta-Co-W-Ti 单身者水晶镍底生活超级合金用 L_9 (~) 被调查了由统计分析的直角的数组设计(OAD ) 。在一个选择作文范围， Ti 内容是到压力破裂生活的效果的最重要的因素然后由 Cocontent 列在后面。W 内容在压力破裂生命上有最小的效果。最佳的合金 contain10 wt 应该爱抚公司， 8 wt 宠物 W 和零 Ti。优化合金也为 500 h 在 870 deg C 在热暴露期间有好微结构稳定性。

关键词：单晶超耐热合金镍合金正交设计应力裂变钴钨

新型含铝奥氏体不锈钢在超临界水环境下的腐蚀行为

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核动力工程 2023年第5期44卷 244-250页

作者：孙达云高阳张乐福韩忠立郭相龙上海交通大学核科学与工程学院上海200240

为了丰富含铝奥氏体不锈钢(AFAs)在超临界水环境下的腐蚀行为研究数据,支持超临界水冷堆(SCWR)包壳材料评估工作,本研究对自主设计的AFAs进行600℃/25 MPa的超临界水腐蚀实验,结合微观分析表征手段分析其腐蚀行为。结果表明,本研究所用A... 详细信息

为了丰富含铝奥氏体不锈钢(AFAs)在超临界水环境下的腐蚀行为研究数据,支持超临界水冷堆(SCWR)包壳材料评估工作,本研究对自主设计的AFAs进行600℃/25 MPa的超临界水腐蚀实验,结合微观分析表征手段分析其腐蚀行为。结果表明,本研究所用AFAs在腐蚀1000 h后的增重达34 mg/dm^(2),约为相同条件下的310S钢的腐蚀增重的一半。AFAs表面形成了双层氧化物,外层主要为Fe_(2)O_(3)和富Ni尖晶石,内层主要为Cr_(2)O_(3)。氧化铝以离散的颗粒形式存在于内层,阻碍扩散过程的同时可以作为Cr_(2)O_(3)的形核位点,促进Cr_(2)O_(3)的形成。因此,本研究所用AFAs在该实验条件下表现出优异的耐腐蚀性能。

关键词：含铝奥氏体不锈钢超临界水腐蚀 310S钢

植被恢复模式对土壤团聚体和有机碳储量的影响

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森林与环境学报 2022年第6期42卷 631-639页

作者：杨倩朱大运陈静陈海贵州师范大学喀斯特研究院贵州贵阳550001 国家喀斯特石漠化防治工程技术研究中心贵州贵阳550001

为探究喀斯特石漠化区不同的植被恢复模式对土壤团聚体稳定性和土壤有机碳储量产生的影响,以高原山地喀斯特石漠化区3种典型植被恢复模式(草地、林草地和林灌草地)下的土壤为研究对象,分别用干筛法和湿筛法研究不同年份、不同处理方法... 详细信息

为探究喀斯特石漠化区不同的植被恢复模式对土壤团聚体稳定性和土壤有机碳储量产生的影响,以高原山地喀斯特石漠化区3种典型植被恢复模式(草地、林草地和林灌草地)下的土壤为研究对象,分别用干筛法和湿筛法研究不同年份、不同处理方法下不同土层的土壤团聚体稳定性和土壤有机碳储量的分布特征。结果表明:在林灌草地的两个土层土壤中平均重量直径(D_(mw))和几何平均直径(D_(gm))均高于草地和林草地;与2018年的结果相比,2019年各样地的土壤有机碳储量均升高,其中,与草地和林草地相比,林灌草地模式下的土壤有机碳储量显著升高(P<0.05),林灌草地土壤有机碳储量在0~10和10~20 cm土层分别比草地升高了52.52%和37.08%;与湿筛法的结果相比,干筛法能够更好地避免水溶性碳的损失,是表征喀斯特高原山地土壤团聚体稳定性的优选方法。混合种植的植被恢复模式能够提高土壤团聚体的稳定性,促进土壤有机碳的固存,有效维持喀斯特地区生态恢复的土壤质量,具有提高土壤养分储量的巨大潜力。

关键词：喀斯特土壤团聚体稳定性土壤有机碳储量高原山地植被恢复