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Journal of Renewable Materials 2021年第10期9卷 1699-1706页

作者： Judith Constantino-Alcazar Miguel Abud-Archila Benjamín Valdez-Salas Federico Gutierrez-Miceli carlos ceceña-duran Blanca López-Valenzuela Daniel Gonzalez-Mendoza Tecnológico Nacional de México Instituto Tecnológico de Tuxtla GutiérrezTuxtlaGutiérrezC.P.03940México Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC) Ejido Nuevo LeónC.P.21705México Instituto de Ingeniería de la Universidad Autónoma de Baja California Mexicali21100México

In the present study,the green synthesis of potassium nanoparticles(K-NPs)was assessed using aqueous extract of Sideroxylon *** potassium nanoparticles were analyzed by UV-visible spectroscopic techniques,X-ray spectrometers of energy dispersive(SEM-EDS)and dynamic light *** results showed high values at 3.5 keV confirming the formation of potassium nanoparticles and the SEM analysis showed an agglomerated particles size between 360 to 200 nm with a spherical *** K-NPs showed an effective antibacterial activity against the test organisms mainly with Bacillus cereus,Enterobacter aerogenes,Fusarium solani and Botrytis *** further studies about nanotoxicity of K-NPs are needed to confirm their potential in the control of the pathogen microorganisms under field conditions.

关键词： Antimicrobial effect green synthesis Sideroxylon capiri biocontrol

Insecticidal Effect of Green Bimetallic Nanoparticles from Crotalaria longirostrata on Cotton Mealybug,Phenacoccus solenopsis

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Journal of Renewable Materials 2022年第10期10卷 2543-2552页

作者： Vianey Mendez-Trujillo Benjamín Valdez-Salas Mario Curiel-Alvarez Ernesto Beltran-Partida Arnoldo Alfaro-Corres EsaúRuiz-Sanchez Gerardo Bautista-Trujillo Olivia Tzintzun Camacho Onecimo Grimaldo-Juarez carlos ceceña-duran carlos Ail-Catzin Daniel González-Mendoza Facultad de Medicina de la Universidad Autónoma de Baja California Dr.Humberto Torres Sanginés S/NCentro CívicoMexicaliBaja CaliforniaMéxico Instituto de Ingeniería de la Universidad Autónoma de Baja California Calle de la Normal S/N y Boulevard Benito JuárezMexicaliBaja CaliforniaMéxico Instituto Tecnológico de Conkal División de Estudios de Posgrado e InvestigaciónAntigua Carretera Mérida-MotulConkalYucatánMéxico Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de Chiapas ChiapasMéxico Universidad Autónoma de Baja California Instituto de Ciencias AgrícolasBaja CaliforniaMéxico

The present study was conducted to evaluate the impact of bimetallic nanoparticles(NPs)of copper(Cu)and zinc(Zn)from Crotalaria longirostrata on the insect mortality and cell viability of Phenacoccus *** biosynthesis of bimetallic nanoparticles(Cu/Zn-NPs)from *** was confirmed by the presence of a single peak around 378 *** hydrodynamic diameter of the Cu/Zn nanoparticles varied from 59 to 119 nm and their zeta potential was found to be−30.90 *** EDX analysis showed a strong signal for Cu and Zn(8 and 8.6 keV,respectively).The Cu/Zn-NPs showed an effective insecticide activity against *** with 63%of insect mortality after 96 h ***,further studies about nanotoxicity of Cu/Zn-NPs from *** are needed to reveal the exact mechanism of nanoparticles toxicity in insects.

关键词： Phytonanoparticles chipilin biocontrol agronanotechnology

Effects of Crosswalk Location and Pedestrian Volume on Entry Capacity of Roundabouts

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International Journal of Transportation Science and Technology 2013年第1期2卷 31-46页

作者： carlos duran Ruey L Cheu Walter P Moore 221 N Kansas St#601El PasoTX 79901USA The University of Texas at El Paso 500 W University AveEl PasoTX 79968USA

At roundabout approaches,vehicles must yield to pedestrians who are using *** presence of pedestrians using the crosswalk at a roundabout approach thus decreases the entry capacity of the *** research used a calibrated microscopic traffic simulation model to study the effect of crosswalk location and pedestrian volume on the capacity of a two-lane approach entering a two-lane *** simulation results show that(i)at the same pedestrian volume,the crosswalk located further upstream from the yield line causes a smaller magnitude of reduction in the entry capacity,but there is no significant change in the entry capacity when the crosswalk is beyond three car-length upstream from the yield line;(ii)for the same crosswalk location and conflicting volume,the entry capacity reduces with increasing pedestrian volume,but the marginal reduction diminishes with increasing pedestrian *** regression equations for entry capacity adjustment factor for pedestrians as a function of conflicting volume have been *** adjustment factors are found to be lower than the values provided by the Highway Capacity Manual 2010 which are based on research conducted in Germany.

关键词： upstream capacity approach.

Green Synthesis of Bimetallic Nanoparticles From Prosopis juliflora(Sw)DC.,and Its Effect Against Cotton Mealybug,Phenacoccus solenopsis(Hemiptera:Pseudococcidae)

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Phyton-International Journal of Experimental Botany 2019年第3期88卷 269-275页

作者： V.Mendez-Trujillo B.Valdez-Salas M.Carrillo-Beltran M.A.Curiel-Alvarez O.Tzintzun-Camacho C.ceceña-duran D.Gonzalez-Mendoza Facultad de Medicina de la Universidad Autónoma de Baja California Dr.Humberto Torres Sanginés S/NCentro Cívico21000MexicaliBaja CaliforniaMéxico Instituto de Ingeniería de la Universidad Autónoma de Baja California Calle de la Normal S/N y Boulevard Benito Juárez21100MexicaliBaja CaliforniaMéxico Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC).Carretera a Delta S/N C.P.21705 Ejido Nuevo LeónBaja CaliforniaMéxico

Phenacoccus solenopsis has been recognized as an aggressively invasive species on cotton plants in different *** study was conducted to investigate the effect of green synthesized Cu/Zn-nanoparticles using aqueous leaf extract of Prosopis juliflora(mezquite)against *** scanning electron microscope(SEM)showed bimetallic nanoparticles of Cu/Zn-NPs with spherical shape with varying size of 74.33 nm to 59.46 *** than 30%mortality of *** was observed with Cu/Zn-nanoparticles(100 ppm)at 96 hours after *** mortality of *** was recorded with Cu/Zn solution(100 ppm)and aqueous *** *** results of the viability test for Cu/Zn-nanoparticles of *** showed a significant reduction of the cell viability by 50%in insect exposed to Cu/*** studies about nanotoxicity of Cu/Zn-NPs of *** are needed to reveal the mechanism of toxicity this phytonanoparticles in ***.

关键词： Photosynthesis biocontrol cotton mealybug nanoinsecticide

Evaluation of an educational telephone intervention strategy to improve non-screening colonoscopy attendance:A randomized controlled trial

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World Journal of Gastroenterology 2020年第47期26卷 7568-7583页

作者： Agustin Seoane Xenia Font Juan C Perez Rocio Perez carlos F Enriquez Miriam Parrilla Faust Riu Josep M Dedeu Luis E Barranco Xavier duran Ines A Ibanez Marco A Alvarez Digestive Department Endoscopy UnitHospital del MarParc de Salut MarBarcelona 08003Spain Colorectal Cancer Research Group IMIM(Hospital del Mar Medical Research Institute)Barcelona 08003Spain Department of Medicine Autonomous University of BarcelonaBarcelona 08003Spain Consulting Service on Methodology for Biomedical Research IMIM(Hospital del Mar Medical Research Institute)Barcelona 08003Spain

BACKGROUND Colonoscopy attendance is a key quality parameter in colorectal cancer population screening *** these programmes,educative interventions with bidirectional contact carried out by trained personnel have been proved to be an important tool for colonoscopy attendance improvement,and because of its huge clinical and economic impact,they have been widely ***,outside of this population programmes,educative measures to improve colonoscopy attendance have been poorly studied and no navigation interventions are usually *** To investigate the clinical and economic impacts of an educational telephone intervention on colonoscopy attendance outside colorectal cancer screening *** This randomized controlled trial included consecutive patients referred to colonoscopy from primary care centres from November 2017 to May *** intervention group(IG)received a telephone intervention,while the control group(CG)did *** assigned to the IG received an educational telephone call 7 d before the colonoscopy *** intervention was carried out by two nurses with deep endoscopic knowledge who were previously trained for a telephone educational intervention for *** impact on patient compliance with preparedness protocols related to bowel cleansing,antithrombotic management,and sedation scheduling was also evaluated.A second call was conducted to assess patient ***-to-treat(ITT)and perprotocol(PP)analyses were *** A total of 738 and 746 patients were finally included in the IG and CG *** hundred thirteen(83%)patients were contacted in the *** non-attendance rate was lower in the IG,both in the ITT analysis(IG 8.4%vs CG 14.3%,P<0.001)and in the PP analysis(4.4%vs 14.3%,P<0.001).In a multivariable analysis,belonging to the control group increased the risk of nonattendance in both,the ITT analysis(OR 1.81,95%CI:1.27 to 2.58,P=0.001)and the PP analysis(O

关键词： Colonoscopy Quality improvement No-show patients Nursing education Patient compliance Telephone intervention

Dietary xylanase and live yeast supplementation influence intestinal bacterial populations and growth performance of piglets fed a sorghum-based diet

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Animal Nutrition 2020年第4期6卷 457-466页

作者： Gemma González-Ortiz Marco A.Callegari Pete Wilcock Diego Melo-duran Michael R.Bedford Hilário R.V.Oliveira Marcos A.A.da Silva carlos R.Pierozan Caio A.da Silva AB Vista MarlboroughWiltshireSN84ANUnited Kingdom AKEI Animal Research Fartura18870000Brazil Servei de Nutricio i Benestar Animal(SNiBA) Department de Ciencia Animal i dels AlimentsUniversitat Autonoma de Barcelona(UAB)08193Spain Universidade Estadual Norte do Parana Bandeirantes86360000Brazil Universidade Estadual de Londrina Londrina86051990Brazil

This study was to evaluate the effect of xylanase supplementation and the addition of live yeast,Saccharomyces cerevisiae,on growth performance and intestinal microbiota in *** hundred and eighty commercial crossbred 23-d-old piglets(PIC 417)were sorted by initial BW and allocated to 3 treatments:control(CTR)diet,CTR diet supplemented with xylanase at 16,000 birch xylan units/kg(XYL)and XYL diet supplemented with live yeast(2 x 1010 CFU/g)at 1 kg/t(XYL+LY).Each treatment had 10 replicates,with 6 animals each.A sorghum-based diet and water were available ad libitum for 42 d of the *** daily gain(ADG)and average daily feed intake(ADFI)were measured from 0 to 42 d(23-to 65-d-old)and feed conversion ratio(FCR)*** the end of the study,bacterial identification through 16 S rRNA(V3 to V4)sequencing of the ileal and caecal digesta from one piglet per replicate was *** treatment effects were observed on *** offered the live yeast in addition to the xylanase had increased ADG compared with those supplemented with xylanase alone(XYL+LY ***;P=0.655).FCR was improved with XYL and XYL+LY compared with CTR(P=0.018).Clostridiaceae counts in the ileum tended to reduce by 10%with XYL and 14%with XYL+LY compared to CTR(P=0.07).XYL and XYL+LY increased the counts of Lactobacillaceae in the caecum compared with CTR(P<0.0001).Dietary supplementation of live yeast combined with xylanase improved growth performance and microbial balance of piglets during the nursery phase.

关键词： Sorghum Nursery piglet Xylanase Live yeast Growth performance Microbiota

Combination of squamous cell carcinoma antigen immunocomplex and alpha-fetoprotein in mid-and long-term prediction of hepatocellular carcinoma among cirrhotic patients

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World Journal of Gastroenterology 2021年第48期27卷 8343-8356页

作者： Antonio Gil-Gómez Ángela Rojas Chang-Hai Liu Rocio Gallego-duran Rocio Muñoz-Hernandez Giorgio Fassina Patrizia Pontisso Javier Ampuero Manuel Romero-Gómez SeLiver Group Institute of Biomedicine of SevilleSeville 41013Spain CIBERehd Instituto de Salud Carlos IIIMadrid 28029Spain Mucosal Immunity Lab IRCCS Humanitas Research HospitalMilan 20089Italy Center of Infectious Diseases West China Hospital of Sichuan UniversityChengdu 610017Sichuan ProvinceChina State Key Laboratory of Biotherapy and Center of Infectious Diseases West China HospitalChengdu 610017Sichuan ProvinceChina Life Biotechnology Padua UniversityVenice 30175Italy Department of Clinical and Experimental Medicine University of PadovaPadova 35123Italy UCM Digestive Diseases Virgen del Rocío University HospitalSeville 41014Spain

BACKGROUND The combination of alpha-fetoprotein(AFP)and squamous cell carcinoma antigen immunocomplex(SCCA-IgM)have been proposed for its use in the screening of hepatocellular carcinoma(HCC).Current screening programs for all cirrhotic patients are controversial and a personalized screening is an unmet need in the precision medicine *** To determine the role of the combination of SCCA-IgM and AFP in predicting mid-and long-term appearance of *** Two-hundred and three cirrhotic patients(Child A 74.9%,B 21.2%,C 3.9%)were followed-up prospectively every six months to screen HCC by ultrasound and AFP according to European Association for the Study of the Liver *** estimation cohort was recruited in Italy(30.5%;62/203)and validation cohort from Spain(69.5%;141/203).Patients underwent to evaluate SCCA-IgM by enzyme-linked immunosorbent assay(Hepa-IC,Xeptagen,Italy)and AFP levels at *** were followed-up for 60 mo,being censored at the time of the appearance of *** There were 10.8%and 23.1%of HCC development at two-and five-years *** with HCC showed higher levels of SCCA-IgM than those without it(425.72±568.33 AU/mL vs 195.93±188.40 AU/mL,P=0.009)during the fiveyear *** multivariate analysis,after adjusting by age,sex,aspartate transaminase and Child-Pugh,the following factors were independently associated with HCC:SCCA-IgM[Hazard ratio(HR)=1.001,95%CI:1.000-1.002;P=0.003],AFP(HR=1.028,95%CI:1.009-1.046;P=0.003)and creatinine(HR=1.56495%CI:1.151-2.124;P=0.004).The log-rank test of the combination resulted in 7.488(P=0.024)in estimation cohort and 11.061(P=0.004)in the validation cohort,and a 100%of correctly classified rate identifying a low-risk group in both cohorts in the two-year *** We have constructed a predictive model based on the combination of SCCA-IgM and AFP that provides a new HCC screening method,which could be followed by tailored HCC surveillance for individual patients,esp

关键词： Squamous cell carcinoma antigen Hepatocellular carcinoma prediction Precision medicine Stratification of cirrhotic patient

Avocado Nutritional Diagnosis （Persea americana Mill.）＂Hass＂, Soil Fertility and Water Quality in Ocuituco, Morelos

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Journal of Chemistry and Chemical Engineering 2013年第11期7卷 1068-1073页

作者： Hector Sotelo-Nava Oscar Gabriel Villegas-Torres Martha Lilia Dominguez-Patint Elias Hernfindez Castro Agustin Damian Nava Antonio Rodrlguez-Martmez carlos Manuel Acosta-duran Maria Andrade-Rodriguez Iran Alia-Tej acal Faculty of Agricultural Sciences Universidad Aut6noma del Estado de Morelos Cuernavaca 62209 Mexico Faculty of Chemical Sciences and Engineering Universidad Aut6noma del Estado de Morelos Cuernavaca 62209 Mexico Unit of Agricultural and Environmental Sciences Universidad Autonoma de Guerrero Guerrero 39196 Mexico Engineering and Applied Science Research Center Universidad Autonoma del Estado de Morelos Cuernavaca 62209 Mexico

Morelos is the fourth in area cultivated and production volume of avocado. Of the 13 municipalities where it is farmed, Ocuituco represents 43.51% and Tetela del Volain 27.53%. Despite being one of the main fruit species in Morelos, no information related to the fertility of the soil where it is grown, the quality of water available for irrigation and nutritional status of this important fruit for the state. The study was conducted in the municipality of Ocuituco which identified three agro-habitats taking into account climate, landform, soil type and altitude. In each agro-habitat were carried out soil sampling to determine the physical and chemical characteristics based on the Mexican Official Standard NOM-021-SEMARNAT-2000, the chemical quality of water for irrigation as described by Richards （1954） and crop nutrient status of avocado ＂Hass＂ according to the methodology described by Maldonado （2002）. The soils had different physical and chemical characteristics, as well as the nutritional status of avocado trees in the three agro-habitats. The trees had excessive concentration of CI, Cu and Zn. Indices of optimal percentage deviation expressed different nutritional requirements. The water used for irrigation was of low salinity and sodium content, so it can be used without restrictions.

关键词： Soil fertility water quality nutrients Hass.

Controlling self-assembling and tumor cell-targeting of protein-only nanoparticles through modular protein engineering

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Science China Materials 2020年第1期63卷 147-156页

作者： Eric Volta-duran Olivia Cano-Garrido Naroa Serna Hector Lopez-Laguna Laura Sanchez-Garcia Mireia Pesarrodona Alejandro Sanchez-Chardi Ramon Mangues Antonio Villaverde Esther Vazquez Ugutz Unzueta Institut de Biotecnologia i de Biomedicina Universitat Autònoma de BarcelonaBellaterra08193 BarcelonaSpain Departament de Genetica i de Microbiologia Universitat Autònoma de BarcelonaBellaterra08193 BarcelonaSpain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)C/Monforte de Lemos 3-528029 MadridSpain Nanoligent SL Edifici EUREKAUniversitat Autonoma de BarcelonaBellaterra08193 BarcelonaSpain Institut dInvestigacions Biomèdiques Sant Pau and Josep Carreras Research Institute Hospital de la Santa Creu i Sant Pau08041 BarcelonaSpain Departament de Biologia Evolutiva Ecologia i Ciencies AmbientalsFacultat de BiologiaUniversitat de BarcelonaAv.Diagonal 64308028 BarcelonaSpain Institute for Research in Biomedicine(IRB Barcelona) The Barcelona Institute of Science and Technology08028 BarcelonaSpain

Modular protein engineering is suited to recruit complex and multiple functionalities in single-chain polypeptides. Although still unexplored in a systematic way, it is anticipated that the positioning of functional domains would impact and refine these activities, including the ability to organize as supramolecular entities and to generate multifunctional protein materials. To explore this concept, we have repositioned functional segments in the modular protein T22-GFP-H6 and characterized the resulting alternative fusions. In T22-GFP-H6, the combination of T22 and H6 promotes selfassembling as regular nanoparticles and selective binding and internalization of this material in CXCR4-overexpressing tumor cells, making them appealing as vehicles for selective drug delivery. The results show that the pleiotropic activities are dramatically affected in module-swapped constructs, proving the need of a carboxy terminal positioning of H6 for protein self-assembling, and the accommodation of T22 at the amino terminus as a requisite for CXCR4^+ cell binding and internalization. Furthermore, the failure of self-assembling as regular oligomers reduces cellular penetrability of the fusions while keeping the specificity of the T22-CXCR4 *** these data instruct how multifunctional nanoscale protein carriers can be designed for smart, protein-driven drug delivery, not only for the treatment of CXCR4^+ human neoplasias, but also for the development of anti-HIV drugs and other pathologies in which CXCR4 is a relevant homing marker.

关键词： nanoparticles protein materials recombinant proteins drug delivery self-assembling cancer cell targeting

Beneficial effects of saffron(Crocus sativus L.) in ocular pathologies, particularly neurodegenerative retinal diseases

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Neural Regeneration Research 2020年第8期15卷 1408-1416页

作者： Jose A.Fernandez-Albarral Rosa de Hoz Ana I.Ramirez Ines Lopez-Cuenca Elena Salobrar-Garcia Maria D.Pinazo-duran Jose M.Ramirez Juan J.Salazar Instituto de Investigaciones Oftalmologicas Ramon Castroviejo MadridSpain Departamento de Inmunologia Oftalmologia y ORLFacultad de Optica y OptometriaMadridSpain Departamento de Inmunologia Oftalmologia y ORLFacultad de MedicinaMadridSpain Unidad de Investigacion Oftalmologica Santiago Grisolia Universidad de ValenciaValenciaSpain

Saffron(Crocus sativus L.)has been traditionally used in food preparation and as a medicinal *** currently has numerous therapeutic properties attributed to it,such as protection against ischemia,as well as anticonvulsant,antidepressant,anxiolytic,hypolipidemic,anti-atherogenic,anti-hypertensive,antidiabetic,and anti-cancer *** addition,saffron has remarkable beneficial properties,such as anti-apoptotic,anti-inflammatory and antioxidant activities,due to its main metabolites,among which crocin and crocetin stand ***,increasing evidence underwrites the possible neuroprotective role of the main bioactive saffron constituents in neurodegenerative diseases,such as Parkinson’s and Alzheimer’s diseases,both in experimental models and in clinical studies in ***,saffron supplementation is being tested for ocular neurodegenerative pathologies,such as diabetic retinopathy,retinitis pigmentosa,age-related macular degeneration and glaucoma,among others,and shows beneficial *** present article provides a comprehensive and up to date report of the investigations on the beneficial effects of saffron extracts on the main neurodegenerative ocular pathologies and other ocular *** review showed that saffron extracts could be considered promising therapeutic agents to help in the treatment of ocular neurodegenerative diseases.

关键词： AMD crocetin crocin Crocus sativus L. diabetic retinopathy glaucoma neuroprotection ocular diseases retinitis pigmentosa saffron safranal