Apple stem grooving virus is associated with leaf yellow mottle mosaic disease on Citrus grandis cv. Huangjinmiyou in China

作     者：XUAN Zhi-you ZHANG Song LI Ping YANG Fang-yun CHEN Hong-ming LIU Ke-hong ZHOU Yan LI Zhong-an ZHOU Chang-yong CAO Meng-ji XUAN Zhi-you;ZHANG Song;LI Ping;YANG Fang-yun;CHEN Hong-ming;LIU Ke-hong;ZHOU Yan;LI Zhong-an;ZHOU Chang-yong;CAO Meng-ji

作者机构：National Citrus Engineering and Technology Research CenterCitrus Research InstituteSouthwest UniversityChongqing 400712P.R.China State Cultivation Base of Crop Stress Biology for Southern Mountainous LandAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第7期

页      面：2031-2041页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 

基　　金：supported by the National Key R&D Program of China(2019YFD1001800) the National Natural Science Foundation of China(32072389) the Innovation Program for Chongqing’s Overseas Returnees(cx2019013) 111 Project(B18044)from Ministry of Education(China) 

主　　题：citrus pummelo apple stem grooving virus genome sequence thermotherapy 

摘      要：Although it is usually latent on citrus, apple, and pear, apple stem grooving virus(ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic(LYMM) symptoms have been observed on Huangjinmiyou(HJY) pummelos(Citrus grandis cv. Huangjinmiyou), a commercial variety that is widely cultivated in South China, high throughput sequencing(HTS) was used to find potential pathogens and only three divergent ASGV variants were identified. The three ASGV variants shared 81.03–82.34% genome-wide pairwise identities with each other, and were separately closest to other ASGV variants from different hosts and/or geographical regions, as indicated by viral phylogenies. However, these new variants may have developed from viral interstrain interactions, based on the results of recombination analysis. A large-scale survey using reverse transcription-PCR(RT-PCR) protocols designed for the three ASGV variants revealed a high incidence(92.7–100%) of ASGV in symptomatic HJY trees from 11 major citrusproducing regions in China. None of ASGV were detected in asymptomatic trees. Temperature treatments applied to the symptomatic HJY plants showed that ASGV is sensitive to high temperatures(30–35°C), at which not only the plants recovered, but also the viruses were not detected by RT-PCR, while at low temperatures(20–24°C), both the symptoms and viruses remained detectable. These data show that ASGV is associated with the LYMM disease prevalent on HJY in China, and this is the significant basis especially of taking appropriate measures timely to manage the disease.