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压电陶瓷执行器的神经网络实时自适应逆控制

光学精密工程 2008年第7期16卷 1266-1272页

作者：党选举桂林电子科技大学智能系统与工业控制研究室广西桂林541004

提出了基于内积的压电陶瓷动态神经网络非线性、非光滑迟滞逆模型,采用反馈误差学习方法,快速地在线得到压电陶瓷的逆模型,避免了通过正模型求取压电陶瓷的Jacobian信息。结合PID反馈控制,在dSPACE系统平台上实现了压电陶瓷的神经网络... 详细信息

提出了基于内积的压电陶瓷动态神经网络非线性、非光滑迟滞逆模型,采用反馈误差学习方法,快速地在线得到压电陶瓷的逆模型,避免了通过正模型求取压电陶瓷的Jacobian信息。结合PID反馈控制,在dSPACE系统平台上实现了压电陶瓷的神经网络自适应逆控制。为提高实时性,采用了效率高、速度快的C-MEX S Function编程。实验结果表明:神经网络自适应逆控制的控制精度为0.13μm,而PID控制精度为0.32μm。所提出方法有效地消除了迟滞的影响,控制精度高。

关键词：匪电陶瓷迟滞特性神经网络自适应逆控制 dSPACE系统非光滑迟滞逆模型

Investigation of gas content of organic-rich shale:A case study from Lower Permian shale in southern North China basin,central China

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Geoscience Frontiers 2018年第2期9卷 559-575页

作者： Wei dang Jin-Chuan Zhang xuan Tang Xiao-Liang Wei Zhong-Ming Li Cheng-Hu Wang Qian Chen Chong Liu School of Energy Resources China University of GeosciencesBeijing 100083China Key Laboratory of Shale Gas Exploration and Evaluation Ministry of Land and ResourcesChina University of GeosciencesBeijing 100083China Henan Institute of Geological Survey Zhengzhou 450000China Key Laboratory of Crustal Dynamics Institute of Crustal DynamicsChina Earthquake AdministrationBeijing 100085China

Measuring gas content is an essential step in estimating the commerciality of gas reserves. In this study,eight shale core samples from the Mouye-1 well were measured using a homemade patented gas desorption apparatus to determine their gas contents. Due to the air contamination that is introduced into the desorption canister, a mathematical method was devised to correct the gas quantity and *** to the chemical compositions of desorbed gas, the chemical compositions of residual gas are somewhat different. In residual gas, carbon dioxide and nitrogen record a slight increase, and propane is first observed. This phenomenon may be related to the exposure time during the transportation of shale samples from the drilling site to the laboratory, as well as the differences in the mass, size and adsorptivity of different gas molecules. In addition to a series of conventional methods, including the USBM direct method and the Amoco Curve Fit(ACF) method, which were used here for lost gas content estimation, a Modified Curve Fit(MCF) method, based on the 'bidisperse' diffusion model, was established to estimate lost gas content. By fitting the ACF and MCF models to gas desorption data, we determined that the MCF method could reasonably describe the gas desorption data over the entire time period, whereas the ACF method failed. The failure of the ACF method to describe the gas desorption process may be related to its restrictive assumption of a single pore size within shale samples. In comparison to the indirect method, this study demonstrates that none of the three methods studied in this investigation(USBM, ACF and MCF) could individually estimate the lost gas contents of all shale samples and that the proportion of free gas relative to total gas has a significant effect on the estimation accuracy of the selected method. When the ratio of free gas to total gas is lower than 45%, the USBM method is the best for estimating the lost gas content, whereas when the rat

关键词： Organic-rich shale Gas content Air contamination Modified curve fit method Lost gas content estimation

Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress

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International Journal of Oral Science 2022年第4期14卷 479-488页

作者： Bingdong Sui Jin Liu Chenxi Zheng Lei dang Ji Chen Yuan Cao Kaichao Zhang Lu Liu Minyan dang Liqiang Zhang Nan Chen Tao He Kun xuan Fang Jin Ge Zhang Yan Jin Chenghu Hu State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi International Joint Research Center for Oral Diseases Center for Tissue EngineeringSchool of StomatologyThe Fourth Military Medical UniversityXi'anChina Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases School of Chinese MedicineHong Kong Baptist UniversityHong Kong SARChina Xi'an Key Laboratory of Stem Cell and Regenerative Medicine Institute of Medical ResearchNorthwestern Polytechnical UniversityXianChina Xi'an Institute of Tssue Engineering and Regenerative Medicine XianChina Department of Biochemistry and Molecular Biology School of Basic Medical SciencesXi'an Jiaotong UniversityXi'anChina

Sympathetic cues via the adrenergic signaling critically regulate bone homeostasis and contribute to neurostress-induced bone loss,but the mechanisms and therapeutics remain incompletely ***,we reveal an osteoclastogenesis-centered functionally important osteopenic pathogenesis under sympatho-adrenergic activation with characterized micro RNA response and efficient *** discovered that osteoclastic mi R-21 was tightly regulated by sympatho-adrenergic cues downstream theβ2-adrenergic receptor(β2AR)signaling,critically modulated osteoclastogenesis in vivo by inhibiting programmed cell death 4(Pdcd4),and mediated detrimental effects of both isoproterenol(ISO)and chronic variable stress(CVS)on ***,without affecting osteoblastic bone formation,bone protection against ISO and CVS was sufficiently achieved by a(D-Asp8)-lipid nanoparticle-mediated targeted inhibition of osteoclastic mi R-21 or by clinically relevant drugs to suppress ***,these results unravel a previously underdetermined molecular and functional paradigm that osteoclastogenesis crucially contributes to sympatho-adrenergic regulation of bone and establish multiple targeted therapeutic strategies to counteract osteopenias under stresses.

关键词： osteoclast pathogenesis homeostasis

Anti-inflammatory effects of Solanum procumbens on a low dose complete Freund's adjuvant-induced arthritis rat model

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Asian Pacific Journal of Tropical Biomedicine 2023年第5期13卷 214-221页

作者： xuan-Hai Do Trong Nghia Nguyen Thanh Chung dang Thi Thanh Mai Nguyen Trung Nhan Nguyen Van Nhat Truong Do Huu Tho Le xuan Hai Nguyen Hoang Phu dang Giang-Linh Nguyen Dinh-Khanh Hoang Van-Quan Le Van-Mao Can Department of Practical and Experimental Surgery Vietnam Military Medical UniversityHanoi 10000Vietnam Department of Reconstructive and Microsurgery Craniofacial and Reconstructive Surgery CentreMilitary Central Hospital 108Hanoi 10000Vietnam Department of Anatomic Pathology Military Hospital 103Vietnam Military Medical UniversityHanoi 10000Vietnam Faculty of Chemistry University of ScienceVietnam National University Ho Chi Minh CityHo Chi Minh City 70000Vietnam Faculty of Medicine University of Medicine and PharmacyVietnam National UniversityHanoi 10000Vietnam Department of Ultrasound Imaging Diagnostics CentreMilitary Hospital 103Vietnam Military Medical UniversityHanoi 10000Vietnam Department of Functional Diagnostics Military Hospital 103Vietnam Military Medical UniversityHanoi 10000Vietnam Department of Pathophysiology Vietnam Military Medical UniversityHanoi 10000Vietnam

Objective:To investigate the anti-inflammatory and analgesic effects of Solanum procumbens on complete Freund’s adjuvantinduced arthritis rat ***:We isolated and identified five compounds in the ethanolsoluble Solanum procumbens extract(SP)with anti-inflammatory effects,including ursolic acid,β-sitosterol,hexadecanoic acid,cisvaccenic acid,and vanillic ***,we investigated the anti-inflammatory effects of SP on rheumatoid arthritis symptoms,including paw volumes,local temperatures,withdrawal latency,and mechanical withdrawal threshold at the hind paw and white blood cell(WBC)number from complete Freund’s adjuvant-induced arthritis rat ***:We have successfully established a complete Freund’s adjuvant-induced arthritis rat model at a low dose(1 mg/mL).SP extract significantly reduced paw volumes(P<0.05),prolonged withdrawal latencies(P<0.05),decreased local temperature,and increased the mechanical withdrawal threshold(P<0.05),but only SP extract at the dose of 300 mg/kg significantly decreased WBC ***:SP extract could be a potential medication candidate with anti-inflammatory effects for arthritis,but it requires further investigation into the mechanism of the SP and its effectiveness on other models as well as clinical trials.

关键词： Solanum procumbens Arthritis Anti-inflammation Complete Freund's adjuvant Rats

Involvement of the Mitochondrion-dependent and the Endoplasmic Reticulum Stress-signaling Pathways in Isoliquiritigenin-induced Apoptosis of HeLa Cell

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Biomedical and Environmental Sciences 2013年第4期26卷 268-276页

作者： YUAN xuan ZHANG Bo GAN Lu WANG Zhen Hua YU ba Cui LIU Liang Liang ZHENG Qiu Sheng WANGZhi Ping Lanzhou University Second Hospital Lanzhou University Key Laboratory of Xinjiang Endemic Phytomedicine Resources Ministry of EducationSchool of PharmacyShihezi University Institute of Modern Physics Chinese Academy of Sciences Life Science School Yantai University

Objective Isoliquiritigenin （ISL）, a licorice chalconoid, is considered to be a bioactive agent with chemopreventive potential. This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells. Methods Cell viability was evaluated using a 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl-tetrazolium bromide （MTT） assay. Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit. The intracetlular ROS levels were assessed using a 2, 7-dichlorofluorescein probe assay. The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5, 5＇, 6, 6＇-tetra-chloro-1, 1＇, 3, 3＇-tetraethyl-imidacarbocyanine iodide （JC-1）. The degradation of poly-ADP-ribose polymerase （PARP） protein, the phosphorylation of PKR-like ER kinase （PERK）, the phosphorylation of the a-subunit of eukaryotic initiation factor 2 （elF2a）, the expression of the 78 kD glucose-regulated protein （GRP 78）, and the activation of caspase-12 were analyzed via western blot analysis. Results ISL significantly inhibited the proliferation, the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner. Moreover, ISL induced mitochondrial dysfunction, caspase activation, and PARP cleavage, which displayed features of mitochondria dependent on apoptotic signals. Besides, exposure of HeLa cells to ISL triggered endoplasmic reticulum （ER） stress, as indicated by the increase in p-elF2a and GRP78 expression, ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12. Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cel apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways.

关键词： ISL HeLa cells ROS Mitochondria ER stress Apoptosis

菠萝皮与不同比例王草和百香果藤混合青贮研究

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动物营养学报 2024年第1期36卷 637-649页

作者：崔智海李雪枫展小霞党佳皓王坚海南大学动物科技学院海口570228 海南大学林学院海口570228

本试验旨在探究菠萝皮、王草和百香果藤不同混合比例对青贮饲料发酵品质和微生物多样性的影响。以菠萝皮、王草和百香果藤为原料,将三者分别按鲜重比3∶6∶1、3∶5∶2、3∶4∶3、3∶3∶4和3∶2∶5分为5个组进行混合青贮,在室温条件下青... 详细信息

本试验旨在探究菠萝皮、王草和百香果藤不同混合比例对青贮饲料发酵品质和微生物多样性的影响。以菠萝皮、王草和百香果藤为原料,将三者分别按鲜重比3∶6∶1、3∶5∶2、3∶4∶3、3∶3∶4和3∶2∶5分为5个组进行混合青贮,在室温条件下青贮40 d后,对青贮饲料的发酵品质、营养成分含量和微生物多样性进行分析。结果表明:1)随着百香果藤混合比例的提高,青贮饲料pH以及氨态氮(NH 3-N)和丁酸(ba)含量呈提高趋势,其中3∶6∶1组pH最低,显著低于除3∶5∶2组外的其他组(Pba含量显著低于其他各组(Pbacillus),而3∶4∶3组和3∶3∶4组的优势菌门和优势菌属分别为变形菌门(Proteobacteria)和泛菌属(Pantoea)。综上所述,3∶6∶1组青贮饲料发酵效果最好,在青贮生产实践中推荐菠萝皮、王草和百香果藤的混合比例为3∶6∶1。

关键词：菠萝皮王草百香果藤混合青贮发酵品质微生物多样性

SR8278改善时差所致小鼠泪腺功能紊乱的转录组学分析

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眼科新进展 2024年第4期44卷 264-269页

作者：黄深振齐迪裴晓婷路顶立司宏丽黄杜鎏睿张文潇巴梦茹轩书婷李志杰郑州大学人民医院河南省人民医院河南大学人民医院河南省立眼科医院河南省眼科研究所河南省郑州市450003

目的探讨核受体亚家族1 D组成员1(NR1D1)合成拮抗剂SR8278在改善时差诱导的小鼠眶外泪腺结构和功能损伤方面的作用及其机制。方法 36只健康的8~10周龄野生型C57BL/6J小鼠在12 h光照/12 h黑暗循环的昼夜节律箱内适应2周后随机分为3组:... 详细信息

目的探讨核受体亚家族1 D组成员1(NR1D1)合成拮抗剂SR8278在改善时差诱导的小鼠眶外泪腺结构和功能损伤方面的作用及其机制。方法 36只健康的8~10周龄野生型C57BL/6J小鼠在12 h光照/12 h黑暗循环的昼夜节律箱内适应2周后随机分为3组:正常组(12 h光照/12 h黑暗循环的昼夜节律箱饲养)、时差组(8 h前移的12 h光照/12 h黑暗循环的昼夜节律箱饲养)及时差+SR8278组(8 h前移的12 h光照/12 h黑暗循环的昼夜节律箱饲养,并给予25 mg·kg^(-1)的SR8278干预),每组12只,共干预5 d。干预结束后,收集各组小鼠的活动度和核心体温,记录小鼠的泪液分泌量,并对泪腺组织进行称重和泪腺细胞大小测量。采用免疫组织化学方法对小鼠眶外泪腺进行组织学评估。提取泪腺RNA进行包含NR1D1的高通量RNA-Seq分析,并对获得的转录组数据进行KEGG和GO功能富集分析。结果与正常组相比,时差组小鼠白天活动度较高,夜间活动度较低,白天核心体温较高,夜间核心体温较低,差异均有统计学意义(均为P<0.05)。与时差组相比,时差+SR8278组小鼠白天活动度较低,夜间活动度较高,白天核心体温较低,夜间核心体温较高,差异均有统计学意义(均为P<0.05)。与正常组相比,时差组小鼠泪腺重量及泪液分泌量降低,泪腺细胞增大,差异均有统计学意义(均为P<0.05);与时差组相比,时差+SR8278组小鼠泪腺重量及泪液分泌量增加,泪腺细胞减小,差异均有统计学意义(均为P<0.05)。与正常组相比,时差组小鼠夜晚泪腺中NR1D1表达量较高;与时差组相比,时差+SR8278组小鼠夜晚泪腺中NR1D1表达量较低,差异均有统计学意义(均为P<0.05)。生物信息学分析显示,时差组和时差+SR8278组共有947个显著性差异基因,其中显著性上调基因43个,显著性下调基因904个,信号通路差异最大的为Notch信号通路。结论 SR8278通过靶向抑制NR1D1,有效增强了时差小鼠的泪液分泌功能,其过程可能是通过Notch信号通路完成的。

关键词：时差 SR8278 泪腺功能紊乱小鼠

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孕妇口腔保健健康教育的范围综述

中华现代护理杂志 2024年第19期30卷 2625-2636页

作者：谢业敏帅婷甘露党芸文晶诚宣岩李秀娥北京大学口腔医学院·口腔医院护理部北京100081 北京大学口腔医学院·口腔医院第二门诊部北京100020

目的:总结孕妇口腔保健健康教育的相关研究。方法:遵循范围综述的研究方法对文献进行描述分析,计算机检索PubMed、Embase、Web of Science、中国知网、万方数据库等7个数据库,检索时限为建库至2023年10月30日。结果:共纳入43篇文献。健... 详细信息

目的:总结孕妇口腔保健健康教育的相关研究。方法:遵循范围综述的研究方法对文献进行描述分析,计算机检索PubMed、Embase、Web of Science、中国知网、万方数据库等7个数据库,检索时限为建库至2023年10月30日。结果:共纳入43篇文献。健康教育的实施者主要为口腔专业人员和产前保健人员;理论基础包括健康信念模型、计划行为理论、社会认知理论等;方式包括传统授课或讲座、以家庭为中心、以互联网为载体、动机性访谈;内容涉及孕妇口腔保健多个方面;效果评价指标主要包括孕产妇口腔健康知-信-行水平及自我效能、口腔健康信念、口腔健康状态、口腔疾病患病率、不良妊娠结局及子女患龋率。结论:应建立口腔科与妇产科合作团队,将孕妇口腔保健纳入产前保健项目,充分利用孕妇学校平台,探索孕妇口腔保健健康教育的最优理论基础,完善孕妇口腔保健健康教育的内容;同时,通过开展高质量孕妇口腔健康教育的随访研究,促进母婴的口腔及整体健康。

关键词：综述孕妇口腔保健健康教育牙科

基于稀土上转换纳米粒子传感器的构建及其在食品添加剂检测中的应用

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光谱学与光谱分析 2024年第9期44卷 2459-2466页

作者：张璇王雅森温娜吕海霞李宝铭福州大学材料科学与工程学院分子材料与工程系福建福州350108

稀土上转换纳米粒子(UCNPs)因其具有毒性小、化学稳定性好和背景荧光低等优点被用于构建荧光纳米传感器。通过溶剂热法制备核壳UCNPs,采用柠檬酸钠对其进行表面配体交换得到水溶性核壳UCNPs(Cit-CS-UCNPs),以Cit-CS-UCNPs作为荧光传感... 详细信息

稀土上转换纳米粒子(UCNPs)因其具有毒性小、化学稳定性好和背景荧光低等优点被用于构建荧光纳米传感器。通过溶剂热法制备核壳UCNPs,采用柠檬酸钠对其进行表面配体交换得到水溶性核壳UCNPs(Cit-CS-UCNPs),以Cit-CS-UCNPs作为荧光传感器的能量供体,二氧化锰(MnO_(2))纳米片作为荧光传感器的能量受体,采用荧光共振能量转移(FRET)机理,构建了一种用于食品添加剂二氧化氢(H_(2)O_(2))和叔丁基对苯二酚(TBHQ)检测的荧光纳米传感器(Cit-CS-UCNPs-MnO_(2))。通过扫描电镜(SEM)、荧光光谱和紫外可见吸收光谱(UV-Vis)等分析测试手段对制备的纳米材料的形貌结构和性能进行表征。考察了猝灭剂浓度、孵育温度及孵育时间等实验条件对荧光传感体系检测性能的影响。荧光光谱和UV-Vis实验结果表明,Cit-CS-UCNPs最大发射峰位于654nm,Cit-CS-UCNPs与MnO_(2)结合后,核壳UCNPs发生荧光猝灭,当存在H_(2)O_(2)时,Cit-CS-UCNPs的荧光恢复,说明在此波段H_(2)O_(2)与MnO_(2)纳米片发生氧化还原反应,MnO_(2)纳米片被还原成Mn^(2+),逐渐从Cit-CS-UCNPs的表面解离下来;存在TBHQ时,Cit-CS-UCNPs-MnO_(2)与TBHQ体系峰位与TBHQ偏移到253nm,为TBHQ与MnO_(2)纳米片发生氧化还原反应,使得Cit-CS-UCNPs-MnO_(2)体系中FRET效应减弱,荧光强度回升。SEM结果表明,MnO_(2)纳米片均匀包覆在Cit-CS-UCNPs的周围,在水中仍可保持良好的分散性,表明MnO_(2)纳米片修饰到Cit-CS-UCNPs表面。对猝灭剂高锰酸钾(KMnO4)的浓度进行了优化。结果表明,当KMnO4浓度为10mol·L^(-1)时,猝灭效率可达90%。对检测条件进行优化,结果表明H_(2)O_(2)的检测孵育时间为25min时,MnO_(2)和H_(2)O_(2)之间氧化还原反应基本完全,Cit-CS-UCNPs-MnO_(2)荧光恢复值最大;TBHQ的检测孵育时间为30min。在最佳实验条件下,Cit-CS-UCNPs-MnO_(2)的荧光强度变化比率与H_(2)O_(2)浓度(0~1000μmol·L^(-1))及TBHQ浓度(0~0.6 mmol·L^(-1))分别具有良好的线性关系。保持最佳实验条件,选取食品中存在的代表性金属离子(如K+、Na+、Ca^(2+)和Mg^(2+))和常见的食品添加剂苯甲酸(ba)、D-葡萄糖(Glu)、山梨酸钾(PS)、蔗糖(Suc)、纳他霉素(Nat)和肌醇(Ino)作为研究对象进行选择性测试,发现与H_(2)O_(2)相比,Cit-CS-UCNPs-MnO_(2)对其他添加物质没有强烈的反应,传感器整体荧光信号波动不大,因此Cit-CS-UCNPs-MnO_(2)对H_(2)O_(2)和TBHQ可进行特异性检测。

关键词：上转换纳米粒子核壳结构荧光纳米传感器