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The in ovo injection of methionine improves intestinal cell proliferation and differentiation in chick embryos by activating the JAK2/STAT3 signaling pathway

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Animal Nutrition 2021年第4期7卷 1031-1038页

作者： Meng-Jie Chen Jia-Yi Zhou Yi-Jun Chen Xiu-qi Wang Hui-Chao Yan chun-qi gao College of Animal Science South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Nutrition ControlGuangzhou510642China

The intestinal health of chick embryos is vital for their life-long growth,and exogenous nutrition intervention may provide sufficient nutrition for embryonic *** the present study,we investigated the effect of in ovo injection of L-methionine(L-Met)on the intestinal structure and barrier function of chick *** were 4 groups of treatments:the control(CON)group injected with phosphate-buffered saline(PBS)and the other 3 groups injected with 5,10,and 20 mg L-Met/egg,*** injection was performed on embryonic day 9(E9),and intestinal samples were collected on the day of hatching for *** results showed that,compared with the CON group,the groups administered an in ovo injection of L-Met increased relative weights of the duodenum,jejunum,and ileum(P<0.05).Hematoxylin and eosin(H&E)staining showed that the groups injected with 5,10,and 20 mg L-Met significantly increased villus height and crypt depth(P<0.05).Moreover,in ovo injection of 10 mg L-Met also increased the transepithelial electrical resistance(TEER)of the jejunum(P<0.05).Injection with 10 and 20 mg L-Met increased the expression of the tight junction proteins(ZO-1 and claudin-1)and the fluorescence signal intensity of Ki67 and villin proteins(P<0.05).Further,the protein expression of phospho-Janus kinase 2(p-JAK2)and phospho-signal transducer and activator of transcription 3(p-STAT3)was significantly increased by 10 or 20 mg L-Met injection(P<0.05).In conclusion,the injection of L-Met,especially at a dose of 10 mg,showed beneficial effects on the intestinal integrity of chick embryos due to the activation of the JAK2/STAT3 signaling *** results may provide new insights for regulating the intestinal development of embryonic chicks and the rapid growth of chicks after hatching.

关键词： In ovo injection Chick Intestine L-methionine JAK2/STAT3

Dietary supplementation with acidifiers improves the growth performance, meat quality and intestinal health of broiler chickens

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Animal Nutrition 2021年第3期7卷 762-769页

作者： chun-qi gao Hui-qin Shi Wen-Yan Xie Li-Hong Zhao Jian-Yun Zhang Cheng Ji qiu-Gang Ma State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China College of Animal Science South China Agricultural UniversityGuangzhou510642China

This experiment was conducted to study the effects of dietary supplementation with acidifiers on the growth performance,meat quality,and intestinal health of broiler chickens.A total of 648 male Arbor Acres broiler chickens at 1 d old were randomly divided into 6 groups,and each group consisted of 6 replicates with 18 broilers per *** dietary treatments were as follows:negative control(NC,the basal diet),NC+antibiotic(enramycin,8 mg/kg,positive control[PC]),NC+phosphoric acid(PA,0.1,0.2,and 0.3 g/kg),and NC+lactic acid(LA,0.3 g/kg).The feeding trial lasted for 42 *** results showed that the feed-to-gain ratio of the NC+acidifier groups was lower than that of the NC and PC groups from 1 to 42 d(P<0.05).Compared with the values in the NC group,the pH of breast muscle was significantly higher in the NC+PA(0.2 g/kg)and LA(0.3 g/kg)groups(P<0.05),and the cooking loss was lower in the breast muscle of the NC+PA(0.1 g/kg)and LA(0.3 g/kg)groups(P<0.05).In addition,the shear force of the breast muscle and thigh muscle and the pH value in the crop,gizzard and duodenum of the antibiotic and acidifier groups were significantly decreased(P<0.05).Moreover,the trypsin,chymotrypsin,and lipase activities of the duodenum in the NC+PA(0.2 and 0.3 g/kg)groups,as well as the villus height-to-crypt depth(VH:CD)ratio of the duodenum in the NC+PA(0.1 g/kg)group was significantly greater(P<0.05)compared with those in the NC ***,the number of total aerobic bacteria,Escherichia coli and Salmonella in the cecum of the NC+PA(0.1 g/kg)and LA(0.3 g/kg)groups were decreased(P<0.05).Collectively,diet supplementation with acidifiers could improve the growth per-formance,meat quality,and intestinal health of broilers,in which the effects of PA(0.1 g/kg and 0.2 g/kg)are better than the other supplementations.

关键词： Antibiotic Acidifier Growth performance Meat quality Intestinal health Broiler

Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 549-559页

作者： chun-qi gao Yin-ling ZHAO Hai-chang LI Wei-guo SUI Hui-chao YAN Xiu-qi WANG College of Animal Science South China Agricultural University/National Engineering Research Center for Breeding Swine Industry /Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding Davis Heart & Lung Research Institute Wexner Medical Center at the Ohio State University

Proliferation suppression and apoptosis are the prominent characteristics induced by heat stress（HS） in cells, whereas the effects of HS on cell growth（mass accumulation） are unknown. In this study, Lantang swine（an indigenous breed of China） skeletal muscle satellite cells（SCs） were pre-cultured at 37 °C for 24 h. The HS group was subjected to HS at 41 °C, while the control group was maintained at 37 °C. Heat shock protein 70（HSP70） expression and SC size are significantly increased（P〈0.05） by HS, but cell proliferation is suppressed（P〈0.05） and apoptosis is induced（P〈0.05）. HS led to a lower percentage of SCs in the G0/G1 phase（P〈0.05） together with a higher percentage of SCs in the S phase（P〈0.05）. However, the percentage of SCs in the G2/M phase was decreased（P〈0.05） at 48 h but then increased（P〈0.05） at 72 h with HS. In addition, the phosphorylation ratios of protein kinase b（Akt）, ribosomal protein S6 kinase（S6K）, and ribosomal protein S6 were increased（P〈0.05） by HS. Nevertheless, the phosphorylation ratios of the 4E binding protein 1 and the eukaryotic initiation factor-4E were indistinguishable（P〉0.05） from those of the control group. The phosphorylation ratio of the mammalian target of rapamycin（m TOR）（Ser^2448） increased（P〈0.05） within 48 h, and apparent differences were abrogated at 72 h（P〉0.05）. Moreover, cleaved caspase-3 expression was increased at 72 h（P〈0.05）. These findings indicate that HS induces apoptosis and disrupts cell cycle distribution to decrease the number of cells. Additionally, HS can promote SC growth via an activated Akt/m TOR/S6 K signaling pathway.

关键词： Heat stress Swine Cell proliferation Cell growth Apoptosis Akt/mTOR/S6K pathway

Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon(Columba livia)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 511-523页

作者： Ming-xia CHEN Xiang-guang LI Jun-xian YANG chun-qi gao Bin WANG Xiu-qi WANG Hui-chao YAN College of Animal Science South China Agricultural University/Key Laboratory of Chicken GeneticsBreeding and ReproductionMinistry of Agriculture/Guangdong Provincial Key Laboratory of Agro-Animal Genomics/South China Collaborative Innovation Center for Poultry Disease Control and Product Safety Guangdong Fengli Agricultural Comprehensive Development Co. Ltd.

The objective of this study was to investigate the relationship between gene expression of nutrient（amino acid, peptide, sodium and proton） transporters in the small intestine and embryonic growth in domestic pigeons（Columba livia）. One hundred and twenty-five fertilized eggs were randomly assigned into five groups and were incubated under optimal conditions（temperature of 38.1 °C and relative humidity of 55%）. Twenty embryos/birds from each group were sacrificed by cervical dislocation on embryonic day（E） 9, 11, 13, 15 and day of hatch（DOH）. The eggs, embryos（without yolk sac）, and organs（head, brain, heart, liver, lungs, kidney, gizzard, small intestine, legs, and thorax） were dissected, cleaned, and weighed. Small intestine samples were collected for RNA isolation. The m RNA abundance of intestinal nutrient transporters was evaluated by real-time reverse transcription-polymerase chain reaction（RT-PCR）. We classified these ten organs into four types according to the changes in relative weight during embryonic development. In addition, the gene expression of nutrient transporters was differentially regulated by embryonic day. The m RNA abundances of b^0,＋AT, EAAT3, y^＋LAT2, Pep T1, LAT4, NHE2, and NHE3 increased linearly with age, whereas m RNA abundances of CAT1, CAT2, LAT1, EAAT2, SNAT1, and SNAT2 were increased to higher levels on E9 or E11 and then decreased to lower levels until DOH. The results of correlation analysis showed that the gene expressions of b^0,＋AT, EAAT3, Pep T1, LAT4, NHE2, NHE3, and y^＋LAT2 had positive correlations with body weight（0.71〈correlation coefficient（CC）〈0.82, P〈0.0001）, while CAT1, CAT2, EAAT2, SNAT1, and SNAT2 had negative correlations with body weight（-0.86〈CC〈-0.64, P〈0.0001）. The gene expressions of b^0,＋AT, EAAT3, LAT4, Pep T1, NHE2, NHE3, and y^＋LAT2 showed positive correlations with intestinal weight（0.80〈CC〈0.91, P〈0.0001）, while CAT1, CAT2, and EAAT2 showed negative correlations with intestinal weight（-0.84〈CC〈-0.67, P〈0.0

关键词： Embryonic growth Nutrient transporters Gene expression Small intestine Pigeons

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点云频域配准的双目双线结构光列车轮对检测

光学精密工程 2017年第3期25卷 616-624页

作者：王华邢春齐高金刚张爽朱可可长春工业大学机电工程学院吉林长春130012 长春工程学院机电工程学院吉林长春130012 长春理工大学机电工程学院吉林长春130022

针对单线结构光测量因范围有限以及遮挡等原因而无法检测整个车轮的轮缘及踏面的问题,提出了一种列车轮对双目双线结构光检测方法。该方法采用两组双线结构光传感器进行测量和数据采集。在两传感器产生的点云进行配准的过程中使用截面... 详细信息

针对单线结构光测量因范围有限以及遮挡等原因而无法检测整个车轮的轮缘及踏面的问题,提出了一种列车轮对双目双线结构光检测方法。该方法采用两组双线结构光传感器进行测量和数据采集。在两传感器产生的点云进行配准的过程中使用截面点云数据还原点云矩阵,然后对其进行傅里叶变换,在计算旋转角度时先进行极坐标转换,最后求取两矩阵的互功率谱,从而得到点云的旋转和平移矩阵。在此过程中考虑到点云噪声的存在,将辛格函数近似代替无噪声的互功率谱傅里叶反变换,从而确定在频域配准时噪声对配准参数的确定并无影响。接着采用相对值比较的算法和主成分分析拟合基准平面,对轮对直径方向进行标定。利用标定值对扫描数据进行处理,得出包括车轮直径、车轮滚动圆径向跳动等对尺寸参数。实验表明:点云频域配准的双目双线轮对检测技术对车轮直径的测量尺寸误差≤±0.05mm,车轮端跳的测量误差≤±0.06mm,车轮径跳的测量误差≤0.04mm。与标准轮对检测数据相比可知,该系统满足轮对检测精度的要求。

关键词：非接触测量结构光轮对检测双目双线点云频域配准

Meningeal lymphatic vessel crosstalk with central nervous system immune cells in aging and neurodegenerative diseases

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Neural Regeneration Research 2025年第3期20卷 763-778页

作者： Minghuang gao Xinyue Wang Shijie Su Weicheng Feng Yaona Lai Kongli Huang Dandan Cao qi Wang Science and Technology Innovation Center Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Institute of Clinical Pharmacology Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina The Sixth Affiliated Hospital Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain metabolites but also contribute to antigen delivery and immune cell activation. The advent of novel genomic technologies has enabled rapid progress in the characterization of myeloid and lymphoid cells and their interactions with meningeal lymphatic vessels within the central nervous system. In this review, we provide an overview of the multifaceted roles of meningeal lymphatic vessels within the context of the central nervous system immune network, highlighting recent discoveries on the immunological niche provided by meningeal lymphatic vessels. Furthermore, we delve into the mechanisms of crosstalk between meningeal lymphatic vessels and immune cells in the central nervous system under both homeostatic conditions and neurodegenerative diseases, discussing how these interactions shape the pathological outcomes. Regulation of meningeal lymphatic vessel function and structure can influence lymphatic drainage, cerebrospinal fluid-borne immune modulators, and immune cell populations in aging and neurodegenerative disorders, thereby playing a key role in shaping meningeal and brain parenchyma immunity.

关键词： central nervous system meningeal lymphatic vessels immunity myeloid cells lymphatic cells neurodegenerative disease

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long Xiao gao Zhengwei Wang Zhizhong Guan Xiaolan qi Wei Hong Yan Xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

基于世界模拟的政治非稳态:Sora的智能影像生成与历史重塑

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新疆师范大学学报（哲学社会科学版） 2025年第2期46卷 70-78页

作者：高奇琦台润泽华东政法大学政府管理学院上海201620 华东政法大学政治学研究院上海201620

OpenAI发布的文字转视频模型Sora在智能影像生成领域取得突破,被认为具备“世界模拟”能力。这种对现实物理世界的逼真模拟能力,引发人类对“真实性”的重新评估。通过模拟现实场景和历史事件,Sora在展示其应用潜力的同时,对真实性、历... 详细信息

OpenAI发布的文字转视频模型Sora在智能影像生成领域取得突破,被认为具备“世界模拟”能力。这种对现实物理世界的逼真模拟能力,引发人类对“真实性”的重新评估。通过模拟现实场景和历史事件,Sora在展示其应用潜力的同时,对真实性、历史真相和公众信任等带来挑战。文本介绍了Sora的技术背景,探讨了智能影像与虚假信息结合的风险。这一结合可能通过社会媒介影响公众信念和政治秩序,引发社会极化和分化。此外,在重塑历史记忆与认同时,可能导致信息的扭曲和伪造问题,或将社会推向政治非稳态。为应对技术带来的挑战,文本强调在迈向通用人工智能时代的过程中平衡技术创新与社会责任的重要性。通过深入分析Sora等智能影像生成的可能影响与挑战,提醒社会关注生成式人工智能技术的监管和伦理,确保技术发展的同时维护历史的真实性,保障社会福祉。

关键词： Sora 智能影像生成人工智能政治秩序

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Variational Calculation in SU(3) Lattice Gauge Theory

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Chinese Physics Letters 2001年第5期18卷 634-637页

作者： YANG chun ZHANG qi-Ren gao chun-Yuan Department of Technical Physics Peking UniversityBeijing 100871

Using the Hamiltonian lattice gauge theory,we perform some variational calculations to obtain the ground-state energy of SU(3)gauge field and scalar(0^(++))glueball *** agreement of our data with the strong and weak expansion results in the corresponding limits indicates that this method can provide us reliable information in the most interesting medium *** trial wave function used in our variational method is also proved to be a good first approximation of the ground-state of the SU(3)gauge *** this function according to correlations of adjacent plaquettes may mean better result.

关键词： field. SU(3) gauge