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Molecular Plant 2021年第10期14卷 1771-1771页

作者： Felipo-Benavent Lucia Colombo Miguel A.blázquez david Alabadi Simona Masiero 不详

(Molecular Plant8(3):482–485;March 2015;https://***/10.1016/***.2014.11.018)The supplemental figure S3(panel A)was amended since the picture of the yeast control spot TCP14-AD/M5rGL2BD(-W-L medium)has been used in place of TCP15-AD/*** authors apologize for not detecting this error prior to publication and for any inconvenience that may have caused.

关键词： TCP1 figure Arabidopsis

PCr-HrM for Genomic Surveillance of SArS-CoV-2: A Variant Detection Tool in Côte d’Ivoire, West Africa

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American Journal of Molecular Biology 2024年第3期14卷 166-185页

作者： Aboubacar Sylla Solange Kakou-Ngazoa Tata Gniré Safiatou Coulibaly Yakoura Karidja Ouattara Mireille Sylvie Kouamé-Sina Zeinab Ouattara david Ngolo Coulibaly Brice Kouakou bla Mireille Dosso Molecular Biology Platform Pasteur Institute Cte dIvoire Abidjan Cte dIvoire Laboratory of Biology and Health UFR Biosciences University Flix Houphout Boigny Abidjan Cte dIvoire Genetic and Cancer Laboratory Molecular Genetics Platform Pasteur Institute Cte dIvoire Abidjan Cte dIvoire Epidemic Virus Department Pasteur Institute Cte dIvoire Abidjan Cte dIvoire

The rise of new viruses, like SArS-CoV-2 causing the COVID-19 outbreak, along with the return of antibiotic resistance in harmful bacteria, demands a swift and efficient reaction to safeguard the health and welfare of the global population. It is crucial to have effective measures for prevention, intervention, and monitoring in place to address these evolving and recurring risks, ensuring public health and international security. In countries with limited resources, utilizing recombinant mutation plasmid technology in conjunction with PCr-HrM could help differentiate the existence of novel variants. cDNA synthesis was carried out on 8 nasopharyngeal samples following viral rNA extraction. The P1 segment of the SArS-CoV-2 Spike S protein was amplified via conventional PCr. Subsequently, PCr products were ligated with the pGEM-T Easy vector to generate eight recombinant SArS-CoV-2 plasmids. Clones containing mutations were sequenced using Sanger sequencing and analyzed through PCr-HrM. The P1 segment of the S gene from SArS-CoV-2 was successfully amplified, resulting in 8 recombinant plasmids generated from the 231 bp fragment. PCr-HrM analysis of these recombinant plasmids differentiated three variations within the SArS-CoV-2 plasmid population, each displaying distinct melting temperatures. Sanger sequencing identified mutations A112C, G113T, A114G, G214T, and G216C on the P1 segment, validating the PCr-HrM findings of the variations. These mutations led to the detection of L452r or L452M and F486V protein mutations within the protein sequence of the Omicron variant of SArS-CoV-2. In summary, PCr-HrM is a vital and affordable tool for distinguishing SArS-CoV-2 variants utilizing recombinant plasmids as controls.

关键词： Genomic Surveillance SArS-CoV-2 PCr-HrM Variants Côte d’Ivoire

Identification of novel molecular markers of mastitis caused by Staphylococcus aureus using gene expression profiling in two consecutive generations of Chinese Holstein dairy cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 155-171页

作者： Di Wang Lei Liu Serafino M.A.Augustino Tao Duan Thomas J.Hall david E.MacHugh Jinhuan Dou Yi Zhang Yachun Wang Ying Yu Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture&National Engineering Laboratory for Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China UCD School of Agriculture and Food Science University College DublinDublin D04 V1W8Ireland Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Department of Animal Nutrition and Feed Science College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China UCD Conway Institute of Biomolecular and Biomedical research University College DublinDublin D04 V1W8Ireland

Background: Mastitis in dairy cows caused by Staphylococcus aureus is a major problem hindering economic growth in dairy farms worldwide. It is difficult to prevent or eliminate due to its asymptomatic nature and long persistence of infection. Although transcriptomic responses of bovine mammary gland cells to pathogens that cause mastitis have been studied, the common responses of peripheral blood leukocytes to S. aureus infection across two consecutive generations of dairy cattle have not been ***: In the current study, rNA-Seq was used to profile the transcriptomes of peripheral blood leukocytes sampled from S. aureus-infected mothers and their S. aureus-infected daughters, and also healthy non-infected mothers and their healthy daughters. Differential gene expression was evaluated as follows: 1) S. aureus-infected cows versus healthy non-infected cows(S vs. H, which include all the mothers and daughters), 2) S. aureus-infected mothers versus healthy non-infected mothers(SM vs. HM), and 3) S. aureus-infected daughters versus healthy noninfected daughters(SMD vs. HMD).results: Analysis of all identified expressed genes in the four groups(SM, SMD, HM, and HMD) showed that EPOr,IL9, IFNL3, CCL26, IL26 were exclusively expressed in both the HM and HMD groups, and that they were significantly(P riched for the cytokine-cytokine receptor interaction pathway. A total of 17, 13 and 10 differentially expressed genes(DEGs)(FDr Padj. 1.2) were detected in the three comparisons, respectively. DEGs with P 2 were used for functional enrichment analyses. For the S vs. H comparison, DEGs detected included CCL20, IL13 and MMP3, which are associated with the IL-17 signaling pathway. In the SM vs. HM and SMD vs. HMD comparisons, five(bla-DQB, C1 r, C2, FCGr1 A, and KrT10) and six(bla-DQB, C3 Ar1, CFI, FCAr, FCGr3 A, and LOC10498484) genes, respectively, were involved in the S. aureus infection ***: Our stud

关键词： Dairy cow Disease resistance Mastitis Peripheral blood leukocyte Staphylococcus aureus Transcriptome Two generations

Biodegradable magnesium barrier membrane used for guided bone regeneration in dental surgery

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Bioactive Materials 2022年第8期7卷 152-168页

作者： Patrick rider Zeljka Peric Kacarevic Akiva Elad Drazen Tadic Daniel rothamel Gerrit Sauer Fabien Bornert Peter Windisch david Botond Hangyasi Balint Molnar Emely Bortel Bernhard Hesse Frank Witte Department of Prosthodontics Geriatric Dentistry and Craniomandibular DisordersCharite-Universitatsmedizin BerlinAßmannshauser Straße 4-614197BerlinGermany Botiss Biomaterials AG Ullsteinstrasse 10812109BerlinGermany Department of Anatomy Histology EmbryologyPathology Anatomy and Pathology HistologyFaculty of Dental Medicine and HealthUniversity of OsijekOsijek31000Croatia CMF Surgery Johannes BLA HospitalMonchengladbachGermany Dental Clinic University of StrasbourgFrance Department of Periodontology Semmelweis UniversityBudapestHungary Xploraytion GmbH Bismarkstrasse 11BerlinGermany Biotrics Bioimplants AG Ullsteinstrasse 10812109BerlinGermany

Barrier membranes are commonly used as part of the dental surgical technique guided bone regeneration(GBr)and are often made of resorbable collagen or non-resorbable materials such as *** collagen membranes do not provide sufficient mechanical protection of the covered bone defect,titanium reinforced membranes and non-resorbable membranes need to be removed in a second ***,biodegradable GBr membranes made of pure magnesium might be an *** this study a biodegradable pure magnesium(99.95%)membrane has been proven to have all of the necessary requirements for an optimal regenerative outcome from both a mechanical and biological *** implantation,the magnesium membrane separates the regenerating bone from the overlying,faster proliferating soft *** the initial healing period,the membrane maintained a barrier function and space provision,whilst retaining the positioning of the bone graft material within the defect *** the magnesium metal corroded,it formed a salty corrosion layer and local gas cavities,both of which extended the functional lifespan of the membrane barrier *** the resorption of the magnesium metal and magnesium salts,it was observed that the membrane became surrounded and then replaced by new *** the membrane had completely resorbed,only healthy tissue *** in vivo performance study demonstrated that the magnesium membrane has a comparable healing response and tissue regeneration to that of a resorbable collagen ***,the magnesium membrane demonstrated all of the ideal qualities for a barrier membrane used in GBr treatment.

关键词： Magnesium Biodegradable Implant GBr Bone healing Soft tissue healing

Biodegradable magnesium fixation screw for barrier membranes used in guided bone regeneration

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Bioactive Materials 2022年第8期7卷 15-30页

作者： Zeljka Peric Kacarevic Patrick rider Akiva Elad Drazen Tadic Daniel rothamel Gerrit Sauer Fabien Bornert Peter Windisch david Botond Hangyasi Balint Molnar Till Kammerer Bernhard Hesse Emely Bortel Marco Bartosch Frank Witte Department of Anatomy Histology EmbryologyPathology Anatomy and Pathology HistologyFaculty of Dental Medicine and HealthUniversity of OsijekOsijek31000Croatia Department of Prosthodontics Geriatric Dentistry and Craniomandibular DisordersCharite-Universitatsmedizin BerlinAßmannshauser Straße 4–614197BerlinGermany Botiss Biomaterials AG Ullsteinstrasse 10812109BerlinGermany CMF Surgery Johannes BLA HospitalMonchengladbachGermany Dental Clinic University of StrasbourgFrance Department of Periodontology Semmelweis UniversityBudapestHungary Xploraytion GmbH Bismarkstrasse 11BerlinGermany University Hospital Munich Department of Dermatology and AllergyFrauenlobstr.9-1180337MunichGermany Biotrics Bioimplants AG Ullsteinstrasse 10812109BerlinGermany

An ideal fixation system for guided bone(GBr)regeneration in oral surgery must fulfil several criteria that includes the provision of adequate mechanical fixation,complete resorption when no longer needed,complete replacement by bone,as well as be biocompatible and have a good clinical *** the first time,a biodegradable magnesium fixation screw made of the magnesium alloy WZM211 with a MgF2 coating has been designed and tested to fulfill these *** mechanical fixation was shown for the magnesium fixation screw in several benchtop tests that directly compared the magnesium fixation screw with an equivalent polymeric resorbable *** demonstrated slightly superior mechanical properties of the magnesium device in comparison to the polymeric device even after 4 weeks of *** of the magnesium fixation screw was demonstrated in several in vitro and in vivo *** of the magnesium screw was investigated in in vitro and in vivo tests,where it was found that the screw is resorbed slowly and completely after 52 weeks,providing adequate fixation in the early critical healing ***,the magnesium fixation screw demonstrates all of the key properties required for an ideal fixation screw of membranes used in guided bone regeneration(GBr)surgeries.

关键词： Magnesium Biodegradable Implant GBr Bone healing Soft tissue healing