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Proteomic Analysis of Cucumber Defense Rresponses Induced by Propamocarb

Journal of Integrative Agriculture 2013年第11期12卷 2022-2035页

作者： wu Peng QIN Zhi-wei wu Tao ZHOU Xiu-yan XIN Ming GUO Qian-qian College of Horticulture Northeast Agricultural University

Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes （glutathione reductase, GR; glutathione S-tramsferase, GST） and soluble sugar content of cucumber fruit were signiifcantly increased, but malonaldehyde （MDA） content was signiifcantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis （2-DE） protein proifles of control and propamocarb-treated fruits, and identiifed 18 differentially expressed （13 up-regulated and 5 down-regulated） proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-lfight mass spectrometry （MALDI-TOF-MS）. The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction （qRT-PCR） was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.

关键词： cucumber fungicide propamocarb proteomics qRT-PCR

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Evaluation of spontaneous combustion tendency of sulfide ore heap based on nonlinear parameters

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Journal of Central South University 2017年第10期24卷 2431-2437页

作者： PAN Wei wu Chao LI Zi-jun wu Zhi-wei YANG Yue-ping School of Resources and Safety Engineering Central South University School of Nuclear Resources Engineering University of South China

To explore a new evaluation method for spontaneous combustion tendency of different areas in sulfide ore heap, ore samples from a pyrite mine in China were taken as experimental materials, and the temperature variations of the measuring points of simulated ore heap were measured. Combined with wavelet transform and nonlinear parameters extraction, a new method for spontaneous combustion tendency of different areas in sulfide ore heap based on nonlinear parameters was proposed and its reliability was verified by field test. The results indicate that temperature field evolution of the simulated ore heap presents significant spatial difference during self-heating process. Area with the maximum increasing extent of temperature in sulfide ore heap changes notably with the proceeding of self-heating reaction. Self-heating of sulfide ore heap is a chaotic evolution process, which means that it is feasible to evaluate spontaneous combustion tendency of different areas by nonlinear analysis method. There is a relatively strong correlation between the maximum Lyapunov exponent and spontaneous combustion tendency with the correlation coefficient of 0.9792. Furthermore, the sort of the maximum Lyapunov exponent is consistent with that of spontaneous combustion tendency. Therefore, spontaneous combustion tendency of different areas in sulfide ore heap can be evaluated by means of the maximum Lyapunov exponent method.

关键词： sulfide ore heap spontaneous combustion tendency self-heating process nonlinear parameters maximum Lyapunov exponent

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Analysis of Stock Splits Based on Risk Theory: Empirical Evidence from the Chinese Stock Markets

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Journal of Systems Science and Information 2022年第1期10卷 19-34页

作者： Shujin wu Tong XU MOE Key Laboratory of Advanced Theory and Application in Statistics and Data Science School of StatisticsEast China Normal UniversityShanghai 200062China School of Statistics East China Normal UniversityShanghai 200062China

The paper first analyzes price change due to stock splits in Chinese stock markets,which shows stock prices typically go up for stock *** theoretical analyses based on risk theory are presented to explain the reason,where the method comes from a new perspective and obtained theoretical conclusions show that stock splits typically make stock price go up if risk-compensation function is convex,and go down if risk-compensation function is *** prices typically go up for stock splits because risk-compensation functions are mainly *** obtained conclusions are consistent with the known results in the last three decades.

关键词： stock split risk theory price Chinese stock markets

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Evaluating Local Elasticity of the Metal Nano-films Quantitatively Based on Referencing Approach of Atomic Force Acoustic Microscopy

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Chinese Journal of Mechanical Engineering 2012年第6期25卷 1281-1286页

作者： ZHANG Gaimei HE Cunfu wu Bin CHEN Qiang College of Mechanical Engineering and Applied Electronics Technology Beijing University of Technology Beijing 100124 China Laboratory of Plasma Physics and Materials Beijing Institute of Graphic Communication Beijing 102600 China

Traditional technique such nanoindenter（NI） can＇t measure the local elastic modulus at nano-scale（lateral）. Atomic force acoustic microscopy （AFAM） is a dynamic method, which can quantitatively determine indentation modulus by measuring the contact resonance spectra for high order modes of the cantilever. But there are few reports on the effect of experimental factors, such length of cantilever, contact stiffness on measured value. For three different samples, including copper（Cu） film with 110 nm thickness, zinc（Zn） film of 90 nm thickness and glass slides, are prepared and tested, using referencing approach in which measurements are performed on the test and reference samples （it＇s elastic modulus is known）, and their contact resonance spectra are measured used the AFAM system experimentally. According to the vibration theory, from the lowest two contact resonance frequencies, the tip-sample contact stiffness is calculated, and then the values for the elastic properties of test sample, such as the indentation modulus, are determined. Using AFAM system, the measured indentation modulus of copper nano-film, zinc nano-film and glass slides are 113.53 GPa, 87.92 GPa and 57.04 GPa, which are agreement with literature values Mcu--105-130 GPa, Mzn = 88.44 GPa and Molass = 50-90 GPa. Furthermore, the sensitivity of contact resonance frequency to contact stiffness is analyzed theoretically. The results show that for the cantilevers with the length 160 pm, 225 μm and 520 μm respectively, when contact stiffness increases from 400 N/m to 600 N/m, the increments of first contact resonance frequency are 126 kHz, 93 kHz and 0.6 kHz, which show that the sensitivity of the contact resonance frequency to the contact stiffness reduces with the length of cantilever increasing. The novel method presented can characterize elastic modulus of near surface for nano-film and bulk material, and local elasticity of near surface can be evaluated by optimizing the experimental parameters using

关键词： local elasticity atomic force acoustic microscope(AFAM) contact stiffness referencing approach

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Two-dimensional Conductive Metal-Organic Framework Nanowire Arrays for Highly Performed Soft Electrochemical Actuators

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材料导报 2021年第9期35卷 9001-9002页

作者： ZHAO Ruoqing wu Hao WEI Yuan WANG Xuewen Frontiers Science Center for Flexible Electronics(FSCFE)&Shaanxi Institute of Flexible Electronics(SIFE) Northwestern Polytechnical UniversityXi’an 710072China

Fig.1(a)Schematic illustration of the fabrication of a Ni-CAT NWAs/CNF hybrid membrane by the in situ hydrothermal growth of Ni-CAT NWAs on CNF;SEM images of(b)the pristine CNF nanomembrane and(c)Ni-CAT NWAs/CNF,and(d)size distribution statistics of the diameter of Ni-CAT NWAs/CNF;schematic illustrations of(e)the fabrication procedure for the Ni-CAT NWAs/CNF-based actuator,(f)Ni-CAT NWAs/CNF electrode surfaces and Ni-CAT along the c-axis(Color codes:O,red spheres;C,gray spheres;and Ni,blue spheres);(g)actuation performance of the Ni-CAT NWAs/CNF based actuator The demand for high-performance low-voltage driven electromechanical actuators is growing because of their potential applications,such as in soft robotics,artificial muscles,biomimetic flying insects,and micro/nano-electromechanical systems[1].Among these actuators,ionic-polymer metal composite actuators(IPMCs)are promising as they are capable of large actuation deformation under low operation voltages(only a few volts),and they operate best in a humid environment[2].However,although extensive efforts have been made in past decades,developing high-capacitance electrode materials that improve the performance of IPMCs is challenging.

关键词： composite illustration spheres

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The PPR protein PDM1 is involved in the processing of rpoA pre-mRNA in Arabidopsis thaliana

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Chinese Science Bulletin 2010年第30期55卷 3485-3489页

作者： wu Hao ZHANG LiXin School of Life Sciences Lanzhou University Lanzhou 730000 China Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Pentatricopeptide repeat (PPR) proteins,containing tandem repeats of degenerate 35 amino acid motifs,are important for post-transcriptional chloroplast gene expression. In this study,we report the characterization of a pigment-deficient mutant 1 (pdm1) in Arabidopsis,which displays the albino phenotype. PDM1 contains 5 PPR motifs followed by a PLS domain. The levels of plastid-encoded polymerase-dependent chloroplast genes were reduced dramatically,whereas those of nucleus-encoded polymerase-dependent chloroplast genes increased in the mutant. In addition,the pattern of rpoA pre-mRNA was altered and the rpoA transcript was absent in pdm1. Thus,these results suggest that PDM1 is required for processing of rpoA pre-mRNA in Arabidop-sis.

关键词：基因表达拟南芥 PR蛋白前处理叶绿体基因缺失突变体串联重复序列薪酬水平

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Synthesis and characterization of monoclinic TiO_(2) nanosheets

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Chinese Science Bulletin 2005年第19期50卷 2153-2156页

作者： wu Yu XU Boqing Innovative Catalysis Program Key Lab of Organic Optoelectronics &Molecular Engineering Department of Chemistry Tsinghua UniversityBeijing 100084 China

A novel two-step method for the synthesis of monoclinic titanium oxide (i.e. TiO2(B)) nanosheets is pre-sented in this report. The method is featured by two steps: 1) synthesis of hydrogen titanate nanosheets, followed by 2) calcination of the titanate nanosheets at elevated tempera-tures. The hydrogen titanate nanosheets were prepared first by autoclaving anatase TiO2 powders, obtained by air cal-cining an ethanol-gel of Ti(OH)4 at 500℃, in aqueous NaOH (10 mol/L) at 150―200℃, and then by washing with hydro-chloric acid under supersonic irradiation. While sizes of the nanosheets were found to increase with increasing the tem-perature of the hydrothermal treatment, the calcination at 400―500℃ of the hydrogen titanate nanosheets that were synthesized at higher autoclaving temperatures (180―200℃) produced monoclinic TiO2 nanosheets with a uniform mor-phology. By contrast, the same calcination of the titanate nanosheets synthesized at the autoclaving temperature 180℃ led to anatase TiO2 nanoparticles.

关键词：二氧化钛纳米材料单斜晶薄片超声照射纳米离子

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Etiological study for a case of multi-bacterial synergistic gangrene

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Chinese Science Bulletin 1997年第6期42卷 511-517页

作者： Xu, JG Yang, HM Lai, XH Fu, XL wu, JM Huang, LB Yu, XJ wu, YP wu, YJ Liu, BY Priority Laboratory of Molecular Medical Bacteriology Ministry of Health Institute of Epidemiology and Microbiology Chinese Academy for Preventive Medicine Beijing China Laboratory of Bacteriology Beijing Army General Hospital Beijing China

IN July of 1994, a 13-year old girl from Shandong Province, China, found a dark point on her middle finger of the right hand. She licked it with a needle, and washed clothes with pool water by hands afterwards. Intolerable pain was experienced about 10h later. The gangrene was developed in a week and spread gradually up to the right forearm and two fingers of the left hand. In November of 1994, she visited Beijing Army General Hospital, and radical amputations of the right forearm and two fingers of the left hand were carried out after 3d of

关键词： gangrene anaerobes antibiotics.

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More or less is fine:an undercover work of DKK1 in anthrax toxin uptake

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Science China(Life Sciences) 2014年第7期57卷 735-736页

作者： MAO BingYu wu Wei State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of Sciences School of Life Sciences Tsinghua University

The protective antigen(PA)mediated internalization of the anthrax complexes is critical in the development of anthrax,which depends on specific membrane receptors,namely ANTXR1(also known as TEM8)and ANTXR2(also known as CMG2)[1].In a screen for host proteins required for anthrax toxin action,low-density lipoprotein receptor-related protein 6(LRP6)was found important for efficient toxin entry in human M2182 prostate carcinoma cells[2],which interacts with both ANTXR1 and ***,the role of LRP6 in anthrax toxicity remains controversial as both in mice and in human HeLa cells,knockout or knockdown of LRP6 has no clear effect on their sensitivity to anthrax toxins[3].

关键词：炭疽毒素卧底吸收前列腺癌细胞受体相关蛋白低密度脂蛋白 HeLa细胞保护性抗原

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Has the cardiac stem cell controversy settled down?

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Science China(Life Sciences) 2014年第9期57卷 949-950页

作者： wu MingFu MENG FanXia Cardiovascular Science Center Albany Medical College Science China Press

Although the human adult heart was considered a terminally differentiated organ and incapable of renewal after injury or with aging for almost a century, recent studies have shown that the heart is capable of new cardiomyocyte formation and possesses varying degrees of regenerative potential throughout mammalian life. However, whether the cardiac stem cells （CSC） or the pre-existing cardiomyocytes con- tribute to the new cardiomyocyte formation remains con- troversial.

关键词：干细胞心脏心肌细胞终末分化哺乳动物 CSC

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