Tyrosinase Biosensor Used for the Determination of Catechin Derivatives in Tea: Correlation with HPLC/DAD Method
Tyrosinase Biosensor Used for the Determination of Catechin Derivatives in Tea: Correlation with HPLC/DAD Method作者机构：Institut de Modélisation et d’Analyse en Géo-Environnement et Santé Univ. Perpignan Via Domitia Perpignan France Institut de Modélisation et d’Analyse en Géo-Environnement et Santé Univ. Perpignan Via Domitia Perpignan France&Laboratoire d’Analyses Chimiques et Biocapteurs Faculté des Sciences et Techniques Université Hassan II Mohammedia Morocco. Laboratoire d’Analyses Chimiques et Biocapteurs Faculté des Sciences et Techniques Université Hassan II Mohammedia Morocco.
出 版 物：《Food and Nutrition Sciences》 (食品与营养科学（英文）)
年 卷 期：2013年第4卷第1期
页 面：108-118页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Tea Polyphenol Catechin Tyrosinase Amperometric Biosensor HPLC
摘 要：Tea, one of the most popular consumed worldwide beverages, is known to be rich in polyphenols, more particularly in catechins. An amperometric biosensor based on tyrosinase was developed in order to monitor the phenolic content in black and green teas. The enzyme was immobilised by coreticulation with glutaraldehyde on carbon screen-printed electrodes (CSPE). Initially, the performances of the biosensor were evaluated referring to catechol as a model substrate. This analytical tool exhibits a high sensitivity (217 nA/μM), low limit of detection (LOD) = 0.03 μM, good intra-electrode and inter-electrode reproducibilities with RSD lower than 3% (n = 5 injections) and RSD = 8.14% (n = 12 sensors) respectively. The storage stability was also studied;the biosensor retained successively 85% and 70% of its initial response after 34 and 53 days. Subsequently, several catechin derivatives frequently found in teas were tested and classified relatively to their sensitivities. For tea samples, the results obtained with the biosensors were compared to high performance liquid chromatography (HPLC) analysis. A good correlation between the two methods was obtained. The calculated recovery was between 90% and 96%, proving that the proposed tyrosinase biosensor can be an alternative analytical tool for global determination of catechin derivatives in tea.
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