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World Journal of Clinical Cases 2022年第12期10卷 3923-3929页

作者： Fang-Chi hsiao Yaa-Jyuhn James Meir Lung-Kun Yeh hsin-Yuan Tan ching-hsi hsiao David Hui-Kang Ma Wei-Chi Wu Hung-Chi Chen Department of Ophthalmology Chang Gung Memorial HospitalTaoyuan 333423Taiwan Biomedical Sciences College of MedicineChang Gung UniversityTaoyuan 33305Taiwan Department of Medicine College of MedicineChang Gung UniversityTaoyuan 33305Taiwan Center for Tissue Engineering Chang Gung Memorial HospitalTaoyuan 333423Taiwan

BACKGROUND Streptococcus mitis(***)is an opportunistic pathogen that can lead to severe ocular *** previous reports,penetrating keratoplasty(PK)was usually adopted for the treatment of persistent corneal *** report describes an unusual case of nonhealing descemetocele caused by *** treated by antibiotics plus amniotic membrane transplantation(AMT).CASE SUMMARY A 63-year-old woman presented with a right persistent corneal ulcer that she had suffered from for the past 9 *** culture of a corneal scraping yielded *** right eye descemetocele decreased in diameter from 3 to 0.8 mm after the continuous administration of topical vancomycin and ceftriaxone for 2 *** to the slow healing,AMT was *** corneal erosion healed and gradually became *** visual acuity recovered from initially counting fingers to 100/200 at the last follow-up,67 mo after *** Antibiotics plus AMT may be an effective alternative treatment other than PK to promote epithelializationand to reduce inflammation in the corneas complicated by S. mitis keratitis.

关键词： Persistent corneal ulcer Amniotic membrane transplantation Streptococcus mitis Case report

Molecular mechanism of therapeutic approaches for human gastric cancer stem cells

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World Journal of Stem Cells 2022年第1期14卷 76-91页

作者： hsi-Lung hsieh Ming-Chin Yu Li-ching Cheng Ta-Sen Yeh Ming-Ming Tsai Department of Nursing Division of Basic Medical SciencesChang-Gung University of Science and TechnologyTaoyuan 333Taiwan Research Center for Chinese Herbal Medicine College of Human EcologyChang Gung University of Science and TechnologyTaoyuan 333Taiwan Department of Neurology Chang Gung Memorial HospitalTaoyuan 333Taiwan Department of General Surgery Chang Gung Memorial Hospital at LinkouTaoyuan 333Taiwan College of Medicine Chang Gung UniversityTaoyuan 333Taiwan Department of General Surgery New Taipei Municipal TuCheng HospitalNew Taipei 236Taiwan Department of General Surgery Chang Gung Memorial HospitalChiayi 613Taiwan

Gastric cancer(GC)is a primary cause of cancer-related mortality worldwide,and even after therapeutic gastrectomy,survival rates remain *** presence of gastric cancer stem cells(GCSCs)is thought to be the major reason for resistance to anticancer treatment(chemotherapy or radiotherapy),and for the development of tumor recurrence,epithelial–mesenchymal transition,and ***,GCSCs have the capacity for self-renewal,differentiation,and tumor *** also synthesize antiapoptotic factors,demonstrate higher performance of drug efflux pumps,and display cell plasticity ***,the tumor microenvironment(TME;tumor niche)that surrounds GCSCs contains secreted growth factors and supports angiogenesis and is thus responsible for the maintenance of the growing ***,the genesis of GCSCs is unclear and exploration of the source of GCSCs is *** this review,we provide up-todate information about GCSC-surface/intracellular markers and GCSC-mediated pathways and their role in tumor *** information will support improved diagnosis,novel therapeutic approaches,and better prognosis using GCSC-targeting agents as a potentially effective treatment choice following surgical resection or in combination with chemotherapy and *** date,most anti-GCSC blockers when used alone have been reported as unsatisfactory anticancer ***,when used in combination with adjuvant therapy,treatment can *** providing insights into the molecular mechanisms of GCSCs associated with tumors in GC,the aim is to optimize anti-GCSCs molecular approaches for GC therapy in combination with chemotherapy,radiotherapy,or other adjuvant treatment.

关键词： Gastric cancer stem cells Stem cell-surface markers Tumor niche Tumor microenvironment

The compounds fromDerris laxiflora Benth suppresses lipopolysaccharideinduced inflammatory response in murine Raw264.7 cells

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中国药理学与毒理学杂志 2015年第S1期29卷 64-65页

作者： Ai-jen YANG hsi-lin CHIU Ming-yu CHAO Jui-ching CHEN Department of Biotanicals Medical and Pharmaceutical Industry Technology and Development Center

OBJECTIVE The emerging role of chronic inflammation is the major degenerative diseases of modern society such as periodontitis,atherosclerosis,rheumatoid arthritis,Parkinson′s disease and even *** components were isolated from Derris laxiflora Benth.,In this study,we found these compounds from Derris laxiflora Benth suppress lipopolysaccharide-induced inflammatory response in murine macrophage(RAW 264.7)*** RAW 264.7cells were cultured in DMEM media supplemented with 10%(V/V)heated-inactivated FBS,penicillin 100U·mL-1 and streptomycin 100μg·*** cells were incubated at 37℃in a humidified atmosphere of 5%CO2in ***264.7cells were seeded in a 24-well plate at a density of 2×105 mL-1 and then incubated with or without LPS(100ng·mL-1)in the absence or presence of compounds for 24 *** of these isolates on NO production were measured indirectly by analysis of nitrite levels using the Griess *** was used as a positive *** ight components were isolated from Derris laxiflora Benth.,including three new pterocarpans 7,6′-dihydroxy-3′-methoxypterocarpan(1),derrispisatin(2),derriscoumaronochromone(3)and three new flavonoids cis-3,4′-dihydroxy-5,7-dimethoxyflavan(4),derriflavanone B(5),iso-lupinenol(6)as well as two known ones,lonchocarpol A(7)and lonchocarpol D(8).The structures of these new compounds were determined by analysis of their spectroscopic ***264.7 cells were treated with the compounds from Derris laxiflora Benth for 24 *** them,compounds 5,7 and 8 significantly suppressed the NO production in LPS-treated RAW264.7 cells with IC50 values<10μg·*** In this study,we found that compounds from Derris laxiflora Benth suppresses lipopolysaccharide-induced inflammatory response in murine Raw264.7 cells.

关键词： lipopolysaccharide RAW264.7cells Derris laxiflora

Quercetin exerts anti-inflammatory effects via inhibiting tumor necrosis factor-α-induced matrix metalloproteinase-9 expression in normal human gastric epithelial cells

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World Journal of Gastroenterology 2022年第11期28卷 1139-1158页

作者： hsi-Lung hsieh Ming-Chin Yu Li-ching Cheng Mei-Yi Chu Tzu-Hao Huang Ta-Sen Yeh Ming-Ming Tsai Department of Nursing Division of Basic Medical SciencesChang-Gung University of Science and TechnologyTaoyuan 333TaiwanChina Research Center for Chinese Herbal Medicine College of Human EcologyChang Gung University of Science and TechnologyTaoyuan 333TaiwanChina Department of Neurology Chang Gung Memorial HospitalTaoyuan 333TaiwanChina Department of General Surgery New Taipei Municipal TuCheng HospitalNew Taipei 236TaiwanChina College of Medicine Chang Gung UniversityTaoyuan 333TaiwanChina Department of General Surgery Chang Gung Memorial HospitalTaoyuan 333TaiwanChina

BACKGROUND Gastric injury is the most common digestive system disease worldwide and involves inflammation,which can lead to gastric ulcer or gastric cancer(GC).Matrix metallopeptidase-9[MMP-9(gelatinase-B)]plays an important role in inflammation and GC *** and quercetin-rich diets represent potential food supplements and a source of medications for treating gastric injury given their anti-inflammatory ***,the effects and mechanisms of action of quercetin on human chronic gastritis and whether quercetin can relieve symptoms remain *** To assess whether tumor necrosis factor-α(TNF-α)-induced MMP-9 expression mediates the anti-inflammatory effects of quercetin in normal human gastric mucosal epithelial *** The normal human gastric mucosa epithelial cell line GES-1 was used to establish a normal human gastric epithelial cell model of TNF-α-induced MMP-9 protein overexpression to evaluate the antiinflammatory effects of *** cell counting Kit-8 assay was used to evaluate the effects of varying quercetin doses on cell viability in the normal GES-1 cell *** migration was measured using Transwell *** expression of proto-oncogene tyrosine-protein kinase Src(cSrc),phospho(p)-c-Src,extracellular-signal-regulated kinase 2(ERK2),p-ERK1/2,c-Fos,p-c-Fos,nuclear factor kappa B(NF-κB/p65),and p-p65 and the effects of their inhibitors were examined using Western blot analysis and measurement of luciferase activity.p65 expression was detected by ***-9 m RNA and protein levels were measured by quantitative reverse transcription polymerase chain reaction(q RT–PCR)and gelatin zymography,*** q RT-PCR and gelatin zymography showed that TNF-αinduced MMP-9 m RNA and protein expression in a dose-and time-dependent *** effects were reduced by the pretreatment of GES-1 cells with quercetin or a TNF-αantagonist(TNFR inhibitor)in a dose-and timedependent *** and TNF-αa

关键词： Anti-inflammatory Quercetin Matrix metallopeptidase-9 Tumor necrosis factor-α Normal human gastric epithelial cells

Determination of Impurities and Degradation Products/Causes for <i>m</i>-Iodobenzylguanidine Using HPLC-Tandem Mass Spectrometry

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American Journal of Analytical Chemistry 2019年第11期10卷 590-607页

作者： Wei-hsi Chen Yu Chang ching-Yun Lee Shiu-Wen Liu Wen-ching Wu Tsai-Yueh Luo Chemistry Division Institute of Nuclear Energy Research Taiwan Technology Promotion Center Institute of Nuclear Energy Research Taiwan

m-Iodobenzylguanidinium hemisulfate (MIBGHS) is a precursor in the preparation of radioiodine-labeled m-iodobenzylguanidine (MIBG), which is used as a radio-imaging and therapy agent for neuroendocrine tumors and myocardial sympathetic nerve function. To ensure the quality and efficacy of the medicine and prevent side effects, the precursor purity, source of impurity, and derivatives have to be determined. In this study, the purity of synthesized MIBGHS and the amount of contaminants therein were determined by high-performance liquid chromatography and ultraviolet detection, gradient eluted by ammonium formate aqueous solution and acetonitrile mobile phase on both C8 and phenyl type column. The impurities were identified on the basis of molecular and fragmented ion mass spectra using of electrospray ionization triple quadrupole tandem mass spectrometry. The results revealed the presence of process-related impurities including m-bromobenzylguanidine (MBrBG) and overreacted byproducts. Stress test results indicated that MIBGHS is stable under acidic and dry thermal conditions for at least 72 h but MIBG aqueous solution was deteriorated slowly when exposed to UV light, thermal, oxidative and alkaline environments. Thus, m-iodobenzylamine, the starting material intended for the synthesis of MIBGHS should be analyzed to ensure that it is free from m-bromobenzylamine impurity. Stored under normal condition (-18°C), MIBGHS is stable for at least 12 months. The chemically labile guanidine and amine groups in MIBGHS are the major causes of its instability, while iodide loss from the phenyl group is a minor cause.

关键词： MIBG m-Iodobenzylguanidine Neuroendocrine Tumor Degradation Derivative Impurity Identification Tandem Mass Spectrometry

Cost-effectiveness analysis of population-based screening of hepatocellular carcinoma: Comparing ultrasonography with two-stage screening

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World Journal of Gastroenterology 2016年第12期22卷 3460-3470页

作者： Ming-Jeng Kuo hsiu-hsi Chen Chi-Ling Chen Jean ching-Yuan Fann Sam Li-Sheng Chen Sherry Yueh-hsia Chiu Yu-Min Lin Chao-Sheng Liao Hung-Chuen Chang Yueh-Shih Lin Amy Ming-Fang Yen Department of Hepatogastroenterology Tainan Municipal Hospital Tainan 701 Taiwan Graduate Institute of Epidemiology and Preventive Medicine College of Public Health National Taiwan University Taipei 100 Taiwan Graduate Institute of Clinical Medicine College of Medicine and Graduate Institute of Epidemiology and Preventive Medicine College of Public Health National Taiwan University Taipei 100 Taiwan Department of Health Industry Management School of Healthcare Management Kainan University Taoyuan 338 Taiwan School of Oral Hygiene College of Oral Medicine Taipei Medical University Taipei 110 Taiwan Department and Graduate Institute of Health Care Management Chang Gung University Taoyuan 333 Taiwan Division of Gastroenterology Department of Internal Medicine Shin-Kong Wu Ho-Su Memorial Hospital Taipei 111 Taiwan angder Health Clinics Taipei 111 Taiwan

AIM: To assess the cost-effectiveness of two populationbased hepatocellular carcinoma(HCC) screening programs, two-stage biomarker-ultrasound method and mass screening using abdominal ultrasonography(AUS).METHODS: In this study, we applied a Markov decision model with a societal perspective and a lifetime horizon for the general population-based cohorts in an area with high HCC incidence, such as Taiwan. The accuracy of biomarkers and ultrasonography was estimated from published meta-analyses. The costs of surveillance, diagnosis, and treatment were based on a combination of published literature, Medicare payments, and medical expenditure at the National Taiwan University Hospital. The main outcome measure was cost per lifeyear gained with a 3% annual discount rate. RESULTS: The results show that the mass screening using AUS was associated with an incremental costeffectiveness ratio of USD39825 per life-year gained, whereas two-stage screening was associated with an incremental cost-effectiveness ratio of USD49733 per life-year gained, as compared with no screening. Screening programs with an initial screening age of 50 years old and biennial screening interval were the most cost-effective. These findings were sensitive to the costs of screening tools and the specificity of biomarker ***: Mass screening using AUS is more cost effective than two-stage biomarker-ultrasound screening. The most optimal strategy is an initial screening age at 50 years old with a 2-year inter-screening interval.

关键词： Two-stage biomarker-ultrasound screening One-stage abdominal ultrasonography screening Markov model Cost-effectiveness Sensitivity analysis Age

Pediatric gastric cancer presenting with massive ascites

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World Journal of Gastroenterology 2015年第11期21卷 3409-3413页

作者： Chien-Heng Lin Wei-ching Lin I-hsiu Lai Shu-Fen Wu Kang-hsi Wu An-Chyi Chen Department of Pediatrics Children’s HospitalChina Medical University Hospital College of Medicine School of MedicineChina Medical University Department of Radiology China Medical University Hospital College of Chinese Medicine School of Chinese MedicineChina Medical University

Gastric adenocarcinoma is quite rare in children and as a result very little experience has been reported on with regards to clinical presentation,treatment and *** describe the case of a 16-year-old boy presenting with abdominal fullness and poor appetite for 7 *** showed massive ascites and computed tomography imaging revealed the presence of gastric mucosa thickness with omentum *** diagnosis of gastric adenocarcinoma was biopsyproven *** gastric adenocarcinoma being quite rare in the pediatric patient population,we should not overlook the possibility of gastric adenocarcinoma when a child presents with distended abdomen and massive ascites.

关键词： Gastric adenocarcinoma Ascites Children

Cell Surface Transmembrane Protein Database for Detecting Potential Targets of Antibody Drug in Application of Cancer Therapies

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Journal of Life Sciences 2010年第7期4卷 46-52页

作者： Po-Yuan Chen Cheng-Shang Kao Mien-De Jhuo Tzu-Hurng Cheng Wei-Tse Hsu Che-Yen Ou Tzu-ching Shih Chieh-hsi Wu Department of Biological Science and Technology China Medical University Taichung 404 Taiwan Parexel-Apex International Taipei Taiwan Department of Medical Radiology Technology China Medical University and Department of Radiology China Medical UniversityHospital Taichung 404 Taiwan Department of Pharmacy China Medical University Taichung 404 Taiwan

Background： Antibody drug conjugated （ADC） is one kind of very important method of therapy to cancer diseases. In this research, the authors introduce BLAST and some other important algorithms to create transmembrane protein databases. These databases are acquired from well-known databases such as NCBI or Swiss-Prot as template, and then collect all possible transmembrane protein by using BLAST or physical character. After collect these databases, the authors will aim at each nucleotide sequences to design the probes of oligonucleotide microarray, which can detect the high express transmembrane proteins very efficiently. Finally, the authors can accelerate the anti-cancer drug discovery by using these databases. Result： This study constructed a web service, the Transmembrane Protein Database, to researchers that are interested in or need to oligonucleotide microarray probe design for detecting potential targets of antibody drug. With user friendly web based windows containing each necessary selections, users can easily choose the parameters and get the suitable probe design suggestions. Conclusion： Transmembrane protein database is very important and powerful in detecting cancers or other human disease. By using this database, the authors offer a good strategy in transmembrane protein research as well.

关键词： Antibody drug transmembrane protein databases.

Chronic Obstructive Pulmonary Disease in Stage Ⅰ Non-small Cell Lung Cancer That Underwent Anatomic Resection:The Role of a Recurrence Promoter

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Chronic Obstructive Pulmonary Disease in Stage Ⅰ Non-small ...

THE REACTION OF BIS[l,3-DIPHENYL-2-IMIDAZOLIDINYLIDENE] WITH SEVERAL PERHALO CARBOXYLIC ACIDS AND PERFLUOROPROPENE

第九届中国肿瘤学术大会暨第十五届海峡两岸肿瘤学术大会

作者： Chih-hsi Kuo ching-Yang Wu Kang-Yun Lee Shu-Min Lin Fu-Tsai Chung Yu-Lun Lo Chien-Ying Liu Te-Chih hsiung Cheng-Ta Yang Yi-Cheng Wu

Background:Despite the use of anatomic resection,the post-surgical recurrencerate remains high in early-stage non-small cell lung cancer（NSCLC）.Chronicinfl ammation plays a role in the mechanism that promotes tumor *** aimed to investigate the association between recurrence outcome and chronicinfl ammation-related co-morbidities in early-stage resected ***:Areview of medical records for recurrence outcome and co-morbidities,in termsof chronic obstructive pulmonary disease（COPD）,DM,asthma and cardiovascular diseases,was performed with 181 patients with stageⅠNSCLC that underwent anatomic ***:Subjects with T descriptors as T2a disease（49.5 vs.28.0%,p＜0.05）and the presence of COPD（42.4 vs.20.7%,p＜0.01）had a higher risk of tumor *** analysis for recurrence-free survival showed T descriptor as T2a（21.5 months ***,p＜0.05）and the presence of COPD（20.5months ***,p＜0.01）as signifi cant factors predicting reduced *** presence of COPD（HR:1.98;95%CI,1.29-.02,p＜0.01）and T descriptor as T2a（HR:2.01;95%CI,1.04-3.91,p＜0.05）remain independent predictors of reduced recurrence-free survival in the Cox regression *** with COPD were at higher risk of brain recurrence（OR:7.88;95%CI,1.50-41.3,p＜0.01）.In contrast,patients without COPD showed a tendency toward recurrence in bone and liver（OR:4.13;95%CI,1.08-15.8,p=0.05）.Conclusion:Subjects with COPD and T2a disease had a higher risk of *** role of COPD as a recurrence promotermerits further prospective investigation.

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Chinese Science Bulletin 1966年第7期24卷 302-304页

作者：马振中陈庆云蒋锡夔马明扬 Institute of Organic Chemistry Academia Sinica

For the reaction of Wanzlick’s dimer （I） with compounds containing 'active hydrogen' H—Y, we have proposed the following mechanism （see also [3,4b]）: In order to demonstrate the generality of this mechanism, the reactions of the perhalo carboxylic acids CCl3COOH, CF3COOH, n-C3F7COOH, and dimer （I） were studied. On the other hand, if Wanzlick’s claim that the dimer （I） could spontaneously dissociate into the nucleophilic carbenes （II） is true, then the latter should easily react with the electrophilic perfluoro-olefins. Therefore, the reaction of perfluoropropene with the dimer （I） was also investigated. Trichloroacetic acid was found to

关键词： perfluoro dimer carboxylic react spontaneously latter claim oligomer yielding diphenyl