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中华微生物学和免疫学杂志 2003年第1期23卷 3-4页

作者： YAN Zi-hou Chengdu biological products institute, Chengdu 610063, china Chengdu biological products institute Chengdu 610063

*** Liang was born in Chengdu, Sichuan province in 1891. Since graduation from Hanoi Medical College, Vietnam, in 1913 seeing quite a lot of people including his brother and sister died of tuberculosis (TB), he made h... 详细信息

关键词：卡介苗中国人物传记疫苗

Studies on Determining Method of Trehalose

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Chemical Research in Chinese Universities 2000年第1期16卷 10-14页

作者： LIU Tie-min (State Key Laboratory of Enzyme Engineering, Jilin University, Changchun 130023, P. R. china) LIU Ying (Changchun institute of biological products, Changchun 130062, P. R. china) Jilin Univ State Key Lab Enzyme Engn Changchun 130023 Peoples R China Changchun Inst Biol Prod Changchun 130062 Peoples R China

Trehalose can be determined by thin layer chromatography and high performance liquid chromatography. But it's difficult to distinguish trehalose from the similar bisaccharides. If the reaction of the reduced bisaccharides with phenyl hydrazine to form osazone is used, then the disturbance of the similar reduced bisaccharides can be rapidly and conveniently excluded, or via the ammoniated-reduced reaction, aliline can be specially bounded to the reduced hydroxyl of the bisaccharides. In that way trehalose can be accurately determined.

关键词： Trehalose Thin layer chromatography High performance liquid chromatography Osazone Ammoniated-reduced reaction

Stem cells and neurodegenerative diseases

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Science china(Life Sciences) 2008年第4期51卷 287-294页

作者： HOU LingLing & HONG Tao institute of biological Science and Technology, Beijing Jiaotong University, Beijing 100044, china 1. institute of biological Science and Technology Beijing Jiaotong University Beijing 100044 China

Neurodegenerative diseases are characterized by the neurodegenerative changes or apoptosis of neurons involved in networks, which are important to specific physiological functions. With the de-velopment of old-aging society, the incidence of neurodegenerative diseases is on the increase. How-ever, it is difficult to diagnose for most of neurodegenerative diseases. At present, there are too few effective therapies. Advances in stem cell biology have raised the hope and possibility for the therapy of neurodegenerative diseases. Recently, stem cells have been widely attempted to treat neurodegen-erative diseases of animal model. Here we review the progress and prospects of various stem cells, including embryonic stem cells, mesenchymal stem cell and neural stem cells and so on, for the treatments of neurodegenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, Hunt-ington’s disease and Amyotrophic lateral sclerosis/Lou Gehrig’s disease.

关键词： stem cells neurodegenerative diseases therapy

Axon guidance and neuronal migration research in china

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Science china(Life Sciences) 2010年第3期53卷 304-314页

作者： YUAN XiaoBing institute of Neuroscience,Shanghai institutes for biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china 1. institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Proper migration of neuronal somas and axonal growth cones to designated locations in the developing brain is essential for the assembly of functional neuronal *** progress in research of axon guidance and neuronal migration has been made in the last twenty *** researchers began their exploration in this field ten years ago and have made significant contributions in clarifying the signal transduction of axon guidance and neuronal *** unique experimental approaches,including the migration assay of single isolated neurons in response to locally delivered guidance cues,have been developed by Chinese neuroscientists to investigate the molecular machinery underlying these guidance events.

关键词： axon guidance neuronal migration development neuronal circuits

The background of the total synthesis of yeast alanine transfer RNA

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Science china(Life Sciences) 2010年第1期53卷 19-21页

作者： QI GuoRong institute of Biochemistry and Cell Biology,Shanghai institute for biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china 1. institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The research findings concerning the total synthesis of yeast alanine transfer RNA (yeast alanine tRNA) were successively published in Chinese Science Bulletin (1982) and Science

关键词： tRNA The background of the total synthesis of yeast alanine transfer RNA

Conus Peptides-A Rich Pharmaceutical Treasure

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 713-723页

作者： Cheng-Zhong WANG and Cheng-Wu CHIinstitute of Biochemistry and Cell Biology, Shanghai institute of biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, china institute of Neuroscience, the Chinese Academy of Sciences, Shanghai 200031, china institute of Protein Research, Tongji University, Shanghai 200092, china institute of Biochemistry and Cell Biology Shanghai Institute of Biological Sciences the Chinese Academy of Sciences institute of Neuroscience the Chinese Academy of Science institute of Protein Research Tongji University

Marine predatory cone snails (genus Conus) with over 500 species represent what is arguably the largest single genus of marine animals alive today. All Conus are venomous and utilize a complex mixture of Conus peptide... 详细信息

关键词： Conus peptide conotoxin neuropathic disorder pharmaceutical potency

The topological configuration and conformational analysis of mRNA in translation

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Chinese Science Bulletin 2002年第5期47卷 384-389页

作者： LIU Shuqun & LIU CiquanKunming institute of Zoology, Chinese Academy of Sciences, Kunming 650223, china Modern biological Center, Yunnan University, Kunming 650091, china Kunming institute of Zoology Chinese Academy of Sciences Kunming China Modern biological Center Yunnan University Kunming China

The theoretical model construction of mRNA hairpin structure and single-stranded structure as well as the simulation studies on RNA structure determined by the X-ray crystal diffraction and nuclear magnetic resonance revealed that in translation, after mRNA being unfolded into single-stranded structure, its topological configuration was closely correlative with the original hairpin structure. The conformational features of single-stranded mRNA appeared as helical regions alternating with curly regions to different extents, which might exert the influence on the folding of nascent polypeptide by various regulating effects including different translational rates.

关键词： hairpin structure of mRNA topological configuration of single-stranded mRNA.

The first protein ever synthesized in vitro—a personal reminiscence of the total synthesis of crystalline insulin

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Science china(Life Sciences) 2010年第1期53卷 16-18页

作者： ZHANG YouShang institute of Biochemistry and Cell Biology,Shanghai institutes for biological Sciences,Chinese Academy of Sciences Shanghai 200031,china 1. institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The total synthesis of crystalline bovine insulin started in 1958 was fully accomplished in 1965. This formidable task was a collaboration of the institute of Biochemistry in Shanghai,

关键词： The first protein ever synthesized in vitro a personal reminiscence of the total synthesis of crystalline insulin

Enhanced activity of yqhD oxidoreductase in synthesis of 1,3-propanediol by error-prone PCR

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Progress in Natural Science:Materials International 2008年第12期18卷 1519-1524页

作者： Hongmei Li~a Jia Chen~a Yinghua Li~b a institute of Food Science and biological Technology,University of Shanghai for Science and Technology,Shanghai 200093,china b institute of Pharmaceutical Engineering,Zhejiang University,Hangzhou 310027,china [a]institute of Food Science and biological Technology University of Shanghai for Science and Technology Shanghai 200093 China [b]institute of Pharmaceutical Engineering Zhejiang University Hangzhou 310027 China

yqhD oxidoreductase was determined to be an NADP-dependent dehydrogenase,and was more active toward 3-HPA when compared to 1,3-propanediol *** further improve enzyme activity towards 3-hydroxypropionaldehyde (3-HPA),error-prone PCR was implemented to mutant yqhD *** mutants,D99QN147H and Q202A with increased catalytic and affinity efficiency,were obtained after one round of error-prone polymerase chain *** the catalytic efficiency of the mutant D99QN147H was up to 4-fold greater than the wild enzyme (0.0375 min-1mM-1vs.0.0078 min-1mM-1).The recombined strain containing pEY28yqhD D99QN147H yielded 28 g L-1of 1,3-propanediol in the fed-batch LB cultures (1 L volume) with an initial 3-HPA concentration of 40 g L-1,which was higher than the 21 and 17 g L-1of 1,3-propanediol from the mutant Q202A and the wild-type,*** for propionaldehyde,the optimal mutant D99QN147H also exhibited higher activity on a range of substituted aldehydes than the wild- type.

关键词： yqhD oxidoreductase Error-prone PCR Catalytic efficiency 1,3-Propanediol