Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima

作     者：Paul Soudier Daniel Rodriguez Pinzon Tristan Reif-Trauttmansdorff Hassan Hijazi Maeva Cherriere Catia Goncalves Pereira Doriane Blaise Maxime Pispisa Angelyne Saint-Julien William Hamlet Melissa Nguevo Eva Gomes Sophia Belkhelfa Anna Niarakis Manish Kushwaha Ioana Grigoras 

作者机构：Evry Paris-Saclay 2020 iGEM teamFrance Universite Paris-SaclayINRAEAgroParisTechMicalis Institute78352Jouy-en-JosasFrance Universite Paris-SaclayUniv EvryLaboratoire Europeen de Recherche pour la Polyarthrite Rhumatoïde-GenhotelEvryFrance Universite Paris-SaclayUniv EvryCNRSCEAGenomique MetaboliqueEvryFrance DNA ScriptLe Kremlin-BicetreFrance SYNMIKRO Center of Synthetic MicrobiologyMarburgGermany Universite Grenoble AlpesCEAInsermEDyP-BGEGrenobleFrance National Institute of Industrial Property(INPI)CourbevoieFrance 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2022年第7卷第2期

页      面：791-801页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：the Evry Paris-Saclay 2020 iGEM team's project that was generously funded by our sponsors: Genopole, University of Evry-val-d'Essonne, University of Paris-Saclay and Investissements d'avenir support by the ANR SynBioDiag grant MK from INRAE's MICA department, Universite Paris-Saclay, Ile-de-France (IdF) region's DIM-RFSI, and ANR DREAMY 

主　　题：Toehold switch Riboregulator Biosensor Rosewood 

摘      要：Nucleic acid sensing is a 3 decades old but still challenging area of application for different biological sub-domains,from pathogen detection to single cell transcriptomics *** many applications of nucleic acid detection and identification are mostly carried out by PCR techniques,sequencing,and their derivatives used at large ***,these methods’limitations on speed,cost,complexity and specificity have motivated the development of innovative detection methods among which nucleic acid biosensing technologies seem *** switches are a particular class of RNA sensing devices relying on a conformational switch of secondary structure induced by the pairing of the detected trigger RNA with a de novo designed synthetic sensing mRNA *** we describe a streamlined methodology enabling the development of such a sensor for the RNA-mediated detection of an endangered plant species in a cell-free reaction *** applied this methodology to help identify the rosewood Dalbergia maritima,a highly trafficked wood,whose protection is limited by the capacity of the authorities to distinguish protected logs from other unprotected but related *** streamlined pipeline presented in this work is a versatile framework enabling cheap and rapid development of new sensors for custom RNA detection.