检索结果-南通市图书馆

Phyton-International Journal of Experimental Botany 2023年第9期92卷 2503-2518页

作者： JoséAdrian Perez-Robles carlos Alberto Lecona-guzmán Víctor Manuel Ruíz-Valdiviezo Joaquín Adolfo Montes-Molina Laboratorio de Biotecnología Vegetal Tecnológico Nacional de MexicoCampus Tuxtla GutiérrezTuxtla Gutiérrez29050Mexico

The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum *** with Fusarium oxysporum after the combined application of Beauveria bassiana and plant *** lycopersicum plantlets were transplanted 15 days after the *** days after transplanting,Beauveria bassiana spores were applied at a concentration of 1×10^(7)spores mL^(−1)onto soil(along with ***(n)and ***(H)leaf extracts)where *** plants were *** days after transplanting,spores of *** strain were applied at a concentration of 1×10^(6)spores mL^(−1)to soil where *** plants were *** development of *** plants was monitored for 114 days,whereby a randomized complete block treatment design was used,the roots of the plants were crushed with liquid nitrogen,after which UV/VIS spectrophotometry was used to determine protein concentration,as well as the activity ofβ-1,3-glucanases,peroxidases(POX,EC1.***.1.7),catalase and *** treatments combining *** and *** and *** extracts,had a significant difference in enzymatic activity levels,thus contributing to better defense mechanisms and a greater protection to *** plants in the presence of *** application of *** and plant extracts to the ground mitigated damage caused by *** on *** plants.

关键词： Glucanases chitinases peroxidases catalase defense mechanisms

In vitro Mutagenesis for the Improvement of Agave Genus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2023年第7期92卷 2065-2078页

作者： Sugey Vásquez-Hernández Joaquín Adolfo Montes-Molina Federico Antonio Gutiérrez-Miceli Sheila Jazmín Reyes-Zambrano carlos Alberto Lecona-guzmán Laboratorio de Biotecnología Vegetal TecNM-Campus Tuxtla GutiérrezTuxtla Gutiérrez29050Mexico

Biotechnological techniques provide a viable alternative to help improve and increase the production of plant species of agricultural and economic importance,which have been affected over the years by climate change,increasing their susceptibility to pests and/or diseases,generating losses in production as well as a decrease in their regenerative and genetic *** application of biotechnological techniques such as in vitro mutagenesis offers a viable option for the generation of crops that are resistant to the different factors caused by abiotic and biotic *** vitro mutagenesis has been used in an efficient way to generate genetic changes in different plant ***,these methods have not been studied thoroughly in crops of agro-industrial interest,such as agave,which represents an economic resource of national importance and are considered as endemic species of ***,this literary review aimed to focus on the studies that have been used for the genetic improvement of this species via mutagenesis techniques in plants in the agave ***,the objective was to set a precedent for future genetic studies that aim to obtain more productive regenerants for various industries,such as food and *** is also of great interest to compile information from basic research that helps understand and elucidate a model of possible defense mechanisms that are activated in the Agave genus.

关键词： Biotechnology genetic variations stress mutations resistance

Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2023年第4期92卷 1235-1255页

作者： Henry López-López nancy Ruiz-Lau Rocío Meza-Gordillo Víctor Manuel Ruiz-Valdiviezo Joseph Galthier Robledo-Luchetti carlos Alberto Lecona-guzmán Juan JoséVillalobos-Maldonado Luc Dendooven Joaquín Adolfo Montes-Molina Laboratorio de Biotecnología vegetal Tecnológico Nacional de Mexico/Campus Tuxtla GutiérrezTuxtla Gutiérrez29050Mexico COnACYT-Tecnológico nacional de Mexico/Campus Tuxtla Gutiérrez Tuxtla Gutiérrez29050México ABACUS CinvestavCiudad de México07360México

The objective of this work was to evaluate the effect of Beauveria bassiana(Bb 1205)on controlling Fusarium oxysporum ***(Fol 17108)in tomato plants in greenhouse *** of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymesβ-1,3-glucanases and *** of Bb 1205 occurred at a concentration of 1×108 conidia·mL−1,which was administered onto the leaves,directly into the soil and via *** with Fol 17108 occurred with 1×106 spores·mL−1,which were added directly to the *** was used for measuring agronomic parameters,namely activity of chitinases andβ-1,3-glucanases in foliage and *** Bb 1205 was added to the soil,the chlorophyll index and aerial part length showed significant *** addition,it was determined that root length,fresh weight of foliage,flower,and fruit count increased 82 days after inoculation(dai).Chitinase activity induced by Bb 1205 in leaves and roots of tomato plants infected with Fol 17108 was observed when injected into the stem at 32 dai(41.8 and 11.6-fold,respectively).Inoculation on the foliage showed a 10-fold increase ofβ-1,3-glucanases in the roots after 82 *** for leaves,a 3.8-fold increase was found when the stem was *** the different in vivo applications,Bb 1205 activated its defenses by expressing the chitinase enzymes andβ-1,3-glucanase,thus reducing the damage caused by Fol 17108,demonstrating increase plant growth thereafter.

关键词： Mycoparasitism agronomic parameters chitinases β-1,3-glucanases

The adjustment of γ-aminobutyric acid_A tonic subunits in Huntington＇s disease：from transcription to translation to synaptic levels into the neostriatum

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

neural Regeneration Research 2018年第4期13卷 584-590页

作者： Abraham Rosas-Arellano Argel Estrada-Mondragón Carola A.Mantellero carlos Tejeda-guzmán Maite A.Castro Instituto de Bioquimica y Microbiologia Facultad de Ciencias Universidad Austral de Chile Valdivia Chile Center for Interdisciplinary Studies on the nervous System (CISne) Universidad Austral de Chile Valdivia Chile Departamento de Fisiologla Biofisica y Neurociencias Cinvestav del IPN Ciudad de Mexico Mexico Queensland Brain Institute The University of Queensland Brisbane Australia Laboratorio de neurociencias Departamento de Biologia Facultad de Quimica y Biologla Universidad de Santiago de Chile Santiago de Chile Chile

γ-Aminobutyric acid（GABA）,plays a key role in all stages of life,also is considered the main inhibitory *** activates two kind of membrane receptors known as GABAA and GABAB,the first one is responsible to render tonic inhibition by pentameric receptors containing α4-6,β3,δ,or ρ1-3 subunits,they are located at perisynaptic and/or in extrasynaptic *** biophysical properties of GABAA tonic inhibition have been related with cellular protection against excitotoxic injury and cell death in presence of excessive *** this basis,GABAA tonic inhibition has been proposed as a potential target for therapeutic intervention of Huntington＇s ***＇s disease is a neurodegenerative disorder caused by a genetic mutation of the huntingtin *** experimental studies of Huntington＇s disease mouse models have been developed,such as R6/1,R6/2,Hdh Q92,Hdh Q150,as well as *** all of them,some key experimental reports are focused on *** neostriatum is considered as the most important connection between cerebral cortex and basal ganglia structures,its cytology display two pathways called direct and indirect constituted by medium sized spiny neurons expressing dopamine D1 and D2 receptors respectively,they display strong expression of many types of GABAA receptors,including tonic *** studies about of GABAA tonic subunits and Huntington＇s disease into the neostriatum are rising in recent years,suggesting interesting changes in their expression and localization which can be used as a strategy to delay the cellular damage caused by the imbalance between excitation and inhibition,a hallmark of Huntington＇s disease.

关键词： GABAA extrasynaptic and perisynaptic y-aminobutyric acidA receptors striatum R6/1, R6/2,HdhQ92, HdhQ111, HdhQ150, n171-82Q and YAC128 HD transgenics mice models chorea mutanthuntingtin inhibitory neurotransmission D1 medium sized spiny neurons D2 medium sized spiny neurons

Genetic improvement of wheat grain quality at CIMMYT

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2019年第3期6卷 265-272页

作者： carlos guzmán Karim AMMAR Velu GOVInDAn Ravi SInGH Global Wheat Program The International Maize and Wheat Improvement Center (CIMMYT)

The International Maize and Wheat Improvement Center(CIMMYT) is the global leader in publiclyfunded maize and wheat research and in farming systems based on these *** leads the Global Wheat Program(GWP), which includes some of the largest wheat breeding programs in the *** GWP has been successful in developing wheat germplasm that is used extensively *** quality improvement is a central component of all the breeding efforts at CIMMYT and the Wheat Chemistry and Quality Laboratory represents an integral part of the breeding *** quality is addressed at CIMMYT over the full range of this very wide and variable concept with milling,processing, end-use and nutritional quality *** progenitors and advanced lines developed by the breeders are assessed for diverse quality attributes, with the aim of identifying those that fulfill the requirements in terms of milling, processing, end-use and nutritional quality in different target *** research is conducted to make more efficient the integration of wheat quality traits in the breeding *** main topics being addressed are(1) methodologies to analyze grain quality traits,(2) genetic control and environmental effects on quality traits,(3) characterization of genetic resources for quality improvement, and(4) diversifying grain properties for novel uses.

关键词： biofortification breeding grain quality wheat

Deep optics preconditioner for modulation-free pyramid wavefront sensing

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Photonics Research 2024年第2期12卷 301-312页

作者： FELIPE guzmÁn JORGE TAPIA CAMILO WEInBERGER nICOLÁS HERnÁnDEZ JORGE BACCA BEnOIT nEICHEL ESTEBAn VERA School of Electrical Engineering Pontificia Universidad Católica de Valparaı´soValparaı´soChile Department of Systems Engineering Universidad Industrial de SantanderBucaramangaColombia Laboratoire d’Astrophysique de Marseille MarseilleFrance

The pyramid wavefront sensor(PWFS)can provide the sensitivity needed for demanding adaptive optics applications,such as imaging exoplanets using the future extremely large telescopes of over 30 m of diameter(D).However,its exquisite sensitivity has a limited linear range of operation,or dynamic range,although it can be extended through the use of beam modulation—despite sacrificing sensitivity and requiring additional optical *** by artificial intelligence techniques,this work proposes to train an optical layer—comprising a passive diffractive element placed at a conjugated Fourier plane of the pyramid prism—to boost the linear response of the pyramid sensor without the need for cumbersome *** develop an end-2-end simulation to train the diffractive element,which acts as an optical preconditioner to the traditional least-square modal phase estimation *** results with a large range of turbulence conditions show a noticeable improvement in the aberration estimation performance equivalent to over 3λ∕D of modulation when using the optically preconditioned deep PWFS(DPWFS).Experimental results validate the advantages of using the designed optical layer,where the DPWFS can pair the performance of a traditional PWFS with 2λ∕D of *** and adding an optical preconditioner to the PWFS is just the tip of the iceberg,since the proposed deep optics methodology can be used for the design of a completely new generation of wavefront sensors that can better fit the demands of sophisticated adaptive optics applications such as ground-to-space and underwater optical communications and imaging through scattering media.

关键词： process optics pyramid

Encapsulation of Immature Somatic Embryos of Coffea arabica *** in Vitro Preservation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2021年第6期90卷 1741-1748页

作者： Eliana Arias-Pérez carlos Alberto Lecona-guzmán Federico Antonio Gutiérrez-Miceli Joaquín Adolfo Montes-Molina nancy Ruiz-Lau Laboratorio de Biotecnología Vegetal Instituto Tecnológico de Tuxtla GutiérrezTecnológico Nacional de MéxicoTuxtla GutiérrezChiapas29050México COnACYT-Tecnológico nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez Tuxtla GutiérrezChiapas29050México

The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos(Coffea arabica L.),for the conservation of genotypes with characteristics of commercial *** embryos were induced from leaf explants in Murashige and Skoog medium(MS)supplemented with 1 mg·L^(−1) of 2,4-dichlorophenoxiacetic acid(2,4-D)combined with 2 mg·L^(−1) of benzyladenine(BA).Somatic embryos(SE)at the globular stage were encapsulated in a sodium alginate matrix;two treatments were tested:MS+5 mg·L^(−1) BA+1 mg·L^(−1) nAA+3%(w/v)alginate,and MS+7 mg·L^(−1) BA+5.7 mg·L^(−1) indoleacetic acid(IAA)+3%(w/v)*** was complexed with 100 mM calcium chloride(CaCl_(2)).Viability of the encapsulated SE was determined by staining with 0.01%fluorescein diacetate(FDA)after 0,15,30,and 45 days of storage at 4℃.Embryo viability was 100%in both treatments.

关键词： Coffee explants embryo artificial seeds

Selection and Analysis of Polymorphisms in Somaclonal Variants of Agave americana Resistant to Fusarium oxysporum via an Ethyl Methanesulphonate Treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2021年第6期90卷 1727-1739页

作者： Sheila Jazmín Reyes-Zambrano carlos Alberto Lecona-guzmán Federico Antonio Gutiérrez-Miceli Víctor Manuel Ruiz-Valdiviezo Arely Anayansi Vargas-Díaz Laboratorio de Biotecnología Vegetal Instituto Tecnológico de Tuxtla GutiérrezTecnológico Nacional de MéxicoTuxtla GutiérrezChiapas29050México COnACYT-Colegio de Postgraduados Campus Campeche ChampotónCampeche24450México

Agave americana *** were exposed to different concentrations of ethyl methanesulphonate(EMS)0,15,30,45 and 60 mM and to different times of exposure(2 and 4 h).The viability and capacity of shoot formation were shown to be affected when the callus were exposed to high concentrations(30–60 mM).Only the callus exposed to 15 mM EMS presented shoot formation;the exposure time of two hours produced the largest quantity of shoots regenerated per callus(21 shoots/callus).In order to generate somaclonal variants resistant to Fusarium oxysporum,a selection pressure was applied through of a culture filtrate(CF)of 100 ppm of the *** was made in callus obtained in the treatment with 15 mM EMS during 2 h of *** CF caused oxidation and necrosis in 71.25%of the callus;however,they were capable of generating shoots(3.5 shoots/callus).Molecular markers type RAPD,ISSR and DAMD were used to evaluate the genetic variation arising from the mutations caused by EMS on control plants and 16-month-old somaclonal *** polymorphic information content(PIC)for each one of the initiating groups was:0.28(DAMD),0.09(ISSR)and 0.14(RAPD).DAMD revealed a greater percentage of polymorphism than RAPD and *** bands were detected in the somaclonal *** indicated that the EMS caused genetic variation in the regenerated plants conferring resistance to them against Fusarium oxysporum.

关键词： Mutagenesis polymorphisms genetic variation molecular markers

CO₂ Transformation at Controlled Temperature with Lithium Hydroxide Solution and Metallic Lithium

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Minerals and Materials Characterization and Engineering 2024年第3期12卷 189-203页

作者： Elizabeth Teresita Romero-guzmán José Luis Iturbe-García Departamento de Quí mica Gerencia de Ciencias B&#225sicas Instituto Nacional de Investigaciones Nucleares Carretera M&#233xico-Toluca S/N. La Marquesa Ocoyoac&#225c Estado de M&#233xico Mexico

This paper presents a study on CO2 atmospheric transformation which was reacted directly with lithium hydroxide solution and metallic lithium. This solution was obtained through the reaction between metallic lithium and deionized water where hydrogen is produced and by exposing the metal at ambient conditions. In the transformation process, atmospheric CO2 gas reacts directly with LiOH solution, in both cases, the CO2 transformation kinetics was different. For this purpose, reactions between CO2 and LiOH solution were carried out under controlled temperature and the second process only with metallic lithium, which was exposed at room temperature, however, in these two processes lithium carbonate oxide was formed and identified. According to the results, the efficiency in CO2 transformation is a function of temperature value which was variable until completely obtaining the by-product, its XRD characterization indicated the formation only of Li2CO3 in both procedures. Under laboratory conditions lithium compounds selectively reacted with CO2. In the same way, there is an alternative procedure to obtain LiOH and Li2CO3 for different applications in various areas.

关键词： Metallic Lithium Lithium Hydroxide Solution Hydrogen Atmospheric CO2 Transformation Lithium Carbonate

Gallstone ileus,clinical presentation,diagnostic and treatment approach

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2016年第1期8卷 65-76页

作者： carlos m nuno-guzmán maría eugenia marín-contreras mauricio figueroa-sánchez jorge l corona department of general surgery hospital civil de guadalajara"fray antonio alcalde"Guadalajara CP 44280JaliscoMéxico department of general surgery unidad médica de alta especialidadhospital de especialidades del centro médico nacional de occidenteinstituto mexicano del seguro socialGuadalajara CP 44340JaliscoMéxico department of gastrointestinal endoscopy unidad médica de alta especialidadhospital de especialidades del centro médico nacional de occidenteinstituto mexicano del seguro socialGuadalajara CP 44340JaliscoMéxico department of radiology hospital civil de guadalajara"fray antonio alcalde"Guadalajara CP 44280JaliscoMéxico

Gallstone ileus is a mechanical intestinal obstruction due to gallstone impaction within the gastrointestinal tract. Less than 1% of cases of intestinal obstruction are derived from this etiology. The symptoms and signs of gallstone ileus are mostly nonspecific. This entity has been observed with a higher frequency among the elderly, the majority of which have concomitant medical illness. Cardiovascular, pulmonary, and metabolic diseases should be considered as they may affect the prognosis. Surgical relief of gastrointestinal obstruction remains the mainstay of operative treatment. The current surgical procedures are:(1) simple enterolithotomy;(2) enterolithotomy, cholecystectomy and fistula closure(one-stage procedure); and(3) enterolithotomy with cholecystectomy performed later(two-stage procedure). Bowel resection is necessary in certain cases after enterolithotomy is performed. Large prospective laparoscopic and endoscopic trials are expected.

关键词： Intestinal obstruction Bouveret's syndrome Laparoscopic surgery Endoscopic treatment Gallstoneileus