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Oligomeric proanthocyanidin protects retinal ganglion cells against oxidative stress-induced apoptosis

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Neural Regeneration Research 2013年第25期8卷 2317-2326页

作者： Hui Wang Chanjuan Zhang Dan lu Xiaoming Shu Lihong Zhu Renbing Qi Kwok-Fai So daxiang lu Ying Xu Department of Pathophysiology Key Laboratory of State Administration of Traditional Chinese Medicine of ChinaJinan University School of Medicine GHM Institute of CNS Regeneration Jinan University Department of Pathophysiology Key Laboratory of State Administration of Traditional Chinese Medicine of ChinaJinan University School of Medicine Department of Pathophysiology Key Laboratory of State Administration of Traditional Chinese Medicine of China Jinan University School of Medicine

The death of retinal ganglion cells is a hallmark of many optic neurodegenerative diseases such as glaucoma and retinopathy. Oxidative stress is one of the major reasons to cause the cell death. Oligomeric proanthocyanidin has many health beneficial effects including antioxidative and neuro- protective actions. Here we tested whether oligomeric proanthocyanidin may protect retinal gan- glion cells against oxidative stress induced-apoptosis in vitro. Retinal ganglion cells were treated with hydrogen peroxide with or without oligomeric proanthocyanidin. 3-（4,5-Dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay showed that treating retinal ganglion cell line RGC-5 cells with 20 pmol/L oligomeric proanthocyanidin significantly decreased the hydrogen peroxide （H202） induced death. Results of flow cytometry and Hoechst staining demonstrated that the death of RGC-5 cells was mainly caused by cell apoptosis. We further found that expression of pro-apoptotic Bax and caspase-3 were significantly decreased while anti-apoptotic Bcl-2 was greatly increased in H202 damaged RGC-5 cells with oligomeric proanthocyanidin by western blot assay. Furthermore, in retinal explant culture, the number of surviving retinal ganglion cells in H202-damaged retinal ganglion cells with oligomeric proanthocyanidin was significantly increased. Our studies thus demonstrate that oligomeric proanthocyanidin can protect oxidative stress-injured retinal ganglion cells by inhibiting apoptotic process.

关键词： neural regeneration traditional Chinese medicineoxidative stress hydrogen peroxide retinalneuroregenerationretinal ganglion cell oligomeric proanthocyanidinexplants apoptosis grants-supported paper

luteolin protects PC12 cells from rotenone-induced apoptosis

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Neural Regeneration Research 2010年第24期5卷 1870-1874页

作者： Lihong Zhu Renbin Qi Huadong Wang Wei Bi Zhigang Wang Qi Zeng Yanru Zhao daxiang lu Department of Pathophyeiology Medical College of Jinan University Guangzhou 510632 Guangdong Province China Institute of Brain Research Jinan University Guangzhou 510632 Guangdong Province China Department of Neurology Sun Yat-sen Memorial Hospital of Sun Yat-sen University Guangzhou 510120 Guangdong Province China

The 4-hydroxy-flavonoid compound luteolin is a phytoestrogen with antioxidant, anti-inflammatory, and anti-tumor properties. The present study analyzed the protective effects of luteolin against rotenone-induced PC12 cell apoptosis in a model of Parkinson＇s disease （PD）. Rotenone （1.6 μmol/L） was utilized to establish the In vitro PD model, resulting in low cell viability and an apoptotic rate of 36.1%. luteolin （100 μmol/L） pretreatment significantly ameliorated rotenone-induced damage, improved cell morphology and viability, decreased comet tail length, reduced the rate of apoptosis （11.8%）, and down-regulated cleaved caspase-3 expression. Results demonstrate that luteolin protects PC12 cells against rotenone-induced apoptosis by decreasing cleaved caspase-3 expression.

关键词： apoptosis cleaved caspase-3 luteolin PC12 cells Parkinson's disease rotenone

α7烟碱受体拮抗剂对Aβ蛋白诱导损伤的PC12细胞保护作用的研究

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中国神经精神疾病杂志 2011年第9期37卷 540-544页

作者：汪志刚戚仁斌李卫沈文娟张晶赵彦儒朱丽红陆大祥暨南大学附属第一医院危重病医学科广州510630 暨南大学医学院病理生理学系国家中医药管理局病理生理重点实验室暨南大学附属第一医院神经外科

目的观察α7烟碱受体拮抗剂对Aβ蛋白诱导损伤的PC12细胞远期影响,探讨其细胞保护的作用。方法采用高分化的PC12细胞为研究对象,以Aβ25-35制作细胞损伤模型,以烟碱受体激动剂(尼古丁)和α7烟碱受体拮抗剂(甲基牛扁亭碱)进行预处理。试... 详细信息

目的观察α7烟碱受体拮抗剂对Aβ蛋白诱导损伤的PC12细胞远期影响,探讨其细胞保护的作用。方法采用高分化的PC12细胞为研究对象,以Aβ25-35制作细胞损伤模型,以烟碱受体激动剂(尼古丁)和α7烟碱受体拮抗剂(甲基牛扁亭碱)进行预处理。试验分为4组,分别是:对照组(蒸馏水)、模型组(Aβ25-35)、尼古丁组(尼古丁和Aβ25-35)、甲基牛扁亭碱组(甲基牛扁亭碱和Aβ25-35)。通过MTT比色法在多个时间点观察药物对细胞活力影响的动态变化,在此基础上采用流式细胞仪检测药物对细胞凋亡和坏死的影响。结果在所有时间点,模型组的细胞活力均显著低于对照组,凋亡和坏死率均高于对照组(P<0.01)。药物作用36～60h,尼古丁组的细胞活力显著高于模型组和甲基牛扁亭碱组(P<0.05),凋亡和坏死率较低(P<0.01);甲基牛扁亭碱组细胞活力及凋亡和坏死率与模型组差异无统计学意义。在药物作用84h后,与模型组和尼古丁组相比,甲基牛扁亭碱组细胞活力显著升高,凋亡和坏死率显著降低(P<0.01)。结论随着作用时间的延长(84h),烟碱受体激动剂(尼古丁)对PC12细胞的保护作用逐渐消失,而α7烟碱拮抗剂(甲基牛扁亭碱)逐渐显现出细胞保护作用。

关键词： β淀粉样蛋白 PC12细胞 α7烟碱受体拮抗剂烟碱受体激动剂细胞活力凋亡

油菜撒播轻型栽培技术研究 Ⅰ.油菜不同种植方式的产量及经济效益分析

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贵州农业科学 2008年第2期36卷 22-24,27页

作者：侯国佐侯燕侯剑伍贻春路大祥贵州省油菜研究所贵州贵阳550008 贵州省安顺市西秀区农业技术推广站贵州安顺561000

在中等施肥水平（纯N 7.36 kg/667m2）下采用油菜育苗移栽、开行点播、条播和撒播,667m2相应密度分别为4 167株、1万株、2.2-2.4万株和2.3-2.5万株的情况下,贵州思南塘头和安顺兴隆两地试验油研10号产量水平分别为165.0-175.0 kg/667m2... 详细信息

在中等施肥水平（纯N 7.36 kg/667m2）下采用油菜育苗移栽、开行点播、条播和撒播,667m2相应密度分别为4 167株、1万株、2.2-2.4万株和2.3-2.5万株的情况下,贵州思南塘头和安顺兴隆两地试验油研10号产量水平分别为165.0-175.0 kg/667m2和208.1-232.4 kg/667m2,产量间差异不显著,但点播、条播和撒播比育苗移栽用工减少7.29-10.56个/667m2,总投入减少130.67-173.72元/667m2,单位面积纯经济效益比育苗移栽高79.27-151.32元/667m2。单位劳动生产率比育苗移栽高16.88-30.45元/个工。点播、条播和撒播经济效益显著。

关键词：种植方式产量投入产出经济效益

油菜撒播轻型栽培技术研究 Ⅳ.撒播油菜单产160～220kg/667m^2的群体产量结构及栽培措施分析

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贵州农业科学 2008年第2期36卷 30-32页

作者：侯国佐侯剑侯燕伍贻春路大祥贵州省油菜研究所贵州贵阳550008 贵州省安顺市西秀区农业技术推广站贵州安顺561000

撒播油菜单产达到160~220 kg/667m2,应使667m2有效角在440~500万角以上、角粒数12~13粒、千粒重3.3~3.5 g。栽培上要留苗2.5~3万株/667m2,并施纯氮10~15 kg/667m2。

关键词：撒播产量产量结构群体结构轻型栽培技术

油菜撒播轻型栽培技术研究 Ⅲ.低肥水平下撒播油菜播种量与播种条件对产量的影响

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贵州农业科学 2008年第2期36卷 28-29,32页

作者：侯国佐侯剑侯燕伍贻春路大祥贵州省油菜研究所贵州贵阳550008 贵州省安顺市西秀区农业技术推广站贵州安顺561000

在低施肥水平下,试验研究了不同播种量和播种条件对撒播油菜出苗和产量的影响。结果表明,撒播油菜雨后抢墒（湿）即时播种,播后浅中耕使籽粒落入土中,油菜出苗快、出苗整齐、出苗率高;在低施肥（纯氮6.9 kg/667m2）水平下,雨后667m2播... 详细信息

在低施肥水平下,试验研究了不同播种量和播种条件对撒播油菜出苗和产量的影响。结果表明,撒播油菜雨后抢墒（湿）即时播种,播后浅中耕使籽粒落入土中,油菜出苗快、出苗整齐、出苗率高;在低施肥（纯氮6.9 kg/667m2）水平下,雨后667m2播种量为400 g,可成苗3万株左右,产量达170-180 kg/667m2。

关键词：撒播播种量播种条件密度

油菜撒播轻型栽培技术研究 Ⅵ.油菜撒播轻型栽培技术的大面积示范

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贵州农业科学 2008年第2期36卷 36-37,39页

作者：侯国佐侯剑侯燕伍贻春路大祥贵州省油菜研究所贵州贵阳550008 贵州省安顺市西秀区农业技术推广站贵州安顺561000

2003-2005年度在贵州省安顺市西秀区七眼桥镇用油研7号、油研10号、油研8号等进行油菜撒播高产、高效的轻型配套栽培技术试验示范,面积分别为3.41 hm2和100 hm2,平均单产分别达164.1 kg/667m2和133.2 kg/667m2。其中,2004年验收的10块... 详细信息

2003-2005年度在贵州省安顺市西秀区七眼桥镇用油研7号、油研10号、油研8号等进行油菜撒播高产、高效的轻型配套栽培技术试验示范,面积分别为3.41 hm2和100 hm2,平均单产分别达164.1 kg/667m2和133.2 kg/667m2。其中,2004年验收的10块地（测量面积合计0.685 hm2）的撒播油菜平均产量为158.9 kg/667m2,最高产量达210-230 kg/667m2;2005年验收各类田块19块（面积合计1.159 hm2）,单产105-206.3 kg/667m2。除去人工（每个工按15元计）、牛工（每个工按30元计）、物资投入外,667m2纯收入达189.57元,单个劳动力的产值达35.83元,比同地育苗移栽分别高131.11元/667m2和17.83元/个,比当地传统的苦油菜（芥菜型）撒播栽培增收175.72元/667m2。

关键词：撒播油菜高产轻型栽培技术示范纯收入

Assembly of Nanowires into Macroscopic One‑Dimensional Fibers in Liquid State

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Advanced Fiber Materials 2023年第3期5卷 928-954页

作者： Wentao Cao Xinyu Zhao Bingqiang lu daxiang Cui Feng Chen Center for Orthopaedic Science and Translational Medicine Department of OrthopaedicsShanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200072People’s Republic of China National Engineering Research Center for Nanotechnology Shanghai 200241People’s Republic of China Institute of Micro‑Nano Science and Technology School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Natural structural materials,such as spider silk,wood,and bone,are universally acknowledged as the gold standard for the ideal combinations of strength and *** exceptional integrated performance of these biological materials can be ascribed to their multiscale hierarchical architectures and *** the hierarchical assembly feature of natural materials,artificial fibers,which are generated through the one-dimensional(1D)assembly of nanowires,have been widely reported with remarkable flexibility and ***,the distinguishing feature of nanowires’1D assembly can bridge the unique properties of nanowires with their potential functional *** tutorial review summarizes the recent developments in the assembly of nanowires into macroscopic 1D fibers in the liquid *** begin by introducing the general strategies and mechanisms for assembling nanowires in one direction and then,illustrate their potential applications in energy storage,sensors,biomedical engineering,***,a brief summary and some personal perspectives on the future research directions of nanowires’1D assembly are also proposed.

关键词： One-dimensional assembly Nanowires Fibers Liquid state

R11 peptides can promote the molecular imaging of spherical nucleic acids for bladder cancer margin identification

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Nano Research 2022年第3期15卷 2278-2287页

作者： Minghai Ma Pu Zhang Xiao Liang daxiang Cui Qiuya Shao Haibao Zhang Mengzhao Zhang Tao Yang lu Wang Nan Zhang Minxuan Jing lu Zhang Weichao Dan Rundong Song Xi Liu Jiatao Hao Yuhang Chen Lijiang Gu Lei Wang Jinhai Fan Department of Urology The First Affiliated HospitalXi’an Jiaotong UniversityXi’an 710061China Department of Urology Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou 310014China Department of Thoracic Surgery Tangdu HospitalAir Force Medical UniversityXi’an 710038China Department of Bio-Nano-Science and Engineering National Key Laboratory of Nano/Micro Fabrication TechnologyInstitute of Micro-Nano Science and TechnologyShanghai Jiao Tong UniversityShanghai 200240China

One of the critical problems in bladder cancer(BC)management is the local recurrence of ***,achieving the accurate delineation of tumor margins intraoperatively remains extremely difficult due to the lack of effective tumor margin recognition ***,survivin molecular beacon(MB)and R11 peptide-linked spherical nucleic acids(SNAs)were synthesized as nanoprobes(AuNP-MB@R11)for sensitive detection of BC *** properties proved that R11 peptides and survivin MB were successfully loaded onto the surface of ***-MB@R11 had good stability against nuclease activity and high sensitivity and specificity to detect survivin single strand DNA(ssDNA)in *** to cytology,R11 peptides could increase the BC targeting ability and membrane penetrability of ***,R11 peptides significantly promoted the disintegration of lysosomes and the release of SNAs to enhance fluorescence imaging *** RNA sequencing proved that some genes and pathways related to endocytosis and lysosomes were significantly regulated,such as AGPAT5,GPD1L,and *** orthotopic BC models and a clinical sample from a patient with BC,AuNP-MB@R11 showed a more legible cancerous fluorescence margin and offered remarkably improved detection compared to those achieved by SNAs.R11 peptide-linked SNAs present promising potential to identify BC margin,which may help to improve the R0 resection rate in surgery and improve patients’quality of life.

关键词： R11 peptides molecular beacons spherical nucleic acids tumor targeting lysosome escape bladder cancer