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Antioxidant response and bioavailability of methionine hydroxy analog relative to DL-methionine in broiler chickens

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Animal Nutrition 2019年第3期5卷 241-247页

作者： Youli Wang Xiaonan yin dafei yin Zhao Lei Tahir Mahmood Jianmin Yuan State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University

This study was designed to compare the effect of methionine(Met)sources(DL-methionine[DLM]and DL-2-hydroxy-4-methylthio-butanoic acid(HMTBa))and their supplementation levels on broiler growth performance and redox state.A 2 x 2 factorial arrangement was used with 2 sources(DLM and HMTBa)and 2 supplementation levels(0.05% and 0.252%)of Met.A total of 480 one-day-old broiler chicks were randomly divided into 4 treatments with 8 replicates per treatment(15 birds per replicate).The experiment lasted for 21 *** growth performance,redox capacity,redox-related genes expression,and Met transporters in different tissues were *** fed high Met supplementation levels had improved(P<0.05)body weight(BW).average daily gain(ADG)and feed conversion ratio(FCR).Similarly,broilers fed high Met levels had better(P<0.05)antioxidant abilities in the serum,small intestine,and ***,interactive effects(P<0.05)were also observed between Met sources and *** with *** fed HMTBa diets had decreased(P<0.05)total glutathione(T-GSH)and oxidized glutathione(GSSG)contents in duodenum,ileum,and ***,broilers fed HMTBa supplemented diets had increased(P<0.05)thioredoxin(Trx)gene expression in the duodenum and ileum,but decreased(P<0.05)glutaredoxin(Grx).glutathione reductase(CSR).and glutathione synthetase(GSS)genes ***,lower gene expression of Na+ and Cl-dependent neutral and cationic amino acid transporter(ATB0,+).and Na+ dependent neutral amino acid transporter(B0 AT)in the duodenum brush border,but higher gene expression of diamine acetyltransferase 1(SAT1)and Na+-independent branched-chain and aromatic amino acid transporter(LAT1)in the jejunum and ileum basement membrane along with higher expression of the proton dependent monocarboxylate transporter 1(MCT1)gene in the ileum were detected in birds fed HMTBa *** *** can be effectively used in glutathione synthesis to exert antioxidant functions,whereas HMTBa

关键词： Amino acid transporter Broiler DL-methionine Methionine hydroxy analog Oxidation resistance

Effect of storage time on the characteristics of corn and efficiency of its utilization in broiler chickens

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Animal Nutrition 2017年第3期3卷 252-257页

作者： dafei yin Jianmin Yuan Yuming Guo Lee I.Chiba State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Department of Animal Sciences College of Agriculture Auburn University

Corn is one of the staple food and feed ingredients in China, therefore its storage is of particular importance. Corn is typically stored for 2 or more years in national barns before it is sold as a food or feed ingredient. However, the effects of stored corn in national barns on the animal performance and nutrient utilization have not been investigated thus far. This study attempted to determine the effects of storage time on the chemical and physical characteristics of corn and its nutritional value, broiler growth performance, and meat quality. Corn grains used in the present study were stored for 4 different periods,from 2 to 5 yr, under the same conditions in a building at the Beijing National Grain Storage Facility. A total of 240 birds in Exp. 1 and 90 birds in Exp. 2 were used to compare the effects of storage time on the utilization of nutrients of corn, the performance, and meat quality of broilers. The content of starch, crude protein, amino acids, fatty acids, and test weight generally decreased with increasing storage time. Corn stored for over 4 yr showed decreased catalase(CAT) and peroxidase(POD) activities and increased fat acidity. Body weight gain(BWG) and European production index(EPI) of broilers from 0 to 3 wk tended to decrease linearly with storage time(0.05 0.05). The digestibility of histidine and arg

关键词： Broiler Corn Fat acidity Metabolizable energy Performance Storage

Supplementation of amylase combined with glucoamylase or protease changes intestinal microbiota diversity and benefits for broilers fed a diet of newly harvested corn

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Journal of Animal Science and Biotechnology 2018年第2期9卷 467-479页

作者： dafei yin Xiaonan yin Xingyu Wang Zhao Lei Maofei Wang Yuming Guo Samuel E.Aggrey Wei Nie Jianmin Yuan State key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina NutriGenomics Laboratory Department of Poultry ScienceUniversity of GeorgiaAthensGAUSA

Background: The effect of amylases combined with exogenous carbohydrase and protease in a newly harvested corn diet on starch digestibility, intestine health and cecal microbiota was investigated in broiler ***: Two hunderd and eighty-eight 5-day-old female chickens were randomly divided into six treatments: a newly harvested corn-soybean meal diet(control); control supplemented with 1,500 U/g α-amylase(Enzyme A);Enzyme A + 300 U/g amylopectase + 20,000 U/g glucoamylase(Enzyme B); Enzyme B + protease 10,000 U/g(Enzyme C); Enzyme C + xylanase 15,000 U/g(Enzyme D); and Enzyme D + cellulase 200 U/g + pectinase 1,000 U/g(Enzyme E). Growth performance, starch digestibility, digestive organ morphology, and intestinal microbiota were evaluated in the birds at 16 and 23 d of ***: Compared with the control diet, supplementation with Enzyme A significantly decreased ileum lesion scoring at 16 d of age(P < 0.05); supplementation with Enzyme B or Enzyme C showed positive effects on ileal amylopectin and total starch digestibility(P < 0.05); Broilers fed with a diet supplemented with Enzyme D had a tendency to decrease body weight gain at 23 d. Enzyme E supplementation improved lesion scoring of jejunum and ileum at 16 d(P < 0.05), and increased ileal amylopectin or total starch digestibility at 23 d(P < 0.05).Supplementation of enzymes changed cecal microbiota diversity. High numbers of Campylobacter, Helicobacter and Butyricicoccus, Anaerostipes and Bifidobacterium, Sutterella and Odoribacter were the main genera detected in supplementations with Enzymes B, C, D, and E ***: Supplementation with amylase combined with glucoamylase or protease showed a beneficial effect on starch digestibility and intestinal microbiota diversity, and increased growth of broilers fed with newly harvested corn.

关键词： Amylase Broiler Glucoamylase Microbiota diversity Newly harvested corn Protease

Insights into the proteomic profile of newly harvested corn and metagenomic analysis of the broiler intestinal microbiota

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Journal of Animal Science and Biotechnology 2022年第3期13卷 787-801页

作者： dafei yin Youli Wang Liqun Wang Yuqin Wu Xiaoyi Bian Samuel E.Aggrey Jianmin Yuan College of Animal Husbandry and Veterinary Medicine Shenyang Agricultural UniversityShenyang 110866China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural University100193 BeijingChina NutriGenomics Laboratory Department of Poultry ScienceUniversity of GeorgiaAthensGA 30602USA

Background:The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as“new season grain problem”.The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler ***:Newly harvested corn stored for either half a month(HM)or two months(TM)was used,and the pasting properties,total soluble sugars,and proteomic analysis technology was used to explore the influence of storage on natural aging corn ***,seventy-two 7-day-old Ross 308 male broiler chicken were fed with different stored *** metabolizable energy(AME),digesta viscosity,intestinal morphology and microbiota were examined to explore the influence of feed corn storage on broiler ***:Pasting properties in the TM corn exhibited decreased viscoelastic *** studies found a total of 26 proteins that were differentially expressed between the two treatment *** involved in starch and polysaccharides biosynthesis were upregulated in TM compared with *** fed on TM diet had higher relative energy utilization compared to the HM *** increased corn storage,the relative digesta viscosity decreased significantly(P≤0.05).The total number of goblet cells and lymphocytes was lower in chickens fed the TM *** microbiota data showed that the TM chickens had decreased abundance of diarrheal bacteria such as Hungatella hathewayi and Bacteroides fragilis,and increased butyrate-producing bacteria such as Alistipes compared to the HM ***:Storage of newly harvested corn induced the synthetic reaction of large molecules and changed the solubility of starch and protein with increasing soluble sugars and decreasing pasting properties that may improve the fermentation of intestinal microbiota,improve the energy utilization and protect gut health without the risk of diarrhe

关键词： Broilers Corn Metagenome Proteome Storage

Architecture and key technologies of grid geographic information system

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Science China(Technological Sciences) 2008年第S1期51卷 102-113页

作者： FANG Yu,HUANG Zhou,CHEN Bin,WU Lun & yin dafei Institute of Remote Sensing and Geographic Information System,Peking University,Beijing 100871,China Institute of Remote Sensing and Geographic Information System Peking University Beijing China

The highly efficient and stable collaborative computation platform for geospatial information can be constructed on the basis of Grid computing technology,com-bined with Peer-to-Peer (P2P) computing technology and geospatial database technology. This paper proposed the architecture and key technologies of the Grid GIS (Geographic Information System) incorporated with P2P structure,and corre-spondingly a Grid GIS prototype named Nebula was designed and then imple-mented. Nebula is a suite of middleware for geospatial Grid computing,which could be deployed onto various service nodes in network. Based on Grid protocols and infrastructure,Nebula provides invocation interfaces to users in form of Grid services. By using P2P message based communication mechanism,complex geospatial computation tasks could be accomplished by Nebula in a collaborative way. This paper introduced Nebula's architecture and key modules,and according to experimental data,we discussed the Grid GIS's advantages,application scenar-ios and future directions.

关键词： Grid GIS distributed geospatial computing Peer-to-Peer computing

动物科学专业《饲料学》教学模式的改革探索与思考

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现代畜牧科技 2024年第4期 163-165页

作者：张瑞阳李方方尹达菲朱宇旌张勇沈阳农业大学动物科学与医学学院辽宁沈阳110866

《饲料学》是动物科学的一门专业核心课,是研究饲料分类、营养价值及其用途前景的科学。随着学科发展以及教育理念的不断发展与更新,对《饲料学》教学模式进行改革十分必要。该文从教学设计、教学方法、课程考核等方面介绍《饲料学》教... 详细信息

《饲料学》是动物科学的一门专业核心课,是研究饲料分类、营养价值及其用途前景的科学。随着学科发展以及教育理念的不断发展与更新,对《饲料学》教学模式进行改革十分必要。该文从教学设计、教学方法、课程考核等方面介绍《饲料学》教学改革的具体内容与措施,以期为该课程的教学模式改革奠定理论基础。

关键词：饲料学课程思政教学方法课程考核

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儒家文化对新加坡师生关系的影响

外国中小学教育 2010年第11期 53-57页

作者：尹达斐首都师范大学教育学院

新加坡是华族人口占主体的国家,儒家文化的传播以及华语教学的推动,使得新加坡教育体现出浓烈的儒学色彩,其师生关系也呈现出鲜明的儒家特色。儒家文化对新加坡师生关系的建构与发展产生着重要影响。

关键词：新加坡儒家文化师生关系

DNA-delivered monoclonal antibodies targeting the p53 R175H mutant epitope inhibit tumor development in mice

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Genes & Diseases 2024年第4期11卷 368-380页

作者： dafei Chai Xu Wang Praveen Neeli Shan Zhou Xingfang Yu Kanaga Sabapathy Yong Li Department of Medicine Section of Epidemiology and Population SciencesDan L Duncan Comprehensive Cancer CenterBaylor College of MedicineHoustonTX 77030USA Division of Cellular&Molecular Research Humphrey Oei Institute of Cancer ResearchNational Cancer Centre SingaporeSingapore 168583Singapore

tThe tumor suppressor p53 is the most common mutated gene in cancer,with the R175H as the most frequent p53 missense ***,there are currently no approved targeted therapies or immunotherapies against mutant ***,we characterized and inves-tigated a monoclonal antibody(mAb)that recognizes the mutant p53-R175H for its affinity,specificity,and activity against tumor cells in *** then delivered DNA plasmids expres-sing the anti-R175H mAb or a bispecific antibody(BsAb)into mice to evaluate their therapeutic *** results showed that the anti-R175H mAb specifically bound to the p53-R175H an-tigen with a high affinity and recognized the human mutant p53-R175H antigen expressed on HEK293T or MC38 cells,with no cross-reactivity with wild-type *** cultured cells,the anti-R175H mAb showed higher cytotoxicity than the control but did not induce antibody-dependent cellular *** made a recombinant MC38 mouse cell line(MC38-p53-R175H)that overexpressed the human p53-R175H after knocking out the endogenous mutant p53 *** vivo,administration of the anti-R175H mAb plasmid elicited a robust anti-tumor effect against MC38-p53-R175H in *** administration of the anti-R175H BsAb plasmid showed no therapeutic effects,yet potent anti-tumor activity was observed in combination with the anti-PD-1 *** results indicate that targeting specific mutant epitopes using DNA-delivered mAbs or BsAbs presents a form of improved natural immunity derived from tumor-infiltrating B cells and plasma cells against intracellular tumor antigens.

关键词： BsAb Cytotoxicity mAb Mutant p53 PD-1 R175H

Influence of starch sources and dietary protein levels on intestinal functionality and intestinal mucosal amino acids catabolism in broiler chickens

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Journal of Animal Science and Biotechnology 2019年第3期10卷 658-672页

作者： dafei yin Peter H.Selle Amy F.Moss Youli Wang Xiaoyu Dong Zhibin Xiao Yuming Guo Jianmin Yuan State key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China Poultry Research Foundation The University of SydneyWerombi RoadCamdenNSW 2570Australia

Background: There is growing interest in carbohydrate and protein nutrition to enhance the efficiency of animal ***-crude protein diets depress environmental pollution and feeding cost,but the challenge to their adoption is maintaining digestive function and growth performance of *** present study was conducted to evaluate the influence of different dietary starch sources and protein levels on intestinal functionality and mucosal amino acid ***: Six dietary treatments,based on maize and soybean meal,were offered to 360 AA+male chicks from 6 to 35 d post-hatch as a 3 × 2 factorial *** waxy rice or amylose was added to a conventional maize-soy diet to provide three sources of starch with different digestion rates and relatively high and low dietary protein *** performance,parameters of intestinal functionality and concentrations of free amino acid in the portal circulation were ***: In the grower phase,starch source influenced(P < 0.02) weight gain as diets containing amylose supported significantly higher weight gains than waxy *** increase of ileal ATP concentrations and Na^+/K^+-ATPase activity were found in amylose ***,amylose decreased BrdU positive cell numbers and down-regulated m RNA expression for *** activity in the ileum was higher(P < 0.01) in birds offered low protein diets and there was a trend(P = 0.057) for waxy rice as a starch source to increase ileal GOT *** was a significant influence on the concentration of seventeen amino acids in the portal circulation with tryptophan the one *** rice as a starch source generated 13.6% and 22.4% numerically higher concentrations of non-essential amino acids than maize and amylose,***: Amino acid catabolism in the gut mucosa is subject to nutritional *** that amino acids can be spared from catabolism in the gut mucosa by supplementation of amylose,it follow

关键词： Amino acids Broiler chickens Enterocytes Glucose Starch