检索结果-南通市图书馆

Engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2017年第5期15卷 279-286页

作者： Zhao Ma Xiaotian Wu christopher j.Krueger Antony K.Chen Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China Wallace H Coulter Department of Biomedical Engineering Georgia Institute of Technology Atlanta GA 30332 USA

With numerous advancements in novel biochemical techniques, our knowledge of the role of RNAs in the regulation of cellular physiology and pathology has grown significantly over the past several decades. Nevertheless, detailed information regarding RNA processing, trafficking, and localization in living cells has been lacking due to technical limitations in imaging single RNA transcripts in living cells with high spatial and temporal resolution. In this review, we discuss tech- niques that have shown great promise for single RNA imaging, followed by highlights in our recent work in the development of molecular beacons （MBs）, a class of nanoscale oligonucleotide-probes, for detecting individual RNA transcripts in living cells. With further refinement of MB design and development of more sophisticated fluorescence microscopy techniques, we envision that MB-based approaches could promote new discoveries of RNA functions and activities.

关键词： Y-O-methyl RNA Phosphorothioate Molecular beacon RNA dynamics Single-molecule RNAimaging

The Role of Chromatin Modifications in Progression through Mouse Meiotic Prophase

在线全文

学校读者我要写书评

暂无评论

j.urnal of Genetics and Genomics 2014年第3期41卷 97-106页

作者： j.mes H.Crichton christopher j.Playfoot Ian R.Adams MRC Human Genetics Unit MRC Institute of Genetics and Molecular MedicineUniversity of EdinburghWestern General Hospital

Meiosis is a key event in gametogenesis that generates new combinations of genetic information and is required to reduce the chro- mosome content of the gametes. Meiotic chromosomes undergo a number of specialised events during prophase to allow meiotic recombination, homologous chromosome synapsis and reductional chromosome segregation to occur. In mammalian cells, DNA phys- ically associates with histones to form chromatin, which can be modified by methylation, phosphorylation, ubiquitination and acetylation to help regulate higher order chromatin structure, gene expression, and chromosome organisation. Recent studies have identified some of the enzymes responsible for generating chromatin modifications in meiotic mammalian cells, and shown that these chromatin modifying enzymes are required for key meiosis-specific events that occur during meiotic prophase. This review will discuss the role of chromatin modifications in meiotic recombination, homologous chromosome synapsis and regulation of meiotic gene expression in mammals.

关键词： Mouse Meiosis Chromatin Chromosome Histone modification DNA methylation

Featuring the Nd:YAG laser capsulotomy in the operating room

在线全文

学校读者我要写书评

暂无评论

International j.urnal of Ophthalmology(English edition) 2020年第3期13卷 523-524页

作者： Veronique Promelle Sharon Armarnik christopher j.Lyons Department of Ophthalmology and Visual Sciences University of British ColumbiaVancouver V5Z 3N9Canada

Dear Editor,We read with interest the article of Kinori et al[1]titled’Pediatric Nd:YAG laser capsulotomy in the operating room:review of 87 cases’.Facilities for laser capsulotomy under general anesthesia are essen... 详细信息

关键词： Featuring the Nd:YAG laser capsulotomy in the operating room YAG

Short-course or long-course radiation therapy as a part of a neoadj.vant regimen for stage Ⅱ & Ⅲ rectal adenocarcinoma?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese j.urnal of Cancer Research 2019年第6期31卷 849-852页

作者： Nan Zhao christopher j.Lin Fei Wang Chi Lin Department of Radiation Oncology University of Nebraska Medical Center OmahaNE 68198USA University of Southern California Los Angeles CA 90007USA

The aim of this mini-review is to compare and contrast the pros and cons of short-course and long-course neoadj.vant chemoradiation therapy regimens for stageⅡ&Ⅲrectal *** trials have demonstrated the equal efficacy and safety of short-course and long-course radiation therapy as a part of neoadj.vant *** data also shows that total neoadj.vant therapy could be more successful than neoadj.vant chemoradiation followed by adj.vant *** review points out future research directions for patients with locally advanced rectal adenocarcinoma such as comparing total neoadj.vant therapy that contains a short-course of radiation therapy to the standard of care,and evaluating how the sequence of short-course radiation therapy and chemotherapy in the total neoadj.vant therapy impacts the pathological complete response(pCR)rate,local control,and survival outcomes.

关键词： Short-course long-course radiation therapy rectal cancer

Rhythms of chloroplast magnesium import contribute to daily metabolic feedback

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2022年第11期15卷 1659-1661页

作者： Xiang Li christopher R.Buckley Michael j.Haydon School of BioSciences University of MelbourneParkvilleVIC 3010Australia

Organisms have evolved stable yet dynamic circadian systems to coordinate biological rhythms that contribute to *** oscillators generate rhythms via transcriptional and post-transcriptional control of key cellular processes such as metabolism and nutrient transport that fluctuate across the course of the *** addition,intracellular ions and metabolites can act as signaling molecules and protein cofactors that provide feedback to fine-tune biological timekeeping(Haydon et al.,2013).

关键词： magnesium metabolism coordinate

Atypical group 1 neuraminidase pH1N1-N1 bound to a group 1 inhibitor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2015年第10期6卷 771-773页

作者： Ying Wu christopher j. Vavricka Yan Wu Qina Li Santosh Rudrawar Robin j.Thomson Mark yon Itzstein George F. Gao j.anxun Qi CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China Institute for Glycomics Griffith University Gold Coast Campus Nathan QLD 4222 Australia

Influenza virus is a member of the Orthomyxoviridae family and is the causative agent of seasonal and pandemic flu.

关键词： Influenza Influenza Virus Oseltamivir Plaque Reduction Assay I149V Mutant

Current trends in urethral stricture management

在线全文

学校读者我要写书评

暂无评论

Asian j.urnal of Urology 2014年第1期1卷 46-54页

作者： christopher j.Hillary Nadir I.Osman christopher R.Chapple Department of Urology Royal Hallamshire HospitalSheffieldUK

The recent International Consultation on Urological Disease(ICUD)panel 2010 confirmed that a urethral stricture is defined as a narrowing of the urethra consequent upon ischaemic spongiofibrosis,as distinct from sphincter stenoses and a urethral disruption *** possible,an anastomotic urethroplasty should be performed because of the higher success rate as compared to augmentation *** is some debate currently regarding the critical stricture length at which an anastomotic procedure can be used,but clearly the extent of the spongiofibrosis and individual anatomical factors(the length of the penis and urethra)are important,the limitation for this being extension of dissection beyond the peno-scrotal j.nction and the subsequent production of *** recently,there has been interest in whether to excise and anastomose or to carry out a stricturotomy and reanastomosis using a Heineke-Miculicz *** urethroplasty has evolved towards the more extensive use of oral mucosa grafts as compared to penile skin flaps,as both flaps and grafts have similar efficacy and certainly the use of either dorsal or ventral positioning seems to provide comparable *** is important that the reconstructive surgeon is well versed in the full range of available repair techniques,as no single method is suitable for all cases and will enable the management of any unexpected anatomical findings discovered intra-operatively.

关键词： Urethral stricture Stricture Lichen sclerosus Pelvic fracture Urethral inj.ry Bladder outflow obstruction Urethroplasty Buccal mucosa graft

Formal methods, statistical debugging and exploratory analysis in support of system development: Towards a verification and validation calculator tool

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International j.urnal of Modeling, Simulation, and Scientific Computing 2016年第1期7卷 120-141页

作者： Saikou Y.Diallo Ross Gore christopher j.Lynch j.se j.Padilla Virginia Modeling Analysis and Simulation Center Old Dominion University 1030 University Blvd.SuffolkVA 23435USA

In this paper,we propose an approach to formally verify and rigorously validate a simulation system against the specification of the real *** implement the approach in a verification and validation calculator tool that takes as input a set of statements that capture the requirements,internal conditions of the system and expected outputs of the real system and produces as output whether the simulation satisfies the requirements,faithfully represents the internal conditions of the system and produces the expected *** provide a use case to show how subj.ct matter experts can apply the tool.

关键词： Verification and validation formal methods modeling and simulation

Granular titanite from the Roter Kamm crater in Namibia:Product of regional metamorphism,not meteorite impact

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Geoscience Frontiers 2022年第3期13卷 156-163页

作者： Aaron j.Cavosie christopher j.Spencer Noreen Evans Kai Rankenburg Robert j.Thomas Paul H.Macey Space Science and Technology Centre and The Institute for Geoscience Research School of Earth and Planetary ScienceCurtin UniversityPerthWA 6102Australia Dept.of Geological Sciences and Geological Engineering Queen’s UniversityOntarioCanada j.hn de Laeter Centre Curtin UniversityPerthWA 6102Australia Council for Geoscience BellvilleWestern CapeSouth Africa

Accessory minerals with so-called granular texture have risen in importance as geochronological tools for U-Pb dating of meteorite impact events. Grain-scale recrystallization, typically triggered by a combination of high-strain deformation and post-impact heating, can create a polycrystalline microstructure consisting of neoblasts that expel radiogenic Pb, which are thus ideal for isotopic dating. While granular domains in zircon and monazite from shocked rocks have been demonstrated to preserve impact ages,few U-Pb dating studies have been conducted on granular microstructures in titanite(CaTiSiO;). Here we report the occurrence of granular-textured titanite from ~2020 Ma granite basement rock exposed in the rim of the 4–5 Ma Roter Kamm impact structure in Namibia. Orientation mapping reveals two microstructurally distinct titanite populations: one consisting of strained/deformed grains, and the other consisting of grains that comprise aggregates of strain-free neoblasts. In situ U-Pb geochronology on 37 grains shows that most grains from both titanite populations yield indistinguishable U-Pb dates of ca.1025 Ma, consistent with the observed microstructures forming during the Mesoproterozoic Namaqua Orogeny. Only four grains preserved older age domains, recording ca. 1875 Ma Paleoproterozoic metamorphism. Two significant observations emerge:(1) none of the analyzed titanite grains yield the 2020 Ma igneous crystallization age previously established from zircon in the same sample, and(2) no age-resetting was detected that could be attributed to the 4 to 5 Ma Roter Kamm impact *** the similarity of the neoblastic microstructure to minerals from other sites with an established impact provenance, the granular texture and near-complete Pb-loss in titanite from Roter Kamm granite instead records a Paleo-to Mesoproterozoic polymetamorphic history, rather than Miocene age shockrelated processes. These results highlight the critical importance of grain-scale cont

关键词： Namibia Roter Kamm Titanite EBSD Geochronology