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Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds

Science China Chemistry 2010年第5期53卷 1010-1016页

作者： BASTON David S. KHAN Elaine SORRENTINO claudio DENISON Michael S. Laboratorium voor Analytische en Milieu Chemie Vrije Universiteit Brussel Pesticide Epidemiology Section Pesticide and Environmental Toxicology BranchOffice of Environmental Health Hazard Assessment Human and Ecological Risk Office Brownfields and Environmental Restoration ProgramDepartment of Toxic Substances Control Department of Environmental Toxicology University of California

Reporter genes produce a protein product in transfected cells that can be easily measured in intact or lysed cells and they have been extensively used in numerous basic and applied research *** the past 10 years,reporter gene assays have been widely accepted and used for analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like compounds in various types of matrices,such as biological,environmental,food and feed samples,given that high-resolution instrumental analysis techniques are impractical for large-scale screening *** most sensitive cell-based reporter gene bioassay systems developed are the mechanism-based CALUX(Chemically Activated Luciferase Expression) and CAFLUX(Chemically Activated Fluorescent Expression) bioassays,which utilize recombinant cell lines containing stably transfected dioxin(AhR) responsive firefly luciferase or enhanced green fluorescent protein(EGFP) reporter genes,*** the current CALUX and CAFLUX bioassays are very sensitive,increasing their lower limit of sensitivity,magnitude of response and dynamic range for chemical detection would significantly increase their utility,particularly for those samples that contain low levels of dioxin-like HAHs(i.e.,serum) .In this study,we report that the addition of modulators of cell signaling pathways or modification of cell culture conditions results in significant improvement in the magnitude and overall responsiveness of the existing CALUX and CAFLUX cell bioassays.

关键词： dioxin reporter gene bioassay CALUX CAFLUX

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Selective Gold Deposition on a Nanostructured Block Copolymer Film Crystallized by Epitaxy

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Nano Research 2011年第3期4卷 241-248页

作者： claudio De Rosa Finizia Auriemma Rocco Di Girolamo Rossella Aprea Annette Thierry Dipartimento di Chimica ＂P. Corradini＂ Universit~t di Napoli ＂Federico II＂ Complesso Monte S. Angelo Via Cintia 80126 Napoli Italy Institut Charles Sadron University de Strasbourg CNRS-UPR 22 B. E 84047 23 rue du Loess 67034 Strasbourg France

An ordered nanostructure formed by epitaxial crystallization of a semicrystalline block copolymer on a substrate has been used as a patterned template for the selective deposition of thermally evaporated gold nanoparticles, resulting in the formation of structure-guiding host nanocomposites in which the ordered distribution of the guest particles is guided by the ordering of the host nanostructured block copolymer matrix. This opens new perspectives in the field of polymeric composites related to the maximum enhancement of effective physical properties and to the numerous possible applications that arise due to the presence of long-range order in the spatial distribution of functional nanoparticles.

关键词： Crystalline block copolymers nanostructures nanocomposites gold deposition

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Evolution of personality and locomotory performance traits during a late Pleistocene island colonization in a tree frog

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Current Zoology 2023年第5期69卷 631-641页

作者： Roberta Bisconti claudio Carere David Costantini Anita Liparoto Andrea Chiocchio Daniele Canestrelli Department of Ecological and Biological Science Tuscia UniversityLargo dell’Universitàs.n.c.01100 ViterboItaly UnitéPhysiologie Moléculaire et Adaptation(PhyMA) Muséum National d’Histoire NaturelleCNRSCP3257 rue Cuvier 75005 ParisFrance

Recent empirical and theoretical studies suggest that personality and locomotory performance traits linked to dispersal abilities are crucial components of the dispersal syndromes,and that they can evolve during range expansions and colonization *** colonization is one of the best characterized processes in dispersal biogeography,and its implication in the evolution of phenotypic traits has been investigated over a wide range of temporal ***,the effect of island colonization on personality and performance traits of natural populations,and how these traits could drive island colonization,has been little ***,no studies have addressed these processes in the context of late Pleistocene range ***,we investigated the contribution of island colonization triggered by postglacial range expansions to intraspecific variation in personality and locomotory performance *** compared boldness,exploration,jumping performance,and stickiness abilities in populations from 3 equidistant areas of the Tyrrhenian tree frog Hyla sarda,2 from the main island(Corsica Island),and 1 from the recently colonized island of *** from Elba were significantly bolder than individuals from Corsica,as they emerged sooner from a shelter(P=0.028),while individuals from Corsica showed markedly higher jumping and stickiness performance(both P<0.001),resulting as more performing than those of *** discuss these results in the context of the major microevolutionary processes at play during range expansion,including selection,spatial sorting,founder effects,and their possible interaction with local adaptation processes.

关键词： behavioral syndrome colonization dispersal founder effect Hyla sarda insularity personality

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Immunotherapy against programmed death-1/programmed death ligand 1 in hepatocellular carcinoma: Importance of molecular variations, cellular heterogeneity, and cancer stem cells

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World Journal of Stem Cells 2021年第7期13卷 795-824页

作者： Caecilia H C Sukowati Korri Elvanita El-Khobar claudio Tiribelli Centro Studi Fegato Fondazione Italiana Fegato ONLUSTrieste 34149Italy Hepatitis Unit Eijkman Institute for Molecular BiologyJakarta 10430Indonesia

Hepatocellular carcinoma(HCC)is a heterogeneous malignancy related to diverse etiological *** oncogenic mechanisms and genetic variations lead to multiple HCC molecular ***,an immune-based strategy using immune checkpoint inhibitors(ICIs)was presented in HCC therapy,especially with ICIs against the programmed death-1(PD-1)and its ligand ***,despite the success of anti-PD-1/PD-L1 in other cancers,a substantial proportion of HCC patients fail to *** this review,we gather current information on biomarkers of anti-PD-1/PD-L1 treatment and the contribution of HCC heterogeneity and hepatic cancer stem cells(CSCs).Genetic variations of PD-1 and PD-L1 are associated with chronic liver disease and progression to ***-L1 expression in tumoral tissues is differentially expressed in CSCs,particularly in those with a close association with the tumor *** information will be beneficial for the selection of patients and the management of the ICIs against PD-1/PD-L1.

关键词： Hepatocellular carcinoma Programmed death-1 Programmed death ligand 1 Cancer stem cells Cancer heterogeneity Genetic variants

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Diet and microbiota in inflammatory bowel disease: The gut in disharmony

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World Journal of Gastroenterology 2017年第12期23卷 2124-2140页

作者： Davy CM Rapozo claudio Bernardazzi Heitor Siffert Pereira de Souza Coordenacao de Pesquisa Instituto Nacional de Cancer Servico de Gastroenterologia e Laboratório Multidisciplinar de Pesquisa Departamento de Clínica MédicaHospital Universitário Clementino Fraga FilhoUniversidade Federal do Rio de Janeiro D’Or Institute for Research and Education Rua Diniz Cordeiro 30BotafogoRio de JaneiroRJ 22281-100Brazil

Bacterial colonization of the gut shapes both the local and the systemic immune response and is implicated in the modulation of immunity in both healthy and disease states. Recently, quantitative and qualitative changes in the composition of the gut microbiota have been detected in Crohn's disease and ulcerative colitis, reinforcing the hypothesis of dysbiosis as a relevant mechanism underlying inflammatory bowel disease(IBD) pathogenesis. Humans and microbes have coexisted and co-evolved for a long time in a mutually beneficial symbiotic association essential for maintaining homeostasis. However, the microbiome is dynamic, changing with age and in response to environmental modifications. Among such environmental factors, food and alimentary habits, progressively altered in modern societies, appear to be critical modulators of the microbiota, contributing to or co-participating in dysbiosis. In addition, food constituents such as micronutrients are important regulators of mucosal immunity, with direct or indirect effects on the gut microbiota. Moreover, food constituents have recently been shown to modulate epigenetic mechanisms, which can result in increased risk for the development and progression of IBD. Therefore, it is likely that a better understanding of the role of different food components in intestinal homeostasis and the resident microbiota will be essential for unravelling the complex molecular basis of the epigenetic, genetic and environment interactions underlying IBD pathogenesis as well as for offering dietary interventions with minimal side effects.

关键词： Diet Microbiota Epigenetics Crohn’s disease Ulcerative colitis

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Gastrointestinal and nutritional care in pediatric neuromuscular disorders

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世界临床儿科杂志 2023年第4期12卷 197-204页

作者： Valeria Dipasquale Rossella Morello claudio Romano Department of Human Pathology in Adulthood and Childhood“G.Barresi” University Hospital“G.Martino”Messina 98124Italy

Neuromuscular diseases(NMDs)affect the development and growth of the neuromuscular system in *** pathology can occur anywhere along the neuromuscular pathway,from the brain to the nerves to the muscle *** diseases have a profound impact on the quality of life not only of children but also of their *** predominant manifestation in NMDs is hypotonia,which leads to muscle weakness and fatigue,reduced mobility,and decreased physical ***,multiple organ systems can be affected,with resulting orthopedic,cardiac,infectious,respiratory,and nutritional *** with NMD present an increased risk for several dietary and feeding difficulties because of their neuromuscular diagnosis,presentation,and *** problems include chronic gastrointestinal issues(constipation,dysphagia,gastroesophageal reflux,and diarrhea),dysphagia,malnutrition,and body composition *** a result,compared to the overall pediatric population,infants and children with NMD are more likely to be malnourished,ranging from failure to thrive to overweight or ***-specific guidelines vary in level of detail and recommendations for dietary ***,nutritional data available are sparse,with the exception of Duchenne muscular dystrophy,spinal muscular atrophy,and congenital muscular *** purpose of this review is to describe the spectrum of nutritional challenges in children with NMD and to summarize the main dietary and gastrointestinal recommendations for each neuromuscular disorder to provide guidance for daily clinical practice.

关键词： Neuromuscular disorders Diet Malnutrition Overweight Dysphagia Gastrointestinal dysmotility Gastrointestinal symptoms Tube feeding

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Platelet rich plasma(PRP)induces autophagy in osteoblast precursor 3T3-L1

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BIOCELL 2018年第1期42卷 13-16页

作者： SERGIO ANDRES CARMINATI MARÍA CAROLINA BARBOSA claudio MARCELO FADER Universidad Nacional de Cuyo Facultad de OdontologíaMendozaArgentina Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET).Instituto de Histología y Embriología(IHEM) Facultad de Ciencias MédicasUniversidad Nacional de CuyoMendozaArgentina

Autophagy is an essential cellular homeostatic mechanism by which intracellular components are delivered into the lysosomes for degradation and *** has been related with a diversity of pathological or physiological dentary processes such as bone remodeling,skeletal aging,osteoclastogenesis,osteoblastogenesis and different types of oral ***-rich plasma(PRP),isolated from autologous blood,is a plasma preparation containing a higher concentration of platelets which contains numerous different growth factors and cytokines that activate several cellular signaling *** purpose of this study is to investigate the effect of PRP on autophagy stimulation in both osteoblast precursor 3T3-L1 and non-related osteoblastic *** results showed that PRP can increase the number of autophagic structures in 3T3-L1 and HeLa(cervical cancer cells)***,we have determined by Western blot a rise in the lipidated form of the autophagic protein LC3(***3-II)upon PRP *** together,our results suggest that PRP is able to induce a strongly autophagy response in osteoblast precursor and,to a lesser extent,in non-related osteoblastic cells,suggesting that PRP could be a potential therapeutic tool for some autophagy-related diseases associated with bone homeostasis.

关键词： Autophagy 3T3-L1 Platelet rich plasma Odontology

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Impact of healthy plant-based diet on abdominal visceral and liver fat contents

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Hepatobiliary Surgery and Nutrition 2021年第1期10卷 136-138页

作者： Lucia Pacifico Francesco Massimo Perla claudio Chiesa Department of Maternal and Child health Sapienza University of RomeRomeItaly Institute of Translational Pharmacology National Research CouncilRomeItaly

Diet is an important modifiable risk factor for obesity and obesity-related complications,and there has been growing interest in plant-based diets,mainly defined as“vegetarian”diets,which have been associated with a lower prevalence rate of overweight/obesity as well as a lower risk of coronary heart disease,type 2 diabetes,other cardiometabolic diseases,and all-cause mortality(1).Such dietary patterns are also a major determinant of environmental sustainability(1).Of note,however,vegetarian diets can include less healthy plant foods,such as refined grains and sugar-sweetened beverages which have detrimental effects on health(2).

关键词： obesity mortality diseases

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Compromised nutritional status in patients with end-stage liver disease:Role of gut microbiota

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Hepatobiliary & Pancreatic Diseases International 2018年第4期17卷 290-300页

作者： Maria Vasco Rossella Paolillo Concetta Schiano Linda Sommese Oreste Cuomo claudio Napoli U.O.C.Division of Clinical Immunology ImmunohematologyTransfusion Medicine and Transplant ImmunologyRegional Reference Laboratory of Transplant ImmunologyDepartment of Internal and Specialty MedicineAzienda Ospedaliera UniversitariaUniversitàdegli Studi della Campania“Luigi Vanvitelli”Naples 80138Italy IRCCS SDN NaplesItaly Department of Experimental Medicine Universitàdegli Studi della Campania“Luigi Vanvitelli”Naples 80138Italy Department of Liver Transplant AORN A.CardarelliNaplesItaly Department of Medical SurgicalNeurologicalMetabolic and Geriatric SciencesUniversitàdegli Studi della Campania“Luigi Vanvitelli”NaplesItaly

Background: Patients with end-stage liver disease(ESLD) have a compromised nutritional status because of the liver crucial role in regulating metabolic homeostasis and energy *** sources: A systematic review of literature based on extensive relevant articles published from 2001 to 2017 in English in Pub Med database was performed by searching keywords such as liver disease, nonalcoholic liver disease, alcoholic liver disease, malnutrition, epigenetics, gut microbiota, and ***: Liver transplantation would be one eligible therapy for ESLD patients, even if, the clinical outcome is negatively influenced by malnutrition and/or infections. The malnutrition is a condition of nutrient imbalance with a high incidence in ESLD patients. An accurate evaluation of nutritional status could be fundamental for reducing complications and prolonging the survival of ESLD patients including those undergoing liver transplantation. In addition, the interaction among nutrients, diet and genes via epigenetics has emerged as a potential target to reduce the morbidity and mortality in ESLD patients. The malnutrition induces changes in gut microbiota causing dysbiosis with a probable translocation of bacteria and/or pathogen-derived factors from the intestine to the liver. Gut microbiota contribute to the progression of chronic liver diseases as well as hepatocellular carcinoma. The administration of probiotics modulating gut microbiota could improve all chronic liver ***: This review provides an update on malnutrition status linked to epigenetics and the potential benefit of some probiotics on the management of ESLD patients. In support of this view and to reveal the constant and growing interest in this field, some clinical trials are reported.

关键词： Liver diseases Liver transplantation Epigenetics Malnutrition Probiotics

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The Second Edition of the Integrative Levels Classification: Evolution of a KOS

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Journal of Data and Information Science 2020年第1期5卷 39-50页

作者： Ziyoung Park claudio Gnoli Daniele P.Morelli Department of Library&Information Science Hansung UniversitySeoulRepublic of Korea Science and Technology Library University of PaviaPavia27100Italy Kaboom Torino 10138Italy

Purpose:This paper informs about the publication of the second edition of the Integrative Levels Classification(ILC2),a freely-faceted knowledge organization system(KOS),and reviews the main changes that have been introduced as compared to its first edition(ILC1).Design/methodology/approach:The most relevant changes are illustrated,with special reference to those of interest to general classification theory,by means of examples of notation for individual classes and combinations of ***:Changes introduced in ILC2 include:the names and order of some main classes;the development of subclasses for various phenomena,especially quantities and algebraic structures;the order of facet categories and the new category of Disorder;notation for special facets;distinction of the semantical function of facets(attributes)from their syntactic *** system can be freely accessed online through a PHP browser as well as in SKOS *** limitations:Only a selection of changed classes is discussed for space *** implications:ILC1 has been previously applied to the BARTOC directory of *** of BARTOC data to ILC2 and application of ILC2 to further information systems are *** methods for reclassifying BARTOC with ILC2 are ***:ILC is a newly developed classification system,based on phenomena instead of traditional disciplines and featuring various innovative *** paper is an original account of its most recent evolution.

关键词： Freely faceted classification Fundamental categories Knowledge organization system Phenomenon-based classification

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