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肉鸡低蛋白日粮配制技术研究进展

饲料工业 2024年第9期45卷 1-9页

作者：袁建敏尹达菲中国农业大学动物科技学院畜禽饲养和营养国家重点实验室北京100193 沈阳农业大学动物科学与医学学院辽宁沈阳110866

低蛋白日粮关系到我国的畜禽生产、国际贸易,对于维持我国畜禽的可持续发展来说尤为重要。文章围绕肉鸡低蛋白日粮配制技术中涉及到的粗蛋白降低的程度、理想蛋白质氨基酸平衡模式、合成氨基酸补充种类、饲料原料标准回肠可消化氨基酸... 详细信息

低蛋白日粮关系到我国的畜禽生产、国际贸易,对于维持我国畜禽的可持续发展来说尤为重要。文章围绕肉鸡低蛋白日粮配制技术中涉及到的粗蛋白降低的程度、理想蛋白质氨基酸平衡模式、合成氨基酸补充种类、饲料原料标准回肠可消化氨基酸参数的研究进展进行综述,在限制性氨基酸得到满足的情况下,肉鸡日粮中粗蛋白最大可以降低2.5个百分点,添加蛋白酶或植酸酶粗蛋白可以降低0.5或1个百分点,低蛋白日粮配制还要注意电解质平衡。

关键词：肉鸡低蛋白日粮配制技术理想蛋白质氨基酸平衡模式合成氨基酸饲料

Insights into the proteomic profile of newly harvested corn and metagenomic analysis of the broiler intestinal microbiota

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Journal of Animal Science and Biotechnology 2022年第3期13卷 787-801页

作者： dafei Yin Youli Wang Liqun Wang Yuqin Wu Xiaoyi Bian Samuel E.Aggrey Jianmin yuan College of Animal Husbandry and Veterinary Medicine Shenyang Agricultural UniversityShenyang 110866China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural University100193 BeijingChina NutriGenomics Laboratory Department of Poultry ScienceUniversity of GeorgiaAthensGA 30602USA

Background:The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as“new season grain problem”.The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler ***:Newly harvested corn stored for either half a month(HM)or two months(TM)was used,and the pasting properties,total soluble sugars,and proteomic analysis technology was used to explore the influence of storage on natural aging corn ***,seventy-two 7-day-old Ross 308 male broiler chicken were fed with different stored *** metabolizable energy(AME),digesta viscosity,intestinal morphology and microbiota were examined to explore the influence of feed corn storage on broiler ***:Pasting properties in the TM corn exhibited decreased viscoelastic *** studies found a total of 26 proteins that were differentially expressed between the two treatment *** involved in starch and polysaccharides biosynthesis were upregulated in TM compared with *** fed on TM diet had higher relative energy utilization compared to the HM *** increased corn storage,the relative digesta viscosity decreased significantly(P≤0.05).The total number of goblet cells and lymphocytes was lower in chickens fed the TM *** microbiota data showed that the TM chickens had decreased abundance of diarrheal bacteria such as Hungatella hathewayi and Bacteroides fragilis,and increased butyrate-producing bacteria such as Alistipes compared to the HM ***:Storage of newly harvested corn induced the synthetic reaction of large molecules and changed the solubility of starch and protein with increasing soluble sugars and decreasing pasting properties that may improve the fermentation of intestinal microbiota,improve the energy utilization and protect gut health without the risk of diarrhe

关键词： Broilers Corn Metagenome Proteome Storage

Effect of storage time on the characteristics of corn and efficiency of its utilization in broiler chickens

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Animal Nutrition 2017年第3期3卷 252-257页

作者： dafei Yin Jianmin yuan Yuming Guo Lee I.Chiba State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Department of Animal Sciences College of Agriculture Auburn University

Corn is one of the staple food and feed ingredients in China, therefore its storage is of particular importance. Corn is typically stored for 2 or more years in national barns before it is sold as a food or feed ingredient. However, the effects of stored corn in national barns on the animal performance and nutrient utilization have not been investigated thus far. This study attempted to determine the effects of storage time on the chemical and physical characteristics of corn and its nutritional value, broiler growth performance, and meat quality. Corn grains used in the present study were stored for 4 different periods,from 2 to 5 yr, under the same conditions in a building at the Beijing National Grain Storage Facility. A total of 240 birds in Exp. 1 and 90 birds in Exp. 2 were used to compare the effects of storage time on the utilization of nutrients of corn, the performance, and meat quality of broilers. The content of starch, crude protein, amino acids, fatty acids, and test weight generally decreased with increasing storage time. Corn stored for over 4 yr showed decreased catalase(CAT) and peroxidase(POD) activities and increased fat acidity. Body weight gain(BWG) and European production index(EPI) of broilers from 0 to 3 wk tended to decrease linearly with storage time(0.05 0.05). The digestibility of histidine and arg

关键词： Broiler Corn Fat acidity Metabolizable energy Performance Storage

Antioxidant response and bioavailability of methionine hydroxy analog relative to DL-methionine in broiler chickens

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Animal Nutrition 2019年第3期5卷 241-247页

作者： Youli Wang Xiaonan Yin dafei Yin Zhao Lei Tahir Mahmood Jianmin yuan State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University

This study was designed to compare the effect of methionine(Met)sources(DL-methionine[DLM]and DL-2-hydroxy-4-methylthio-butanoic acid(HMTBa))and their supplementation levels on broiler growth performance and redox state.A 2 x 2 factorial arrangement was used with 2 sources(DLM and HMTBa)and 2 supplementation levels(0.05% and 0.252%)of Met.A total of 480 one-day-old broiler chicks were randomly divided into 4 treatments with 8 replicates per treatment(15 birds per replicate).The experiment lasted for 21 *** growth performance,redox capacity,redox-related genes expression,and Met transporters in different tissues were *** fed high Met supplementation levels had improved(P<0.05)body weight(BW).average daily gain(ADG)and feed conversion ratio(FCR).Similarly,broilers fed high Met levels had better(P<0.05)antioxidant abilities in the serum,small intestine,and ***,interactive effects(P<0.05)were also observed between Met sources and *** with *** fed HMTBa diets had decreased(P<0.05)total glutathione(T-GSH)and oxidized glutathione(GSSG)contents in duodenum,ileum,and ***,broilers fed HMTBa supplemented diets had increased(P<0.05)thioredoxin(Trx)gene expression in the duodenum and ileum,but decreased(P<0.05)glutaredoxin(Grx).glutathione reductase(CSR).and glutathione synthetase(GSS)genes ***,lower gene expression of Na+ and Cl-dependent neutral and cationic amino acid transporter(ATB0,+).and Na+ dependent neutral amino acid transporter(B0 AT)in the duodenum brush border,but higher gene expression of diamine acetyltransferase 1(SAT1)and Na+-independent branched-chain and aromatic amino acid transporter(LAT1)in the jejunum and ileum basement membrane along with higher expression of the proton dependent monocarboxylate transporter 1(MCT1)gene in the ileum were detected in birds fed HMTBa *** *** can be effectively used in glutathione synthesis to exert antioxidant functions,whereas HMTBa

关键词： Amino acid transporter Broiler DL-methionine Methionine hydroxy analog Oxidation resistance

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碳交易下考虑补贴的供应链定价与拆卸性决策

计算机集成制造系统 2023年第8期29卷 2830-2845页

作者：袁开福王盼盼王大飞任廷海贵州财经大学工商管理学院贵州贵阳550025

碳交易机制下,为确定产品最优价格和可拆卸性水平,针对考虑补贴且制造商和再制造商存在竞争的供应链,构建了制造商不进行/进行可拆卸性设计的博弈模型,得到了两类模型的均衡解,并进行了比较和算例分析。结果表明,制造商进行可拆卸性设... 详细信息

碳交易机制下,为确定产品最优价格和可拆卸性水平,针对考虑补贴且制造商和再制造商存在竞争的供应链,构建了制造商不进行/进行可拆卸性设计的博弈模型,得到了两类模型的均衡解,并进行了比较和算例分析。结果表明,制造商进行可拆卸性设计会提高供应链成员利润和降低产品价格;制造商不能单纯依靠降低可拆卸性水平来抑制再制造商发展,因为当可拆卸性水平敏感系数对再制造品生产成本的影响远大于对新品生产成本的影响时,制造商应提高产品的可拆卸性水平;政府增加再制造补贴并不总是有利于再制造产业发展,因为当可拆卸性水平敏感系数对再制造品和新品生产成本的影响较接近且可拆卸性研发成本系数较小时,增加补贴会降低再制造品需求;算例分析表明维持较高的碳交易价格有利于提高产品售价、可拆卸性水平和供应链成员利润。

关键词：再制造供应链碳交易政府补贴产品可拆卸性

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碳交易下考虑质量升级的再制造供应链定价

计算机集成制造系统 2022年第5期28卷 1586-1602页

作者：袁开福吴光强何波任廷海王大飞贵州财经大学工商管理学院贵州贵阳550025 重庆大学经济与工商管理学院重庆400030

为了确定考虑质量升级的再制造供应链定价决策。首先,根据历史强度法分配免费碳配额;其次,构建了不进行/进行产品质量升级的再制造供应链差别定价决策模型,采用逆向归纳法得到两种模型的最优定价及质量升级策略;最后,对两类模型进行比较... 详细信息

为了确定考虑质量升级的再制造供应链定价决策。首先,根据历史强度法分配免费碳配额;其次,构建了不进行/进行产品质量升级的再制造供应链差别定价决策模型,采用逆向归纳法得到两种模型的最优定价及质量升级策略;最后,对两类模型进行比较,并通过算例对相关结论进行验证。研究发现:①质量升级有利于提升产品的质量水平、销售价格、市场需求和供应链成员及系统利润;②提高减排目标和碳交易价格会引起产品质量升级程度、市场需求和供应链成员及系统利润下降,但对碳减排有利;③提高质量升级效率、消费者质量敏感度和废旧品质量水平有助于提升产品质量水平、销售价格、市场需求和供应链成员及系统利润。因此,制造商应该进行质量升级、提高质量升级效率和废旧品质量水平来获取更多利润;政府应该合理设定减排目标来平衡减排效果和供应链获利状况。

关键词：碳交易机制产品质量升级再制造供应链差别定价

Supplementation of amylase combined with glucoamylase or protease changes intestinal microbiota diversity and benefits for broilers fed a diet of newly harvested corn

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Journal of Animal Science and Biotechnology 2018年第2期9卷 467-479页

作者： dafei Yin Xiaonan Yin Xingyu Wang Zhao Lei Maofei Wang Yuming Guo Samuel E.Aggrey Wei Nie Jianmin yuan State key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina NutriGenomics Laboratory Department of Poultry ScienceUniversity of GeorgiaAthensGAUSA

Background: The effect of amylases combined with exogenous carbohydrase and protease in a newly harvested corn diet on starch digestibility, intestine health and cecal microbiota was investigated in broiler ***: Two hunderd and eighty-eight 5-day-old female chickens were randomly divided into six treatments: a newly harvested corn-soybean meal diet(control); control supplemented with 1,500 U/g α-amylase(Enzyme A);Enzyme A + 300 U/g amylopectase + 20,000 U/g glucoamylase(Enzyme B); Enzyme B + protease 10,000 U/g(Enzyme C); Enzyme C + xylanase 15,000 U/g(Enzyme D); and Enzyme D + cellulase 200 U/g + pectinase 1,000 U/g(Enzyme E). Growth performance, starch digestibility, digestive organ morphology, and intestinal microbiota were evaluated in the birds at 16 and 23 d of ***: Compared with the control diet, supplementation with Enzyme A significantly decreased ileum lesion scoring at 16 d of age(P < 0.05); supplementation with Enzyme B or Enzyme C showed positive effects on ileal amylopectin and total starch digestibility(P < 0.05); Broilers fed with a diet supplemented with Enzyme D had a tendency to decrease body weight gain at 23 d. Enzyme E supplementation improved lesion scoring of jejunum and ileum at 16 d(P < 0.05), and increased ileal amylopectin or total starch digestibility at 23 d(P < 0.05).Supplementation of enzymes changed cecal microbiota diversity. High numbers of Campylobacter, Helicobacter and Butyricicoccus, Anaerostipes and Bifidobacterium, Sutterella and Odoribacter were the main genera detected in supplementations with Enzymes B, C, D, and E ***: Supplementation with amylase combined with glucoamylase or protease showed a beneficial effect on starch digestibility and intestinal microbiota diversity, and increased growth of broilers fed with newly harvested corn.

关键词： Amylase Broiler Glucoamylase Microbiota diversity Newly harvested corn Protease

Influence of starch sources and dietary protein levels on intestinal functionality and intestinal mucosal amino acids catabolism in broiler chickens

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Journal of Animal Science and Biotechnology 2019年第3期10卷 658-672页

作者： dafei Yin Peter H.Selle Amy F.Moss Youli Wang Xiaoyu Dong Zhibin Xiao Yuming Guo Jianmin yuan State key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China Poultry Research Foundation The University of SydneyWerombi RoadCamdenNSW 2570Australia

Background: There is growing interest in carbohydrate and protein nutrition to enhance the efficiency of animal ***-crude protein diets depress environmental pollution and feeding cost,but the challenge to their adoption is maintaining digestive function and growth performance of *** present study was conducted to evaluate the influence of different dietary starch sources and protein levels on intestinal functionality and mucosal amino acid ***: Six dietary treatments,based on maize and soybean meal,were offered to 360 AA+male chicks from 6 to 35 d post-hatch as a 3 × 2 factorial *** waxy rice or amylose was added to a conventional maize-soy diet to provide three sources of starch with different digestion rates and relatively high and low dietary protein *** performance,parameters of intestinal functionality and concentrations of free amino acid in the portal circulation were ***: In the grower phase,starch source influenced(P < 0.02) weight gain as diets containing amylose supported significantly higher weight gains than waxy *** increase of ileal ATP concentrations and Na^+/K^+-ATPase activity were found in amylose ***,amylose decreased BrdU positive cell numbers and down-regulated m RNA expression for *** activity in the ileum was higher(P < 0.01) in birds offered low protein diets and there was a trend(P = 0.057) for waxy rice as a starch source to increase ileal GOT *** was a significant influence on the concentration of seventeen amino acids in the portal circulation with tryptophan the one *** rice as a starch source generated 13.6% and 22.4% numerically higher concentrations of non-essential amino acids than maize and amylose,***: Amino acid catabolism in the gut mucosa is subject to nutritional *** that amino acids can be spared from catabolism in the gut mucosa by supplementation of amylose,it follow

关键词： Amino acids Broiler chickens Enterocytes Glucose Starch

阿拉伯木聚糖和阿拉伯低聚木糖的益生功能研究进展

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动物营养学报 2017年第2期29卷 365-373页

作者：雷钊尹达菲袁建敏中国农业大学动物科技学院北京100193

畜禽肠道健康的调控已成为当今畜牧业中的重点关注问题。阿拉伯木聚糖(AX)和阿拉伯低聚木糖(AXOS)作为新型的益生元,其对双歧杆菌等益生菌的增殖效果优于低聚果糖等益生元。此外,与低聚果糖等益生元相比,AX和AXOS能够进入肠道后段进行发... 详细信息

畜禽肠道健康的调控已成为当今畜牧业中的重点关注问题。阿拉伯木聚糖(AX)和阿拉伯低聚木糖(AXOS)作为新型的益生元,其对双歧杆菌等益生菌的增殖效果优于低聚果糖等益生元。此外,与低聚果糖等益生元相比,AX和AXOS能够进入肠道后段进行发酵,生成非支链的短链脂肪酸,并抑制蛋白质的发酵,减少有毒物质(苯酚、氨等)的生成,从而有效地调节后肠段(结肠和盲肠)的健康。不同结构的AX和AXOS具有不同的益生功能,且AX和AXOS间存在协同增效的益生作用。本文综述了近年来关于AX和AXOS的益生功能的研究进展,为其在畜牧业中的应用提供一定的理论依据和指导。

关键词：阿拉伯木聚糖阿拉伯低聚木糖益生功能肠道健康