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Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation

Protein & Cell 2024年第7期15卷 512-529页

作者： Kai-Hui Zhang Fei-Fei Zhang Zhi-Ling Zhang Ke-Fei Fang Wen-Xing Sun Na Kong Min Wu Hai-Ou Liu Yan Liu Zhi Li qing-qing Cai Yang Wang Quan-Wei Wei Peng-cheng Lin Yan Lin Wei Xu Cong-Jian Xu Yi-Yuan Yuan Shi-Min Zhao The Obstetrics&Gynecology Hospital of Fudan University State Key Laboratory of Genetic EngineeringFudan UniversityShanghai 200090China Shanghai Key Laboratory of Metabolic Remodeling and Children’s Hospital of Fudan UniversityShanghai 200032China School of Life Sciences and Institutes of Biomedical Sciences Fudan UniversityShanghai 200438China Department of Nutrition and Food Hygiene School of Public HealthNantong UniversityNantong 226019China Reproductive Medicine Center The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Shanghai Fifth People’s Hospital of Fudan University Fudan UniversityShanghai 200240China Department of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210014China Key Laboratory for Tibet Plateau Phytochemistry of qinghai Province College of PharmacyQinghai University for NationalitiesXining 810007China Pediatric Research Institute Children’s Hospital Affiliated to Shandong University(Jinan Children’s Hospital)Jinan 250022China

Polycystic ovary syndrome(PCOS)is the leading cause of anovulatory *** understanding of the ovulation drivers hinders PCOS ***,we report that follicle stimulating hormone(FSH)controls follicular fluid(FF)glutamine levels to determine *** ovulation starts from FF-exposing granulosa cell(GC)*** glutamine,which decreases in pre-ovulation porcine FF,elevates in PCOS patients ***-glutamine chow to elevate FF glutamine inhibits mouse GC apoptosis and induces hormonal,metabolic,and morphologic PCOS ***,follicle-development-driving FSH promotes GC glutamine synthesis to elevate FF glutamine,which maintain follicle wall integrity by inhibiting GC apoptosis through inactivating ASK1-JNK apoptotic *** and glutamine inhibit the rapture of cultured murine *** removal or ASK1-JNK pathway activation with metformin or AT-101 reversed PCOS traits in PCOS models that are induced with either glutamine or *** suggest that glutamine,FSH,and ASK1-JNK pathway are targetable to alleviate PCOS.

关键词： ovulation glutamine granulosa cells PCOS FSH

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Therapeutic potential of urine-derived stem cells in renal regeneration following acute kidney injury:A comparative analysis with mesenchymal stem cells

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World Journal of Stem Cells 2024年第5期16卷 525-537页

作者： Fang Li Bin Zhao Lei Zhang Guo-qing Chen Li Zhu Xiao-Ling Feng Meng-Jia Gong cheng-Chen Hu Yuan-Yuan Zhang Ming Li Yong-Qiang Liu Department of General Surgery Chongqing General HospitalChongqing 401147China Department of Pediatric Research Institute Children’s Hospital of Chongqing Medical UniversityChongqing 401147China

BACKGROUND Acute kidney injury(AKI)is a common clinical syndrome with high morbidity and mortality *** use of pluripotent stem cells holds great promise for the treatment of ***-derived stem cells(USCs)are a novel and versatile cell source in cell-based therapy and regenerative medicine that provide advantages of a noninvasive,simple,and low-cost approach and are induced with high multidifferentiation *** these cells could serve as a potential stem cell source for the treatment of AKI has not been *** Stem cell markers with multidifferentiation potential were isolated from human amniotic *** severe combined immune deficiency(SCID)mice models were induced by means of an intramuscular injection with *** isolated from human-voided urine were administered via tail *** functional changes in the kidney were assessed by the levels of blood urea nitrogen and serum *** histologic changes were evaluated by hematoxylin and eosin staining and transferase dUTP nick-end labeling ***,we compared the regenerative potential of USCs with bone marrow-derived mesenchymal stem cells(MSCs).RESULTS Treatment with USCs significantly alleviated histological destruction and functional *** renal function was rapidly restored after intravenous injection of 5×105 human USCs into SCID mice with glycerol-induced AKI compared with injection of *** from secretion assays conducted in vitro demonstrated that both stem cell varieties released a wide array of cytokines and growth *** suggests that a mixture of various mediators closely interacts with their biochemical *** types of stem cells showed enhanced tubular cell prolif-eration and decreased tubular cell apoptosis,although USC treatment was not more effective than MSC *** found that USC therapy significantly improved renal function and histological damage,inhibited inflammation and apoptosis processes in the kid

关键词： Urine-derived stem cells Regenerative medicine Acute kidney injury Renal function recovery Cell therapy

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Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer malignant behaviors by activation of target of rapamycin kinase/autophagy signaling

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World Journal of Gastroenterology 2024年第26期30卷 3229-3246页

作者： Juan Sun Jin-Xiu Zhang Meng-Shi Li Meng-Bin Qin Ruo-Xi cheng qing-Ru Wu Qiu-Ling Chen Dan Yang Cun Liao Shi-Quan Liu Jie-An Huang Department of Gastroenterology The Second Affiliated Hospital of Guangxi Medical UniversityNanning 530007Guangxi Zhuang Autonomous RegionChina Department of Colorectal&Anal Surgery The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Monopolar spindle-binding protein 3B(MOB3B)functions as a signal transducer and altered MOB3B expression is associated with the development of human *** To investigate the role of MOB3B in colorectal cancer(CRC).METHODS This study collected 102 CRC tissue samples for immunohistochemical detection of MOB3B expression for association with CRC *** overexpression and knockdown of MOB3B expression were induced in CRC cell lines,changes in cell viability,migration,invasion,and gene expression were *** cell autophagy was detected using transmission electron microscopy,while nude mouse xenograft experiments were performed to confirm the in-vitro *** MOB3B expression was reduced in CRC vs normal tissues and loss of MOB3B expression was associated with poor CRC *** of MOB3B protein in vitro attenuated the cell viability as well as the migration and invasion capacities of CRC cells,whereas knockdown of MOB3B expression had the opposite effects in CRC *** the molecular level,microtubule-associated protein light chain 3 II/I expression was elevated,whereas the expression of matrix metalloproteinase(MMP)2,MMP9,sequestosome 1,and phosphorylated mechanistic target of rapamycin kinase(mTOR)was downregulated in MOB3B-overexpressing RKO *** contrast,the opposite results were observed in tumor cells with MOB3B *** nude mouse data confirmed these in-vitro findings,i.e.,MOB3B expression suppressed CRC cell xenograft growth,whereas knockdown of MOB3B expression promoted the growth of CRC cell *** Loss of MOB3B expression promotes CRC development and malignant behaviors,suggesting a potential tumor suppressive role of MOB3B in CRC by inhibition of mTOR/autophagy signaling.

关键词： Colorectal cancer Monopolar spindle-binding protein 3B Mechanistic target of rapamycin kinase Autophagy Prognosis

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Targeting colorectal cancer with Herba Patriniae and Coix seed:Network pharmacology,molecular docking,and in vitro validation

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3539-3558页

作者： cheng-Lei Wang Bing-Wei Yang Xin-Yan Wang Xue Chen Wei-Dong Li Hao-Yu Zhai Ying Wu Mu-YaoCui Jia-He Wu qing-Hui Meng Nan Zhang Department of Oncology Guang’anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100053China The First Clinical Medical College Shaanxi University of Chinese MedicineXianyang 712046Shaanxi ProvinceChina Department of Scientific Research Management Guang’anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100053China School of Clinical Medicine qinghai University Xining 810000Qinghai ProvinceChina School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 100029China

BACKGROUND Herba Patriniae and Coix seed(HC)constitute a widely utilized drug combination in the clinical management of colorectal cancer(CRC)that is known for its diuretic,anti-inflammatory,and swelling-reducing *** its efficacy has been demonstrated in a clinical setting,the active compounds and their mechanisms of action in CRC treatment remain to be fully *** To identify the active,CRC-targeting components of HC and to elucidate the mechanisms of action *** Active HC components were identified and screened using *** for each component were ***-related targets were obtained from human gene *** targets between HC and CRC were identified.A“drug-ingredient-target”network was created to identify the core components and targets *** Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses were conducted to elucidate the key pathways *** docking between core targets and key components was *** vitro experiments validated core *** Nineteen active components of HC were identified,with acacetin as the primary active *** predictive analysis identified 454 targets of the active compounds in *** mapping with 2685 CRC-related targets yielded 171 intervention targets,including 30 core *** and KEGG analyses indicated that HC may influence the phosphoinositide 3-kinase(PI3K)/Akt signaling *** docking showed that acacetin exhibited an optimal interaction with AKT1,identifying PI3K,AKT,and P53 as key genes likely targeted by HC during CRC *** inhibited HT-29 cell proliferation and migration,as well as promoted apoptosis,in *** blotting analysis revealed increased p53 and cleaved caspase-3 expression and decreased levels of p-PI3K,p-Akt,and survivin,which likely contributed to CRC *** Acacetin,the principal active compound in the HC pair,inhibited the proliferati

关键词： Colorectal cancer Baijiangcao(Herba Patriniae) Yiyiren(Coix seed) Acacetin Proliferation Migration Apoptosis Network pharmacology

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Therapeutic Effectiveness of Leukocyte-and Platelet-rich Fibrin for Diabetic Foot Ulcers:A Retrospective Study

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Current Medical Science 2024年第3期44卷 568-577页

作者： Fen WANG Xiao-ling ZHANG Jing ZHANG Song GONG Jing TAO Hui XIANG Xiao-qing FU Xu-na BIAN Xue-feng YU An-hui XU cheng-la YI Shi-ying SHAO Department of Endocrinology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Branch of National Clinical Research Center for Metabolic Diseases Wuhan430030China Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Trauma Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Diabetic foot ulcer(DFU)is one of the most serious complications of ***-and platelet-rich fibrin(L-PRF)is a second-generation autologous platelet-rich *** study aims to investigate the clinical effects of L-PRF in patients with diabetes in real clinical *** Patients with DFU who received L-PRF treatment and standard of care(SOC)from 2018 to 2019 in Tongji Hospital were *** clinical information including patient characteristics,wound evaluation(area,severity,infection,blood supply),SOC of DFU,and images of ulcers was retrospectively extracted and analyzed.L-PRF treatment was performed every 7±2 days until the ulcer exhibited complete epithelialization or an overall percent volume reduction(PVR)greater than 80%.Therapeutic effectiveness,including overall PVR and the overall and weekly healing rates,was *** Totally,26 patients with DFU were enrolled,and they had an ulcer duration of 47.0(35.0,72.3)*** severity and infection of ulcers varied,as indicated by the Site,Ischemia,Neuropathy,Bacterial Infection,and Depth(SINBAD)scores of 2–6,Wagner grades of 1–4,and the Perfusion,Extent,Depth,Infection and Sensation(PEDIS)scores of 2–*** initial ulcer volume before L-PRF treatment was 4.94(1.50,13.83)cm3,and the final ulcer volume was 0.35(0.03,1.76)*** median number of L-PRF doses was 3(2,5).A total of 11 patients achieved complete epithelialization after the fifth week of treatment,and 19 patients achieved at least an 80%volume reduction after the seventh *** overall wound-healing rate was 1.47(0.63,3.29)cm3/week,and the healing rate was faster in the first 2 weeks than in the remaining *** treatment did not change the percentage of complete epithelialization or healing *** Adding L-PRF to SOC significantly improved wound healing in patients with DFU independent of the ankle brachial index,SINBAD score,or Wagner grade,indicating that this method is appropriate for D

关键词： diabetic foot ulcer autologous platelet-rich plasma leukocyte-and platelet-rich fibrin standard of care percentage volume reduction

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Two aquaporins,PIP1;1 and PIP2;1,mediate the uptake of neonicotinoid pesticides in plants

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Plant Communications 2024年第5期5卷 176-188页

作者： Qun Wan Yixin Li Jinjin cheng Ya Wang Jing Ge Tingli Liu Liya Ma Yong Li Jianan Liu Chunli Zhou Haocong Li Xing Sun Xiaolong Chen qing X.Li Xiangyang Yu Key Laboratory of Food Quality and Safety of Jiangsu Province State Key Laboratory Breeding BaseNanjing 210014China Institute of Food Safety and Nutrition Jiangsu Academy of Agricultural Sciences50 Zhongling StreetNanjing 210014China Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization Nanjing Xiaozhuang University3601 Hongjin AvenueNanjing 211171China Department of Molecular Biosciences and Bioengineering University of Hawaii at ManoaHonoluluHI 96822USA

Neonicotinoids(NEOs),a large class of organic compounds,are a type of commonly used pesticide for crop *** uptake and accumulation in plants are prerequisites for their intra-and intercellular move-ments,transformation,and *** the molecular mechanisms that underpin NEO uptake by plants is crucial for effective application,which remains ***,we demonstrate that NEOs enter plant cells primarily through the transmembrane symplastic pathway and accumulate mainly in the *** plasma membrane intrinsic proteins discovered in Brassica rapa,BraPIP1;1 and BraPIP2;1,were found to encode aquaporins(AQPs)that are highly permeable to NEOs in different plant species and facilitate NEO subcellular diffusion and *** conserved transport function was further demonstrated in Xenopus laevis oocyte and yeast ***1;1 and BraPIP2;1 gene knockouts and interaction as-says suggested that their proteins can form functional *** of the potential of mean force indicated a negative correlation between NEO uptake and the energy barrier of BraPIP1;1 *** study shows that AQPs transport organic compounds with greater osmolarity than previously thought,providing new insight into the molecular mechanisms of organic compound uptake and facilitating innovations in systemic pesticides.

关键词： neonicotinoid aquaporin Brassica rapa plasma membrane uptake systemic pesticide

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Miller fisher syndrome with positive anti-GQ1b/GT1a antibodies associated with COVID-19 infection:A case report

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World Journal of Clinical Cases 2024年第31期12卷 6500-6505页

作者： cheng-Qun Wei Xuan Yu Yuan-Yuan Wu qing-Jie Zhao Department of Neurology Huai’an HospitalHuai’an Cancer HospitalHuai’an 223001Jiangsu ProvinceChina Department of Neurology The First Affiliated Hospital of Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina

BACKGROUND Miller fisher syndrome(MFS)is a variant of Guillain-Barrésyndrome,an acute immune-mediated peripheral neuropathy that is often secondary to viral ***-ganglioside antibodies play crucial roles in the development of *** positive rate of ganglioside antibodies is exceptionally high in MFS patients,particularly for anti-GQ1b ***,the presence of other ganglioside antibodies does not exclude *** SUMMARY We present a 56-year-old female patient who suddenly developed right blepharoptosis and progressively worsening vision in both *** were flu symptoms prior to onset,and a coronavirus disease 2019 test was *** physical examination,the patient exhibited bilateral extraocular muscle paralysis,weakened reflexes in both limbs,and impaired *** cerebrospinal fluid examination results showed no obvious *** peroneal nerve F-waves were not *** anti-GD1b IgG and anti-GT1a IgG antibodies were *** patient received intravenous methylprednisolone(1000 mg/day),with the dosage gradually ***,intravenous high-dose immunoglobulin treatment was administered for 5 days(0.4 g/kg/day)from day 2 to day 6 of *** patient’s symptoms improved after treatment with immunoglobulins and *** Positive ganglioside antibodies may be used as supporting evidence for the diagnosis;however,the diagnosis of MFS is more reliant on clinical symptoms.

关键词： Miller fisher syndrome Guillain-Barre syndrome COVID-19 Anti-GQ1b antibody Anti-GT1a antibody Case report

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钢桥中错位厚板十字接头的力学性能与补强方法

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东北大学学报（自然科学版） 2024年第5期45卷 729-737页

作者：沈翀苏庆田陈友生同济大学土木工程学院上海200092 四川路桥华东建设有限责任公司四川成都610200

针对钢桥中厚板十字接头出现错位缺陷的问题,采用有限元方法研究了错位缺陷对厚板十字接头力学性能的影响,提出一种分离式焊接钢板补强方法并对补强钢板的尺寸进行优化设计.研究结果表明,错位缺陷对厚板十字接头的力学性能产生较大的影... 详细信息

针对钢桥中厚板十字接头出现错位缺陷的问题,采用有限元方法研究了错位缺陷对厚板十字接头力学性能的影响,提出一种分离式焊接钢板补强方法并对补强钢板的尺寸进行优化设计.研究结果表明,错位缺陷对厚板十字接头的力学性能产生较大的影响,板件竖向应力在附加弯矩作用下显著增大.提出的焊接钢板补强方法使得补强后竖向支承板承担大部分轴力与弯矩,强度控制设计截面位于补强钢板底部焊缝处,可通过增加补强钢板的高度提升补强效率,最高可达48%.本文推荐了不同错位量下补强钢板的合理尺寸,可为类似工程中错位厚板十字接头的补强方法提供参考.

关键词：钢桥十字接头错位缺陷补强方法有限元方法

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基于DPM模型的变径管冲蚀磨损分析与结构改进

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科学技术与工程 2024年第26期24卷 11238-11245页

作者：华剑闫冲冲董小庆长江大学机械工程学院荆州434023 长江大学机械结构强度与振动研究所荆州434023 中石化四机石油机械有限公司荆州434024

针对变径管冲蚀失效的问题,基于DPM模型、Realizeble k-ε模型和颗粒磨损方程对变径管进行数值模拟计算,分析流速、颗粒直径、质量流量、过渡倾角、改进后过渡圆角对变径管的冲蚀磨损规律和改进后变径管的性能。结果表明:过渡段与小径... 详细信息

针对变径管冲蚀失效的问题,基于DPM模型、Realizeble k-ε模型和颗粒磨损方程对变径管进行数值模拟计算,分析流速、颗粒直径、质量流量、过渡倾角、改进后过渡圆角对变径管的冲蚀磨损规律和改进后变径管的性能。结果表明:过渡段与小径管相贯处冲蚀磨损最为严重;随流速、质量流量的增大和颗粒直径的减小,最大冲蚀速率逐渐增大,其中受流速的影响最大。过渡倾角由5°增加至35°,最大冲蚀速率在9.32×10^(-5) kg/(m^(2)·s)上下波动;由35°增加至85°,最大冲蚀速率整体上升、后大幅度下降;80°~85°时,最大冲蚀速率较小且受各因素的影响很小,尤其等于85°时,其最大冲蚀速率降低了23.5倍。结构改进后过渡圆角7.5~15 mm变径管的最大冲蚀速率小于改进前变径管,且冲蚀分布比较均匀;随过渡倾角的增大,结构改进后过渡圆角2.5~15 mm变径管与改进前变径管相比,管内最大压力相差逐渐减小,且改进后管内最大压力及应力均低于改进前变径管,管内压力最大降低了2.25倍。该结果可为实际作业中变径管的选用及结构设计与优化提供参考。

关键词：变径管数值模拟冲蚀磨损结构改进

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巯基配位的金属有机框架在高效电催化析氧中的应用

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高分子材料科学与工程 2024年第6期40卷 106-114页

作者：郑炜琼刘习奎李爽程冲四川大学高分子科学与工程学院高分子材料工程国家重点实验室四川成都610065

金属有机框架(MOFs)材料已被探索作为析氧反应(OER)的有效电催化剂。然而,在大电流密度下,MOFs结构不稳定的问题严重阻碍了其大规模应用。文中采用硫醇官能化的2,5-二巯基对苯二甲酸(H4DMBD)与FeNi金属离子配合制备了一种具有巯基S配位... 详细信息

金属有机框架(MOFs)材料已被探索作为析氧反应(OER)的有效电催化剂。然而,在大电流密度下,MOFs结构不稳定的问题严重阻碍了其大规模应用。文中采用硫醇官能化的2,5-二巯基对苯二甲酸(H4DMBD)与FeNi金属离子配合制备了一种具有巯基S配位的双金属MOFs(FeNi-DMBDC)。得益于独特的Fe-S和Ni-S配位结构,FeNi-DSBDC在碱性OER催化中表现出高活性和良好的稳定性,只需要274 mV的过电位就可达到100 mA/cm2的工作电流密度。同时,负载在导电载体碳布(CC)上的FeNi-DSBDC可以实现安培级的工作电流密度,在50mA/cm2和100mA/cm2的电流密度下具有长期稳定性(>200 h)。这不仅拓宽了MOFs材料在高效稳定的OER催化剂中的应用潜力,也为分子设计和配体调控策略在催化剂设计中的作用提供了新见解。

关键词：金属有机框架析氧反应电催化剂配位调控

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