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Burn injury induces histopathological changes and cellproliferation in liver of rats

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World Journal of Hepatology 2016年第6期8卷 322-330页

作者： Jeferson Andre Bortolin Hananiah Tardivo Quintana Tabata de carvalho Tome Flavia Andressa Pidone Ribeiro daniel Araki Ribeiro Flavia de Oliveira department of Biosciences Federal University of São Paulo(UNIFESP)SantosSP 11060-001Brazil

AIM: To investigate effects of severe burn injury(BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control(C) and subjected to scald BI(SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative(hepatocyte area and cell density), ciclooxigenase-2(COX-2) immunoexpression, and gene expression [real-time polymerase chain reaction for tumor necrosis factor(TNF)-α, inducible nitric oxide synthase(i NOS) and caspase-3] methods. RESULTS: Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-α, i NOS and caspase-3 gene expression. CONCLUSION: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.

关键词： Burn injury Morphology Histophatology Liver Ciclooxigenase-2

Special Issue on 15th Brazilian Polymer Conference:Biopolymers,Eco-Friendly and Biodegradable Polymers and Other Topics Related to Polymeric Materials derived from Renewable Materials

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Journal of Renewable Materials 2021年第4期9卷 599-600页

作者： Antonio Aprigio da Silva Curvelo Antonio José Felix carvalho daniel Pasquini São Carlos Institute of Chemistry University of São PauloSão Carlos13566-590-SPBrazil São Carlos School of Engineering University of São PauloSão Carlos13563-120-SPBrazil Institute of Chemistry Federal University of UberlândiaUberlândia38400-902MGBrazil

This issue of Journal of Renewable Materials is a special issue dedicated to the 15th Brazilian Polymer Conference(15th CBPol)which was held in Bento Gonçalves,Rio Grande do Sul State,Brazil,from 27 to 31 October,2019[1].CBPol is the main scientific forum in the field of polymer science and technology in *** conference has been held biannually since 1991 and in this event had almost 900 participants mainly from Brazil,but also from Argentina,Chile,Peru,USA,Canada,Belgium,France,Great Britain,Portugal and Spain,with 60%students and 40%professionals.A total of 1,100 papers from 2725 authors were presented,from which 25%(280 papers)was in the field of Biomaterials showing the importance of these materials which are in general based on renewable *** 280 works were developed mainly by researchers from Brazilian institutions(universities and research institutes),mainly from the south and southeast regions of the *** subject of more than 70%of the works was dedicated on vegetal raw materials,medicinal applications,packaging and composite materials.

关键词： materials showing annually

Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures

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Journal of Animal Science and Biotechnology 2018年第1期9卷 208-216页

作者： Aline Gomes da Silva Mário fonseca Paulino Edenio detmann Henrique Jorge Fernandes Lincoln da Silva Amorim Román Enrique Maza Ortega Victor Valério de carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de Moura Mariana Benevides Monteiro Jéssika Almeida Bitencourt Universidade Federal de Vicosa VicosaMinas Gerais 36570-000Brazil Universidade Federal de Mato Grosso do Sul Campo GrandeMato Grosso do Sul 79074-460Brazil Universidade Estadual de Mato Grosso do Sul AquidauanaMato Grosso do Sul 79200-000Brazil Biotran Biotecnologia e Treinamento em Reproducao Animal AlfenasMinas Gerais 37130-000Brazil Universidade Federal do Acre Rio BrancoAcre 69920-900Brazil

Background： Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments： control, which received no supplement; supplementation for the last 30 d of gestation（30-d; 3.0 kg/d）; supplementation for the last 60 d of gestation（60-d; 1.5 kg/d）; or supplementation for the last 90 d of gestation（90-d; 1.0 kg/d）. All supplemented treatments received the same total amount of supplement throughout the experiment： 90 kg（20% of crude protein）. A second experiment（Experiment 2） was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d ***： There was a linear effect of the number of days of supplementation on calving body weight（BW; P 〈 0.05）and a quadratic effect on BW change from parturition to d 31 post-calving（P 〈 0.05）, with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW（P 〉 0.10）. A significant linear effect on interval from parturition to conception（P 〈 0.05） was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility（P 〉 0.10）. Nitrogen excreted through urine tended to increase linearly with the level of supplementation（P 〈 0.10）.Conclusion： Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of

关键词： Flushing Nutrition Parturition Reproduction

Increased inspiratory esophagogastric junction pressure in systemic sclerosis:An add-on to antireflux barrier

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World Journal of Gastroenterology 2015年第7期21卷 2067-2072页

作者： Miguel ngelo Nobre e Souza Patrícia carvalho Bezerra Rivianny Arrais Nobre Esther Studart da fonseca Holanda Armênio Aguiar dos Santos department of Clinical Medicine Federal University of Ceará department of Physiology and Pharmacology Federal University of Ceará

AIM:To investigate crural diaphragm(CD)function in systemic sclerosis(SSc)using high-resolution manometryand standardized inspiratory ***:Eight SSc volunteers(average age,40.1years;one male)and 13 controls(average age,32.2years;six males)participated in the study.A highresolution manometry/impedance system measured the esophagus and esophagogastric junction(EGJ)pressure profile during swallows and two respiratory maneuvers:sinus arrhythmia maneuver(SAM;the average of six EGJ peak pressures during 5-s deep inhalations)and threshold maneuver(TM;the EGJ peak pressures during forced inhalation under 12 and 24 cm H2O loads).Inspiratory diaphragm lowering(IDL)was taken as the displacement of the EGJ high-pressure zone during the ***:SSc patients had lower mean lower esophageal sphincter pressure than controls during normal breathing(19.7±2.8 mm Hg vs 32.2±2.7 mm Hg,P=0.007).Sinus arrhythmia maneuver pressure was higher in SSc patients than in controls(142.6±9.4 mm Hg vs 104.6±13.8 mm Hg,P=0.019).Sinus arrhythmia maneuver pressure normalized to IDL was also higher in SSc patients than in controls(83.8±13.4 mm Hg vs37.5±6.9 mm Hg,P=0.005).Threshold maneuver pressures normalized to IDL were also greater in SSc patients than in controls(TM 12 cm H2O:85.1±16.4mm Hg vs 43.9±6.3 mm Hg,P=0.039;TM 24 cm H2O:85.2±16.4 mm Hg vs 46.2±6.6 mm Hg,P=0.065).Inspiratory diaphragm lowering in SSc patients was less than in controls(2.1±0.3 cm vs 3±0.2 cm,P=0.011).CONCLUSION:SSc patients had increased inspiratory EGJ *** is an add-on to EGJ pressure and indicates that the antireflux barrier can be trained.

关键词： Systemic sclerosis Crural diaphragm Gastroesophage

Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 363-370页

作者： daniel Barroso de Alencar Fátima Cristiane Teles de carvalho Rosa Helena Rebouas daniel Rodrigues dos Santos Kelma Maria dos Santos Pires-Cavalcante Rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio Laboratrio de Produtos Naturais Marinhos.departamento de Engenharia de Pesca Universidade Federal do Ceará.Campus do Pici.Ar.Mister Hull.s/nCaixa Postal 6043 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará.Av.da Abolicao 3207 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará Laboratrio de Produtos Naturais Marinhos.departamento de Engenharia de Pesca Universidade Federal do Ceará Laboratrio de Cromatografia Departamento de QuimicaUniversidade do Estado do Rio Grande do NorteCampus Universitário Central

Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%EtOH) extracts of two species of red seaweeds Pterocladiella capillacea(***) and Osmundaria ***:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content *** activity was tested using the method of disc diffusion on Mueller-Hinton *** ability of algal extracts to agglutinate bacterial cells was also ***:The 70%EtOH extract of the two algae showed the highest values of total phenolic content compared to the Hex *** results of DPPH for both extracts(Hex,70%EtOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of ***(33.04%and 40.81%) at a concentration of 1 000 μg/*** for the ferrous ion chelating,there was an opposite behavior,extracts of *** had a higher *** extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to *** activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains ***,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of Escherichia coli,multidrug-resistant Salmonella and Vibrio *** This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells.

关键词： Bioactive compounds Antioxidant Bacterial agglutination Rhodophyta

The Performance of the School Psychologist in Schools for the Inclusion of Autistic Students in the Scenario of COVID-19

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Psychology Research 2023年第2期13卷 60-74页

作者： Vinicius Cutrim Ferreira Pollianna Galvão Soares de Matos daniel carvalho de Matos Francidalma Soares Sousa carvalho Filha Universidade Ceuma São LuísBrazil Instituto Evoluir São LuísBrazil Universidade Federal do Maranhão São LuísBrazil Universidade Estadual do Maranhão São LuísBrazil

During COVID-19,the repercussions on scholar dynamics,functioning and structure were inevitable,causing an impact on groups that,historically,are minority in relation to the scope of school inclusion policies,such as students diagnosed with autism spectrum disorder(ASD).This scenario is followed by educational phenomena in the face of which School Psychology has collaborated with the advancement of research and knowledge *** qualitative study,based on Historical-Cultural Psychology and Critical School Psychology,analyzed the role of the school psychologist in monitoring students diagnosed with ASD in the context of regular school during the *** following procedures were conducted:application of an online questionnaire;and semi-structured interviews with four school *** a result,three zones of shared meanings were built:the claim for the right to education with support and adaptations;the psychologist as the great savior of inclusion;the non-total inclusion that denounces the structure of injustice by *** information suggests that professional performance was hampered by different obstacles in the pandemic,such as the difficulty imposed by school,overload of demands,and impasses in the implementation of inclusion historically experienced at school,revealing that the school structure still carries historical consequences of *** shared meanings have demanded a professional performance with more effort,resistance,and strategies that accept the demands of school inclusion,providing collective mobilization,together with school actors,capable of breaking down barriers,especially those related to capacitist concepts.

关键词： school psychology autism spectrum disorder school inclusion pandemic

First Serological Evidence on Endemicity of HEV Infection in Wild Boar(Sus scrofa) Populations from Portugal

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Virologica Sinica 2018年第2期33卷 197-200页

作者： daniel Goncalves Joao Pereira-Vaz Vitor Duque Victor Bandeira Carlos fonseca Ana Donato Cristina Luxo Ana Miguel Matos Laboratory of Virology Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Laboratory of Molecular Biology Clinical Pathology UnitHospital and University Centre of Coimbra3000-602 Coimbra Portugal Infectious Diseases department Hospital and University Centre of Coimbra 3000-075 Coimbra Portugal Biology department and CESAM University of Aveiro3810-193 Aveiro Portugal Clinical Laboratory Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Research Centre on Chemical Processes Engineering and Forest Products (CIEPQF) Faculty of Pharmacy University of Coimbra 3030-790 Coimbra Portugal

Increasing number of hepatitis E virus（HEV）infection has been described in industrialized countries,with infection occurring as a sporadic disease,usually associated with travel to endemic regions or small outbreaks... 详细信息

关键词：感染 HEV 血清学葡萄牙野猪证据特性人口

Analysis of Soft decision Trees for Passive-Expert Reinforcement Learning

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American Journal of Computational Mathematics 2022年第2期12卷 209-215页

作者： Jonathan Martini daniel J. fonseca department of Mechanical Engineering The University of Alabama Tuscaloosa USA

This paper explores the use of soft decision trees [1] in basic reinforcement applications to examine the efficacy of using passive-expert like networks for optimal Q-Value learning on Artificial Neural Networks (ANN). The soft decision tree networks were built using the PyTorch machine learning and the OpenAi’s Gym environment frameworks. The conducted research study aimed at assessing the performance of soft decision tree networks on Cartpole as provided in the OpenAi Gym software package. The baseline performance metric that the soft decision tree networks were compared against was a simple deep Neural Network using several linear layers with ReLU and Softmax activation functions for the input and output layers, respectively. All networks were trained using the Backpropagation algorithm provided generically by PyTorch’sAutograd module.

关键词： deep Learning Soft decision Trees Passive Reinforcement Learning Recurrent Neural Networks

Encephalic hemodynamic phases in subarachnoid hemorrhage:how to improve the protective effect in patient prognoses

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Neural Regeneration Research 2015年第5期10卷 748-752页

作者： Marcelo de Lima Oliveira daniel Silva de Azevedo Milena Krajnyk de Azevedo Ricardo de carvalho Nogueira Manoel Jacobsen Teixeira Edson Bor-Seng-Shu Division of Neurological Surgery Hospital das ClinicasSchool of MedicineUniversity of Sao Paulo

Subarachnoid hemorrhage is frequently associated with poor prognoses. Three different hemo- dynamic phases were identified during subarachnoid hemorrhage： oligemia, hyperemia, and vasospasm. Each phase is associated with brain metabolic changes. In this review, we correlated the hemodynamic phases with brain metabolism and potential treatment options in the hopes of improving patient prognoses.

关键词： hemodynamic phases cerebral subarachnoid hemorrhage metabolic crises