检索结果-南通市图书馆

Screening of the ubiquitin-proteasome system activators for anti-Alzheimer’s disease by the high-content fluorescence imaging system

引用

Chinese Journal of Natural Medicines 2022年第1期20卷 33-42页

作者： WANG Yi-Ling YOU jing cao jing-jie LI Wei jing Liu-Yang MEI Qi-Bing WU An-Guo Sichuan Key Medical Laboratory of New Drug Discovery and Drugability Evaluation Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia MedicaSchool of PharmacyEducation Ministry Key Laboratory of Medical ElectrophysiologySouthwest Medical UniversityLuzhou 646000China Luzhou New Drug Evaluation and Research Center Luzhou 646000China

Ubiquitin-proteasome system(UPS)plays an important role in neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),and Huntington’s disease(HD).The discovery of UPS activators for anti-neurodegenerative diseases is becoming increasingly *** this study,we aimed to identify potential UPS activators using the high-throughput screening method with the high-content fluorescence imaging system and validate the neuroprotective effect in the cell models of *** first,stable YFP-CL1 HT22 cells were successfully constructed by transfecting the YFP-CL1 plasmid into HT22 cells,together with G418 *** degradation activity of the test compounds via UPS was monitored by detecting the YFP fluorescence intensity reflected by the ubiquitin-proteasome degradation signal *** employing the high-content fluorescence imaging system,together with stable YFP-CL1 HT22 cells,the UPS activators were successfully screened from our established TCM *** representative images were captured and analyzed,and quantification of the YFP fluorescence intensity was performed by flow ***,the neuroprotective effect of the UPS activators was investigated in pEGFP-N1-APP(APP),pRK5-EGFP-Tau P301L(Tau P301L),or pRK5-EGFP-Tau(Tau)transiently transfected HT22 cells using fluorescence imaging,flow cytometry,and Western *** conclusion,our study established a high-content fluorescence imaging system coupled with stable YFP-CL1 HT22 cells for the highthroughput screening of the UPS *** compounds,namely salvianolic acid A(SAA),salvianolic acid B(SAB),and ellagic acid(EA),were identified to significantly decrease YFP fluorescence intensity,which suggested that these three compounds are UPS *** identified UPS activators were demonstrated to clear AD-related proteins,including APP,Tau,and Tau ***,these findings provide a novel insight into the discovery and development of anti-AD drugs.

关键词： Ubiquitin-proteasome system High-content fluorescence imaging system Traditional Chinese medicine Alzheimer’s disease YFP-CL1 HT22 cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于Curvelet变换与POCS方法的三维数字岩心重建

引用

地球物理学报 2015年第6期58卷 2069-2078页

作者：王本锋李景叶陈小宏曹静杰中国石油大学(北京)油气资源与探测国家重点实验室北京102249 中国石油大学(北京)CNPC物探重点实验室北京102249 石家庄经济学院石家庄050031

随着页岩气勘探与开发的深入,研究页岩裂隙的三维空间展布成为页岩岩石物理研究的必要步骤之一.但由于仪器的限制,页岩切片在深度上具有不连续性,以及数字岩心纵向上成像最小间隔与横向分辨率的不一致成为影响裂隙表征和数字岩石物理模... 详细信息

随着页岩气勘探与开发的深入,研究页岩裂隙的三维空间展布成为页岩岩石物理研究的必要步骤之一.但由于仪器的限制,页岩切片在深度上具有不连续性,以及数字岩心纵向上成像最小间隔与横向分辨率的不一致成为影响裂隙表征和数字岩石物理模拟精度提高的重要因素.为了更好的研究裂隙在三维的空间展布,本文将curvelet稀疏变换与凸集投影(POCS)迭代算法有效结合,实现三维数字岩心重建.首先对X射线扫描砂岩得到的三维数据体进行隔片抽稀,利用本文方法实现三维数据体重建,重建结果与完整数据体具有很好的一致性,且优于现有方法(spgl1),验证了新方法的有效性与先进性.其次对聚焦离子束扫描电镜(FIB-SEM)得到的纳米级页岩二维切片在深度上进行了加密重建,获得纵向上成像最小间隔与横向分辨率基本一致的三维数字岩心,由于仪器限制引起的页岩切片深度上的不连续性得到减弱,裂隙展布更加清晰.砂岩CT图像以及页岩FIB-SEM成像数据的重建结果验证了本文方法的有效性与先进性.

关键词： Curvelet变换凸集投影(POCS) 三维数字岩心页岩重建

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of the extrusion parameters on microstructure,texture and room temperature mechanical properties of extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy

引用

International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 136-146页

作者： Chenjin Zhang Guangyu Yang Lei Xiao Zhiyong Kan jing Guo Qiang Li Wanqi jie State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China

Microstructure,texture,and mechanical properties of the extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy were investigated at different extrusion temperatures(260 and 320℃),extrusion ratios(10:1,15:1,and 30:1),and extrusion speeds(3 and 6 mm/s).The experimental results exhibited that the grain sizes after extrusion were much finer than that of the homogenized alloy,and the second phase showed streamline distribution along the extrusion direction(ED).With extrusion temperature increased from 260 to 320℃,the microstructure,texture,and mechanical properties of alloys changed *** dynamic recrystallization(DRX)degree and grain sizes enhanced as the extrusion ratio increased from 10:1 to 30:1,and the strength gradually decreased but elongation(EL)*** the extrusion speed increased from 3 to 6 mm/s,the grain sizes and DRX degree increased significantly,and the samples presented the typical-rare-earth(RE)*** alloy extruded at 260℃ with extrusion ratio of 10:1 and extrusion speed of 3 mm/s showed the tensile yield strength(TYS)of 213 MPa and EL of 30.6%.After quantitatively analyzing the contribution of strengthening mechanisms,it was found that the grain boundary strengthening and dislocation strengthening played major roles among strengthening *** results provide some guidelines for enlarging the industrial application of extruded Mg-RE alloy.

关键词： Mg-rare earth alloys extrusion temperature extrusion ratio extrusion speed strengthening mechanisms

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

地震数据压缩重构的正则化与零范数稀疏最优化方法

引用

地球物理学报 2012年第2期55卷 596-607页

作者：曹静杰王彦飞杨长春石家庄经济学院石家庄050031 油气资源研究重点实验室中国科学院地质与地球物理研究所北京100029 中国科学院研究生院北京100049

地震数据重构问题是一个病态的反演问题.本文基于地震数据在curvelet域的稀疏性,将地震数据重构变为一个稀疏优化问题,构造0范数的逼近函数作为目标函数,提出了一种投影梯度求解算法.本文还运用最近提出的分段随机采样方式进行采样,该... 详细信息

地震数据重构问题是一个病态的反演问题.本文基于地震数据在curvelet域的稀疏性,将地震数据重构变为一个稀疏优化问题,构造0范数的逼近函数作为目标函数,提出了一种投影梯度求解算法.本文还运用最近提出的分段随机采样方式进行采样,该采样方式能够有效地控制采样间隔并且保持采样的随机性.地震数值模拟表明,基于0范数逼近的投影梯度法计算效率有明显的提高;分段随机采样方式比随机欠采样有更加稳定的重构结果.

关键词：波场重构 curvelet变换压缩传感 0范数逼近反问题不适定性稀疏优化

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

梯级水电站实时补偿增益多因素综合分配方法探讨

引用

水力发电学报 2008年第6期27卷 22-26页

作者：钟平安曹静蔡杰河海大学水文水资源学院南京210098

龙头水库带一串日调节或无调节电站组成的水电梯级,在汛初和汛期两场洪水之间的特定时段开展小流量泄流补偿调度,既可以减少龙头水库无益弃水,又能增加下游电站发电量;分析了影响实时补偿调度增益分配的主要因素;提出了补偿调度增益的... 详细信息

龙头水库带一串日调节或无调节电站组成的水电梯级,在汛初和汛期两场洪水之间的特定时段开展小流量泄流补偿调度,既可以减少龙头水库无益弃水,又能增加下游电站发电量;分析了影响实时补偿调度增益分配的主要因素;提出了补偿调度增益的计算原理和龙头水库泄流补偿调度风险率与期望损失计算方法;提出了具有明确物理意义的补偿调度增益多因素综合分配模型。通过沙溪流域的实例分析,表明提出的方法能够取得合理的结果。

关键词：水利经济增益分配梯级水电站实时补偿调度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Unraveling post-growth mechanisms of monolayer CsPbBr3 nanocubes:Laser-enhanced transformations and cathodoluminescence-electron microscopy correlations

引用

Journal of Energy Chemistry 2025年第1期100卷 146-156页

作者： Mingxing Li Xiaoge Wang Xiaofan cao Zhiqun He Chunjun Liang Mingxing Chen jing Ju Fangtian You Key Laboratory of Luminescence and Optical Information Ministry of Education Institute of Optoelectronic Technology Beijing Jiaotong University Beijing National Laboratory for Molecular Sciences (BNLMS) College of Chemistry and Molecular Engineering Peking University

Lead-halide perovskite nanoparticles（LHP NPs） are highly promising materials for next-generation displays and solid-state lighting due to their exceptional optical properties. However, their inherent instability presents a significant challenge. Recent advances have demonstrated that optoelectronic devices based on monolayer nanoparticle films exhibit both high luminescence efficiency and long-term *** research demonstrates that mobility limitations and anisotropic alignments in CsPbBr3nanocube monolayer films are key to their stabilization, hindering spontaneous growth through face-to-face fusion and resulting in the formation of connecting necks in a diagonal direction. Introducing laser irradiation confirmed this by significantly accelerating nanocubes growth, increasing mobility, and enhancing local structural ordering, leading to larger and more regularly shaped nanosheets. Fourier transform infrared spectroscopy and energy dispersive spectroscopy line-scan analyses indicated that laser irradiation did not disrupt the ligand structure. Transmission electron microscopy and correlative cathodoluminescence electron microscopy revealed the effects of post-growth and heterogeneous structures, including enhanced luminescence and inhomogeneous intensity in the nanosheets. These findings deepen the understanding of the post-growth mechanism of monolayer nanoparticles and the structure-emission correlation and highlight the unique role of laser irradiation in directing the formation of well-defined and regular nanostructures.

关键词： Energy dispersive spectroscopy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Correlation between Combined Urinary Metal Exposure and Grip Strength under Three Statistical Models:A Cross-sectional Study in Rural Guangxi

引用

Biomedical and Environmental Sciences 2024年第1期37卷 3-18页

作者： LIANG Yu Jian RONG Jia Hui WANG Xue Xiu CAI Jian Sheng QIN Li Dong LIU Qiu Mei TANG Xu MO Xiao Ting WEI Yan Fei LIN Yin Xia HUANG Shen Xiang LUO Ting Yu GOU Ruo Yu cao jie jing HUANG Chu Wu LU Yu Fu QIN Jian ZHANG Zhi Yong Department of Environmental and Occupational Health School of Public HealthGuangxi Medical UniversityNanning 530021GuangxiChina Key Laboratory of Tumor Immunology and Microenvironmental Regulation Guilin Medical UniversityGuilin 541199GuangxiChina Department of Environmental Health and Occupational Medicine School of Public HealthGuilin Medical UniversityGuilin 541199GuangxiChina Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases Guangxi Medical UniversityNanning 530021GuangxiChina Guangxi Key Laboratory of Environment and Health Research Guangxi Medical UniversityNanning 530021GuangxiChina Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education Guangxi Medical UniversityNanning 530021GuangxiChina Guangxi Health Commission Key Laboratory of Entire Lifecycle Health and Care Guilin Medical UniversityGuilin 541199GuangxiChina

Objective This study aimed to investigate the potential relationship between urinary metals copper(Cu),arsenic(As),strontium(Sr),barium(Ba),iron(Fe),lead(Pb)and manganese(Mn)and grip *** We used linear regression models,quantile g-computation and Bayesian kernel machine regression(BKMR)to assess the relationship between metals and grip *** In the multimetal linear regression,Cu(β=−2.119),As(β=−1.318),Sr(β=−2.480),Ba(β=0.781),Fe(β=1.130)and Mn(β=−0.404)were significantly correlated with grip strength(P<0.05).The results of the quantile g-computation showed that the risk of occurrence of grip strength reduction was−1.007(95%confidence interval:−1.362,−0.652;P<0.001)when each quartile of the mixture of the seven metals was *** kernel function regression model analysis showed that mixtures of the seven metals had a negative overall effect on grip strength,with Cu,As and Sr being negatively associated with grip strength *** the total population,potential interactions were observed between As and Mn and between Cu and Mn(P_(interactions) of 0.003 and 0.018,respectively).Conclusion In summary,this study suggests that combined exposure to metal mixtures is negatively associated with grip ***,Sr and As were negatively correlated with grip strength levels,and there were potential interactions between As and Mn and between Cu and Mn.

关键词： Urinary metals Handgrip strength Quantile g-computation Bayesian kernel machine regression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

一种基于压缩感知的地震数据重建方法及其在城市活断层地震勘探中的应用

引用

地球物理学进展 2020年第4期35卷 1545-1551页

作者：曹静杰杨志权杨歧焱河北地质大学河北省战略性关键矿产资源重点实验室石家庄050031

在城市地区采用地震勘探方法时,由于地表障碍物、地形等因素的影响,地震检波器不能按照观测系统设计规则布设,采集的地震数据存在道缺失,常规的浅层地震数据处理方法由于没有采用数据重建方法,因此会影响地震数据处理和解释的效果.压缩... 详细信息

在城市地区采用地震勘探方法时,由于地表障碍物、地形等因素的影响,地震检波器不能按照观测系统设计规则布设,采集的地震数据存在道缺失,常规的浅层地震数据处理方法由于没有采用数据重建方法,因此会影响地震数据处理和解释的效果.压缩感知技术被引入到地震勘探中已经有十余年,但都是在石油地震勘探中应用,其原因是石油地震勘探数据量大,信噪比高.由于浅层地震勘探的覆盖次数小,信噪比低,在浅层地震勘探中应用压缩感知技术存在一定的挑战,能否应用压缩感知技术于城市浅层地震勘探尚未可知.本文对压缩感知理论在浅层地震勘探中的应用进行探索,将模拟的压缩感知采集的数据进行重建;以曲波变换为稀疏变换,通过构造0-范数的一种逼近函数建立稀疏反演模型,并提出了一种快速的求解方法.模拟数据实验表明,本文方法能够很好的对缺失地震数据进行重建.对某地区地震活断层勘探的实际地震数据进行了模拟的压缩感知采样和数据重建,并对原始数据、压缩感知采集数据和重建数据分别进行了相同的地震数据处理,偏移成像结果验证了基于压缩感知的重建方法能够获得和常规采集数据相当的处理结果.本文验证了基于压缩感知的地震数据重建技术在浅层地震勘探中的可行性,为后续的研究打下了良好的基础.

关键词：城市活断层压缩感知曲波变换地震数据重建零范数逼近

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DVB-MMA共聚物树脂吸附水相苯酚的研究

引用

中国环境科学 2013年第8期33卷 1386-1391页

作者：詹德利卢晗锋周瑛曹洁晶陈银飞浙江工业大学化学工程与材料科学学院催化反应工程研究所浙江杭州310014

以二乙烯基苯(DVB)和甲基丙烯酸甲酯(MMA)的混合液为单体,分别采用悬浮聚合法和溶剂热法合成高分子树脂PDMA和PDVB.通过BET、IR、SEM对样品的结构和形貌进行表征,对比2种方法合成吸附树脂的性能差异,并考察各高分子树脂对水中苯酚的吸... 详细信息

以二乙烯基苯(DVB)和甲基丙烯酸甲酯(MMA)的混合液为单体,分别采用悬浮聚合法和溶剂热法合成高分子树脂PDMA和PDVB.通过BET、IR、SEM对样品的结构和形貌进行表征,对比2种方法合成吸附树脂的性能差异,并考察各高分子树脂对水中苯酚的吸附效果.结果表明,采用悬浮聚合法制备的DVB-MMA共聚物树脂在2-4nm和5-30nm范围内均有明显孔径分布,具有较大的比表面积566m2/***-MMA共聚物树脂(PDMA-XF)对4500mg/L苯酚溶液的饱和吸附量为170.4mg/g,其酯羰基中的氧原子与酚羟基当中的氢原子产生氢键作用,这些分子间的氢键作用有助于吸附水中苯酚.

关键词：树脂吸附苯酚二乙烯基苯甲基丙烯酸甲酯

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

引用

Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong jing-jing Hu Yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-jie Zhu Xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：