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Progress in the development of techniques based on light scattering for single nanoparticle detection

Science China chemistry 2011年第8期54卷 1244-1253页

作者： ZHU ShaoBin, WANG Shuo, YANG LingLing, HUANG TianXun & YAN XiaoMei Department of Chemical biology, college of chemistry and Chemical engineering Key Laboratory for Chemical biology of Fujian Province Key Laboratory of Analytical Science, Xiamen University, Xiamen 361005, China 1. Department of Chemical biology College of Chemistry and Chemical Engineering Key Laboratory for Chemical biology of Fujian Province Key Laboratory of Analytical Science Xiamen University Xiamen 361005 China

Nanoparticles have recendy attracted extensive attention in view of their great potential in biomedicine and bioanalytical applications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparficles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles.

关键词： light scattering single particle detection nanoparticles confocal correlation spectroscopy flow cytometry

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AlkB recognition of a bulky DNA base adduct stabilized by chemical cross-linking

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Science China chemistry 2010年第1期53卷 86-90页

作者： YI ChengQi1 & HE Chuan1,2 1Department of chemistry,University of Chicago,Chicago,Illinois 60637,USA 2Department of Chemical biology,college of chemistry and Molecular engineering,Peking University,Beijing 100871,China 1. Department of chemistry University of Chicago Chicago Illinois 60637 USA2. Department of Chemical Biology College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

*** AlkB is a direct DNA/RNA repair protein that oxidatively reverses N1 alkylated purines and N3 alkylated pyrimidines to regular *** crystal structures have revealed N1-methyl adenine(1-meA) recognition by AlkB and a unique base flipping mechanism,but how the AlkB active site can accommodate bulky base adducts is largely *** a previously developed chemical cross-linking technique,we crystallized AlkB with a duplex DNA containing a caged thymine base(cagedT).The structure revealed a flexible hairpin lid and a reorganized substrate recognition loop used by AlkB to accommodate *** observations demonstrate,at the molecular level,how bulky DNA adducts may be recognized and processed by AlkB.

关键词： DNA/RNA repair AlkB caged thymine disulfide cross-linking bulky base adduct

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DESCRIPTION OF TWO NEW SPECIES OF HALIOTREMA JOHNSTON ＆TIEGS （MONOGENEA, ANCYROCEPHALIDAE）

DESCRIPTION OF TWO NEW SPECIES OF HALIOTREMA JOHNSTON ＆TIEG...

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第十九届国际动物学大会

作者： Hai-Yan LiDepartment of biology college of biology & chemistry engineering Guangzhou University Guangzhou 510405 China

＜正＞Two new species of monogenegs were collected: Haliotrema neokurodai, sp. nov. ＆ H. flexiosus , ***. from the gills of a marine eleoridid, Sparus macrocephalus （Basilewsdy）. They are attributed to the genus Ha...

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Enantioselective hydrogenation of ethyl pyruvate catalyzed by carbon-nanotubes supported rhodium nanoclusters

Enantioselective hydrogenation of ethyl pyruvate catalyzed b...

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第十届中俄双边新材料新工艺研讨会

作者： MA Hongxia,BAO Jiqing,TAN Jun,and GU Li college of biology and chemistry engineering,Jiaxing University,Jiaxing 314001,China

The heterogeneous enantioselective hydrogenation of ethyl pyruvate in the presence of cinchonidine modified Rhodium nano-clusters was *** results show that the reaction conditions,such as solvent,modification concentration,tempreture,hydrogen pressure and reaction time can influence this enantioselective *** the optimum conditions,293 K,7.0 MPa of hydrogen pressure and 4.0×10 mol/L of cinchonidine in tetrahydrofuran,the mole conversion of ethyl pyruvate and enantiomeric excess of R-ethyl lactate reach up to 100%and 62.6%,respectively.

关键词： rhodium nanociusters carbon-nanotube cinchonidine ethyl pyruvate enantioselective hydrogenation

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A General Route to Synthesis of 1-Substituted Taurines

A General Route to Synthesis of 1-Substituted Taurines

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第三届全国有机化学学术会议

作者： XU, Jia-Xi XU, Shu ZHNAG, Qi-Han ( Key Laboratory of Bioorganic chemistry and Molecular engineering of Ministry of Education , Department of Chemical biology, college of chemistry and Molecular engineering, Peking University, Beijing 100871)

＜正＞ Several 2-aminoalkanesulfonic acids have been found in many mammalian tissues till now and are involved in various and important physiological processes. Recently attention has been paid to their syntheses for studies of these physiological processes, the structural modification of natural peptides to overcome the limitations associated with their development as therapeutically useful agents, and information on the nature and mechanism of the peptide-re ceptor interactions.β-Aminoalkanesulfonic acids are sulfur analogues of naturally occurring amino acids because α-aminoalkanesul fonic acids and their derivatives are unstable. There are two types of β-aminoalkanesulfonic acids, 1-alkyltaurines

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Direct Synthesis of Phosphonopeptides via Mannich-Type Four-Component Condensation

Direct Synthesis of Phosphonopeptides via Mannich-Type Four-...

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8th Chinese International Peptide Symposium

作者： Jiaxi XU, Nanyan FU, Yuanhe GAO, Qihan ZHANG Key Laboratory of Bioorganic chemistry and Molecular engineering of Ministry of Education, Department of Chemical biology, college of chemistry and Molecular engineering, Peking University, Beijing 100871, China

Phosphonopeptides are important analogs of naturally occurring peptides and are widely used as enzyme inhibitors and haptens for the induction of catalytic antibodies. Phosphonopeptides were prepared traditionally from aminoalkylphosphonochloridates and amino/hydroxy esters or peptide esters. An alternative route to synthesize phosphonopeptides involves nucleophilic addition to much more reactive phoshonochloridites and followed by oxida

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The Involvement of Lipid Rafts in Epidermal Growth Factor-induced Chemotaxis of Breast Cancer Cells

The Involvement of Lipid Rafts in Epidermal Growth Factor-in...

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第五届全国化学生物学学术会议

作者： YING LIU NING ZHANG Beijing National Laboratory for Molecular Sciences(BNLMS)and Department of Chemical biology,college of chemistry and Molecular engineering,Peking University,Beijing 100871,China.

＜正＞Metastasis is the major cause of morbidity and mortality in *** studies reveal a role of chemotaxis in cancer cell *** growth factor receptors（EGFR）have potent chemotactic effects on human breast cancer *** rafts,organized microdomain on plasma membranes,regulate the activation of many membrane *** the current study,we investigated the role of lipid rafts in EGFR-mediated cancer cell *** confocal

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The discovery of the mini-protease:Seryl Histidine Dipeptide

The discovery of the mini-protease:Seryl Histidine Dipeptide

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中国化学会第26届学术年会化学生物分会场

作者： Xiao-xia Liu, Yan Liu, Ming-Kun Lin, Yu-fen Zhao* The Key Laboratory for Chemical biology of Fujian Province, Department of Chemical biology, college of chemistry and Chemical engineering, Xiamen University, Xiamen 361005

Our previous researches showed that Seryl-Histidine dipeptide (Ser-His) could cleave proteins such as bovine serum albumen (BSA) [1] and green fluorescence protein (GFP) [4] over wide ranges of pH and temperature, as well as DNA [1], with a hydrolysis mechanism. Therefore, Ser-His is the shortest peptide with the hydrolysis cleavage activity on protein, and its function is similar to serine protease. Recently, the discovery of extended substrate specificity of opossum chymase illuminated the origin of mast cell chymases [5, 6]. So, to investigate the function of Ser-His further might provide more information to understand the function group evolution for the origin of serine protease. In this paper, high resolution mass spectrometry was applied to study the cleavage sites of Seryl-Histidine dipeptide (Ser-His) on two different proteins, namely green fluorescence proteins (xm18) and cyclophilin A. It was found that the two different protein, were both cleaved by Ser-His into small polypeptide fragments. Through comparison of the cleavage activity of Ser-His and Protease K, it means that the hydrolysis activity of Ser-His is about one thirty thousandths of that of Protease K. Although Ser-His possesses non-specificity cleavage activity on the primary sequence of substrate proteins, but, amino acid residue, F, is the more favorite cleavage site (Fig. 1 and Fig. 2), which is similar to the natural serine protease, chymotrypsin. Therefore, the smallest peptide, Ser-His dipeptdie, was named the as mini-protease. It implied that Ser-His is probably the origin of serine proteases.

关键词： Ser-His diepetide mini-protease cleavage selectivity evolutionary origin

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Probing biological Process via Single Molecule Detection

Probing biological Process via Single Molecule Detection

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第五届全国化学生物学学术会议

作者： Xin Sheng Zhao Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural chemistry of Unstable and Stable Species,and Department of Chemical biology,college of chemistry and Molecular engineering,Peking University,Beijing 100871,China.

＜正＞Single molecule detection（SMD）emerges as a powerful tool to investigate biological process in vitro and in *** have applied SMD to study the dynamics of DNA hybridization,protein

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Novel and Convenient Method for the Preparation of Phosphonate Peptides and Phosphonamidate Peptides

Novel and Convenient Method for the Preparation of Phosphona...

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第三届全国有机化学学术会议

作者： XU, Jia-Xi FU, Nan-Yan GAO, Yuan-He ZHNAG, Qi-Han DUAN, Li-Fang (Key Laboratory of Bioorganic chemistry and Molecular engineering of Ministry of Education, Department of Chemical biology, college of chemistry and Molecular engineering, Peking University, Beijing 100871, China)

＜正＞ Phosphonate and phosphonamidate peptides are phosphorus analogues of natural peptides. They have been great used as stable mimetics of tetrahedral transition states as enzyme inhibitors and as haptens for catalytic antibody research in recent years. Although several methods are available for the preparation of phosphonate peptides and phosphonamidate peptides, all of them use phosphonic acid derivatives as starting materials. The overall yields from the synthesis of phosphonic acid derivatives to desired peptides are not satisfactory in most cases.A novel and direct method for the preparation of phosphonate mixed diesters and phosphonamidates was devel

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