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Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima

Synthetic and Systems Biotechnology 2022年第2期7卷 791-801页

作者： Paul Soudier Daniel Rodriguez Pinzon Tristan Reif-Trauttmansdorff Hassan Hijazi Maeva Cherriere catia goncalves pereira Doriane Blaise Maxime Pispisa Angelyne Saint-Julien William Hamlet Melissa Nguevo Eva Gomes Sophia Belkhelfa Anna Niarakis Manish Kushwaha Ioana Grigoras Evry Paris-Saclay 2020 iGEM team France Universite Paris-Saclay INRAEAgroParisTechMicalis Institute78352Jouy-en-JosasFrance Universite Paris-Saclay Univ EvryLaboratoire Europeen de Recherche pour la Polyarthrite Rhumatoïde-GenhotelEvryFrance Universite Paris-Saclay Univ EvryCNRSCEAGenomique MetaboliqueEvryFrance DNA Script Le Kremlin-BicetreFrance SYNMIKRO Center of Synthetic Microbiology MarburgGermany Universite Grenoble Alpes CEAInsermEDyP-BGEGrenobleFrance National Institute of Industrial Property(INPI) CourbevoieFrance

Nucleic acid sensing is a 3 decades old but still challenging area of application for different biological sub-domains,from pathogen detection to single cell transcriptomics *** many applications of nucleic acid detection and identification are mostly carried out by PCR techniques,sequencing,and their derivatives used at large ***,these methods’limitations on speed,cost,complexity and specificity have motivated the development of innovative detection methods among which nucleic acid biosensing technologies seem *** switches are a particular class of RNA sensing devices relying on a conformational switch of secondary structure induced by the pairing of the detected trigger RNA with a de novo designed synthetic sensing mRNA *** we describe a streamlined methodology enabling the development of such a sensor for the RNA-mediated detection of an endangered plant species in a cell-free reaction *** applied this methodology to help identify the rosewood Dalbergia maritima,a highly trafficked wood,whose protection is limited by the capacity of the authorities to distinguish protected logs from other unprotected but related *** streamlined pipeline presented in this work is a versatile framework enabling cheap and rapid development of new sensors for custom RNA detection.

关键词： Toehold switch Riboregulator Biosensor Rosewood

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Extracts of the seaweed Bifurcaria bifurcata display antifungal activity against human dermatophyte fungi

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Journal of Oceanology and Limnology 2019年第3期37卷 848-854页

作者： CARVALHO G.Loic SILVA Raquel goncalves J.Maria BATISTA T.Maria pereira Leonel Environmental and Biochemical Sciences Group the James Hutton InstituteDundee DD2 5DAScotlandUK Institute of Mechanical Process and Energy EngineeringSchool of Engineering and Physical SciencesHeriot Watt UniversityEdinburgh EH14 4ASScotlandUK MARE (Marine and Environmental Sciences Centre) Department of Life SciencesFaculty of Sciences and TechnologyUniversity of CoimbraCoimbra 3030-790Portugal CIEPQPF Department of Chemical EngineeringFaculty of Science and TechnologyUniversity of CoimbraCoimbra 3030-790Portugal

Bifurcaria bifurcata is a seaweed of the Order Fucales(Ochrophyta,Phaeophyceae)that can be found all year round along the Portuguese Atlantic *** it is considered edible in some countries,its biotechnological potential has not yet been assessed in detail and here we report its antimicrobial potential against human dermatophyte *** samples were harvested from Baleal Island(Peniche,Portugal)harvested in winter(BbPe);from Aguda Beach(Arcozelo,Vila Nova de Gaia,Portugal),harvested in autumn(BbAg)and a sample obtained by culturing the Aguda sample under laboratory conditions(BbLC).A broth macrodilution assay was applied to determine the MIC(minimum inhibitory concentration)and MLC(minimum lethal concentration)of the *** *** show that the BbPe MeOH extracts were the most effective and had higher antifungal activity against all the tested dermatophyte strains than the BbLC and BaAg extracts[Epidermophyton floccosum FF9(100–200μg/mL MIC;200μg/mL MLC),Microsporum canis FF1(400μg/mL MIC;≥400μg/mL MLC),Trichophyton mentagrophytes FF7(100μg/mL MIC;>800μg/mL MLC),*** CECT 2908(800μg/mL MIC;≥800μg/mL MLC),*** *** CECT 2958(800μg/mL MIC;≥800μg/mL MLC),*** CECT 2794(200μg/mL MIC;≥400μg/mL MLC)].In fact,only *** FF9 was sensitive to BbAg and BbLC MeOH *** our knowledge,this is the first report of antifungal activity of *** against human dermatophyte fungi.

关键词： seaweed Bifurcaria bifurcata bioactivity antifungal dermatophytes minimum inhibitory concentration minimum lethal concentration

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In vitro toxicity of particulate matter emissions from residential pellet combustion

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Journal of Environmental Sciences 2022年第5期34卷 215-226页

作者： Estela D.Vicente Daniela Figueiredo catia goncalves Isabel Lopes Helena Oliveira Nora Kovats Teresa Pinheiro Celia A.Alves Department of Environment and Planning and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Department of Biology and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Centre of Natural Environmental Sciences University of PannoniaEgyetem str.108200 VeszpremHungary Instituto de Bioengenharia e Biociencias Departamento de Engenharia e Ciencias NuclearesInstituto Superior TecnicoUniversidade de LisboaAv.Rovisco Pais 11049-001 LisboaPortugal

Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell *** was evaluated through the Vibrio fischeri bioluminescence inhibition ***,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells *** Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 *** obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were *** samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was ***10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower ***-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified *** activity was not detected in both *** murium *** study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM *** raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.

关键词： Ecotoxicity Cytotoxicity Mutagenicity Fuel quality Pellet combustion PM10 emissions

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Carcass image segmentation using CNN-based methods

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Information Processing in Agriculture 2021年第4期8卷 560-572页

作者： Diogo Nunes goncalves Vanessa Aparecida de Moares Weber Julia Gindri Bragato Pistori Rodrigo da Costa Gomes Anderson Vicoso de Araujo Marcelo Fontes pereira Wesley Nunes goncalves Hemerson Pistori Federal University of Mato Grosso do Sul Av.Costa e Silvas/nBairro UniversitarioCampo GrandeMS 79070-900Brazil INOVISAO Dom Bosco Catholic UniversityAvenida Tamandare6000CP 100Jardim SeminarioCampo GrandeMS 79117-900Brazil EMBRAPA Gado de Corte Av.Radio Maia830Vila PopularCampo GrandeMS 79106-550Brazil State University of Mato Grosso do Sul Av.Dom Antonio Barbosa4155Vila Santo AmaroCampo GrandeMS 79115-898Brazil

Carcass grading can be used as an important metric to determine meat ***,carcass grading is usually performed by a specialist,making it a subjective and errorprone *** increase the accuracy of such task,image-based systems have been proposed in the *** of the most important parts of an image-based system is the image segmentation,which aims to identify the regions of the carcass in the *** this paper,we propose the use of two recent image segmentation methods called Superpixel+CNN(Convolutional Neural Network)and *** evaluate both methods,we have also built a dataset of carcass images and their *** of approximately 96%of pixel accuracy show the robustness of the methods in carcass image *** novelty of this work is the proposal and comparison of recent deep learning methods that use CNN and superpixels in carcass *** this way,the methods can be used in carcass grading systems to increase the accuracy of the grading task.

关键词： Carcass grading Image segmentation Convolutional neural networks Superpixels

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First Serological Evidence on Endemicity of HEV Infection in Wild Boar(Sus scrofa) Populations from Portugal

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Virologica Sinica 2018年第2期33卷 197-200页

作者： Daniel goncalves Joao pereira-Vaz Vitor Duque Victor Bandeira Carlos Fonseca Ana Donato Cristina Luxo Ana Miguel Matos Laboratory of Virology Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Laboratory of Molecular Biology Clinical Pathology UnitHospital and University Centre of Coimbra3000-602 Coimbra Portugal Infectious Diseases Department Hospital and University Centre of Coimbra 3000-075 Coimbra Portugal Biology Department and CESAM University of Aveiro3810-193 Aveiro Portugal Clinical Laboratory Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Research Centre on Chemical Processes Engineering and Forest Products (CIEPQF) Faculty of Pharmacy University of Coimbra 3030-790 Coimbra Portugal

Increasing number of hepatitis E virus（HEV）infection has been described in industrialized countries,with infection occurring as a sporadic disease,usually associated with travel to endemic regions or small outbreaks... 详细信息

关键词：感染 HEV 血清学葡萄牙野猪证据特性人口

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Building Envelope Rehabilitation Costs： Determination and Variability

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Journal of Civil Engineering and Architecture 2012年第3期6卷 300-307页

作者： Carina Isabel pereira de Sousa Joao Carlos goncalves Lanzinha Civil Engineering and Architecture Department Faculty of Engineering University of Beira Interior Covilh6 Portugal

In recent decades, there has been a great deal of investment in Portugal in the construction of new buildings to the detriment of the rehabilitation of existing ones. As a result, the historic centres of towns and cities are deteriorating, while their suburbs are spreading. One of the main problems affecting the rehabilitation of residential buildings is that it is extremely difficult to accurately estimate the costs involved. Although there are established methods （and technical documents available） to aid the costing of new buildings, there is a lack of official information about the costs of rehabilitation work, particularly as regards the external envelope of buildings. This article demonstrates how to determine rehabilitation costs （with particular emphasis on the envelope of residential buildings） by gathering information from specialized companies, assessing price variability and consulting databases of rehabilitation costs from other countries. It also presents some examples of costing for particular rehabilitation jobs.

关键词： Rehabilitation building envelope costs.

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Whole body vibration and auriculotherapy improve handgrip strength in individuals with knee osteoarthritis

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Journal of Traditional Chinese Medicine 2019年第5期39卷 707-715页

作者： Eloa Moreira-Marconi Carla F Dionello Danielle S Morel Danubia C Sa-Caputo Cintia R Sousa-goncalves Laisa L Paineiras-Domingos Ygor Teixeira-Silva Mario Jose dos Santos pereira Mario Bernardo-Filho Postgraduate Program in Clinical and Experimental Physiopathology University of the State of Rio de JaneiroRio de JaneiroRJBrazil Department of Biophysics and Biometrics Laboratory of Mechanical Vibrations and Integrative Practices-LAVIMPIInstitute of Biology Roberto Alcantara GomesRio de Janeiro State UniversityRio de JaneiroRJBrazil Postgraduate Program in Medical Sciences Rio de Janeiro State UniversityRio de JaneiroRJBrazil Danubia C Sa-Caputo Faculdade Bezerra de AraujoRio de JaneiroBrazilCentro Universitario Serra dos OrgaosTeresopolisRio de JaneiroBrasil Universidade do Estado do Rio de Janeiro Rio de JaneiroRJBrazil-Department of Biophysics and BiometricsInstitute of Biology Roberto Alcantara Gomes-Department of Physiological Sciences

OBJECTIVE: To verify the action of non-pharmacological interventions, whole body vibration exercise (WBVE) and auriculotherapy (AT) on the management of knee osteoarthritis (KOA) analyzing the handgrip strength (HS). METHODS: One hundred twelve participants with KOA were allocated in (a) WBVE group with peak-to-peak displacement of 2.5 to 7.5 mm, frequency from 5 up to 14 Hz, acceleration peak from 0.12 up to 2.95 g (2 d/weekly for 5 weeks),(b) AT group, points of both ears (Kidney, Knee-correspondent point and Shenmen) were stimulated with seeds,(c) WBVE + AT group and (d) respective control groups. HS was assessed in all the participants, in acute and cumulative responses. RESULTS: The intervention with WBVE alone and combined with AT improved (P < 0.05), in a cumulative response, the HS. CONCLUSION: WBVE alone or combined with AT might promote biological effects that interfere with the HS in individuals with KOA.

关键词： Osteoarthritis,knee Exercise therapy Physical and rehabilitation medicine Auriculotherapy Muscle strength dynamometer

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Effect of Massage Therapy in Cancer Patients

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International Journal of Clinical Medicine 2017年第2期8卷 111-121页

作者： Marcia Alves Maria Helena de Agrela goncalves Jardim Barbara pereira Gomes Biomedical Scientific Institute Abel Salazar PortoPortugal Hospital Dr.Nelio Mendonca FunchalPortugal Madeira University FunchalPortugal School of Nursing of the Porto PortoPortugal

The increase in longevity and incidence of chronic diseases reveals an increased importance in terms of public health. The oncologic illness is a debilitating and progressive pathology with need for prevention and symptomatic relief. In order to find the answer to the question: “What is the effect of massage therapy in cancer patients?” we have reviewed the empirical literature indexed in databases online, finding only 21 articles published between 1990 and 2015. It was possible to verify some of the effects of massage therapy, particularly in relieving pain, decreased anxiety, depression and nausea and increased well-being. However, it was not found the effect of this intervention on the relief of suffering and the quality of life of patients. With the heterogeneity of methodologies, studies suggest the development of more homogeneous research, materials and methods to assess the effects of massage therapy in cancer patients.

关键词： Patients Oncology Massage

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Antiglycation and antioxidant activities of the crude extract and saponin fraction of Tribulus terrestris before and after microcapsule release

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Journal of Integrative Medicine 2022年第2期20卷 153-162页

作者： Célia Cristina Malaguti Figueiredo Amanda da Costa Gomes Filipe Oliveira Granero Joao Luiz Bronzel Junior Luciana pereira Silva Valdecir Farias Ximenes Regildo Márcio goncalves da Silva Institute of Chemistry São Paulo State UniversityAraraquaraSão Paulo 14800-060Brazil Educational Foundation of the City of Assis AssisSão Paulo 19807-130Brazil Department of Chemistry School of SciencesSão Paulo State UniversityBauruSão Paulo 17033-360Brazil Department of Biotechnology School of SciencesHumanities and LanguagesSão Paulo State UniversityAssisSão Paulo 19806-900Brazil

Objective:The present study investigated antiglycation and antioxidant activities of crude dry extract and saponin fraction of Tribulus *** also developed a method of microencapsulation and evaluated antiglycation and antioxidant activities of crude dry extract and saponin fraction before and after microcapsule ***:Antiglycation activity was determined by relative electrophoretic mobility(REM),free amino groups and inhibition of advanced glycation end-product(AGE)*** activity was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH),ferric ion-reducing antioxidant power(FRAP),nitric oxide(NO)and thiobarbituric acid reactive species(TBARS)*** were prepared using maltodextrin as wall material and freeze-drying as encapsulation *** characterization of microcapsules was evaluated by scanning electron microscopy,and encapsulation efficiency and microcapsule release were determined by total saponins *** and antioxidant assays were performed using crude dry extract and saponin fraction of *** before and after ***:Saponin fraction showed an increase of 32.8%total ***-performance liquid chromatography-mass spectrometry analysis showed the presence of saponins in the obtained *** evaluation by REM demonstrated that samples before and after release presented antiglycation activity similar to bovine serum albumin treated with ***,samples inhibited AGE formation,highlighting treatment with saponin fraction after release(89.89%).Antioxidant tests demonstrated antioxidant activity of the *** dry extract before encapsulation presented the highest activities in DPPH(92.00%)and TBARS(32.49%)*** fraction before encapsulation in FRAP test(499μmol Trolox equivalent per gram of dry sample)and NO test(15.13μmol nitrite formed per gram of extract)presented the highest ***:This study presen

关键词： Glycation Microencapsulation Oxidative stress Saponins Tribulus terrestris

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Analytical solution of modified point kinetics equations for linear reactivity variation in subcritical nuclear reactors adopting an incomplete gamma function approximation

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Natural Science 2012年第11期4卷 919-923页

作者： André Luiz pereira Rebello Junior Aquilino Senra Martinez Alessandro da Cruz goncalves Nuclear Engineering Department Federal University of Rio de Janeiro Rio de Janeiro Brazil

The present work aims to achieve a fast and accurate analytical solution of the point kinetics equations applied to subcritical reactors such as ADS (Accelerator-Driven System), assuming a linear reactivity and external source variation. It was used a new set of point kinetics equations for subcritical systems based on the model proposed by Gandini & Salvatores. In this work it was employed the integrating factor method. The analytical solution for the case of interest was obtained by using only an approximation which consists of disregarding the term of the second derivative for neutron density in relation to time when compared with the other terms of the equation. And also, it is proposed an approximation for the upper incomplete gamma function found in the solution in order to make the computational processing faster. In addition, for purposes of validation and comparison a numerical solution was obtained by the finite differences method. Finally, it can be concluded that the obtained solution is accurate and has fast numerical processing time, especially when compared with the results of numerical solution by finite difference. One can also observe that the gamma approximation used achieve a high accuracy for the usual parameters. Thus we got satisfactory results when the solution is applied to practical situations, such as a reactor startup.

关键词： Accelerator-Driven System Subcritical Reactors Point Kinetics Equations Incomplete Gamma Functions

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