检索结果-南通市图书馆

In vitro toxicity of particulate matter emissions from residential pellet combustion

Journal of Environmental Sciences 2022年第5期34卷 215-226页

作者： Estela D.Vicente Daniela Figueiredo catia goncalves Isabel Lopes Helena Oliveira Nora Kovats Teresa Pinheiro Celia A.Alves Department of Environment and Planning and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Department of Biology and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Centre of Natural Environmental Sciences University of PannoniaEgyetem str.108200 VeszpremHungary Instituto de Bioengenharia e Biociencias Departamento de Engenharia e Ciencias NuclearesInstituto Superior TecnicoUniversidade de LisboaAv.Rovisco Pais 11049-001 LisboaPortugal

Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell *** was evaluated through the Vibrio fischeri bioluminescence inhibition ***,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells *** Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 *** obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were *** samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was ***10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower ***-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified *** activity was not detected in both *** murium *** study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM *** raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.

关键词： Ecotoxicity Cytotoxicity Mutagenicity Fuel quality Pellet combustion PM10 emissions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima

引用

Synthetic and Systems Biotechnology 2022年第2期7卷 791-801页

作者： Paul Soudier Daniel Rodriguez Pinzon Tristan Reif-Trauttmansdorff Hassan Hijazi Maeva Cherriere catia goncalves Pereira Doriane Blaise Maxime Pispisa Angelyne Saint-Julien William Hamlet Melissa Nguevo Eva Gomes Sophia Belkhelfa Anna Niarakis Manish Kushwaha Ioana Grigoras Evry Paris-Saclay 2020 iGEM team France Universite Paris-Saclay INRAEAgroParisTechMicalis Institute78352Jouy-en-JosasFrance Universite Paris-Saclay Univ EvryLaboratoire Europeen de Recherche pour la Polyarthrite Rhumatoïde-GenhotelEvryFrance Universite Paris-Saclay Univ EvryCNRSCEAGenomique MetaboliqueEvryFrance DNA Script Le Kremlin-BicetreFrance SYNMIKRO Center of Synthetic Microbiology MarburgGermany Universite Grenoble Alpes CEAInsermEDyP-BGEGrenobleFrance National Institute of Industrial Property(INPI) CourbevoieFrance

Nucleic acid sensing is a 3 decades old but still challenging area of application for different biological sub-domains,from pathogen detection to single cell transcriptomics *** many applications of nucleic acid detection and identification are mostly carried out by PCR techniques,sequencing,and their derivatives used at large ***,these methods’limitations on speed,cost,complexity and specificity have motivated the development of innovative detection methods among which nucleic acid biosensing technologies seem *** switches are a particular class of RNA sensing devices relying on a conformational switch of secondary structure induced by the pairing of the detected trigger RNA with a de novo designed synthetic sensing mRNA *** we describe a streamlined methodology enabling the development of such a sensor for the RNA-mediated detection of an endangered plant species in a cell-free reaction *** applied this methodology to help identify the rosewood Dalbergia maritima,a highly trafficked wood,whose protection is limited by the capacity of the authorities to distinguish protected logs from other unprotected but related *** streamlined pipeline presented in this work is a versatile framework enabling cheap and rapid development of new sensors for custom RNA detection.

关键词： Toehold switch Riboregulator Biosensor Rosewood

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：