检索结果-南通市图书馆

Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

引用

Journal of Integrative Agriculture 2018年第10期17卷 2281-2291页

作者： LIU Wen-wen XIN Min cao meng-ji QIN meng LIU Hui ZHAO Shou-qi WANG Xi-feng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China National Citrus Engineering Research Center Citrus Research Institute Southwest University Chongqing 400712 P.R. China National Agricultural Technology Extension and Service Center Beijing 100125 P.R. China

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA（dsRNA） molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus（HaEV）. Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region（UTR）, 3＇ UTR ending with the unique sequence CCCCCCCC and lacking a poly（A） tail. An open reading frame（ORF） that encodes a deduced 4 867 amino acids（aa） polyprotein with three domains： RdRP, Hel and UGT（UDP-glycosyltransferase）. HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization（FISH） experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.

关键词： common sunflower (Helianthus annuus) next-generation sequencing (NGS) double-stranded RNA Endornaviridae

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of a novel emaravirus infecting lilac through next-generation sequencing

引用

Journal of Integrative Agriculture 2020年第8期19卷 2064-2071页

作者： WANG Ya-qin SONG Yu cao meng-ji CHENG Qi WU jian-xiang HU Tao State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058P.R.China National Citrus Engineering and Technology Research Center Citrus Research InstituteSouthwest UniversityChongqing 400712P.R.China

Emaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering *** unbiased next-generation sequencing(NGS)approach,a novel virus from lilac leaves with yellow mottle symptom was *** viral genomic RNA segments were detected and completely *** molecular characteristics of the viral RNAs and proteins resemble those of members of the genus *** virus was found to be mechanically transmissible to Nicotiana *** alignment and phylogenetic analyses of the virus support the proposal of creating a new species in the genus Emaravirus for which the name lilac chlorotic ringspot-associated virus(LiCRaV)is proposed.

关键词： LiCRaV lilac Fimoviridae Emaravirus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Small RNA deep sequencing reveals full-length genome of Citrus yellow vein clearing virus in Chongqing,China

引用

Journal of Integrative Agriculture 2017年第2期16卷 503-508页

作者： YU Yun-qi WU Qiong SU Hua-nan WANG Xue-feng cao meng-ji ZHOU Chang-yong College of Plant Protection Southwest University National Citrus Engineering and Technology Research Center Citrus Research InstituteSouthwest University/Chinese Academy of Agricultural Sciences National Navel Orange Engineering and Technology Research Center Gannan Normal University

To identity the potential pathogen associated with the yellow vein clearing symptom on lemon trees, the profiles of virus-de- rived small interfering RNAs from citrus samples were obtained and analyzed by deep sequencing method in this study. Twenty-seven contigs almost cover the full length genome of Citrus yellow vein clearing virus （CYVCV） isolate YN were obtained using the small RNA deep sequencing technology. Analysis showed that this isolate CQ shared the highest nucleotide identity with isolate Y1 （JX040635） and YN （KP313242）, both of which belong to the genus Mandarivirus in the family Alphaflexiviridae. Mapping analysis of viral-derived siRNA （vsiRNA） profiles revealed an uneven distribution pattern of their generations along both positive and negative genome strands, and 22- and 21-nt vsiRNAs ranked the majority. BLAST against viroids and other viral databases confirmed that this sample was single-infected only by CYVCV, which indicated that CYVCV was the exact causal agent for the yellow clearing symptom occurring on lemon. This is the first CYVCV isolate detected in Chongqing and the second in China. This result could provide a molecular basis for the investigation of citrus viral diseases to protect citrus health in this region.

关键词： citrus Citrus yellow vein clearing virus (CYVCV) small RNA deep sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China

引用

Journal of Integrative Agriculture 2018年第3期17卷 712-715页

作者： LI Ping LI Min ZHANG Song WANG Jun YANG Fang-yun cao meng-ji LI Zhong-an National Citrus Engineering Research Center Citrus Research Institute Southwest University College of Agronomy and Biotechnology Southwest University

Citrus leaf blotch virus （CLBV） is a member of the genus Citrivirus, in the family Betaflexiviridae. It has been reported CLBV could infect kiwi, citrus and sweet cherry in China. Of 289 citrus samples from six regions of China, 15 were detected to be infected with CLBV in this study. The complete genome of four isolates of CLBV was obtained from Reikou in Sichuan （CLBV-LH）, Yura Wase in Zhejiang （CLBV-YL）, Bingtangcheng in Hunan （CLBV-BT）, Fengjie 72-1 in Chongqing （CLBV- F J）, respectively. While they all represented 8 747 nucleotides in monopartite size, excluding the poly（A） tail, each of the isolates coded three open reading frames （ORFs）. Identity of the four isolates ranged from 98.9 to 99.8% to each other and from 96.8 to 98.1% to the citrus references in GenBank by multiple alignment of genomes. A phylogenetic tree based on the genome sequences of available CLBV isolates indicated that the four isolates were clustered together, suggesting that CLBV isolates from citrus in China did not have obvious variation. This is the first report of the complete nucleotide sequences of CLBV isolates infecting citrus in China.

关键词： Citrus leaf blotch virus (CLBV) genome sequence Citrivirus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development and pathogenicity analysis of full-length infectious cDNA clones of citrus yellow mottle-associated virus in citrus plants

引用

Journal of Integrative Agriculture 2023年第10期22卷 3034-3041页

作者： WU jia-xing ZHANG Song LIANG Xiao-fei XING Fei Sagheer ATTA WANG Xue-feng cao meng-ji National Citrus Engineering and Technology Research Center Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science CityCitrus Research InstituteSouthwest UniversityChongqing 400712P.R.China Guangxi Citrus Breeding and Cultivation Technology Innovation Center Guangxi Academy of Specialty CropsGuilin 541004P.R.China Department of Plant Protection Faculty of Agricultural SciencesGhazi UniversityDera Ghazi Khan 32200Pakistan

Citrus yellow mottle-associated virus(CiYMaV)belonging to the subgenus Mandarivirus within the genus Potexvirus,was first identified in 2018 from Pakistan(CiYMaV-PK),where it is endemic in several ***,three full-length cDNA clones(pCiYMaV-FL-1,pCiYMaV-FL-18,and pCiYMaV-FL-22)corresponding to the genomic RNA of CiYMaV were constructed and then agroinfiltrated on Chandler pummelo(Citrus grandis)seedlings using the vacuum infiltration *** the inoculated plants developed severe vein yellowing,leaf mottling,and dwarfing symptoms by 40 days post-infiltration(dpi).The results of a direct tissue blot immunoassay and reverse transcription polymerase chain reaction detection showed 94.7–100%infection rates of pCiYMaV-FL at 60 *** there being no observed difference among the three clones in the severity of symptom,pCiYMaV-FL-22 showed the highest accumulation levels of viral RNA and coat ***,pCiYMaV-FL-22 successfully infected seven other citrus varieties and induced symptoms in five of *** electron microscopy identified the presence of filamentous virus particles in extracts from systemic leaves of the plants infected with pCiYMaV-FL-22 at 6-months *** together,the results indicate that Koch's postulates were fulfilled for CiYMaV in citrus *** is the first report of full-length infectious cDNA clones of CiYMaV,and thus,the data provide a basis for further study of the molecular mechanisms of virus infection and the development of a viral vector to express foreign genes in citrus plants.

关键词： Mandarivirus vein yellow vacuum infiltration direct tissue blot immunoassay virus morphology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The complete genome sequence of Citrus vein enation virus from China

引用

Journal of Integrative Agriculture 2015年第3期14卷 598-601页

作者： HUANG Ai-jun SONG Zhen cao meng-ji CHEN Hong-ming LI Zhong-an ZHOU Chang-yong Citrus Research Institute Chinese Academy of Agricultural Sciences Southwest University College of Plant Protection Southwest University

The complete nucleotide sequence of an isolate of Citrus vein enation virus（CVEV-XZG） from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames（ORFs）, had a similar genomic organization features with Pea enation mosaic virus（PEMV）. Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.

关键词： Citrus vein enation virus(CVEV) genome sequence Enamovirus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Apple stem grooving virus is associated with leaf yellow mottle mosaic disease on Citrus grandis cv. Huangjinmiyou in China

引用

Journal of Integrative Agriculture 2022年第7期21卷 2031-2041页

作者： XUAN Zhi-you ZHANG Song LI Ping YANG Fang-yun CHEN Hong-ming LIU Ke-hong ZHOU Yan LI Zhong-an ZHOU Chang-yong cao meng-ji National Citrus Engineering and Technology Research Center Citrus Research InstituteSouthwest UniversityChongqing 400712P.R.China State Cultivation Base of Crop Stress Biology for Southern Mountainous Land Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China

Although it is usually latent on citrus, apple, and pear, apple stem grooving virus(ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic(LYMM) symptoms have been observed on Huangjinmiyou(HJY) pummelos(Citrus grandis cv. Huangjinmiyou), a commercial variety that is widely cultivated in South China, high throughput sequencing(HTS) was used to find potential pathogens and only three divergent ASGV variants were identified. The three ASGV variants shared 81.03–82.34% genome-wide pairwise identities with each other, and were separately closest to other ASGV variants from different hosts and/or geographical regions, as indicated by viral phylogenies. However, these new variants may have developed from viral interstrain interactions, based on the results of recombination analysis. A large-scale survey using reverse transcription-PCR(RT-PCR) protocols designed for the three ASGV variants revealed a high incidence(92.7–100%) of ASGV in symptomatic HJY trees from 11 major citrusproducing regions in China. None of ASGV were detected in asymptomatic trees. Temperature treatments applied to the symptomatic HJY plants showed that ASGV is sensitive to high temperatures(30–35°C), at which not only the plants recovered, but also the viruses were not detected by RT-PCR, while at low temperatures(20–24°C), both the symptoms and viruses remained detectable. These data show that ASGV is associated with the LYMM disease prevalent on HJY in China, and this is the significant basis especially of taking appropriate measures timely to manage the disease.

关键词： citrus pummelo apple stem grooving virus genome sequence thermotherapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Applications of Next Generation Sequencing in Plant Virology

Applications of Next Generation Sequencing in Plant Virology

引用

中国植物病理学会2016年学术年会

作者： cao meng-ji ZHOU Chang-yong LI Ru-hui DING Shou-wei National Citrus Engineering Research Center Citrus Research InstituteSouthwest University/Chinese Academy of Agricultural Sciences USDA-ARS National Germplasm Resources Laboratory Department of Plant Pathology and Microbiology University of California

Nextgeneration sequencing（NGS） technologies provide a high throughput,efficient andfast DNAsequencing platform compared to the standard and traditional *** technologies have been rapidly applied to several areas of plant virology including virus/viroid genome sequencing,discovery and detection,ecology,replication and antiviral mechanism since *** report here the use of small interfering RNAs（siRNAs） and Hiseq RNA sequencing for the study of virus-host interaction and virus detection/*** of the siRNA sequencing data revealed the presence of a genetically distinct class of virus-activated siRNAs（vasiRNAs） in Arabidopsis *** propose that antiviral RNAi activates broad-spectrum antiviral activity via widespread silencing of host genes directed by vasiRNAs in addition to specific antiviral defense by viral *** siRNA and Hiseq RNA sequences were used to identify known and unknown pathogens from citrus,fruit trees and sweet *** results showed that fast,accurate,and full indexing and identification of the pathogens were achieved by both *** is expected that NGS will play veryprominent roles in fundamental and applied research areas of plant virology.

关键词： Next generation sequencing Virus-activated siRNAs Virus identification

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Theoretical Study of O2 Molecular Adsorption and Dissociation on Silica Carbide Nanotubes

Theoretical Study of O2 Molecular Adsorption and Dissociatio...

引用

第十届全国计算(机)化学学术会议

作者： Xianyan Xu,Fenglei cao,Yue-meng ji and Cunyuan Zhao~* School of Chemistry and Chemical Engineering Sun Yat-sen University,Guangzhou 510275,

The adsorption/dissociation of O molecule on the surface of SiCNTs was investigated by density functional *** found several adsorption configurations,including chemisorption and cycloaddition configurations,for triplet and singlet *** the case of carbon nanotubes,the chemisorption of triplet O on silica carbide nanotubes is exothermic with remarkable charge transfer from nanotubes to O *** O adsorption on the surface of SiCNTs can yields cycloaddition structures with large binding energies and sizeable charge *** reaction mechanisms studies show that for triplet O chemisorption configuration is favorable,but cycloaddition configuration is preferred than chemisorption for singlet *** singlet O,we also studied the dissociation of O molecule,and a two-step mechanism was found. In dissociation produces,the dissociation of O molecular result in formation of two three-membered rings with large binding *** key reaction step in the dissociation reaction of singlet O on the surface of SiCNTs is the first step with the barrier energy of 0.40 eV. The electronic properties of SiCNTs adsorbed with triplet and singlet O are dramatically influence.

关键词： O2 molecule SiCNTs adsorption dissociation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

高通量测序发现河南开封、中牟西瓜病毒病由多种病毒复合侵染导致

引用

植物病理学报 2020年第3期50卷 286-291页

作者：姜军吴楠辛敏曹孟籍王锡锋河南省开封市祥符区农业科学研究所开封475100 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193 西南大学柑橘研究所国家柑橘工程技术研究中心重庆400712

调查发现河南省开封市祥符区和郑州市中牟县西瓜田病毒病严重发生,症状表现为叶片黄化、蕨叶、皱缩、卷曲,枝梢翘起,果实变小和瓜纹不清。为明确其病毒种类,通过小RNA测序及生物信息学分析发现采集的西瓜样本中存在8种病毒。其中包括5... 详细信息

调查发现河南省开封市祥符区和郑州市中牟县西瓜田病毒病严重发生,症状表现为叶片黄化、蕨叶、皱缩、卷曲,枝梢翘起,果实变小和瓜纹不清。为明确其病毒种类,通过小RNA测序及生物信息学分析发现采集的西瓜样本中存在8种病毒。其中包括5种已知病毒:小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)、甜瓜蚜传黄化病毒(Melon aphid-borne yellows virus,MABYV)、西瓜花叶病毒(Watermelon mosaic virus,WMV)、黄瓜绿斑驳花叶病毒(Cucumber green mottle mo-saic virus,CGMMV)和西瓜隐潜病毒(Citrullus lanatus cryptic virus,CiLCV);3种为新检出的RNA病毒:西瓜病毒A(Watermelon virus A,WVA)、西瓜皱叶相关病毒1号(Watermelon crinkle leaf-associated virus 1,WCLaV-1)和西瓜皱叶相关病毒2号(Watermelon crinkle leaf-associated virus 2,WCLaV-2)。RT-PCR检测田间66个西瓜标样发现,WMV、CiLCV和ZYMV是侵染两地西瓜的主要病毒,其阳性检出率分别为61%、56%和52%,两种及以上病毒复合侵染的比率达68%。

关键词：西瓜小RNA测序病毒病复合侵染

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：