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Cell Research 1992年第2期2卷 129-137页

作者： DAI JIANWU, QUN DONG, YAXIANSU,AND KECHUN LIN department of Cell biology & biophysics, Beijing Medical University, Beijing 100083, china department of biophysics Beijing Medical University Beljing 100083 China

Recently,the effect of ligand receptor interaction onthe membrane structure of liposomes has been studied ***,little is known about how it exists onbiological *** this paper,the effect of Con-canavalin A(ConA)receptor interaction on the structure ofcell membranes was studied by Circular Dichroism(CD)and31P Nuclear Magnetic Resonance(NMR). CD results of both the purified macrophage membranesand human erythrocyte ghosts(EG)showed that the con-formation of membrane proteins changed after ConA bind-ing. For further research,31P-NMR was used to detect theorganization of phospholipid molecules on macrophage *** ConA binding,the tendency to form non bi-layer structure increased with the amount of *** of 31P-NMR spectra of living macrophages mightbe partly due to the above stated reason too. In addition,ConA-receptor interaction also inducedsimilar results of 3lP-NMR spectra in *** contrast,wheat germ agglutinin(WGA),another kind of lectin,rarely showed the same influence.

关键词： ConA macrophage e.rythrocyte ghost CD 31P NMR non bilayer lipid structure.

Preliminarily Investigating the Polymorphism of Self-organized Actin Filament in Vitro by Atomic Force Microscope

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 637-643页

作者： Jun ZHANG1,2, Yuan-Liang WANG1*, Xin-Yong Chen3, Chuang-Long HE1, Chao CHENG1, and Yang CAO1 1Key Lab of Biomechanics & Tissue Engineering, Chongqing University, Chongqing 400044, china biology department, Chongqing University of Medicine Sciences, Congqing 400016, china 3Laboratory of biophysics and Surface Analysis, University of Nottingham, Nottingham NG7 2RD, United Kingdom Key Lab of Biomechanics & Tissue Engineering Chongqing University biology department Chongqing University of Medicine Sciences Laboratory of biophysics and Surface Analysis University of Nottingham

With the atomic force microscope (AFM), we preliminarily investigated the large-scale structure of actin filaments formed in low concentration protein solution (5 μg/ml) via self-organization without the presence of any F-actin dynamic interfering factors (such as phalloidin) in vitro. It was found that the G-actin could be polymerized into ordered filamentous structures with different diameter from the slimmest filament of single F-actin to giant filament in tree-like branched aggregates. The observed polymerized actin filaments, to which our most intense attention was attracted, was discretely distributed and showed obvious polymorphism distinctly different from those in the presence of phalloidin or actin binding proteins (fimbrin, gelsolin, etc.) in previous experiments. Latter structures were mainly composed of single F-actin and/or multifilaments clearly consisting of several single F-actin. The experimental results clearly demonstrated that non-interference with the F-actin intrinsic dynamics in self-organizing could lead to the polymorphism of actin filamentous structures, and further analysis implied that the disturbance of normal F-actin dynamics by many factors could prevent the emergence of structural polymorphism, more often than not, give rise to formation of specific structures instead and different interference would bring about various particular structures under certain conditions.

关键词： actin self-organization filamentous structure polymorphism atomic force microscope

Structural immunology of costimualtory and coinhibitory molecules

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Science china(Life Sciences) 2010年第2期53卷 183-189页

作者： WANG ShengDian1 & CHEN LiePing1,2 1Center for Infection and Immunity,Institute of biophysics,Chinese Academy of Sciences,Beijing 100101,china 2department of Oncology,Johns Hopkins University School of Medicine,Baltimore,Maryland,USA 1. Center for Infection and Immunity Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China2. Department of Oncology Johns Hopkins University School of Medicine Baltimore Maryland USA

The T cell costimulatory pathways are central to regulating immune responses,and targeting these pathways represents one of the most promising approaches for achieving *** molecular structures of costimulation revealed invaluable mechanistic insights underlying costimulatory receptor/ligand specificity,affinity,oligomeric state,and valency,which provided the bases for better manipulation of these signaling *** incredible growth of this field led to identification of new members and unexpected interactions,revealing a complicated regulatory network of immune *** advances in structural biology of costimulation will promise unprecedented opportunities for furthering our understanding and therapeutic application of T cell costimulatory pathways.

关键词： costimulatory molecules T cells molecular structure immunotherapy

Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat

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Cell Research 2004年第2期14卷 161-168页

作者： Kai CHEN~(1,*), Dan LI~(1,*), Yu Hui JIANG~1, Wei Juan YAO~1, Xin Juan WANG~2, Xiao Chao WEI~2, Jing GAO~3, Li De XIE~1, Zong Yi YAN~4, Zong Yao WEN~(1**), Shu CHIEN~5 ~1Hemorheology Center, department of biophysics, School of Basic Medical Sciences, Peking University, Beijing, 100083, china. ~2department of Biochemical and Molecular biology, School of Basic Medical Sciences, Peking University, Beijing, 100083, china. ~3department of Urology, Medical Center, New York University, USA. ~4department of Mechanics and Engineering Science, Peking University, Beijing 100871, china. ~5department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0412, USA 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 2department of Biochemical and Molecular biology School of Basic Medical Sciences Peking University Beijing 100083 China. 2department of Biochemical and Molecular biology School of Basic Medical Sciences Peking University Beijing 100083 China. 3department of Urology Medical Center New York University USA. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 4department of Mechanics and Engineering Science Peking University Beijing 100871 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 5department of Bioengineering and Whitaker Institute of Biomedical Engineering University of California San Diego La Jolla CA 92093-0412 USA

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K_I increased, and the proportion of α-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on

关键词： TRAIL Tet gene expression system Jurkat apoptosis.

The developmental expression of Glial fibrillary acidic protein（GFAP）,neurofilament and vimentin in postnatal mouse retina

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The developmental expression of Glial fibrillary acidic prot...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Huan-Ling YAO~(1,2),Shi-Gang HE~1 1 Institute of biophysics,china Academy of Science,Beijing 100101 2.department of biology,Henan University,Kaifeng 475001

＜正＞Purpose To investigate the mouse retinal structure during postnatal development with some antibodies specific for glial fibrillary acidic protein（GFAP）,Vimentin which were considered as glial cells specific biomarkers,and Neurofilament triplet,an intermediate filament protein in *** The location of the expression of these proteins was studied in different stage mice（from Postnatal day 0 to Adults） by means of immunofluorescence double-labeled in retina cryosections and *** GFAP was mainly expressed in astrocytes located in inner limited membrane of mouse retina and Neurofilament was expressed in retina ganglion cells’ axon and in part of retina ganglion cells’ bodies from postnatal day 0（P0） to adult *** the above results,we have also found that GFAP and Neurofilament were co-expressed in some cells’ body and their processes located in prospective outer plexiform layer（OPL） of mouse retina from P0 to P3 and co-located in OPL from P5 to *** was expressed in M（?）ller cells’ processes,especially in their endfoot in adult mouse *** results were observed in P8 and P13 mouse *** GFAP and Vimentin were fasciatedly co-located in part of Muller cells’ processes in adult mouse *** Our results supported the former study that GFAP was mainly expressed in astrocytes and Vimentin was expressed in radial glail cells,but GFAP as an astrocytes marker should be *** cells co-expressed GFAP and Neurofilment in P0 to P3 mouse retina maybe horizontal cells and its testifying needs further study.

关键词： mouse retina development glial fibrillary acidic protein neurofilament vimentin

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微囊蛋白基因及其与疾病关系研究进展

生物化学与生物物理进展 2003年第3期30卷 375-378页

作者：梁旭方黄芬暨南大学生物工程学系广州510632 中国科学院生物物理研究所生物大分子国家重点实验室北京100101

微囊蛋白 (caveolin)基因家族已鉴定出 3个成员 :微囊蛋白 1、微囊蛋白 2、微囊蛋白 3 ,并被定位于抑癌基因位点区域 .微囊蛋白 1是细胞质膜微囊的标记蛋白 ,其中间疏水区域在细胞膜内形成发夹结构 ,并使其N端区域与C端区域在细胞膜内... 详细信息

微囊蛋白 (caveolin)基因家族已鉴定出 3个成员 :微囊蛋白 1、微囊蛋白 2、微囊蛋白 3 ,并被定位于抑癌基因位点区域 .微囊蛋白 1是细胞质膜微囊的标记蛋白 ,其中间疏水区域在细胞膜内形成发夹结构 ,并使其N端区域与C端区域在细胞膜内表面聚合形成支架结构 .微囊蛋白 1与微囊蛋白 2以组成异源寡聚体的形式存在 ,在脂肪细胞、内皮细胞和成纤维细胞中表达最丰富 ,微囊蛋白 3则特异表达于肌肉 .离体与活体研究结果均表明微囊蛋白 1可能具有抑癌功能 ,并可能在细胞信号传导中起刹车作用 .微囊蛋白 1基因敲除小鼠心血管NO与Ca2 +信号途径受损、功能异常 ,肺泡上皮细胞出现异常扩增 ,脂质代谢失衡 ,身体消瘦 .微囊蛋白 2基因敲除小鼠肺功能与耐力均严重受损 ,与微囊蛋白 1基因敲除小鼠的表型非常相似 .微囊蛋白 3为维持心脏正常功能所必需。

关键词：微囊蛋白基因疾病信号转导生物膜细胞质膜微囊

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芋螺毒素研究进展

生物化学与生物物理进展 2003年第4期30卷 537-545页

作者：王承忠蒋辉戚正武中国科学院上海生命科学研究院生物化学与细胞生物学研究所

芋螺毒素是近年来国际上的一个研究热点 .它属于一类海洋生物活性多肽 ,多数由 12～ 4 6个氨基酸残基所组成 ,能特异性地作用于体内各种离子通道和细胞膜上神经递质和激肽的受体 .具有分子质量小、结构多样、作用靶点广泛、功能专一、... 详细信息

芋螺毒素是近年来国际上的一个研究热点 .它属于一类海洋生物活性多肽 ,多数由 12～ 4 6个氨基酸残基所组成 ,能特异性地作用于体内各种离子通道和细胞膜上神经递质和激肽的受体 .具有分子质量小、结构多样、作用靶点广泛、功能专一、组织特异性强等优点 ,因而较之其他生物来源的活性多肽有更多的优越性 .芋螺毒素是一待挖掘的“富矿区” ,可作为体内一些具有重要生理功能靶点的探针和“解密器” ,亦可作为新药的先导化合物或直接开发成新药。

关键词：芋螺毒素活性多肽离子通道神经递质拮抗剂激动剂

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昆虫神经肽研究进展

生物化学与生物物理进展 1997年第2期24卷 116-120页

作者：徐卫华中国科学技术大学生物系合肥230026

近年来鉴定了化学结构的昆虫神经肽数目呈快速上升趋势 ,家蚕滞育激素和性信息素合成激活肽被分离纯化 .三种近年出现的研究方法对寻找新型昆虫神经肽起到重要作用 ,已经成功地鉴定了数个新型神经肽 .

关键词：昆虫神经肽发育

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真核生物DNA非编码区的组分分析

生物化学与生物物理进展 2002年第4期29卷 583-587页

作者：刘蓉齐震朱小蓬凌伦奖韩汝珊北京大学物理系北京100871 中国科学院生物物理研究所北京100101

在全基因组水平上 ,用直方图、混沌表示灰度图、距离差异度和信息熵差异度四种方法 ,研究了拟南芥、线虫、果蝇的DNA内含子、基因间隔区DNA、外显子三种区域的核苷酸短序列组分及组分复杂度 .结果表明 :a 不同基因组之间 ,不管基因数目... 详细信息

在全基因组水平上 ,用直方图、混沌表示灰度图、距离差异度和信息熵差异度四种方法 ,研究了拟南芥、线虫、果蝇的DNA内含子、基因间隔区DNA、外显子三种区域的核苷酸短序列组分及组分复杂度 .结果表明 :a 不同基因组之间 ,不管基因数目多少 ,用 4种方法得到的外显子部分其组分复杂度都比较接近 ,而非编码区部分的组分复杂度却很大 .这一点定量地说明了物种之间的复杂程度 ,主要不体现在编码区部分 ,而体现在非编码区部分 .b 同一基因组中 ,内含子的核苷酸短序列组分复杂度都是相似的 ,外显子和intergenicDNA部分的组分复杂度也是相似的 .c 内含子和intergenicDNA在转录、剪切、二级结构等方面有很大的不同 ,但它们在核苷酸短序列组分上的差异却很小 ,说明内含子和intergenicDNA在转录、剪切、二级结构上的不同并不通过核苷酸短序列组分来进行限制 .

关键词：真核生物 DNA非编码区组分分析