The compounds fromDerris laxiflora Benth suppresses lipopolysaccharideinduced inflammatory response in murine Raw264.7 cells

作     者：Ai-jen YANG Hsi-lin CHIU Ming-yu CHAO Jui-ching CHEN 

作者机构：Department of BiotanicalsMedical and Pharmaceutical Industry Technology and Development Center 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2015年第29卷第S1期

页      面：64-65页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The project supported by Department of Industrial Technology,Ministry of Economic Affairs,Chinese Taipei Medical and Pharmaceutical Industry Technology and Development Center 

主　　题：lipopolysaccharide RAW264.7cells Derris laxiflora 

摘      要：OBJECTIVE The emerging role of chronic inflammation is the major degenerative diseases of modern society such as periodontitis,atherosclerosis,rheumatoid arthritis,Parkinson′s disease and even *** components were isolated from Derris laxiflora Benth.,In this study,we found these compounds from Derris laxiflora Benth suppress lipopolysaccharide-induced inflammatory response in murine macrophage(RAW 264.7)*** RAW 264.7cells were cultured in DMEM media supplemented with 10%(V/V)heated-inactivated FBS,penicillin 100U·mL-1 and streptomycin 100μg·*** cells were incubated at 37℃in a humidified atmosphere of 5%CO2in ***264.7cells were seeded in a 24-well plate at a density of 2×105 mL-1 and then incubated with or without LPS(100ng·mL-1)in the absence or presence of compounds for 24 *** of these isolates on NO production were measured indirectly by analysis of nitrite levels using the Griess *** was used as a positive *** ight components were isolated from Derris laxiflora Benth.,including three new pterocarpans 7,6′-dihydroxy-3′-methoxypterocarpan(1),derrispisatin(2),derriscoumaronochromone(3)and three new flavonoids cis-3,4′-dihydroxy-5,7-dimethoxyflavan(4),derriflavanone B(5),iso-lupinenol(6)as well as two known ones,lonchocarpol A(7)and lonchocarpol D(8).The structures of these new compounds were determined by analysis of their spectroscopic ***264.7 cells were treated with the compounds from Derris laxiflora Benth for 24 *** them,compounds 5,7 and 8 significantly suppressed the NO production in LPS-treated RAW264.7 cells with IC50 values10μg·*** In this study,we found that compounds from Derris laxiflora Benth suppresses lipopolysaccharide-induced inflammatory response in murine Raw264.7 cells.