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Acta Pharmacologica Sinica 2004年第9期 16-21页

作者： jian-xun LIU, Wei-hong cong, Li xu, jian-nong WANG Research Center, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China

AIM: To determine the concentration of acetylcholine (ACh) in amyloid beta-protein (Aβ) treated rats and offer a method determining ACh as well. METHODS: A 1-month combination of extrats of ginseng and ginkgo biloba (Naoweikang) ig administration to rats was performed daily after bilateral injection of Aβ1-40 (4 g/L, 1μL for each side) into hippocampus. After

关键词： ginseng ginkgo biloba acetylcholine high pressure liquid chromatography amyloid beta-protein

Clinical outcomes of fertility-sparing treatments in young patients with epithelial ovarian carcinoma

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第10期12卷 787-795页

作者： Jun HU Li-rong ZHU Zhi-qing LIANG Yuan-guang MENG Hong-yan GUO Peng-peng QU Cai-ling MA cong-jian xu Bi-bo YUAN Department of Gynecology Peking University First Hospital Beijing 100034 China Department of Gynecology Southwest Hospital of Third Military Medical University Chongqing 400038 China Department of Gynecology Chinese PLA General Hospital ＆ Chinese PLA Postgraduate Medical School Beijing 100853 China Department of Gynecology Peking University Third Hospital Beijing 100191 China Tianjin Central Obstetrics and Gynecological Hospital Tianjin 300052 China Department of Gynecology Xinjiang Medical University First Teaching Hospital Urumqi 830054 China Shanghai Red House Hospital of Obstetrics and Gynecology Shanghai 200011 China Department of Gynecology Tianjin Medical University General Hospital Tianjin 300052 China

Objective:To assess the clinical outcomes of fertility-sparing treatments in young patients with epithelial ovarian carcinoma (EOC).Methods:A retrospective study of young EOC inpatients (≤40 years old) was performed during January 1994 and December 2010 in eight ***:Data were analyzed from 94 patients treated with fertility-sparing surgery with a median follow-up time of 58.7 *** histologic grade increased,overall survival (OS) and disease-free survival (DFS) of patients receiving fertility-sparing surgery *** staging surgery nor laparoscopy of early stage EOC with conservative surgery had a significant effect on OS or *** menstruation recommenced after chemotherapy in 89% of the fertility-sparing *** pregnancies among twelve patients were achieved by the end of the ***:Fertility-sparing treatment for patients with EOC Stage I Grade 1 could be cautiously considered for young *** surgical procedure and surgical route might not significantly influence the *** chemotherapy is not likely to have an evident impact on ovarian function or fertility in young patients.

关键词： Epithelial ovarian carcinoma Fertility-sparing treatment Overall survival Disease-free survival Pregnancy

Current Status and Recent Advances in Preimplantation Genetic Testing for Structural Rearrangements

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Reproductive and Developmental Medicine 2020年第1期4卷 1-6页

作者： Shuo Zhang Cai-Xia Lei Xiao-Xi Sun cong-jian xu Department of Genetics Shanghai Ji Ai Genetics and IVF InstituteObstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China

Preimplantation genetic testing(PGT)is an early form of prenatal genetic diagnosis,which can identify the abnormal embryos cultured in vitro,allow only transfer of genetically normal embryos,and improve the pregnancy *** recent years,the rapid development of microarrays and next-generation sequencing(NGS)technologies has remarkably accelerated the clinical application of *** particular,a variety of detection methods have emerged and achieved significant progress in PGT for structural rearrangements(PGT-SR).The detection-related abilities of these methods range from the detection of limited chromosome aneuploidy to comprehensive chromosome screening of the whole genome to differentiation of embryos with normal or balanced translocation/inversion *** this study,we reviewed PGT-SR-related detection techniques to provide a better reference for clinical application and *** have also discussed the potential development of novel techniques in the future.

关键词： Chromosome Aneuploidy Inversion Karyotype Preimplantation Genetic Testing Structural Chromosomal Rearrangement Structural Rearrangement Translocation Karyotype

Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation

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Protein & Cell 2024年第7期15卷 512-529页

作者： Kai-Hui Zhang Fei-Fei Zhang Zhi-Ling Zhang Ke-Fei Fang Wen-Xing Sun Na Kong Min Wu Hai-Ou Liu Yan Liu Zhi Li Qing-Qing Cai Yang Wang Quan-Wei Wei Peng-Cheng Lin Yan Lin Wei xu cong-jian xu Yi-Yuan Yuan Shi-Min Zhao The Obstetrics&Gynecology Hospital of Fudan University State Key Laboratory of Genetic EngineeringFudan UniversityShanghai 200090China Shanghai Key Laboratory of Metabolic Remodeling and Children’s Hospital of Fudan UniversityShanghai 200032China School of Life Sciences and Institutes of Biomedical Sciences Fudan UniversityShanghai 200438China Department of Nutrition and Food Hygiene School of Public HealthNantong UniversityNantong 226019China Reproductive Medicine Center The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Shanghai Fifth People’s Hospital of Fudan University Fudan UniversityShanghai 200240China Department of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210014China Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province College of PharmacyQinghai University for NationalitiesXining 810007China Pediatric Research Institute Children’s Hospital Affiliated to Shandong University(Jinan Children’s Hospital)Jinan 250022China

Polycystic ovary syndrome(PCOS)is the leading cause of anovulatory *** understanding of the ovulation drivers hinders PCOS ***,we report that follicle stimulating hormone(FSH)controls follicular fluid(FF)glutamine levels to determine *** ovulation starts from FF-exposing granulosa cell(GC)*** glutamine,which decreases in pre-ovulation porcine FF,elevates in PCOS patients ***-glutamine chow to elevate FF glutamine inhibits mouse GC apoptosis and induces hormonal,metabolic,and morphologic PCOS ***,follicle-development-driving FSH promotes GC glutamine synthesis to elevate FF glutamine,which maintain follicle wall integrity by inhibiting GC apoptosis through inactivating ASK1-JNK apoptotic *** and glutamine inhibit the rapture of cultured murine *** removal or ASK1-JNK pathway activation with metformin or AT-101 reversed PCOS traits in PCOS models that are induced with either glutamine or *** suggest that glutamine,FSH,and ASK1-JNK pathway are targetable to alleviate PCOS.

关键词： ovulation glutamine granulosa cells PCOS FSH

Liquiritin ameliorates metabolic and endocrine alterations in a mouse model of polycystic ovary syndrome

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Reproductive and Developmental Medicine 2022年第2期6卷 104-112页

作者： Zhi Li Yan Liu Hai-Ou Liu Zhi-Yong Wu cong-jian xu Fei-Fei Zhang Obstetrics and Gynecology Hospital Fudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011China Department of Obstetrics and Gynecology Shanghai Medical CollegeFudan UniversityShanghai 200032China

Objective::Altered bile acid transformation induces low-grade chronic inflammation and may play an important role in the pathophysiology of polycystic ovary syndrome (PCOS). Liquiritincan regulate bile acid metabolism and anti-inflammatory properties;however, limited information is available regarding its therapeutic potential in ***::Female C57BL/6 mice were randomly assigned into four groups ( n = 6 mice/group): the control, letrozole or dehydroepiandrosterone-induced PCOS groups, PCOS + 20 mg/kg liquiritin group, and control + liquiritin groups. After 21 days of treatment, the mice were euthanized, and the associated metabolism indications were investigated. Ovarian histological examinations were performed, and serum hormone concentration was measured. The expression of key genes involved in steroid hormone synthesis, ovarian follicle development, and ovulation was assessed. Results::Liquiritin reduced fasting blood glucose levels and increased insulin sensitivity compared to the PCOS group. Liquiritin also significantly decreased serum levels of total testosterone ( P < 0.001) and dehydroepiandrosterone sulfate ( P < 0.05) in the PCOS group. Histomorphological inspection of ovaries from the liquiritin group revealed fewer cystic dilated follicles than in the PCOS group. Moreover, liquiritinsignificantly ( P < 0.01) decreased Cyp17a1, Cyp19a1, Fshr, Hsd3b2, Runx2, and Ccn2 mRNA expression compared to letrozole-induced PCOS. Conclusion::Liquiritin may be safe and helpful in ameliorating PCOS-associated hyperandrogenemia and hyperglycemia. However, clinical trials investigating different liquiritin dosages are needed to confirm these findings.

关键词： Liquiritin Metabolic phenotypes Polycystic ovary syndrome Reproductive phenotypes

Dysbiosis of Gut Microbiota Contributes to Chronic Stress in Endometriosis Patients via Activating Inflammatory Pathway

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Reproductive and Developmental Medicine 2017年第4期1卷 221-227,I0001页

作者： Jing xu Ke Li Lin Zhang Qi-Yu Liu Yun-Ke Huang Yu Kang cong-jian xu Department of Obstetrics and Gynecology Shanghai Medical SchoolFudan UniversityShanghai 200032China Department of Integrated Traditional Chinese and Western Medicine Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011China

Background:Gut microbiota can interact with the central nervous system through the gut-brain axis,thus affecting the host’s chronic stress level,such as anxiety and *** researches show that patients with endometriosis often have a high level of chronic stress,which will in turn aggravate endometriosis by activating theβ-adrenergic signaling ***,we wondered whether the gut microbiota associates with the chronic stress level in endometriosis patients,which may provide new insights on how to improve ***:We grouped the endometriosis patients into the chronic stress group and the control group with questionnaires such as generalized anxiety disorder-7 and patient health questionnaire-9 and collected patients’fecal specimens and tissue *** microbiota compositions were analyzed by the 16SrRNA gene sequencing-based method,and immunohistochemistry was performed to detect the activation of inflammatory pathways in endometriosis ***:We found that in patients with endometriosis,the dysbiosis of gut microbiota was associated with their stress ***,the levels of Paraprevotella,Odoribacter,Veillonella,and Ruminococcus were significantly reduced in chronic stressed endometriosis patients,suggestive of a disease-specific change of gut microbiota at the genus *** to the healthy women,the expression levels of inflammatory cytokines,nuclear factor-κB p65,and cyclooxygenase-2 increased in the chronic stressed endometriosis patients,indicating that the dysbiosis of gut microbiota may activate the inflammatory pathway of gut-brain ***:We hypothesized that these new disease-specific changes of gut microbiota in chronic stressed patients with endometriosis may be a new examination target of chronic stress *** changes may also provide new insights for psychological intervention,thus reducing the stress level and improving the prognosis of endometriosis patients.

关键词： Chronic Stress Endometriosis Gut Microbiota Gut-brain Axis

A Defective CXCL16/CXCR6 Axis Increases the Risk of Pregnancy Loss via the Abnormal Crosstalk between Decidual γδ T Cells and Trophoblasts

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Reproductive and Developmental Medicine 2021年第3期5卷 129-139页

作者： Deng-xuan Fan Ming-Qing Li Wen-Jie Zhou Hong-Lan Huang Hui-Li Yang cong-jian xu Department of Gynecology Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Department of Obstetrics and Gynecology of Shanghai Medical School Fudan UniversityShanghai200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Fudan UniversityShanghai200011China NHC Key Lab of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research) Hospital of Obstetrics and GynecologyFudan UniversityShanghai200080China Department of Obstetrics and Gynecology Reproductive Medical CenterRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai200025China

Objective::The maternal-fetal interface undergoes dynamic changes to allow the fetus to grow and develop in the *** interaction between decidualγδT cells and trophoblasts plays a pivotal role during successful pregnancy;however,their physiological functions in early-term human pregnancy are still not completely *** study was undertaken to illustrate the functional roles of CXCL16/CXCR6 to prevent pregnancy loss via the crosstalk between decidualγδT cells and HTR8/SVneo trophoblast ***::The percentile of CXCR6+γδT cells in the peripheral blood from normal female and recurrent spontaneous abortion(RSA)patients was analyzed by flow *** expression of CXCR6 was detected in decidual immune cells via flow cytometry,and the expression of CXCL16 was analyzed in HTR8/SVneo trophoblast cells and lentivirus(LV)-HTR8/SVneo trophoblast cells via enzyme-linked immunosorbent *** transcriptase-polymerase chain reaction was used to verify the expression of the CXCL16 gene in LV-HTR8/SVneo trophoblast *** of granzyme B and cytokines and proliferation of decidualγδT cocultured with HTR8/SVneo trophoblast cells were analyzed by flow *** of HTR8/SVneo trophoblast cells was assessed via Matrigel transwell *** transfer was induced in vivo further to illustrate that the normal expression of CXCL16/CXCR6 could prevent pregnancy ***::The percentile of CXCR6+γδT cells in the peripheral blood from RSA patients was lower than normal *** expression of CXCR6 was highest in the decidualγδT cells among decidual immune cells,and the expression of CXCL16 increased as the amount of HTR8/SVneo trophoblast cells *** of granzyme B in the decidualγδT cells was downregulated by cocultured with HTR8/SVneo cells dependent of CXCL16,and HTR8/SVneo trophoblast cells induced the Th2 cytokines production in the decidualγδT *** the expression of CXCR6 in the decidualγδT cells and pr

关键词： CXCL16 CXCR6 DecidualγδT Cells Maternal-fetal Interface Recurrent Spontaneous Abortion Trophoblasts

Overview of in-situ oxygen production technologies for lunar resources

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 233-255页

作者： Youpeng xu Sheng Pang Liangwei cong Guoyu Qian Dong Wang Laishi Li Yusheng Wu Zhi Wang College of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China CAS Key Laboratory of Green Process and Engineering National Engineering Research Center for Green Recycling of Strategic Metal ResourcesInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China

The rich resources and unique environment of the Moon make it an ideal location for human expansion and the utilization of extraterrestrial ***,crucial for supporting human life on the Moon,can be extracted from lunar regolith,which is highly rich in oxygen and contains polymetallic *** oxygen and metal extraction can be achieved using existing metallurgical ***,the ample reserves of water ice on the Moon offer another means for oxygen *** paper offers a detailed overview of the leading technologies for achieving oxygen production on the Moon,drawing from an analysis of lunar resources and environmental *** delves into the principles,processes,advantages,and drawbacks of water-ice electrolysis,two-step oxygen production from lunar regolith,and one-step oxygen production from lunar *** two-step methods involve hydrogen reduction,carbothermal reduction,and hydrometallurgy,while the one-step methods encompass fluorination/chlorination,high-temperature decomposition,molten salt electrolysis,and molten regolith electrolysis(MOE).Following a thorough comparison of raw materials,equipment,technology,and economic viability,MOE is identified as the most promising approach for future in-situ oxygen production on the *** the corrosion characteristics of molten lunar regolith at high temperatures,along with the Moon's low-gravity environment,the development of inexpensive and stable inert anodes and electrolysis devices that can easily collect oxygen is critical for promoting MOE technology on the *** review significantly contributes to our understanding of in-situ oxygen production technologies on the Moon and supports upcoming lunar exploration initiatives.

关键词： lunar resources in-situ oxygen production space metallurgy molten lunar regolith electrolysis

How fertility preservation guidelines have progressed worldwide:Potential implications and inspiration

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Reproductive and Developmental Medicine 2022年第1期6卷 34-41页

作者： Min-Xin Chen Ying Zhang Qing-Qing Cai Shuo Zhang Ran xu Hua Chen Yu Kang Yue Wu Ri-Cheng Chian cong-jian xu President Office Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200090China Fertility Preservation Center Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200090China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200090China Department of Genetics Shanghai JIAI Genetics and IVF InstituteShanghai 200011China Department of International Law China Foreign Affairs UniversityBeijing 100032China Department of Infertility Shanghai JIAI Genetics and IVF InstituteShanghai 200011China Clinical Research Center Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200090China Center for Reproductive Medicine Shanghai 10th People's Hospital of Tongji UniversityShanghai 200072China

To summarize fertility preservation (FP) guidelines and consensus provided by medical facilities worldwide. Five reference databases (PubMed/Embase, China BioMedical Literature Database, ClinicalKey, Wanfang Data, and China Knowledge Resource Integrated) and 7 guideline databases (Guideline International Network, National Collaborating Centre for Women’s and Children’s Health, National Guideline Clearinghouse, The National Institute for Health and Care Excellence, Scottish Intercollegiate Guidelines Network, New Zealand Guideline Group, and Canadian Medical Association infobase) were searched. Official websites of societies were searched to find relevant guidelines and consensus, and guidelines and published systematic reviews and consensus were reviewed from inception through May 2021. A literature search was performed regarding guidance offered in professional facilities, and 304 records (including 4 in Chinese) of existing guidelines for full-text review were found. Moreover, 55 guidelines were identified after a manual search. The literature review demonstrated that FP guidance and options were unevenly distributed worldwide for women who wanted to post-pone pregnancy or had impaired fertility after gonadal toxicity. With promising cancer survival rates and diversified family decisions, more attention should be paid to the improvement and update of accessible guidelines and regulatory infrastructure to inform patients about the available options and empower them to make informed choices. Restrictions to such services can be gradually eased due to the efficacy and safety of certain FP techniques for the right candidates to initiate pregnancy conception.

关键词： Fertility preservation Cancer patient Aging Guidance

Baicalein Ameliorates Chronic Stress-Mediated Ovarian Dysfunction by Upregulating the Expression of Gamma-Aminobutyric Acid B2 Receptor

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Reproductive and Developmental Medicine 2018年第1期2卷 21-29页

作者： Ke Li Jing Li Jing xu Ling Zhang Qi-Yu Liu Yun-Ke Huang Zhi-Gang Zhang Yu Kang cong-jian xu Obstetrics and Gynecology Hospital Fudan UniversityShanghai 200011China State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteRen Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200240China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011China

Background:The aim of this study is to assess the effect of baicalein on chronic stress-mediated ovarian dysfunction in a mouse ***:Forty female C57BL/6 mice were randomly divided into four groups as follows:the normal saline group(control,n=10),the daily stress group(daily stress,n=10),the baicalein group(baicalein,n=10),and the daily stress+baicalein group(daily stress+baicalein,n=10).For the daily stress model,we used a restricted stress ***(10 mg/kg)was administered by gavage every day,and control mice received normal saline *** specimens were harvested after 4 *** of norepinephrine(NE)in serum was performed to assess the psychological stress level of the *** addition,histological changes of the uterus and ovaries and the levels of anti-Müllerian hormone(AMH)in serum were assessed to evaluate changes in ovarian *** detect the underlying mechanisms of the amelioration of baicalein in chronic stress-mediated ovarian dysfunction,immunohistochemical methods,and quantitative real-time polymerase chain reaction were applied to determine the expression of gamma-aminobutyric acid(GABA)***:Compared with values in the control group,serum NE concentrations were significantly increased(P<0.001),AMH concentrations were markedly decreased(P<0.01),the thickness of the endometrium was clearly reduced,and the percentage of atretic follicles was significantly increased in the daily stress group(P<0.001),indicating that the chronic stress model was successfully *** contrast,compared with values in the daily stress group,serum NE concentrations were significantly reduced(P<0.001),AMH concentrations were significantly enhanced(P<0.05),the thickness of the endometrium was clearly increased,and the percentage of atretic follicles was significantly reduced(P<0.001)in the daily stress+baicalein group,indicating that baicalein clearly attenuated the ovarian dysfunction mediated by chronic ***

关键词： Baicalein Chronic Stress Gamma‑Aminobutyric Acid B2 Receptor Ovarian Dysfunction