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Electrical stimulation of acupoint combinations against deep venous thrombosis in elderly bedridden patients after major surgery

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Journal of Traditional Chinese Medicine 2013年第2期33卷 187-193页

作者： Lili Hou cuiping Chen Lei Xu PeihaoYin Wen Peng Department of Nursing Shanghai Pulmonary Hospital Department of Nursing Tenth People's Hospital of Tongji University Department of Nursing Putuo HospitalShanghai University of Chinese Medicine Department of General Surgery Putuo HospitalShanghai University of Chinese Medicine Department of Kidney Putuo HospitalShanghai University of Chinese Medicine

OBJECTIVE:To compare the effects of electrical stimulation of different acupoint combinations among postoperative bedridden elderly patients on hemorheology and deep venous blood flow velocity and investigate the role of electrical stimulation against deep vein thrombosis(DVT).METHODS:From November 2010 to October 2011,a total of 160 elderly bedridden patients after major surgery were divided into the conventional care group,invigorating and promoting Qi group,blood-activating and damp-eliminating group,and acupoint-combination stimulation *** blood viscosity,plasma viscosity,D-dimer levels,lower limb skin temperature,lower limb circumference,and flow velocities of the external iliac vein,femoral vein,popliteal vein,and deep calf veins in all patients were documented and compared among the four ***:Whole blood viscosity,plasma viscosity,D-dimer levels,and lower limb circumference were significantly reduced in the blood-activating and damp-eliminating group compared with the conventional care group(P0.05).Lower limb venous flow velocities were accelerated in the invigorating and promoting Qi group compared with the other groups,excluding the acupoint-combination stimulation group(P<0.05).CONCLUSION:Hemorheological indices in postoperative bedridden elderly patients were improved after combined electrical stimulation at Yinlingquan(SP 9) and Sanyinjiao(SP 6).Combined electrical stimulation at Zusanli(ST 36) and Taichong(LR 3),on the other hand,accelerated lower limb venous flow.

关键词： Electric stimulation Acupuncturepoint Venous thrombosis Blood flow velocity Aged Nursing care

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乳酸菌菌株分离鉴定

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食品安全导刊 2024年第23期 87-90,98页

作者：程凯悦李翠平麻士卫孙洁宇殷文政闫继彪内蒙古普泽生物制品有限责任公司内蒙古呼和浩特010018

为从牧区筛选的天然样品奶酪和酸奶中分离出有研究价值的乳酸菌,本文采用涂布、划线分离目标菌株,并对其进行初步的形态学鉴定;通过分析生长过程中的pH值、OD值、活菌数的变化规律,判定其是否符合乳酸菌生长特征;经16S rDNA测序,确定菌... 详细信息

为从牧区筛选的天然样品奶酪和酸奶中分离出有研究价值的乳酸菌,本文采用涂布、划线分离目标菌株,并对其进行初步的形态学鉴定;通过分析生长过程中的pH值、OD值、活菌数的变化规律,判定其是否符合乳酸菌生长特征;经16S rDNA测序,确定菌株类别。经NCBI数据库对比,得到5株乳酸菌,分别为嗜热链球菌(Streptococcus thermophilus)、植物乳杆菌(Lactobacillus plantarum)、动物双歧杆菌(Bifidobacterium animalis)、保加利亚乳杆菌(Lactobacillus bulgaricus)及干酪乳杆菌(Lactobacillus casei)。

关键词：乳酸菌分离鉴定菌群

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铸造企业突发环境事件应急预案编制要点分析

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中国铸造装备与技术 2023年第4期58卷 113-116页

作者：马翠萍韩小问勾东东河北省沧州生态环境监测中心河北沧州061000 河北欣众环保科技有限公司河北沧州061000

铸造产业作为沧州市的支柱产业之一,具有企业数量多、规模大的特点,同时也是生态环境重点监管行业。面对新形势下生态环境管理要求,梳理突发环境事件应急预案的章节内容,分析铸造企业环境风险特点,总结出应急预案编制要点和注意事项。

关键词：铸造突发环境事件应急预案

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人工诱导东北林蛙苏醒适应机制分析

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安徽农学通报 2024年第11期30卷 49-55页

作者：赵红蕾张传辉孟如愿马佳欣文仁霜于翠平东北林业大学野生动物与自然保护地学院黑龙江哈尔滨150040

为了探究东北林蛙在人工诱导苏醒过程中适应温度变化的生理机制,包括不同温度、不同器官或组织的冷冻保护剂含量变化及其抗氧化应激相关生理指标在苏醒适应阶段的差异,采用梯度升温方法对冬眠东北林蛙进行人工诱导苏醒,至东北林蛙摄食... 详细信息

为了探究东北林蛙在人工诱导苏醒过程中适应温度变化的生理机制,包括不同温度、不同器官或组织的冷冻保护剂含量变化及其抗氧化应激相关生理指标在苏醒适应阶段的差异,采用梯度升温方法对冬眠东北林蛙进行人工诱导苏醒,至东北林蛙摄食标记苏醒过程结束。苏醒培养期间取样,未取样林蛙持续培养,取样结束后剩余林蛙常温养殖;采用分光光度法对其肝脏、肌肉内的葡萄糖含量、尿素氮含量、总抗氧化能力进行测定。结果表明,东北林蛙肝脏内上述物质含量与肌肉内各物质的消耗趋势基本一致,但肝脏内各物质含量及其变化幅度远大于肌肉内。冬眠东北林蛙人工诱导苏醒初期葡萄糖、尿素氮含量即冷冻保护剂含量明显降低(P<0.05)、总抗氧化能力明显增强(P<0.05),而苏醒后期,各项含量趋于稳定,无明显变化。这说明肝脏是东北林蛙冬眠过程中对抗低温冷冻环境的主要器官,且冬眠状态的东北林蛙体内会积累冷冻保护剂,随着苏醒过程的进行,温度逐渐上升,机体活动逐渐恢复,葡萄糖被大量消耗,以对抗因缺少能量来源而带来的应激损伤,抗氧化应激能力也随之增强以维持内环境稳定,保证东北林蛙在0℃以下得以生存。

关键词：东北林蛙人工诱导苏醒冷冻保护剂抗氧化能力人工养殖

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铸造企业地块场地环境初步调查要点分析

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中国铸造装备与技术 2023年第5期58卷 46-50页

作者：马翠萍韩小问勾东东河北省沧州生态环境监测中心河北沧州061000 河北欣众环保科技有限公司河北沧州061000

目前产业政策不允许新增铸造产能,只能产能置换,同时行业重组导致部分铸造企业退出市场。铸造企业结束原有生产经营活动,改变原土地使用性质时,须对原址土壤和地下水进行污染监测分析和评价。面对新的生态环境管理要求,梳理铸造企业地... 详细信息

目前产业政策不允许新增铸造产能,只能产能置换,同时行业重组导致部分铸造企业退出市场。铸造企业结束原有生产经营活动,改变原土地使用性质时,须对原址土壤和地下水进行污染监测分析和评价。面对新的生态环境管理要求,梳理铸造企业地块场地环境初步调查内容,分析铸造企业场地调查区域和污染物确定,总结出地块场地环境初步调查编制要点和注意事项。

关键词：铸造场地环境调查

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济宁市生活垃圾渗滤液的电化学氧化法处理技术探析

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环境科学与管理 2015年第2期40卷 70-73页

作者：马翠萍贾翠娟冯兴丽济宁职业技术学院山东济宁272037

目前,生活垃圾污染已经日趋严重,而生活垃圾渗滤液对环境造成的危害更是不容忽视,它影响地表景观,污染地下水,因此,对生活垃圾渗滤液的处理显得尤为重要。本文采用电化学氧化法研究生活垃圾渗滤液中CODcr和NH3-N去除率的影响因素,通过... 详细信息

目前,生活垃圾污染已经日趋严重,而生活垃圾渗滤液对环境造成的危害更是不容忽视,它影响地表景观,污染地下水,因此,对生活垃圾渗滤液的处理显得尤为重要。本文采用电化学氧化法研究生活垃圾渗滤液中CODcr和NH3-N去除率的影响因素,通过正交实验法得出:当电流密度为7.5A/dm2、NaCl投加量为2g、pH为4时,经过2h的电解,二者的去除率均很高,因此,这种电化学氧化法对生活垃圾渗滤液处理具有较好的效果。

关键词：垃圾渗滤液电化学氧化法正交实验

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P-MSC-derived extracellular vesicles facilitate diabetic wound healing via miR-145-5p/CDKN1A-mediated functional improvements of high glucose-induced senescent fibroblasts

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Burns & Trauma 2023年第1期11卷 325-348页

作者： Jianlong Su Qian Wei Kui ma Yaxi Wang Wenzhi Hu Hao Meng Qiankun Li Yuehou Zhang Wenhua Zhang Haihong Li Xiaobing Fu cuiping Zhang Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division and the 4th Medical Center of Chinese PLA General Hospital 51 Fucheng RoadHaidian DistrictBeijing 100048China School of Medicine NanKai University94Weijin RoadNankai DistrictTianjin 300071China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU05151 Fucheng RoadHaidian DistrictBeijing 100048China Burn and Plastic Surgery Zhongda Hospital Affiliated Southeast UniversityDingjiaqiao 87Gulou DistrictNanjing 210009China Department of Wound Repair Institute of Wound Repair and Regeneration MedicineSouthern University of Science and Technology HospitalSouthern University of Science and Technology School of Medicine6019 Xililiuxian RoadNanshan DistrictShenzhen 518055China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and Regeneration51 Fucheng RoadHaidian DistrictBeijing 100048China

Background:Persistent hyperglycaemia in diabetes causes functional abnormalities of human dermal fibroblasts(HDFs),partially leading to delayed skin wound *** vesicles(EVs)containing multiple pro-healing microRNAs(miRNAs)have been shown to exert therapeutic effects on diabetic wound *** present study aimed to observe the effects of EVs derived from placental mesenchymal stem cells(P-MSC-EVs)on diabetic wound healing and high glucose(HG)-induced senescent fibroblasts and to explore the underlying ***:P-MSC-EVs were isolated by differential ultracentrifugation and locally injected into the full-thickness skin wounds of diabetic mice,to observe the beneficial effects on wound healing in vivo by measuring wound closure rates and histological ***,a series of assays were conducted to evaluate the effects of low(2.28 x 1010 particles/ml)and high(4.56 x 1010 particles/ml)concentrations of P-MSC-EVs on the senescence,proliferation,migration,and apoptosis of HG-induced senescent HDFs in ***,miRNA microarrays and real-time quantitative PCR(RT-qPCR)were carried out to detect the differentially expressed miRNAs in HDFs after EVs *** RNA inhibitors,miRNA mimics,and small interfering RNA(siRNA)were used to evaluate the role of a candidate miRNA and its target genes in P-MSC-EV-induced improvements in the function of HG-induced senescent ***:Local injection of P-MSC-EVs into diabetic wounds accelerated wound closure and reduced scar widths,with better-organized collagen deposition and decreased p16INK4a *** vitro,P-MSC-EVs enhanced the antisenescence,proliferation,migration,and antiapoptotic abilities of HG-induced senescent fibroblasts in a dose-dependent ***-145-5p was found to be highly enriched in ***-145-5p inhibitors effectively attenuated the P-MSC-EV-induced functional improvements of senescent ***-145-5p mimics simulated the effects of P-MSC-EVs on funct

关键词： Diabetic wounds Mesenchymal stem cells Extracellular vesicles miR-145-5p CDKN1A Senescence Fibroblast Glucose

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MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1224-1236页

作者： Qiankun Li Wenzhi Hu Qilin Huang Jie Yang Bingmin Li Kui ma Qian Wei Yaxi Wang Jianlong Su Mengli Sun Shengnan Cui Rungong Yang Haihong Li Xiaobing Fu cuiping Zhang Department of Tissue Repair and Regeneration The First Medical CenterChinese PLA General HospitalBeijing 100853China Research Center for Tissue Repair and Regeneration Medical Innovation Research Department and the Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Dermatology Department The Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationBeijing 100048China School of Medicine NanKai UniversityTianjing 300071China Department of Wound Repair and Dermatologic Surgery Taihe HospitalHubei University of MedicineShiyan 442000China

Unhealable diabetic wounds need to be addressed with the help of newer,more efficacious *** combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound ***,the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch(SFP)were demonstrated to promote diabetic wound *** fibroin binding peptide(SFBP)was screened through phage display,and SFBP-Gluc-MS2(SGM)and pac-miR146a-pac fusion protein were *** designed exosomes(SGM-Exos,miR146a-Exos,and SGM-miR146a-Exos)were isolated from the engineered placental mesenchymal stem cells(PMSCs)transduced with SGM or/and pac-miR146a-pac *** signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of ***,the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in *** to untreated,SGM-miR146a-Exo-only treated,and SFP-only treated groups,SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation,collagen deposition,and *** transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP ***,we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering.

关键词： miR146a wound healing

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Effect of variations in the polar and azimuthal angles of coarse particles on the structure of drainage channels in thickened beds

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International Journal of Minerals,Metallurgy and materials 2023年第12期30卷 2321-2333页

作者： cuiping Li Gezhong Chen Zhu’en Ruan Raimund Bürger Yuan Gao Hezi Hou Hui Wang Key Laboratory of the Ministry of Education of China for High-efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China Shunde Innovation School University of Science and Technology BeijingFoshan 528399China CI2ma and Departamento de Ingeniería matemática Facultad de Ciencias Físicas y MatematicasUniversidad de ConcepcionCasilla 160-CConcepciónChile School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China School of Civil and Resource Engineering University of Science and Technology BeijingBeijing 100083China

The 3D reconstruction and quantitative characterization of drainage channels and coarse tailings particles in a bed were conducted in this *** influence of variations in the azimuthal angle(θ)and polar angle(φ)of coarse particles on drainage channel structure was analyzed,and the drainage mechanism of the bed was *** showed that water discharge in the bed reduced the size of pores and throat channels,increasing slurry *** throat channel structure was a key component of the drainage ***φandθof particles changed predominantly along the length *** changes inφhad a cumulative plugging effect on the drainage channel and increased the difficulty of water *** rake and rod formed a shear ring in the tailings bed with shear,and theθdistribution of particles changed from disorderly to orderly during the rotation *** drainage channel was squeezed during the shearing process with the change inθ,which broke the channel structure,encouraged water discharge in the bed,and facilitated a further increase in slurry *** findings of this work are expected to offer theoretical guidance for preparing high-concentration underflow in the tailings thickening process.

关键词： tailings thickening coarse particle azimuthal angle polar angle drainage channels

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Coherent Mn3O4-carbon nanocomposites with enhanced energy-storage capacitance

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Nano Research 2015年第10期8卷 3372-3383页

作者： Chaofeng Liu Huanqiao Song Changkun Zhang Yaguang Liu cuiping Zhang Xihui Nan Guozhong Cao BeijingInstituteofNanoenergyandNanosystems ChineseAcademyofSciencesBeijing100083China DepartmentofmaterialsScienceandEngineering UniversityofWashingtonSeattleWashington98195USA

Nanostructured Mn3O4 was introduced to activated C （AC） by a novel sonochemical reaction, and the resulting nanocomposites were examined as supercapacitor electrodes. The sonication not only catalyzed the redox reaction but also promoted the diffusion of the precursors, causing the formation of coherent nanocomposites with Mn3O4 nanoparticles grown and uniformly distributed inside the mesopores of the AC. In addition, the extreme local condition in the sonochemical synthesis yielded an excessive amount of divalent manganese ions and oxygen vacancies. This novel microstructure endowed the sample with a superior performance, including a specific capacitance of 150 F/g compared with the value of 93 F/g for AC at a charge/discharge rate of 100 ma/g. A Li-ion capacitor delivered an energy density of 68 Wh/kg, compared with 41 Wh/kg for the AC capacitor at a power density of 210 W/kg.

关键词： sonochemical Mn3O4 Li-on capacitor activated C nanocomposite

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