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Tyrosinase Biosensor Used for the Determination of Catechin Derivatives in Tea: Correlation with HPLC/DAD Method

作者： (英)carole·Sharman等原

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Food and Nutrition Sciences 2013年第1期4卷 108-118页

作者： Saloua Nadifiyine carole Calas-Blanchard Aziz Amine Jean-Louis Marty Institut de Modélisation et d’Analyse en Géo-Environnement et Santé Univ. Perpignan Via Domitia Perpignan France Institut de Modélisation et d’Analyse en Géo-Environnement et Santé Univ. Perpignan Via Domitia Perpignan France&Laboratoire d’Analyses Chimiques et Biocapteurs Faculté des Sciences et Techniques Université Hassan II Mohammedia Morocco. Laboratoire d’Analyses Chimiques et Biocapteurs Faculté des Sciences et Techniques Université Hassan II Mohammedia Morocco.

Tea, one of the most popular consumed worldwide beverages, is known to be rich in polyphenols, more particularly in catechins. An amperometric biosensor based on tyrosinase was developed in order to monitor the phenolic content in black and green teas. The enzyme was immobilised by coreticulation with glutaraldehyde on carbon screen-printed electrodes (CSPE). Initially, the performances of the biosensor were evaluated referring to catechol as a model substrate. This analytical tool exhibits a high sensitivity (217 nA/μM), low limit of detection (LOD) = 0.03 μM, good intra-electrode and inter-electrode reproducibilities with RSD lower than 3% (n = 5 injections) and RSD = 8.14% (n = 12 sensors) respectively. The storage stability was also studied;the biosensor retained successively 85% and 70% of its initial response after 34 and 53 days. Subsequently, several catechin derivatives frequently found in teas were tested and classified relatively to their sensitivities. For tea samples, the results obtained with the biosensors were compared to high performance liquid chromatography (HPLC) analysis. A good correlation between the two methods was obtained. The calculated recovery was between 90% and 96%, proving that the proposed tyrosinase biosensor can be an alternative analytical tool for global determination of catechin derivatives in tea.

关键词： Tea Polyphenol Catechin Tyrosinase Amperometric Biosensor HPLC

Exploitation of Electromagnetic Models for Sea Wind Speed Estimation from C-Band Sentinel-1 Images

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Journal of Electromagnetic Analysis and Applications 2016年第3期8卷 42-55页

作者： Tran Vu La Ali Khenchaf Fabrice Comblet carole Nahum Helmi Ghanmi Lab-STICC UMR CNRS 6285 ENSTA Bretagne Brest France French General Directorate for Armament (DGA) Paris France

Among the different available wind sources, i.e. in situ measurements, numeric weather models, the retrieval of wind speed from Synthetic Aperture Radar (SAR) data is one of the most widely used methods, since it can give high wind resolution cells. For this purpose, one can find two principal approaches: via electromagnetic (EM) models and empirical (EP) models. In both approaches, the Geophysical Model Functions (GMFs) are used to describe the relation of radar scattering, wind speed, and the geometry of observations. By knowing radar scattering and geometric parameters, it is possible to invert the GMFs to retrieve wind speed. It is very interesting to compare wind speed estimated by the EM models, general descriptions of radar scattering from sea surface, to the one estimated by the EP models, specific descriptions for the inverse problem. Based on the comparisons, some ideas are proposed to improve the performance of the EM models for wind speed retrieval.

关键词： C-Band Synthetic Aperture Radar (SAR) Electromagnetic (EM) Scattering Models Radar Cross-Section (RCS) Remote Sensing Small Perturbation Model (SPM) Sea Surface Wind

Genetic identification and reiterated captures suggest that the Astyanax mexicanus El Pachón cavefish population is closed and declining

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Zoological Research 2023年第4期44卷 701-711页

作者： Laurent Legendre Julie Rode Isabelle Germon Marie Pavie Carla Quiviger Maxime Policarpo Julien Leclercq Stéphane Père Julien Fumey carole Hyacinthe Patricia Ornelas-García Luis Espinasa Sylvie Rétaux Didier Casane UniversitéParis-Saclay CNRSIRDUMRÉvolutionGénomesComportement etÉcologieGif-sur-Yvette 91190France Objectif Sciences International NGO OSI-Panthera ProgramGeneva 99140Switzerland Institut de Neuroscience Paris-Saclay UniversitéParis-Saclay and CNRSSaclay 91400France Institut Pasteur UniversitéParis CitéCNRS UMR 2000Microbial Paleogenomics UnitParis 75015France Institut Pasteur UniversitéParis CitéBioinformatics and Biostatistics HubParis 75015France Department of Genetics Harvard Medical SchoolBlavatnik InstituteBoston MA 02115USA Colección Nacional de Peces Departamento de ZoologíaInstituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico DF CP 04510México School of Science Marist CollegePoughkeepsieNew York 12601USA UniversitéParis Cité UFR Sciences du VivantParis 75013France Zoological Institute Department of Environmental SciencesUniversity of BaselBaselSwitzerland

The sizes of Astyanax mexicanus blind cavefish populations of North-East Mexico are demographic parameters of great importance for investigating a variety of ecological,evolutionary,and conservation ***,few estimates have been *** these mobile animals living in an environment difficult to explore as a whole,methods based on capture-mark-recapture are appropriate,but their feasibility and interpretation of results depend on several assumptions that must be carefully ***,we provide evidence that minimally invasive genetic identification from captures at different time intervals(three days and three years)can give insights into cavefish population size dynamics as well as other important demographic parameters of *** also provide tools to calibrate sampling and genotyping efforts necessary to reach a given level of *** results suggest that the El Pachón cave population is currently very small,of an order of magnitude of a few hundreds of individuals,and is distributed in a relatively isolated *** probable decline in population size in the El Pachón cave since the last census in 1971 raises serious conservation issues.

关键词： Cavefish Population size Conservation Swabbing Genetic identification

Structural Models of the Human Chemokine Receptor CCR5

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Structural Models of the Human Chemokine Receptor CCR5

Operational continental-scale land cover mapping of Australia using the Open Data Cube

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Karthik Arumugam carole Devaux Jean Claude Schmit Retrovirology Laboratory Centre de Recherche Public de la SanteLuxembourgLuxembourg

G-protein-coupled receptors(GPCRs) constitute a large protein family of integral membrane receptors and make up roughly 3%of genes in the entire human *** function is to transduce a wide variety of ***,structural info... 详细信息

关键词： GPCR CCR5 Molecular Dynamics Lipid membrane 3D-structure

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International Journal of Digital Earth 2022年第1期15卷 1715-1737页

作者： Christopher J.Owers Richard M.Lucas Daniel Clewley Belle Tissott Sean M.T.Chua Gabrielle Hunt Norman Mueller carole Planque Suvarna M.Punalekar Pete Bunting Peter Tan Graciela Metternicht School of Environmental and Life Sciences University of NewcastleCallaghanAustralia Department of Geography and Earth Sciences Aberystwyth UniversityAberystwythUK Commonwealth Scientific and Industrial Research Organisation Land and WaterCanberraAustralia Centre for Geospatial Applications Plymouth Marine LaboratoryPlymouthUK Geoscience Australia SymonstonAustralia Earth and Sustainability Science Research Centre University of New South WalesSydneyAustralia School of Science Western Sydney UniversityRichmondAustralia

To comprehensively support national and international initiatives for sustainable development,land cover products need to be reliably and routinely generated within operational *** with consistent semantics and taxonomies,ensuring confidence in mapping land cover for multiple time periods,facilitates informed decision-making at scales appropriate to multiple policy *** United Nations Food and Agriculture Organisation(FAO)Land Cover Classification System(LCCS)provides a taxonomy that comparable at different scales,level of detail and geographic *** Open Data Cube(ODC)initiative offers a framework for operational continental-scale land cover mapping using analysis-ready Earth Observation *** study utilised the FAO LCCS framework and the Landsat sensor data through Digital Earth Australia(DEA;Australia’s ODC instance)to generate consistent and continent-wide land cover mapping(DEA Land Cover)of the Australian *** Land Cover provides annual maps from 1988 to 2020 at 25 m *** maps were validated with∼12,000 independent validation points,giving an overall map accuracy of 80%.DEA Land Cover provides Australia with a nationally consistent picture of land cover,with an open-source software package using readily available global coverage data and demonstrates a pathway of adoption for national implementations across the world.

关键词： DEA land cover digital earth Australia FAO LCCS landsat sustainable development goals

Heparan-mimetics:Potential agents of tissue regeneration for bone and periodontal therapies

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Medicine in Novel Technology and Devices 2021年第3期11卷 1-10页

作者： Maroun Bou Karam Joe El Khoury carole Chakar Sylvie Changotade Didier Lutomski Nada Naaman Gaston Godeau Abdellatif Elm’selmi Ronald Younes Karim Senni Craniofacial Research Laboratory Faculty of Dental MedicineSaint Joseph University of BeirutLebanon Department of Periodontology Faculty of Dental MedicineSaint Joseph University of BeirutLebanon Universite Sorbonne Paris Nord Unite de Recherche Biomateriaux Innovants et InterfacesURB2IUR 4462F-93000BobignyFrance Universite de Paris F-92049MontrougeFrance EBInnov School of Industrial Biology-EBICergyFrance Department of Oral Surgery Faculty of Dental MedicineSaint Joseph University of BeirutLebanon

Heparan sulfate glycosaminoglycans are key players of tissue repair and can be regarded as useful compounds for regenerative ***,their therapeutic uses face many technical,industrial,and regulatory hurdles due to their animal ***,some non-animal sulfated polysaccharides mimic heparan sulfate properties and offer interesting solutions to replace *** them,dextran derivatives,seaweed polysaccharides,or marine bacterial polysaccharides are the best known and have demonstrated their pro-regenerative capabilities by promoting both extracellular matrix structuring and angiogenesis and limiting degenerative processes such as inflammatory cell migration or tissue *** polysaccharides have also shown their ability to specifically promote osteoblastic differentiation and bone wound ***,recent works shows that heparan-mimetics can be used as an additive to improve the cytocompatibility of bone substitutes commonly used in periodontal *** use of these polysaccharides can be regarded as a clever approach to improve the biointegration of bone substitutes.

关键词： Bone biomaterials Periodontal surgery Heparan-mimetics Marine biotechnology Dextran derivatives Bacterial exopolysaccharides Fucoidans

Investigation of cardiomyopathy using cardiac magnetic resonance imaging part 1:Common phenotypes

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World Journal of Cardiology 2012年第4期4卷 103-111页

作者： Shaunagh McDermott Ailbhe C O’Neill carole A Ridge Jonathan D Dodd Cardiac CT and MRI Program St.Vincent’s University HospitalElm ParkDublin 4Ireland

Cardiac magnetic resonance imaging(CMRI) has emerged as a useful tertiary imaging tool in the investigation of patients suspected of many different types of cardiomyopathies,CMRI sequences are now of a sufficiently robust quality to enable high spatial and temporal resolution image *** has led to CMRI becoming an effective non-invasive imaging gold standard for many *** this 2-part review,we outline the typical sequences used to image cardiomyopathy,and present the imaging spectrum of *** 1 focuses on the current classification of cardiomyopathy,basic CMRI sequences used in evaluating cardiomyopathy and the imaging spectrum of common phenotypes.

关键词： Cardiac magnetic resonance imaging Cardiomyopathies

Ship Collision under French Maritime Law:Comparative Study with Chinese Law

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Ship Collision under French Maritime Law:Comparative Study w...

作者： BEBEY YONDO KINGUE AKWA carole EUGENIE 大连海事大学

学位级别：硕士

在海事请求中,30%的案件为船舶碰撞案件。虽然近年来随着现代船舶技术的发展,船舶碰撞的数量有所减少,但蒸汽导航和钢体外壳船舶的出现增加了碰撞损伤的严重程度。船舶碰撞之后会对海洋和人类生活以及环境带来无法估量的有害影响。与以... 详细信息

在海事请求中,30%的案件为船舶碰撞案件。虽然近年来随着现代船舶技术的发展,船舶碰撞的数量有所减少,但蒸汽导航和钢体外壳船舶的出现增加了碰撞损伤的严重程度。船舶碰撞之后会对海洋和人类生活以及环境带来无法估量的有害影响。与以前相比,现代航运设备能够帮助我们更准确地找到船舶碰撞的责任人。由于船舶潜在的沉没危险以及碰撞所造成的巨大破坏,船舶碰撞无疑是海员所面临的最可怕的灾难之一。船舶碰撞通常源于人为失误,因此,人为因素是找到在瞭望或者防碰撞演习中导致失误的原因时所必须考虑的因素,这样就可以找到弥补这些失误的方法了。船舶碰撞的另一点特殊之处在于,在海商法下,它们受益于特定的制度,包括在法国法律中,其法律制度也是以过失和疏忽为基础的少数制度之一。然而,船舶碰撞法律制度是一个古老的法律制度,需要适应现代海上运输方式的演变。这种特殊性还会通过专门的司法管辖转化:海事商事法院以及明确的商船船员惩戒条例。本文的写作目的是为了明确法国法下海上碰撞概念的法律地位、船舶碰撞责任确立及分担所要考虑的法律依据、当前国际公约中对于船舶碰撞这一问题的相关规定及如何明确损害赔偿责任分摊问题的规定所产生的众多影响。同时,本文意在借助船舶碰撞案件将法国与中国法律法规进行对比,找到其中的关联性并进行分析。本文从国家法律和相关国际公约的角度,对两国的碰撞制度和损害赔偿制度进行了比较,以期对受害方进行赔偿。本文共分为五章,作者从不同章节的论述中得出了最终结论。第一章"导论",表明了本文的研究背景、研究方法以及写作目的。第二章"碰撞案件的管辖权和法律适用",阐述了船舶碰撞诉讼中的管辖权,以及本文涉及的基本概念,如船舶碰撞的定义、特征和构成要件。同时区别了法国船舶碰撞法律的国际渊源和适用于船舶碰撞案件的国内法的不同之处。第三章"碰撞责任制度"。本章借鉴国际公约对法国海商法关于赔偿责任制度的影响,重点分析和比较了法国和中国对船舶碰撞损害赔偿责任主体的立法。第四章"碰撞损害"。本章指出了法国可恢复补偿的损害赔偿的不同种类及其评估原则。第五章"结语:中国和法国法律对比"。总结了由船舶碰撞产生的不同之处,法国与中国法律关于船舶碰撞规定的相同点和不同点。

关键词：民事责任船舶碰撞船舶碰撞法规损害赔偿

Encapsulation-based technologies for bioactive compounds and their application in the food industry:A roadmap for food-derived functional and health-promoting ingredients

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Food Bioscience 2022年第6期50卷 1-16页

作者： Muhammad H.Alu'datt Mohammad Alrosan Sana Gammoh carole C.Tranchant Mohammad N.Alhamad Taha Rababah Roa'a Zghoul Haya Alzoubi Salsabeel Ghatasheh Kawther Ghozlan Thuan-Chew Tan Department of Nutrition and Food Technology Faculty of AgricultureJordan University of Science and TechnologyP.O.Box 3030Irbid22110Jordan Food Technology Division School of Industrial TechnologyUniversiti Sains Malaysia11800 USMPenangMalaysia School of Food Science Nutrition and Family StudiesFaculty of Health Sciences and Community ServicesUniversité de MonctonMonctonNew BrunswickE1A 3E9Canada Department of Natural Resources and Environment Faculty of AgricultureJordan University of Science and TechnologyP.O.Box 3030Irbid22110Jordan School of Agriculture and Food Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkville3010VICAustralia

Some of the most commonly used agri-food products worldwide are vegetable and fruit-based products that are rich in bioactive compounds such as vitamins,polyphenols,and other ***,many of these molecules are sensitive and susceptible to oxidation and degradation during food processing,particularly when exposed to *** review examines the effect of encapsulation on bioactive compounds and how encapsulation technologies can be leveraged in the food industry to protect and optimize the functional properties of naturally occurring food *** can also assist with the creation of desirable sensory attributes(e.g.,aroma,texture,color,and taste),thus playing a major role in the design and development of novel foods and *** is one of the few technologies experiencing continued growth due to its unique potentialities,high versatility and extensive range of *** types of food grade encapsulating materials,also known as wall,coating,shell or carrier,can be employed,including proteins,polysaccharides(e.g.,gums),other biopolymers,and *** owes its success to its proven record as an effective process for preserving the encapsulated bioactives from the surrounding conditions,while assisting with optimized delivery and controlled release of the transported active *** the multiple variations and applications of encapsulation,micro-and nanoencapsulation of food-derived bioactives are the main focus of this review,which discusses the underlying principles,technological developments,as well as current and foreseeable applications aimed at protecting and enhancing the functionalities of biologically active ingredients in food systems.

关键词： Food bioactives Microencapsulation Nanoencapsulation Wall materials Encapsulation techniques Complexation Physicochemical properties Storage stability Biofunctional properties Bioavailability