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A Chemical Approach for Real-time Monitoring Neuronal Activities

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Chemical Research in Chinese Universities 2020年第4期36卷 729-730页

作者： LI Qian fan chunhai School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240P.R.China

In the latest issue of Nature Nanotechnology,Ling and coworkers reported the first potassium nanosensor applicable in a freely moving *** was developed by embedding the mesoporous silica nanoparticle framework with optical potassium indicators and then shielding the framework with an ultrathin layer of a potassium-permeable *** nanosensor achieved highly sensitive monitoring of the dynamic changes of the extracellular potassium in the brain of freely moving mice with epilepsy.

关键词： potassium epilepsy moving

Biomacromolecular nanostructures-based interfacial engineering: from precise assembly to precision biosensing

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National Science Review 2018年第5期5卷 740-755页

作者： fan Yang Xiaolei Zuo chunhai fan Xian-En Zhang Division of Physical Biology & Bioimaging Center Shanghai Synchrotron Radiation FacilityShanghai Institute of Applied PhysicsChinese Academy of Sciences National Key Laboratory of Biomacromolecules CAS Excellence Center for BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences School of Laboratory Medicine Hubei University of Chinese Medicine

Biosensors are a type of important biodevice that integrate biological recognition elements, such as enzyme,antibody and DNA, and physical or chemical transducers, which have revolutionized clinical diagnosis especially under the context of point-of-care tests. Since the performance of a biosensor depends largely on the bio–solid interface, design and engineering of the interface play a pivotal role in developing quality biosensors. Along this line, a number of strategies have been developed to improve the homogeneity of the interface or the precision in regulating the interactions between biomolecules and the interface. Especially,intense efforts have been devoted to controlling the surface chemistry, orientation of immobilization,molecular conformation and packing density of surface-confined biomolecular probes(proteins and nucleic acids). By finely tuning these surface properties, through either gene manipulation or self-assembly, one may reduce the heterogeneity of self-assembled monolayers, increase the accessibility of target molecules and decrease the binding energy barrier to realize high sensitivity and specificity. In this review, we summarize recent progress in interfacial engineering of biosensors with particular focus on the use of protein and DNA nanostructures. These biomacromolecular nanostructures with atomistic precision lead to highly regulated interfacial assemblies at the nanoscale. We further describe the potential use of the high-performance biosensors for precision diagnostics.

关键词： biosensor interface engineering homogeneity orientation control fused proteins DNA nanostructures

Framework Nucleic Acids for Cell Imaging and Therapy

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Chemical Research in Chinese Universities 2020年第1期36卷 1-9页

作者： GE Zhilei LI Qian fan chunhai School of Chemistry and Chemical Engineering and Institute of Molecular MedicineRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200240P.R.China

Over the past decade,structural DNA nanotechnology has been well developed to be a promising and powerful technique to generate various nanostructures with programmability,spatial organization and *** the advent of computer-aided tools,framework nucleic acids have been employed in a series of biomedical applications,ranging from biosensing,bioimaging,diagnosis,to *** this review,we summarized recent advances in the construction of precisely assembled DNA nanostructures,and DNA-engineered *** also outlined the challenges and opportunities for the translational applications of framework nucleic acids.

关键词： DNA nanotechnology Framework nucleic acid DNA origami Therapy Cellular imaging

DNA framework-engineered electrochemical biosensors

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Science China(Life Sciences) 2020年第8期63卷 1130-1141页

作者： fan Li Qian Li Xiaolei Zuo chunhai fan Institute of Molecular Medicine Renji HospitalSchools of MedicineShanghai Jiao Tong UniversityShanghai 200127China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various *** particular,by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision,molecular recognition on the electrochemical biosensing interface has been significantly enhanced,leading to the development of highly sensitive and specific biosensors for nucleic acids,small molecules,proteins,and *** this review,we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.

关键词： framework nucleic acids biosensing interface electrochemical biosensors biomarkers DNA

Recent advances in two-dimensional nanomaterials-based electrochemical sensors for environmental analysis

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Green Energy & Environment 2018年第2期3卷 97-106页

作者： Shao Su Shimou Chen chunhai fan Division of Physical Biology Shanghai Institute of Applied PhysicsChinese Academy of Sciences Key Laboratory for Organic Electronics & Information Displays (KLOEID) Institute of Advanced Materials (IAM)Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM)Nanjing University of Posts & Telecommunications Beijing Key Laboratory of Ionic Liquids Clean Process Key Laboratory of Green Process and EngineeringInstitute of Process Engineering (IPE)Chinese Academy of Sciences (CAS)

With the rapidly increased concerns in environmental pollution, there have been urgent needs to develop fast, sensitive, low-cost and multiplexed sensing devices for the detection of environmental pollutants. Two-dimensional(2D) nanomaterials hold great promise due to their unique chemical and physical properties, which have been extensively employed to monitor the environmental pollutants combined with different detection techniques. In this review, we summarize recent advances in 2D nanomaterials-based electrochemical sensors for detecting heavy metal ions, organic compounds, pesticides, antibiotics and bacteria. We also discuss perspectives and challenges of 2D nanomaterials in environmental monitoring.

关键词： Two dimensional Nanomaterials Electrochemical sensor Environmental monitoring

Graphene-based nanomaterials in biosystems

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Nano Research 2019年第2期12卷 247-264页

作者： Na Lu Liqian Wang Min Lv Zisheng Tang chunhai fan School of Materials Engineering Shanghai University of Engineering Science Shanghai 201620 China Division of Physical Biology and Bioimaging Center Shanghai Synchrotron Radiation Facility CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China Department of Endodontics Shanghai Ninth Peoples Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai 200011 China National Clinical Research Center of Oral Diseases Shanghai 200011 China Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology Shanghai 200011 China School of Chemistry and Chemical Engineering and Institute of Molecular Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200240 China

Graphene-based nanomaterials have emerged as a novel type of materials with exceptional physicochemical properties and numerous applications in various areas. In this review, we summarize recent advances in studying interactions between graphene and biosystems. We first provide a brief introduction on graphene and its derivatives, and then discuss on the toxicology and biocompatibility of graphene, including the extracellular interactions between graphene and biomacromolecules, cellular studies of graphene, and in vivo toxicological effects. Next, we focus on various graphene-based practical applications in antibacterial materials, wound addressing, drug delivery, and water *** finally present perspectives on challenges and future developments in these exciting fields.

关键词： graphene-based nanomaterials toxicology and biocompatibility biomacromolecules cells living entitles applications

Size-dependent cellular uptake and sustained drug release of PLGA particles

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Particuology 2023年第2期73卷 1-7页

作者： Rui Sun Xia Liu Yu Zhang Qian Li Ying Zhu chunhai fan Division of Physical Biology CAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China The Interdisciplinary Research Center Shanghai Synchrotron Radiation FacilityZhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China University of Chinese Academy of Sciences Beijing 100049China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational MedicineShanghai Jiao Tong UniversityShanghai 200240China

Poly(lactic-co-glycolic)acid(PLGA)particles have become a commonly used drug delivery strategy in the pharmaceutical *** this work,we aim to investigate the size-dependent cellular internalization of PLGA particles and its effects on sustained drug *** prepared three different-sized particles using PLGA(200,500 and 2000 nm)ranging from submicrometer to ***(DEX)with excellent anti-inflammatory properties was used as a model drug to prepare DEX-loaded PLGA particles(DPs).We comprehensively investigated the encapsulation efficiency,cellular uptake and in vitro drug release *** assessment revealed that,in the lipopolysaccharide(LPS)-induced RAW 264.7 cells model,500 nm DPs showed sustained anti-inflammatory *** work provides important information for designing PLGA-based drug delivery systems for biomedical applications.

关键词： DEX-loaded PLGA particles(DPs) Sustained release Anti-inflammatory efficacy

Robust and efficient direct multiplex amplification method for large-scale DNA detection of blood samples on FTA cards

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Nuclear Science and Techniques 2013年第3期24卷 72-80页

作者： JIANG Bowei XIANG Fawei ZHAO Xingchun WANG Lihua fan chunhai Laboratory of Physical Biology Shanghai Institute of Applied PhysicsChinese Academy of SciencesJiading Campus Institute of Forensic Science Ministry of Public Security Radiology department Weifang People's Hospital

Deoxyribonucleic acid (DNA) damage arising from radiations widely occurred along with the development of nuclear weapons and clinically wide application of computed tomography (CT) scan and nuclear medicine. All ionizing radiations (X-rays, γ-rays, alpha particles, etc.) and ultraviolet (UV) radiation lead to the DNA damage. Polymerase chain reaction (PCR) is one of the most wildly used techniques for detecting DNA damage as the amplification stops at the site of the damage. Improvements to enhance the efficiency of PCR are always required and remain a great challenge. Here we establish a multiplex PCR assay system (MPAS) that is served as a robust and efficient method for direct detection of target DNA sequences in genomic DNA. The establishment of the system is performed by adding a combination of PCR enhancers to standard PCR buffer. The performance of MPAS was demonstrated by carrying out the direct PCR amplification on 1.2 mm human blood punch using commercially available primer sets which include multiple primer pairs. The optimized PCR system resulted in high quality genotyping results without any inhibitory effect indicated and led to a full-profile success rate of 98.13%. Our studies demonstrate that the MPAS provides an efficient and robust method for obtaining sensitive, reliable and reproducible PCR results from human blood samples.

关键词： DNA检测血液样本计算机断层扫描 FTA 多重PCR DNA损伤稳健脱氧核糖核酸

A highly sensitive chemiluminescence sensor for detecting mercury(Ⅱ) ions: a combination of Exonuclease Ⅲ-aided signal amplification and graphene oxide-assisted background reduction

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Science China Chemistry 2015年第3期58卷 514-518页

作者： Yang Tian Yue Wang Yan Xu Yang Liu Di Li chunhai fan College of Life and Environmental Science Minzu University of China Division of Physical Biology & Bioimaging Center Shanghai Synchrotron Radiation Facility Shanghai Institute of Applied PhysicsChinese Academy of Sciences Department of Chemistry Beijing Key Laboratory for Analytical Methods and Instrumentation Tsinghua University

In this paper, we report a highly sensitive chemiluminescence(CL) sensor for Hg2+ ions based on thymine-Hg2+-thymine(T-Hg2+-T) coordination chemistry. We designed a thymine rich oligonucleotide as a capture probe and a signal probe that includes two functional domains: a horseradish peroxidase-mimicking DNAzyme domain for the generation of CL, and a recognition domain. Graphene oxide(GO) was introduced to adsorb the signal probe via π-π interaction, which brought the DNAzyme domain and GO into close proximity and quenches CL. In the presence of Hg2+ ions, the coordination of Hg2+ with the capture probe yielded a hairpin complex, triggers cascaded strand displacement reactions and Exonuclease III-assisted signal amplifications. As a result, accumulated amounts of DNAzyme were generated and released from GO, leading to an enhanced CL signal. This strategy combines enzyme-based signal amplification and GO as a background reducer, leads to a limit of detection(LOD) of 2 nmol/L. This simple detection system provides a label-free yet sensitive approach for detection of Hg2+ ions.

关键词： graphene oxide chemiluminescence mercury(II) ions DNAzyme