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Split-root systems applied to the study of the legume-rhizobial symbiosis: What have we learned?

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Journal of Integrative Plant Biology 2014年第12期56卷 1118-1124页

作者： Estíbaliz Larrainzar Erena Gil-Quintana cesar Arrese-Igor Esther M.González Daniel Marino Department of Environmental Sciences Public University of Navarra Pamplona E-31006 Spain Department of Plant Biology and Ecology University of the Basque Country UPV/EHU Bilbao E-48080 Spain Ikerbasque Basque Foundation for Science Bilbao E-48011 Spain

Split-root system（SRS） approaches allow the differential treatment of separate and independent root systems, while sharing a common aerial part. As such, SRS is a useful tool for the discrimination of systemic（shoot origin）versus local（root/nodule origin） regulation mechanisms. This type of approach is particularly useful when studying the complex regulatory mechanisms governing the symbiosis established between legumes and Rhizobium bacteria. The current work provides an overview of the main insights gained from the application of SRS approaches to understand how nodule number（nodulation autoregulation） and nitrogen fixation are controlled both under non-stressful conditions and in response to a variety of stresses. Nodule number appears to be mainly controlled at the systemic level through a signal which is produced by nodule/root tissue, translocated to the shoot, and transmitted back to the root system, involving shoot Leu-rich repeat receptor-like kinases. In contrast, both local and systemic mechanisms have been shown to operate for the regulation of nitrogenase activity in nodules. Under drought and heavy metal stress, the regulation is mostly local,whereas the application of exogenous nitrogen seems to exert a regulation of nitrogen fixation both at the local and systemic levels.

关键词： legume-rhizobia nitrogen fixation nodulation split-root system symbiosis

Strain-induced spatially indirect exciton recombination in zinc-blende/wurtzite CdS heterostructures

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Nano Research 2015年第9期8卷 3035-3044页

作者： Dehui Li Yang Liu Maria de la Mata cesar Magen Jordi Arbiol Yuanping Feng Qihua Xiong Division of Physics and Applied Physics School of Physical and Mathematical Sciences Nanyang Technological University Singapore 637371 Singapore Institut de Ciencia de Materials de Barcelona ICMAB-CSIC Campus de la UAB 08193 Bellaterra Catalonia Spain Laboratofio de Microscopias Avanzadas (LMA) Instituto de Nanociencia de Aragon (INA) ARAID and Departamento de Fisica de la Materia Condensada Universidad de Zaragoza 50018 Zaragoza Spain Institucio Catalana de Recerca i Estudis Avancats (ICREA) 08010 Barcelona Catalonia Spain Institut Catala de Nanociencia i Nanotecnologia (ICN2) Campus UAB 08193 Bellaterra Catalonia Spain Department of Physics National University of Singapore 2 Science Drive 3 Singapore 117542 Singapore Division of Microelectronics School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798Singapore

Strain engineering provides an effective mean of tuning the fundamental properties of semiconductors for electric and optoelectronic applications. Here we report on how the applied strain changes the emission properties of hetero- structures consisting of different crystalline phases in the same CdS nanobelts. The strained portion was found to produce an additional emission peak on the low-energy side that was blueshifted with increasing strain. Furthermore, the additional emission peak obeyed the Varshni equation with temperature and exhibited the band-filling effect at high excitation power. This new emission peak may be attributed to spatially indirect exciton recombination between different crystalline phases of CdS. First-principles calculations were performed based on the spatially indirect exciton recombination, and the calculated and experimental results agreed with one another. Strain proved to be capable of enhancing the anti-Stokes emission, suggesting that the efficiency of laser cooling may be improved by strain engineering.

关键词： strain,CdS nanobelts,photoluminescence,spatially indirect excitonrecombination,inter-crystalline phasetransition

Advance in the use of gold nanoparticles in the treatment of neurodegenerative diseases:new perspectives

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Neural Regeneration Research 2021年第12期16卷 2425-2426页

作者： Gustavo de Bem Silveira Alexandre Pastoris Muller Ricardo Andrez Machado-de-Ávila Paulo cesar Lock Silveira Laboratory of Experimental Pathophysiology Graduate Program in Health SciencesUniversidade do Extremo Sul CatarinenseCriciúmaSCBrazil Programa de Pós Graduação em Farmacologia UFSCFlorianópolisSCBrazil

Various conditions affecting nerve cells and the nervous system due to the loss of neurons and their connecting networks are described under the superordinate phrase“Neurodegenerative diseases”.Such diseases lead to disability due to gradual neuronal death in both the central nervous system and the peripheral nervous *** many of these diseases have unknown causes,sometimes these are due to medical conditions such as alcoholism,a tumor,or a stroke,or other causes which may include genetic mutations,toxins,chemicals,and viruses.

关键词： diseases degenerative treatment

N-Linked Glycosylation of the p24 Family Protein p24δ5 Modulates Retrograde Golgi-to-ER Transport of K/HDEL Ligands in Arabidopsis

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Molecular Plant 2017年第8期10卷 1095-1106页

作者： Noelia Pastor-Cantizano Maria Jesus Garcia-Murria cesar Bernat-Silvestre Maria Jesus Marcote Ismael Mingarro Fernando Aniento Departamento de Bioquimica y Biologia Molecular Universitat de Valencia 46100 Burjassot Spain Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED) Universitat de Valencia 46100 Burjassot Spain

The K/HDEL receptor ERD2 mediates the transport of soluble endoplasmic reticulum （ER）-resident proteins containing a C-terminal K/HDEL signal from the Golgi apparatus back to the ER via COPI （COat Protein I）-coated vesicles. Sorting of ERD2 within COPI vesicles is facilitated by p24 proteins. In Arabidop- sis, p24δ5 has been shown to interact directly with ERD2 via its luminal GOLD （GOLgi Dynamics） domain and with COPI proteins via its cytoplasmic C-terminal tail at the acidic pH of the Golgi apparatus. Several members of the p24 family in mammals and yeast have been shown to be glycosylated, but whether Arabidopsis p24 proteins are glycosylated and the role of the sugar moiety in p24 function remain unclear. Here, we show that Arabidopsis p24δ5 protein is N-glycosylated in its GOLD domain. Furthermore, we demonstrate that this post-translational modification is important for its coupled transport with p241β2 at the ER-Golgi interface, for its interaction with the K/HDEL receptor ERD2, and for retrograde transport of ERD2 and K/HDEL ligands from the Golgi apparatus back to the ER.

关键词： K/HDEL receptor COPI (COat Protein I) p24 proteins N-glycosylation

Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter

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Light(Science & Applications) 2015年第1期4卷 430-437页

作者： Andreu Llobera Joan Juvert Alfredo Gonzalez-Fernandez Bergoi Ibarlucea Ester Carregal-Romero Stephanus Buttgenbach cesar Fernandez-Sanchez Instituto de Microelectronica de Barcelona IMB-CNM(CSIC) Campus UAB08193 BellaterraSpain Institut fur Mikrotechnik Technische Universitat BraunschweigBraunschweig 38106Germany

A photonic lab on a chip(PhLOC),comprising a solid-state light emitter(SSLE)aligned with a biofunctionalized optofluidic multiple internal reflection(MIR)system,is *** SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane(PhTMOS)aqueous sol solution containing a fluorophore organic *** curing,the resulting xerogel solid structure retains the emitting properties of the fluorophore,which is evenly distributed in the xerogel *** studies demonstrate that after a total dose(at λ5365 nm)greater than 24 J cm^(-2),the xerogel emission decay is only 4.1%.To re-direct the emitted light,the SSLE includes two sets of air mirrors that surround the *** mapping of the SSLE demonstrates that alignment variations of 150 mm(between the SSLE and the external pumping light source)provide fluctuations in emitted light smaller than 5%.After this verification,the SSLE is monolithically implemented with a MIR,forming the *** performance is assessed by measuring quinolone yellow,obtaining a limit of detection(LOD)of(0.6060.01)***,the MIR is selectively biofunctionalized with horseradish peroxidase(HRP)for the detection of hydrogen peroxide(H_(2)O_(2))target analyte,obtaining a LOD of(0.760.1)μM for H_(2)O_(2),confirming,for the first time,that solid-state xerogel-based emitters can be massively implemented in biofunctionalized PhLOCs.

关键词： medical devices photonic lab on a chip polymer technology solid-state light emitters sol–gel

Dengue Climate Variability in Rio de Janeiro City with Cross-Wavelet Transform

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Journal of Environmental Protection 2022年第3期13卷 261-276页

作者： Suellen Araujo Franco dos Santos Hugo Abi Karam Augusto José Pereira Filho Jú lio cesar Barreto da Silva Jose Luis Flores Rojas Julio Migue Angeles Suazo Isela Leonor Vá squez Panduro cesar Arturo Sanchez Peñ a Departamento de Meteorologia Instituto de Geoci&ecirc ncias Universidade Federal do Rio de Janeiro (UFRJ) Rio de Janeiro Brasil Departamento de Ciê ncias Atmosfé ricas Instituto de Astronomia Geofísica e Ci&ecirc ncias Atmosfé ricas Universidade de S&atilde o Paulo (IAG-USP) S&atilde o Paulo Brasil Programa de Pós-Graduaç ã o em Ciê ncias Ambientais Universidade Estadual do Rio de Janeiro (UERJ) Rio de Janeiro Brasil Instituto Geofísico do Peru (IGP) Lima Per&uacute Facultad de Ingeniería Forestal y Ambiental. Universidad Nacional Autó noma de Tayacaja Daniel Herná ndez Morillo (UNAT) Pampas Per&uacute Universidad Tecnoló gicadel Perú Faculdad de Engenier&iacute a Industrial Lima Per&uacute Centro de Previsã o do Tempo e Estudos Climá ticos Cachoeira Paulista Brasil Programa de Pó s-Graduaç ã o em Computaç ã o Aplicada Instituto Nacional de Pesquisas Espaciais (INPE) S&atilde o José dos Campos S&atilde o Paulo SP Brasil

Dengue is one of the most prominent tropical epidemic diseases present in the Rio de Janeiro city and Southeast part of Brazil, due to the widespread conditions of occurrence of the dengue vector, the mosquito Aedesaegypti, such as high-temperature days interlaced with afternoon or nocturnal rainstorms in summer. This work has the objective of investigating the relationships between variabilities of the El Ni?o-South Oscillation (ENSO) and greater epidemics of dengue in Rio de Janeiro city. To accomplish this goal, the analysis and signal decomposition by cross-wavelet transform (WT) was applied to obtain the cross variability associated with variations of power and phase of both signals by characteristic periods and along with the time series. Data considered in the analysis are (the decimal logarithm of normalized value) of the monthly available notifications of dengue worsening, provided by the public health system of Brazil, and the Southern Oscillation Index (SOI) Ni?o 3.4 data, provided by the National Oceanic and Atmospheric Administration (NOAA), in the period 2000-2017. A maximum cross-wavelet power close to 0.45 was obtained for the representative period of 1 year and also to periods between 3 and 4 years, associated with the positive phase of the SOI index (i.e. , La Ni?a) or with a transition to the positive phase. The evolution of the combined variability of SOI and dengue can be expressed by progressive differences in phase along the time, eventually resulting in yielding phases (i.e., La Niña-Dengue epidemic).

关键词： Dengue Fever Cross-Wavelet Transform Analysis El Niño-Southern Oscillation Index

Cleft Palate Surgical Skills Training Using an Alternative Synthetic Bench Model

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Chinese Medical Journal 2016年第11期129卷 1382-1383页

作者： Andre Silveira Pinho cesar Augusto Raposo-Amaral Cassio Eduardo Raposo-Amaral Rafael Denadai Institute of Plastic and Craniofacial Surgery SOBRAPAR Hospital Caixa Postal: 6028 Campinas Sao Paulo Brazil

To the Editor： Although there have been numerous reports of difl＇erent synthetic simulators that can provide cleft palate training,none offers a completely ideal model.

关键词： SIMULATOR

Effect of increasing densities of Urochloa brizantha cv. Marandu on Eucalyptus urograndis initial development in silvopastoral system

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Journal of Forestry Research 2019年第2期30卷 537-543页

作者： Michel Anderson Almeida Colmanetti Allan Lopes Bacha Pedro Luis da Costa Aguiar Alves Rinaldo cesar de Paula University of Sao Paulo – USP/ESALQ Sao Paulo State University – UNESP/FCAV

In silvopasture system, the coexistence of eucalyptus seedlings with other species may result in growth reduction, especially during eucalyptus early ***, studies elucidating how forage species affect the eucalyptus growth can provide important information for their rational management aiming to obtain the maximum gain of the system. The aim of this work was to evaluate the effect of increasing densities of Urochloa brizantha *** in the early development of Eucalyptus urograndis. An experiment was conducted in 20 L pots, in an open and semi-controlled area, during 90 days after planting of eucalyptus. A completely randomized design with four replications was used, in a 6 9 7 factorial system, meaning six evaluation periods and seven densities of U. brizantha: 0(control), 22, 33, 44, 67, 89 and111 plants m^(-2). Fortnightly, eucalyptus height, stem diameter and chlorophyll fluorescence(Fv/Fm) were evaluated. At the end of experimental period, the net assimilation rate, stomatal conductance and transpiration rate of eucalyptus plants were determined, in addition to the dry matter of eucalyptus(leaves and stem) and U. brizantha (leaves). In coexistence with 111 plants m^(-2), eucalyptus had reduction of 63.9% on total dry matter and 72.7% on leaf area, compared to the control. From the density of22 plants m^(-2), U. brizantha negatively interfere significantly the growth of E. urograndis. Up to 8 plants m^(-2) there are no reductions greater than 5% in eucalyptus height and stem diameter.

关键词： Eucalyptus Palisade grass Competition Interference

Disease Biomarkers for Precision Medicine:Challenges and Future Opportunities

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Genomics, Proteomics & Bioinformatics 2017年第2期15卷 57-58页

作者： Edwin Wang William C.S.Cho S.C.cesar Wong Siqi Liu Center for Health Genomics and Informatics University of Calgary Cumming School of Medicine Department of Clinical Oncology Queen Elizabeth Hospital Department of Health Technology and Informatics Faculty of Health and Social Sciences Hong Kong Polytechnic University CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences

Most of the human diseases are complex diseases,which could be caused by many genetic *** means that for a given phenotype(i.e.,a complex disease),there are multiple potential genes which could be genomically or epigenetically changed(i.e.,mutations,copy number variations,epigenetic modifications,and so on).Therefore,it is understandable that different individuals who share the same phenotype/diseases may have different causal genes and thus,may have different drug *** example,mutated genes are rarely common between the cancer patients of the same cancer type[1];furthermore,for a given drug,only 10%-30% of the patients

关键词： data Disease Biomarkers for Precision Medicine:Challenges and Future Opportunities