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Chinese Chemical Letters 2004年第6期15卷 667-670页

作者： QinWEI danwu BinDU QingYuOU SchoolofChemistry＆ChemicalEngineering JinanUniversityJinan250022 LanzhouInstituteofChemicalPhysics ChineseAcademyofScienceLanzhou736000

The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(Ⅵ) complex combines protein rapidly to form a stable compound and the absorbance at 527 nm is in proportion to the concentration of protein in the range 0-16 μg mL-1 for bovine serum albumin (BSA). OP microemuslion media is introduced into protein determination, it has increased markedly the sensitivity of the system. The molar absorption coefficient was 5.98×l06 L mol-1 cm-1 for BSA. The assay, with sensitivity, simplicity and tolerance to many foreign substances, is applied to the determination of protein in samples with satisfactory results. Moreover, the binding number of BSA with the complex, which is determined by molar ratio and slope ratio methods, is in good agreement.

关键词： Protein spectrophotometry PF-Mo(Ⅵ) complex microemulsion enhanced sensitivity.

Microdetermination of Residual Protein in Penicillin by Resonance Light Scattering Technique with m-Nitrophenylfluorone-Mo（Ⅳ） Complex

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Chinese Chemical Letters 2005年第6期16卷 815-818页

作者： QinWEI danwu YahLI JunHongWANG YanLIU BinBU SchoolofChemistry＆ChemicalEngineering JinanUniversityJinan250022 SchoolofChemistry＆ChemicalEngineering JinanUniversityJinan250022／／KeyLaboratoryofColloidandInterfaceChemistryShandongUniversityJinan250100

Based on the enhancement of resonance light scattering (RLS) of m-nitrophenylfluorone -Mo(VI) complex by protein, a novel method for protein microdetermination in Tween 20 microemulsion has been developed. Under optimum condition, the linear ranges of bovine serum albumin are 0 - 0.03 μg-mL -1 with detection limits of 3.10 ng-mL-1. Most of amino acids and metal ions do not interfere. The method can be applied to determination of residual protein in penicillin Na salt and penicillin G potassium salt samples with satisfactory results.

关键词： Protein microemulsion m-NPF-Mo(VI) complex penicillin resonance light scattering.

Multi-Stage Hierarchical Channel Allocation in UAV-Assisted D2D Networks:A Stackelberg Game Approach

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China Communications 2021年第2期18卷 13-26页

作者： Tao Fang danwu MengWang Jiaxin Chen College of Communications Engineering Army Engineering University of PLANanjing 210007China College of Electronic and Information Engineering Nanjing University of Aeronautics and AstronauticsNanjing 211106China

UAV-assisted D2D networks can provide auxiliary communication for areas with poor communication facilities by using the characteristics of easy deployment of unmanned aerial vehicle(UAV),then it becomes a promising ***,the coexistence of UAV and D2D aggravates the conflict of spectrum *** addition,when the UAV performs the communication service,it will inevitably cause the location change,which will make the original channel allocation no longer *** by the influence of frequent channel switching on channel allocation,we define the communication utility as a tradeoff between the throughput and channel switching *** the considered model,we investigate the multi-stage hierarchical spectrum access problem with maximizing aggregate communication utilities in UAV-assisted D2D *** particular,due to the hierarchical feature of the considered network,we adopt Stackelberg game to formulate this spectrum access problem where both the throughput and channel switching cost are *** prove that the proposed game has a stable Stackelberg equilibrium(SE),and the heterogeneous network based channel allocation(HN-CA)algorithm is proposed to achieve the desired *** results verify the validity of the proposed game and show the effectiveness of the HN-CA algorithm.

关键词： UAV D2D multi-stage heterogeneous spectrum access stackelberg game

Stable expression of human cytochrome P450 2D6*10 in HepG2 cells

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World Journal of Gastroenterology 2004年第2期10卷 234-237页

作者： JianZhuge Ying-NianYu Xiao-danwu DepartmentofPathologyandPathophysiology SchoolofMedicineZhejiangUniversityHangzhou310031ZhejiangProvinceChina CenterofAnalysis ZhejiangUniversityHangzhou310031ZhejiangProvinceChina

AIM:Over 90% of drugs are metabolized by the cytochrome P-450 (CYP) family of liver *** most important enzymes are CYPIA2, 3A4,2C9/19,2D6 and *** CYP2D6 accounts for<2% of the total CYP liver enzyme content,it mediates metabolism in almost 25% of *** order to study its enzymatic activity for drug metabolism, its cDNA was cloned and a HepG2 cell line stably expressing CYP2D6 was ***:Human CYP2D6 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR) from total RNA extracted from human liver tissue and cloned into pGEM-T vector,cDNA segment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9.A cell line was established by transfecting the recombinant plasmid of pREP9-CYP2D6 to hepatoma HepG2 *** of mRNA was validated by *** activity of catalyzing dextromethorphan Odemethylation in postmitochondrial supernant (S9) fraction of the cells was determined by high performance liquid chromatography (HPLC).RESULTS:The cloned cDNA had 4 base differences, e.g.100 C→T, 336 T→C,408 C→G and 1 457 G→C,which resulted in P34S,and S486T amino acid substitutions, and two samesense mutations were 112F and 136V compared with that reported by Kimura et al (GenBank accession number: M33388).P34S and S486T amino acid substitutions were the characteristics of CYP2D6*10 *** relative activity of S9 fraction of HepG2-CYP2D6*10 metabolized detromethorphan O-demethylation was found to be 2.31±0.19nmol·min^-1·mg^-1 S9 protein (n=3),but was undetectable in parental HepG2 ***:cDNA of human CYP2D6*IOcan be successfully doned.A cell line,HepG2-CYP2D6*10,expressing CYP2D6*10 mRNA and having metabolic activity,has been established.

关键词： P-450细胞色素 HepG2细胞逆转录聚合酶链反应高效液相色谱法肝脏同功酶 DNA序列

Metastasis of primary gallbladder carcinoma in lymph node and liver

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World Journal of Gastroenterology 2005年第5期11卷 748-751页

作者： Han-TingLin Gui-JieLiu danwu Jian-YingLou DepartmentofGeneralSurgery theSecondAffiliatedHospitalofMedicalCollegeZhejiangUniversityHangzhou310009ZhejiangProvinceChina

AIM: To evaluate the patterns with metastasis of gallbladder carcinoma in lymph nodes and liver. METHODS: A total of 45 patients who had radical surgery were selected. The patterns with metastasis of primary gallbladder carcinoma in lymph nodes and liver were examined histopathologically and classified as TNM staging of the American Joint Committee on Cancer. RESULTS: Of the 45 patients, 29 (64.4%) had a lymph node positive disease and 20 (44.4%) had a direct invasion of the liver. The frequency of involvement of lymph nodes was strongly influenced by the depth of the primary tumor (P= 0.0001). The postoperative survival rate of patients with negative lymph node metastasis was significantly higher than that of patients with positive lymph node metastasis (P= 0.004), but the postoperative survival rate of patients with Nl lymph node metastasis was not significantly different from that of patients with N2 lymph node metastasis (P= 0.3874). The postoperative survival rate of patients without hepatic invasion was significantly better than that of patients with hepatic invasion (P= 0.0177). CONCLUSION: Complete resection of the regional lymph nodes is important in advanced primary gallbladder carcinoma (PGC). The initial sites of liver spread are located mostly in segments IV and V. It is necessary to achieve negative surgical margins 2 cm from the tumor. In patients with hepatic hilum invasion, extended right hepatectomy with or without bile duct resection or portal vein resection is necessary for curative resection.

关键词： Gallbladder carcinoma Liver cancer Lymph node metastasis

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1956—2010年中国降雪特征变化

清华大学学报（自然科学版） 2016年第4期56卷 381-386,393页

作者：章诞武丛振涛倪广恒清华大学水沙科学与水利水电工程国家重点实验室北京100084

降雪特征变化是气候变化的重要组成之一。降雪特征变化一方面影响流域径流总量,另一方面改变流域年内径流的分布。该文基于中国743个国家气象站1956—2010年日气象观测数据,在降雪辨识基础上,插值计算10km网格日降雪,然后从不同时间尺... 详细信息

降雪特征变化是气候变化的重要组成之一。降雪特征变化一方面影响流域径流总量,另一方面改变流域年内径流的分布。该文基于中国743个国家气象站1956—2010年日气象观测数据,在降雪辨识基础上,插值计算10km网格日降雪,然后从不同时间尺度分析了降雪特征变化。结果表明:除长江中下游外,中国主要降雪区域年降雪量都显著增加,并且存在"少—多—少"的代际变化模态;降雪在年内没有显著的集中趋势,有利于形成积雪,利于增加流域水资源量。长江中下游地区的降雪年内集中,年总降雪由主要日降雪事件构成,降雪对该地区的径流过程影响不大。

关键词：降雪趋势变化空间分布水文响应

1956—2010年中国水热季节性特征分析

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水科学进展 2015年第4期26卷 466-472页

作者：章诞武丛振涛倪广恒清华大学水利水电工程系水沙科学与水利水电工程国家重点实验室北京100084

流域水文平衡不仅决定于降水和潜在蒸散发总量,而且还受到其年内季节性变化特征的影响。收集1956—2010年中国743个国家级气象站逐日气候资料,采用FAO-Penman公式计算潜在蒸散发量,插值计算10 km网格日降水和潜在蒸发,进行水热季节性特... 详细信息

流域水文平衡不仅决定于降水和潜在蒸散发总量,而且还受到其年内季节性变化特征的影响。收集1956—2010年中国743个国家级气象站逐日气候资料,采用FAO-Penman公式计算潜在蒸散发量,插值计算10 km网格日降水和潜在蒸发,进行水热季节性特征的变化分析。结果表明,82.8%的区域潜在蒸散发量均值有下降趋势,但减少量不大,同时其年内季节性特征没有显著变化。表征降水年内波动振幅的季节性指数在全国范围均有显著下降趋势,且北方流域更为明显,变化率最大的西北诸河达到6.4%/10 a;峰值时间和雨季长度则变化不显著。这种水热季节性的变化被认为会导致流域径流的减少,在今后中国径流变化的归因研究中,应该考虑降水季节性变化的贡献。

关键词：水热季节性趋势检验气候变化水文响应

瓷都新语——“‘瓷的旅程’2023景德镇国际陶瓷艺术双年展”述评

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美术 2024年第5期 30-35,2,F0004页

作者：吕品昌谭丹武景德镇陶瓷大学九江学院

第二届景德镇国际陶瓷艺术双年展以景德镇为地理中心,以“瓷的旅程”为展览主题,展现出当下国际陶瓷艺术的时代面貌。“双年展”以开放和包容的姿态,向全世界展示了城市文化、陶瓷文化以及民族文化的多样性;陶艺创作者以全新的艺术视角... 详细信息

第二届景德镇国际陶瓷艺术双年展以景德镇为地理中心,以“瓷的旅程”为展览主题,展现出当下国际陶瓷艺术的时代面貌。“双年展”以开放和包容的姿态,向全世界展示了城市文化、陶瓷文化以及民族文化的多样性;陶艺创作者以全新的艺术视角和极具个人化的创新风格,表现出他们对陶瓷材料本体语言的持续探索。在这场艺术盛宴中,新技术媒介、新青年、新精神以创新且独特的方式大放异彩,展现了陶瓷艺术未来发展的无限可能。

关键词：景德镇陶瓷艺术中华文化美术交流

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中国传统建筑形式在当代雕塑中的再创作

雕塑 2021年第6期 66-67页

作者：谭丹武九江学院

近些年,对于传统艺术遗产的吸纳和借鉴在文化领域成为一个重要的现象,社会急剧变化产生的思想转变,也在不断影响着艺术家们对于传统艺术形式的态度和立场。我国传统建筑形式,因为它独特的形式和特殊的"中国式"身份,也成为当代艺术家借... 详细信息

近些年,对于传统艺术遗产的吸纳和借鉴在文化领域成为一个重要的现象,社会急剧变化产生的思想转变,也在不断影响着艺术家们对于传统艺术形式的态度和立场。我国传统建筑形式,因为它独特的形式和特殊的"中国式"身份,也成为当代艺术家借用的热点。特别是在与建筑同属于三维空间造型艺术的当代雕塑艺术中,对于借用传统建筑形式的再创作不乏亮点。这种双边视觉语言的交叉和冲突不断地撞击着雕塑家的创作思路,如何借助传统的内在价值,而跳脱出简单的形式外壳,当然也是艺术家们需要不断去挑战的新难题。

关键词：传统建筑形式当代雕塑艺术遗产文化价值

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论当代陶艺中的重复性语言表现形式

中国陶瓷工业 2022年第4期29卷 72-76页

作者：谭丹武九江学院

“重复”一直作为具有直接性和张力感的创作语言在当代艺术创作中被使用,陶瓷材料本身所特有的成型工艺和可复制性,为重复性语言表现形式在当代陶艺中的广泛运用提供了一定的前提条件。本文以重复性语言表现形式为切入点,从陶瓷材料的... 详细信息

“重复”一直作为具有直接性和张力感的创作语言在当代艺术创作中被使用,陶瓷材料本身所特有的成型工艺和可复制性,为重复性语言表现形式在当代陶艺中的广泛运用提供了一定的前提条件。本文以重复性语言表现形式为切入点,从陶瓷材料的特点出发,论述重复性创作形式在当代陶艺作品中的具体运用,通过对相关作品分析和归类,充分论述陶艺创作者在当代语境下的创作智慧。

关键词：重复性当代陶艺秩序感创作形式

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