Studies on the Phenylfluorone-Mo(Ⅵ) Complex as Interacting Mode Spectroscopic Probe of Protein in OP Microemulsion Medium

作     者：QinWEI DanWU BinDU QingYuOU 

作者机构：SchoolofChemistry＆ChemicalEngineeringJinanUniversityJinan250022 LanzhouInstituteofChemicalPhysicsChineseAcademyofScienceLanzhou736000 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2004年第15卷第6期

页      面：667-670页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 070301[理学-无机化学] 

基　　金：Financial support from Shangdong Provincial Science Foundation(Y2000B09) 

主　　题：Protein spectrophotometry PF-Mo(Ⅵ) complex microemulsion enhanced sensitivity. 

摘      要：The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(Ⅵ) complex combines protein rapidly to form a stable compound and the absorbance at 527 nm is in proportion to the concentration of protein in the range 0-16 μg mL-1 for bovine serum albumin (BSA). OP microemuslion media is introduced into protein determination, it has increased markedly the sensitivity of the system. The molar absorption coefficient was 5.98×l06 L mol-1 cm-1 for BSA. The assay, with sensitivity, simplicity and tolerance to many foreign substances, is applied to the determination of protein in samples with satisfactory results. Moreover, the binding number of BSA with the complex, which is determined by molar ratio and slope ratio methods, is in good agreement.