检索结果-南通市图书馆

同方期刊数据库

Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy

在线全文

学校读者我要写书评

暂无评论

Biomedical Engineering Frontiers 2023年第1期4卷 31-41页

作者： Anran Zheng Chao li Shengkai Xu Zhen Guo chuanyu li Changsong Zhang Jia Yao Zhiqi Zhang Jinze li Lutao Du Shasha Zhao Chuanxin Wang Wei Zhang lianqun Zhou CAS Key Lab of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou 215163China School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China Suzhou Hospital Affiliated Hospital of Medical SchoolNanjing UniversitySuzhou 215153Jiangsu ProvinceChina Suzhou CASENS Co.Ltd. Suzhou 215163China Ji Hua Laboratory Foshan 528000China Department of Clinical Laboratory The Second Hospital of Shandong UniversityJinan 250033ShandongChina

Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one *** work has substantial implications toward improving the efficiency of chronic CVD ***:Monitoring CVD of metabolites is strongly associated with disease *** and time-consuming detection in hospitals is unfavorable for chronic CVD ***:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one *** were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite ***:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 *** linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.

关键词： flex linking consuming

推动税收征管数字化转型增添服务中国式现代化新动能

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

税务研究 2023年第2期 26-30页

作者：李传玉国家税务总局内蒙古自治区税务局

税收在国家治理中发挥着基础性、支柱性、保障性作用,税收现代化是推进中国式现代化的重要条件和保障。当前,以税收现代化为统领、以智慧税务建设为目标、以“金税四期”为抓手的税收征管数字化转型正在蓬勃开展,成为深化税收征管改革... 详细信息

税收在国家治理中发挥着基础性、支柱性、保障性作用,税收现代化是推进中国式现代化的重要条件和保障。当前,以税收现代化为统领、以智慧税务建设为目标、以“金税四期”为抓手的税收征管数字化转型正在蓬勃开展,成为深化税收征管改革和税收现代化的重要支撑,也取得了阶段性成效。为加快推动税收现代化,增添服务中国式现代化新动能,税收征管数字化转型需要立足实际,进一步明确目标,找准问题,科学施策。本文全面梳理了当前我国税收征管数字化转型存在的问题,提出了推动我国税收征管数字化转型的创新性对策,为加快实现税收征管数字化转型提供了新思路。

关键词：税收现代化中国式现代化税收征管数字化转型智慧税务

DNA damage chemotherapeutic drugs suppress basal-like breast cancer growth by down-regulating the transcription of the FOXO1-KLF5 axis

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第1期11卷 91-94页

作者： Qiuxia Cui Jian Sun Jingping Yuan Juanjuan li chuanyu Yang Guangshi Du Chengang Zhou Pu Qiu Jidong Gao Yuanqi Zhang Dewei Jiang Ceshi Chen Affiliated Hospital of Guangdong Medical University Science&Technology of China ZhanjiangGuangdong 524000China Department of Breast Surgical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital and Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhenGuangdong 518116China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Department of Pathology Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Breast and Thyroid Surgery Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Translational Medicine Research Center of Guizhou Medical University GuiyangGuizhou 550025China Department of Breast Surgical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Yunnan Cancer Hospital and the Third Affiliated Hospital of Kunming Medical University and Yunnan Cancer Center KunmingYunnan 650500China Academy of Biomedical Engineering Kunming Medical UniversityKunmingYunnan 650500China

Most basal-like breast cancers(BLBCs)have a poor prognosis and high crossover with triple-negative breast cancers(TNBCs).However,approximately 50%of TNBC patients develop chemoresistance.^(1) Dexamethasone reportedly induces Krüppel-like factor 5(KLF5)and can cause docetaxel and cisplatin resistance in TNBC.A super-enhancer can maintain the transcription of KLF5.

关键词： FOXO1 drugs damage

Mobility,Constraint Singularity and Isotropy of the 3-R Translational Parallel Mechanism

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2009年第6期22卷 841-848页

作者： CHEN Qiaohong li Qinchuan WU chuanyu li Yi Provincial Key Laboratory of Modern Textile Machinery Zhejiang Sci-Tech University Hangzhou 310018 China

The non-overconstrained 3-degrees of freedom（DOF） translational parallel mechanism（TPM） has received much attention due to its advantages in reduced cost of fabrication and assembly. Researches are being conducted in the area of type synthesis, kinematic analysis and dimensional synthesis. Mobility, constraint singularity and isotropy of a 3-PRRRR non-overconstrained TPM are studied, where P denote the prismatic pair, R the revolute pair and the overline indicates the same axis direction of the kinematic pair The different arrangements of the three limbs affect the kinematic performance of this kind of TPM. First, the mobility analysis, actuation selection, and the constraint singularity of the general 3-PRRRR TPM are conducted based on screw theory. For a general 3-PRRRR TPM, the three prismatic pairs cannot be chosen as actuators and two kinds of constraint singularities are identified. In the first constraint singularity, the moving platform has four instantaneous DOFs. In the second constraint singularity, the moving platform has five instantaneous DOFs. Then, an orthogonal 3-PRRRR TPM is proposed, which can be actuated by three prismatic pairs and has no constraint singularities. Further, the forward and inverse kinematic analysis of the orthogonal TPM are presented. The input-output equations of the orthogonal TPM are totally decoupled. The full isotropy of the orthogonal TPM is proved by establishing the Jacobian matrix , which is an identity 3x3 diagonal matrix in the whole workspace. The orthogonal 3-PRRRR TPM has great potential in application like fast pick-and-place manipulator, parallel machine and micro-motion manipulator.

关键词： parallel manipulator kinematics screw theory

Integrated microdevice with a windmill-like hole array for the clog-free,efficient,and self-mixing enrichment of circulating tumor cells

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2022年第1期8卷 291-304页

作者： Hao li Jinze li Zhiqi Zhang Zhen Guo Changsong Zhang Zixu Wang Qiuquan Guo Chao li chuanyu li Jia Yao Anran Zheng Jingyi Xu Qingxue Gao Wei Zhang lianqun Zhou School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of China260026 HefeiChina CAS Key Lab of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of Sciences215163 SuzhouChina Ji Hua Laboratory 528000 FoshanChina Department of Laboratory Medicine The Affiliated Suzhou Science and Technology Town HospitalNanjing Medical University215153 SuzhouChina Shenzhen Institute for Advanced Study University of Electronic Science and Technology of China518000 ShenzhenChina Suzhou CASENS Co. Ltd215163 SuzhouChina Jinan Guoke Medical Technology Development Co. Ltd250001 JinanChina

Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive *** the limitations of affinity strategies based on their cost,effectiveness,and simplicity,size-based enrichment methods that involve low-cost,label-free,and relatively simple protocols have been further ***,the key challenges of these methods are clogging issues and cell aggregation,which reduce the recovery rates and *** by the natural phenomenon that the airflow around a windmill is disturbed,in this study,a windmill-like hole array on the SU-8 membrane was designed to perturb the fluid such that cells in a fluid would be able to self-mix and that the pressure acting on cells or the membrane would be dispersed to allow a greater *** addition,based on the advantages of fluid coatings,a lipid coating was used to modify the membrane surface to prevent cell aggregation and clogging of the *** the optimal conditions,recovery rates of 93%and 90%were found for A549 and HeLa cells in a clinical simulation test of our platform with a CTC concentration of 20-100 cells per milliliter of *** white blood cell(WBC)depletion rate was 98.7%(n=15),and the CTC detection limit was less than 10 cells per milliliter of blood(n=6).Moreover,compared with conventional membrane filtration,the advantages of the proposed device for the rapid(2 mL/min)and efficient enrichment of CTCs without clogging were shown both experimentally and *** to its advantages in the efficient,rapid,uniform,and clog-free enrichment of CTCs,our platform offers great potential for metastatic detection and therapy analyses.

关键词： logging free aggregation

电弧电流对AZ31B/DP980激光诱导电弧焊接接头成形及力学性能的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

材料工程 2022年第6期50卷 131-137页

作者：宋刚李传瑜郎强刘黎明大连理工大学材料科学与工程学院辽宁大连116024

采用激光诱导钨极惰性气体保护(tungsten inert gas,TIG)电弧焊接技术,在未添加任何夹层和镀层的条件下,通过优化工艺,获得了AZ31B镁合金和DP980高强钢高质量搭接焊接头,重点研究TIG电弧电流对焊接接头成形和力学性能的影响规律。结果表... 详细信息

采用激光诱导钨极惰性气体保护(tungsten inert gas,TIG)电弧焊接技术,在未添加任何夹层和镀层的条件下,通过优化工艺,获得了AZ31B镁合金和DP980高强钢高质量搭接焊接头,重点研究TIG电弧电流对焊接接头成形和力学性能的影响规律。结果表明:电弧电流的增大会提高镁合金在高强钢的润湿铺展能力,提升焊缝宽度的同时减小润湿角。镁合金/钢焊接接头的最大拉伸载荷随着电弧电流的增大先升高后降低,接头断裂模式由沿界面断裂转变为沿焊缝断裂。当TIG电流为80 A、激光功率为350 W时,焊接接头最大平均拉伸载荷达到279 N/mm。焊缝宽度和界面层厚度的增大以及激光匙孔的钉扎作用共同提升了镁合金/钢的接头性能。

关键词：激光诱导电弧镁合金高强钢接头成形力学性能

维普期刊数据库博看期刊

模具钢表面超声辅助激光熔覆Ni60合金涂层的仿真与实验分析

在线全文

学校读者我要写书评

暂无评论

制造技术与机床 2022年第7期 51-57页

作者：李传钰李金华姚芳萍辽宁工业大学机械工程与自动化学院辽宁锦州121001

采用超声辅助激光熔覆技术在H13热作模具钢表面制备镍基合金涂层,通过COMSOL仿真软件建立分析模型,探究超声频率对熔覆层温度及温度梯度的影响规律,并通过实验探究超声频率对宏观形貌以及显微组织的影响。结果表明:随着超声频率的增加,... 详细信息

采用超声辅助激光熔覆技术在H13热作模具钢表面制备镍基合金涂层,通过COMSOL仿真软件建立分析模型,探究超声频率对熔覆层温度及温度梯度的影响规律,并通过实验探究超声频率对宏观形貌以及显微组织的影响。结果表明:随着超声频率的增加,熔覆层温度逐渐增加,而温度差值呈先减小后增大的趋势;随着超声频率的增加,沿熔覆层切面纵向温度呈上升趋势,其温度差值呈先增大后减小的趋势;施加超声的熔覆层比未施加超声的温度梯度小,且超声频率越大,温度梯度越小;随着超声频率的增大,稀释率增大,熔覆层熔宽与熔深增大;超声频率越高,晶粒分离及细化程度越好;超声促进熔覆层与基体中元素扩散,改善元素偏析。

关键词：激光熔覆超声频率温度梯度稀释率晶粒细化元素偏析

在线全文

学校读者我要写书评

暂无评论

倏逝场照明的集成零模波导纳米孔芯片

光学精密工程 2022年第1期30卷 62-70页

作者：俞鹏飞付博文李传宇李超周连群郭振中国科学技术大学安徽合肥230026 中国科学院苏州生物医学工程技术研究所中国科学院生物医学检验技术重点实验室江苏苏州215163 复旦大学上海200433 苏州国科芯感医疗科技有限公司江苏苏州215163 季华实验室广东佛山528200

为了降低零模波导照明系统的成本、缩小尺寸,设计并完成衍射光栅、光波导以及零模波导的片上集成,并对集成化芯片的微纳结构及性能进行验证。采用时域有限差分法对集成化芯片进行了仿真设计,基于微纳加工手段制备出片上衍射光栅、光波... 详细信息

为了降低零模波导照明系统的成本、缩小尺寸,设计并完成衍射光栅、光波导以及零模波导的片上集成,并对集成化芯片的微纳结构及性能进行验证。采用时域有限差分法对集成化芯片进行了仿真设计,基于微纳加工手段制备出片上衍射光栅、光波导以及零模波导阵列结构,对微观结构进行表征,并借助荧光微球对芯片的性能进行验证。通过荧光微球测试,制备的集成化芯片可以实现荧光微球的有效激发;通过微观结构表征,衍射光栅周期为(352.8±2.6)nm,齿宽为(155.3±2.4)nm,刻蚀深度为(67.8±3.5)nm;光波导芯层的宽度为(504.05±10.35)nm,高度为(184.9±8.9)nm;零模波导直径为(200.2±6.4)nm,深度为(301.3±7.6)nm,满足设计要求。芯片尺寸为22 mm×22 mm,最小线宽为155 nm,通过8个衍射光栅、约1000条光波导以及数十万个零模波导阵列结构的片上集成,为零模波导的照明提供了一种紧凑且有效的解决方案。

关键词：集成光学光波导衍射光栅零模波导阵列

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

基于跨语种声学分析的帕金森病检测方法

电子与信息学报 2024年第2期46卷 546-554页

作者：季薇王传瑜吴迪李云郑慧芬南京邮电大学通信与信息工程学院南京210003 南京邮电大学计算机学院南京210023 南京医科大学附属老年医院南京210024

基于语音的帕金森病检测具有非介入式、成本较低和无创等优点。当前公开的帕金森病语音数据集大多来源于单一语种,存在数据容量不够大、受试者母语发音特点差异小等特点。单一语种数据集上训练的帕金森病检测模型在面对跨语种语音数据时... 详细信息

基于语音的帕金森病检测具有非介入式、成本较低和无创等优点。当前公开的帕金森病语音数据集大多来源于单一语种,存在数据容量不够大、受试者母语发音特点差异小等特点。单一语种数据集上训练的帕金森病检测模型在面对跨语种语音数据时,将出现性能下降。为避免语种差异带来的影响,提升模型在跨语种场景下的检测性能,该文引入对抗迁移学习和特征解耦的思想,提出一种帕金森病跨语种声学分析模型(CLSAM)。首先,将基于多头自注意力机制的Transformer编码块和多层神经网络级联,组成特征提取器模块,用于将从源域和目标域语音中提取的原始Fbank语音特征初步解耦为两个向量,即域不变病理信息表征向量和域信息表征向量;设计了目标任务不一致的双重对抗训练模块,显式地分离域不变病理信息和域信息;最终,提取跨语种语音数据中的域不变病理信息用于帕金森病检测。该文在公开的Maxlittle帕金森病语音数据集以及自采的帕金森病语音数据集上,采用十折交叉验证的方法验证了所提方法的有效性。实验结果表明:与传统机器学习方法以及现有的迁移学习算法相比,所提模型在跨语种场景中的检测准确率、敏感度和F1分数等性能均有明显提升。

关键词：跨语种声学分析帕金森病对抗迁移学习特征解耦

同方期刊数据库