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第九届全国儿科感染性疾病暨第十届全国儿童肝脏疾病学术会议纪要

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中华儿科杂志 2012年第4期50卷 315-317页

作者：无方峰舒赛男王建设中华医学会儿科学分会感染学组中华医学会感染病学分会儿童肝病和感染学组《中华儿科杂志》编辑委员会华中科技大学同济医学院附属同济医院儿科学系武汉430030 不详

第九届全国儿科感染性疾病暨第十届全国儿童肝脏疾病学术会议于2011年4月10至13日在江苏省扬州市召开。本次会议由中华医学会儿科学分会感染学组、中华医学会感染病学分会儿童肝病和感染学组和《中华儿科杂志》编辑委员会联合主办。来... 详细信息

第九届全国儿科感染性疾病暨第十届全国儿童肝脏疾病学术会议于2011年4月10至13日在江苏省扬州市召开。本次会议由中华医学会儿科学分会感染学组、中华医学会感染病学分会儿童肝病和感染学组和《中华儿科杂志》编辑委员会联合主办。来自全国20余省、市、自治区代表共150余人参加了会议，共收到稿件122篇，有10位专家进行专题讲座，31位代表大会发言，并对《儿童EB病毒相关性疾病的诊断指南与治疗原则》进行了热烈的讨论，会议还邀请加拿大专家主持“儿科感染性疾病未来10年的挑战”专题讨论会，现将会议的主要内容介绍如下。

关键词：儿科感染性疾病肝脏疾病中华医学会感染病学分会会议儿童学术 EB病毒相关性疾病《中华儿科杂志》

Construction of infectious cDNA clones of PRRSV:Separation of coding regions for nonstructural and structural proteins

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Science China(Life Sciences) 2008年第3期51卷 271-279页

作者： YUAN ShiShan & WEI ZuZhang Department of Animal infectious diseases, Key Laboratory of Animal Parasitology chinese Ministry of Agriculture, Shanghai Vet- erinary Research Institute, chinese Academy of Agricultural Sciences, Shanghai 200232, China Department of Animal infectious diseases Key Laboratory of Animal Parasitology Chinese Ministry of Agriculture Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the causative agent of the ongoing "porcine high fever syndrome" in China, is capable of genetic and antigenic mutations at high fre- quency. How to design vaccine rationally to keep up with the ever-changing prevalent PRRSV variant is of great interest. We developed an infectious cDNA clone of an attenuated strain of Type II PRRSV, and further manipulated the infectious cDNA clone by inserting polylinker between ORF1 and ORF2, en- coding for nonstructural- or structural-protein, respectively. The cDNA was generated from the cell-attenuated virus strain, APRRS, via RT-PCR, and followed by nucleotide sequencing and molecular cloning. The full-length of the APRRS genomic RNA was determined as 15521 nucleotides in length excluding poly(A) tail, which has a 99.7% nucleotide identity with that of PRRSV Nsp strain, also a vac- cine strain. Based on the nucleotide sequencing results, the full-length cDNA clone was assembled in pBlueScript vector, under the control of T7 promoter at the immediate 5′ terminus of genome. To dis- cern the rescued viruses from that of parental virus, a Mlu I restriction site was engineered into ORF5 coding region. Upon transfection of the in vitro transcripts of both the original and Mlu I-tagged cDNAs into MA-104 cells, typical PRRSV cytopathic effects were observed. The rescued viruses from the full-length cDNA clones displayed the same virological and molecular properties. Subsequently, PCR-based mutagenesis was conducted to separate the coding regions between PRRSV nonstructural genes, ORF1, and structural proteins, ORF2-ORF7. The synthetic RNA of such mutant clone, pCSA, was infectious and the rescued virus shared similar properties with that of the parental virus. This study provided a valuable tool for development of chimeric PRRSV as vaccine candidate offering cross-protection to various genetically diversified PRRSV strains, and a platform for further develop- ment of PRRSV a

关键词： PRRSV reverse genetic system coding region nonstructural protein

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乙型肝炎母婴阻断临床管理流程

中华肝脏病杂志 2017年第4期25卷 254-256页

作者：无侯金林中国肝炎防治基金会中华医学会感染病学分会中华医学会肝病学分会南方医科大学南方医院广州510515

母婴传播是乙型肝炎病毒（HBV）的重要传播途径，加强慢性HBV感染孕妇及其所分娩婴儿的规范化管理是切断母婴传播的有效途径，为规范我国HBV母婴阻断临床管理，我们组织感染病学、肝病学、免疫学与产科学专家参照国内外公认乙型肝炎防... 详细信息

母婴传播是乙型肝炎病毒（HBV）的重要传播途径，加强慢性HBV感染孕妇及其所分娩婴儿的规范化管理是切断母婴传播的有效途径，为规范我国HBV母婴阻断临床管理，我们组织感染病学、肝病学、免疫学与产科学专家参照国内外公认乙型肝炎防治指南和最新研究成果盱”形成专家共识。

关键词：肝炎乙型母婴传播临床管理

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肝细胞癌诊断的影像组学模型推荐专家共识

中华肝脏病杂志 2024年第7期32卷 631-636页

作者：中国研究型医院学会感染与炎症放射学专业委员会中华医学会放射学分会传染病学组中国科技产业化促进会数字健康专业委员会中国医院协会传染病分会传染病影像学组北京影像诊疗技术创新联盟李宏军陈天武郭大静周海鹰曹金明欧静不详首都医科大学附属北京佑安医院放射科北京100069 重庆医科大学附属第二医院放射科重庆400010 川北医学院附属医院放射科川北医学院第二临床医学院南充市中心医院放射科

原发性肝癌为国内外常见的恶性肿瘤,2020年成为全球范围内第7大威胁人类健康的恶性肿瘤,且病死率高居癌症第4位。精准诊断对原发性肝癌的预后至关重要。影像组学作为新兴无创的数据分析方法,从影像图像中提取大量高通量特征,为临床诊疗... 详细信息

原发性肝癌为国内外常见的恶性肿瘤,2020年成为全球范围内第7大威胁人类健康的恶性肿瘤,且病死率高居癌症第4位。精准诊断对原发性肝癌的预后至关重要。影像组学作为新兴无创的数据分析方法,从影像图像中提取大量高通量特征,为临床诊疗决策制定提供更加准确的依据。目前,针对肝细胞癌诊断的影像组学模型多种多样,但缺乏共识。该共识基于当前最新研究证据,结合循证医学证据分级系统,综合考虑临床实际及专科医师经验,形成最终的影像组学模型推荐意见,以期为临床医师明确诊断提供方法学依据,形成最佳决策方案,指导临床综合治疗。

关键词：肝细胞癌诊断组学模型共识

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中国儿童百日咳诊断及治疗建议

中华儿科杂志 2017年第8期55卷 568-572页

作者：邓继岿深圳市儿童医院感染科 518038

百日咳是一种具有高度传染性的急性呼吸道疾病,其特征性临床症状为阵发性痉挛性咳嗽伴吸气“鸡鸣”样回声,病程可迁延数月,常引起流行.近年来,儿童百日咳发病率逐年升高,并出现一些新的特征,百日咳已经成为儿科临床医师广泛关注的传染... 详细信息

百日咳是一种具有高度传染性的急性呼吸道疾病,其特征性临床症状为阵发性痉挛性咳嗽伴吸气“鸡鸣”样回声,病程可迁延数月,常引起流行.近年来,儿童百日咳发病率逐年升高,并出现一些新的特征,百日咳已经成为儿科临床医师广泛关注的传染性疾病.在我国,有关儿童百日咳的诊断方法和标准、抗菌药物的使用和疗程、对症处理等诸多问题亟待规范.为指导临床医生正确认识百日咳,现就儿童百日咳的诊断及治疗建议如下.

关键词：中国儿童诊断方法百日咳治疗急性呼吸道疾病传染性疾病痉挛性咳嗽临床症状

Expression of Hepatitis C Virus E2 Ectodomain in *** and Its Application in the Detection of Anti-E2 Antibodies in Human Sera

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 57-63页

作者： Jing LIU, Xin-Xin ZHANG, Shen-Ying ZHANG, Min LU, Yu-Ying KONG, Yuan WANG, and Guang-Di LI ( State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the chinese Academy of Sciences, Shanghai 200031, China 2 Department of infectious diseases, Ruijin Hospital, Second medical University of Shanghai, Shanghai 200025, China ) State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 Chin

The second envelope glycoprotein （E2） of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However, large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment （aa 385-730） with a four-amino-acid mutation （aa 568-571 PCNI to RVTS） was expressed as hexa-histidine-tagged full length protein [E2N730（m）] in E. coli and purified to over 85% purity. Purified E2N730（m） was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2specific antigenicity. Rabbit antiserum raised against E2N730（m） recognized E2 glyco-proteins expressed in mammalian cells in Western blot. Purified E2N730（m） was used to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previousl

关键词： hepatitis C virus envelope protein E2 expression and purification Escherichia coll

Expert Consensus on Polymyxin Antimicrobial Susceptibility Testing and Clinical Interpretation

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chinese medical Sciences Journal 2021年第1期36卷 1-16页

作者： Qiwen Yang Xiaoling Ma Fupin Hu Jing Zhang Tongwen Sun Baiyi Chen Yingchun Xu Youning Liu chinese Committee on Antimicrobial Susceptibility Testing(ChiCAST) Expert Committee on infectious diseases,China medical Education a.socia.ion Department of Clinical Laboratory State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Department of Laboratory Medicine the First Affiliated HospitalUniversity of Science and Technology of ChinaHefei 230001China lnstitute of Antibiotics Huashan HospitalFudan UniversityShanghai 200040China General ICU the First Affiliated HospitalZhengzhou UniversityZhengzhou 450052China Department of infectious diseases the First Affiliated HospitalChina Medical UniversityShenyang 110001China Department of Respiratory Medicine the First Medical Center of PLA General HospitalBeijing 100853China 不详

The polymyxins are important antimicrobial agents against antibiotic-resistant gram-negative *** 2020,the Clinical and Laboratory Standards Institute modified the clinical breakpoints for polymyxin susceptibility test by eliminating the"susceptible"interpretive category,only reporting intermediate(≤2 mg/L)and resistant(≥4 mg/L).However,the European Committee on Antimicrobial Susceptibility Testing recommended the use of clinical breakpoints of W2 mg/L as susceptible and>2 mg/L as *** first-line laboratorians and clinicians in China have been perplexed by the inconsistence of international polymyxin clinical breakpoints and discouraged by the difficulty of conducting polymyxin susceptibility ***,it is urgently needed to make it clear for the laboratorians in China to know how to accurately carry out polymyxin susceptibility testing and standardize the interpretation of susceptibility testing *** this end,the experts from relevant fields were convened to formulate this consensus statement on the testing and clinical interpretation of polymyxin *** recommendations are proposed accordingly for laboratorians and clinicians to streamline their daily work.

关键词： polymyxin antimicrobial susceptibility testing clinical interpretation expert consensus

2024年3月中国甲乙丙类传染病疫情动态概要

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疾病监测 2024年第4期39卷 390-391页

作者：中国疾病预防控制中心传染病预防控制处中国疾病预防控制中心传染病预防控制处

2024年3月(2024年3月1日00:00至3月31日24:00),除新型冠状病毒感染外,全国(不含香港特别行政区、澳门特别行政区和台湾省,下同)共报告法定传染病1450149例,死亡2329例。其中,甲类传染病未报告发病、死亡病例。乙类传染病中传染性非典型... 详细信息

2024年3月(2024年3月1日00:00至3月31日24:00),除新型冠状病毒感染外,全国(不含香港特别行政区、澳门特别行政区和台湾省,下同)共报告法定传染病1450149例,死亡2329例。其中,甲类传染病未报告发病、死亡病例。乙类传染病中传染性非典型肺炎、脊髓灰质炎、人感染高致病性禽流感、流行性乙型脑炎、白喉、新生儿破伤风和人感染H7N9禽流感无发病、死亡报告,除新型冠状病毒感染外,其余20种乙类传染病共报告发病368244例,较上月(275681例)上升34%,较去年同期(307520例)上升20%。报告发病数居前5位的病种依次为病毒性肝炎、肺结核、梅毒、百日咳和淋病,占乙类传染病报告病例总数的95%。报告死亡2325例,较上月(1674例)上升39%(651例),较去年同期(2354例)下降1%(29例)。

关键词：新生儿破伤风乙类传染病病例总数死亡报告报告发病数脊髓灰质炎甲类传染病疫情动态

2024年4月中国甲乙丙类传染病疫情动态概要

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疾病监测 2024年第5期39卷 522-523页

作者：中国疾病预防控制中心传染病预防控制处中国疾病预防控制中心传染病预防控制处

2024年4月(2024年4月1日00:00至4月30日24:00),除新型冠状病毒感染外,全国(不含香港地区、澳门特别行政区和台湾省,下同)共报告法定传染病1120260例,死亡2290例。其中,甲类传染病未报告发病、死亡病例。乙类传染病中传染性非典型肺炎、... 详细信息

2024年4月(2024年4月1日00:00至4月30日24:00),除新型冠状病毒感染外,全国(不含香港地区、澳门特别行政区和台湾省,下同)共报告法定传染病1120260例,死亡2290例。其中,甲类传染病未报告发病、死亡病例。乙类传染病中传染性非典型肺炎、脊髓灰质炎、人感染高致病性禽流感、白喉、新生儿破伤风和人感染H7N9禽流感无发病、死亡报告,除新型冠状病毒感染外,其余21种乙类传染病共报告发病420216例,较上月(368244例)上升14%,较去年同期(291128例)上升44%。报告发病数居前5位的病种依次为病毒性肝炎、百日咳、肺结核、梅毒和淋病,占乙类传染病报告病例总数的95%。报告死亡2290例,较上月(2325例)下降2%(减少35例),较去年同期(2185例)上升5%(增加105例)。

关键词：新生儿破伤风人感染高致病性禽流感乙类传染病病例总数死亡报告报告发病数脊髓灰质炎甲类传染病