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Influence of local and landscape environmental factors on alpha and beta diversity of macroinvertebrates in Andean rivers

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Journal of Mountain Science 2023年第9期20卷 2487-2501页

作者： DIAZ-ROJAS camila A PEDROZA-ramos Adriana X BARRERA-HERRERA July A ROA-FUENTES Camilo A Ecology Unit in Aquatic Systems Research Group Pedagogical and Technological University of ColombiaUPTCAvenida Central del Norte 39-115TunjaBoyacáColombia

Research on macroinvertebrate community structure in Andean rivers has been oriented towards describing patterns of alpha and gamma diversity by taking into account environmental predictors at local spatial scales(e.g.,micro-and mesohabitats).However,the patterns of beta diversity and the importance of landscape-scale variables have been evaluated to a lesser *** objective of this study was to describe the patterns of alpha and beta diversity of benthic macroinvertebrates in the Andean rivers of the Orinoco basin and their relationship with local and landscape environmental variation.A stratified random sampling of macroinvertebrates was carried out at 40 sites(comprising an altitudinal range of between 500 and 2900 m.a.s.l.),local and landscape variables were *** results showed that the variation of alpha diversity was influenced by local and landscape variables,which are directly and indirectly related to the contribution of sediments,substrate composition and flow velocity,providing a heterogeneity of *** beta diversity was explained by the combined effect of local and landscape *** the beta diversity phenomena,turnover was predominant while nestedness presented a minor contribution and both were explained in greater proportion by local descriptors and some landscape variables,specifically those of a geomorphological *** results concur with the view of an environmental and spatial hierarchy within the river habitat and highlight the influence of multiple scales on macroinvertebrate *** above suggests that both local and landscape scales must necessarily be considered for environmental management and the conservation of Andean lotic ecosystems.

关键词： Andean lotic systems Andean agroecosystems Instream habitat Landcover Turnover Nestedness

Teleophthalmology: a strategy for timely diagnosis of sight-threatening diabetic retinopathy in primary care, Concepción, Chile

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International Journal of Ophthalmology(English edition) 2019年第9期12卷 1474-1478页

作者： Angelica Avendano-Veloso Felipe Parada-Hernandez Raul Gonzalez-ramos camila Dougnac-Osses JoseLuis Carrasco-Saez Peter Henry Scanlon Telemedicine Unit University of ConcepcionConcepcion4070386Chile School of Medicine University of ConcepcionConcepcion4070386Chile University of Oxford University of GloucestershireOxfordOX1 2JDEnglandUK

AIM: To estimate the prevalence of diabetic retinopathy(DR) in a diabetic population of the public health system. METHODS: This non-experimental, descriptive and crosssectional study, with no direct control over the variables analysed, was carried out by the Telemedicine Unit of the University of Concepción(TELMED) and the Family Health Centers(CESFAM) of the Health Service Concepción, Chile, among a population of 7382 diabetic patients of the public health system, from October 2014 to June 2015. Two digital images for each patient’s eyes were obtained and uploaded to the TELMED tele-ophthalmology platform. The images were remotely evaluated by a retina expert ophthalmologist for diagnosis. Treatment and a referral(if required) were recommended, and an individualised report for each patient was sent to the place of ***: The prevalence of DR in this study was 14.9%. Of these, 48.7% had mild non-proliferative DR(NPDR), 30.8% moderate NPDR, 15.9% severe NPDR, and 4.6% proliferative DR. Of the patients with DR, 17.8% were referred for fluorescein angiography, 4.3% for panretinal photocoagulation, 1.2% for vitrectomy, and 0.4% for cataract ***: The use of telemedicine allowed an increased screening coverage for DR in diabetic patients. The different types of DR were detected in a timely manner, decreasing waiting times due to the lack of specialists in ophthalmology in the public health system.

关键词： ophthalmology teleophthalmology diabetes mellitus diabetic retinopathy telemedicine

Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

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Journal of Animal Science and Biotechnology 2017年第3期8卷 574-581页

作者： Daniel S.ramos Angrimani Marcilio Nichi Joāo Diego A.Losano Cristina F.Lucio Gisele A.Lima Veiga Márcia V.M.Junqueira Franco camila I.Vannucchi Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of Sāo Paulo Rua Prof. Orlando Marques de Paiva 87 Sāo Paulo 05508-270 Brazil Department of Internal Medicine Division of Nutrition University of Sāo Paulo Av. Bandeirantes 3900 - 14040-040 Ribeirāo Preto. Brazil

Background： During sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis（lipids were extracted, transmethylated and analyzed by chromatography）.Results： Caput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity, indicating different levels of the fatty acids organization. Saturated, monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda. Highlighting the presence of caprylic, stearic and docosahexaenoic ***： These findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization.

关键词： Dogs Epididymis Fatty acids Sperm maturation

Quantification of the Expression of HIF-1alpha by Real-Time PCR in Rat Hepatocytes Cultures Invaded by <i>Shigella flexneri</i>under Normoxic and Hypoxic Conditions

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Advances in Microbiology 2015年第7期5卷 507-516页

作者： camila Bárbara Cantalupo Lima Sania Alves dos Santos Dahir ramos de Andrade Júnior Laboratory of Bacteriology (LIM 54) Department of Infectious and Parasitic Diseases Faculty of Medicine University of Sao Paulo Sao Paulo Brazil

Aim: Shigella flexneri (S. flexneri) is a gram-negative enterobacterium responsible for severe intestinal end systemic infection in humans. The bacteria can reach the liver due to degeneration of the colonic epithelium. Hypoxia is present in many human diseases and can induce the expression of the transcription factor HIF-1alpha that may have a cell protective role. The influence of hypoxia and HIF-1alpha on bacterial infection, studied in this work, is unclear. Hypoxia inducible factor-1alpha (HIF-1alpha) is a transcription factor that acts as a master regulator of gene expression induced by hypoxia. Methods: We compared the ability of S. flexneri to invade rat hepatocytes in primary culture both in normoxic and hypoxic conditions. We evaluated TNF-alpha released by hepatocytes, apoptosis rate and HIF-1alpha expression by confocal microscopy as well as real time PCR technique. Results: We showed that S. flexneri invaded less hepatocytes previously submitted to 24 h hypoxia (6.5% O2) than those cultivated in normoxia (21% O2). S. flexneri also induced HIF-1α expression in hepatocytes, TNF-α secretion and apoptosis. Conclusion: a) Hypoxia alone was not a stimulus to TNF-α secretion, but induced cell apoptosis and HIF-1α expression;b) S. flexneri was able to invade rat hepatocytes and hypoxia apparently influenced significantly bacterial cell invasiveness;c) HIF-1α was expressed in hypoxic conditions, and it was also stimulated by S. flexneri.

关键词： Shigella flexneri Hepatocytes Cell Hypoxia Hypoxia-Inducible Factor 1α TNF-Alpha Apoptosis

Antioxidant Action of Rosemary and Oregano Extract in Pre-Cooked Meat Hamburger

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Food and Nutrition Sciences 2018年第7期9卷 806-817页

作者： Maria Raquel Manhani Maria Aparecida Nicoletti Andrea Carla Da Silva Barretto Graciele Riveres De Jesus camila Munhoz Gabriel ramos De Abreu Julia Zaccarelli-Magalhaes Andre Rinaldi Fukushima Instituto Federal de Sã o Paulo(IFSP) Campus SuzanoSuzanoBrazil Faculty of Pharmaceutical Sciences University of Sao PauloSao PauloBrazil Instituto de Biociencias Letras e Ciências Exatas de Sao Jose do Rio Preto Universidade Estadual Paulista Júlio de Mesquita FilhoSao Josédo Rio PretoBrazil Universidade Sao Judas Tadeu Sao PauloBrasil Department of Pathology School of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao PauloBrazil Health Science Institute Presbiterian Mackenzie UniversitySao PauloBrazil

The main goal is to compare the antioxidant potential of rosemary and oregano natural extracts in precooked beef burger by assessing the lipid oxidation extent and sensory analysis. Five formulations (F) of hamburger were prepared from beef and mechanically separated as follows: meat containing sodium erythorbate (F1);deodorized rosemary extract (F2);oregano extract (F3);rosemary plus oregano extracts (F4) and without antioxidant addition, denominated control formulation (CF). The samples were frozen at ?18°C for 24 hours, then submitted to heat treatment in an electric oven with internal controlled temperature of 75°C, and again frozen for a period of 30 days. The lipid oxidation extent (determined by thiobarbituric acid reactive substances— TBRS) was evaluated at 0, 15 during 30 days. After heating in plate to temperature of 75°C, the samples were submitted to color, taste and odor evaluation by 40 untrained tasters. The formulations F1, F2 and F4 presented lower concentrations of TBRS, whereas CF at day zero already showed very high values, indicating oxidation of the product. The samples showed good acceptance in the sensorial analysis.

关键词： Rosemary Extract Oregano Extract Food Composition Food Preservation Food Quality Meat Product

Knowledge of Female Prisoners in a City of Ceará, Brazil, about Sexually Transmitted Diseases

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Open Journal of Nursing 2014年第9期4卷 630-636页

作者： Cinthia Gondim Pereira Calou camila Fonseca Wasidi Maria de Fátima Esmeraldo ramos de Figueiredo Ana Izabel Oliveira Nicolau Ana Karina Bezerra Pinheiro Régia Christina Moura Barbosa Castro Priscila de Souza Aquino Universidade Federal do Ceará Fortaleza Brazil Universidade Federal do Ceará Fortaleza Brazil Universidade Regional do Cariri Crato Brazil Universidade Regional do Cariri Crato Brazil

Exploratory study was conducted in the Crato Public Prison, State of Ceará, Brazil, aiming to understand the knowledge and experiences related to the preventive aspects of Sexually Transmitted Diseases (STDs) in the prison context. The target population of the study consisted of 14 female inmates, 10 of whom agreed to participate. Data collection occurred in June 2012 through recorded semi-structured individual interviews. We investigated quantitative, socio-demographic and sexual variables. In qualitative data analysis, we applied the Meaning Interpretation Method. The female vulnerability was aggravated by the early onset of sexual activity, lack of knowledge about STDs, non-adoption of preventive measures and history of prostitution. The following categories emerged from the qualitative assessment: Knowledge about STD/AIDS versus Personal Experience;Use of contraception as STD/AIDS prevention within the Public Jail;Habits of inmates: alcohol and tobacco;and Characterization of the conjugal visit area.

关键词： Prisoners Nursing Women’s Health Sexual and Reproductive Health

Determination of Paraquat in Several Commercially Available Types of Rice

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Food and Nutrition Sciences 2018年第12期9卷 1368-1375页

作者： Thais Lopes Lima Maria Aparecida Nicoletti camila Munhoz Gabriel ramos De Abreu Julia Zaccarelli Magalhaes Esther Lopes Ricci Ricci Paula A.Faria Waziry Júlia Nathalia Alves da Costa Ana Carolina Nascimento Antonio AndréRinaldi Fukushima Universidade Sao Judas Tadeu-R.Taquari Sao Paulo/SPBrazil Departamento de Farmacia.Faculdade de Ciencias Farmaceuticas.Universidade de Sao Paulo Sao Paulo/SPBrazil Instituto de Ciencias da Saude Universidade Presbiteriana MackenzieRua da ConsolacaoSao Paulo/SPBrazil Departamento de Patologia Faculdade de Medicina Veterinaria e ZootecniaUniversidade de Sao PauloSao Paulo/SPBrazil New Southeastern University Fort LauderdaleFloridaUnited States of America Veterans Affairs Medical CenterMiamiFloridaUSA Faculdade Sao Bento-Largo de Sao Bento s/no-CentroSao Paulo-SPSao Paulo/SPBrazil

Introduction: Paraquat (1,1’-dimethyl-4,4’-bipyridine-dichloride) is a herbicide used to combat weeds and improve crop productivity. Its acute toxicity can cause fatal poisoning in humans and animals and several studies have shown a strong correlation between paraquat and Parkinson’s disease. Objective: To determine the concentration of paraquat in different types of rice in Brazil. Method: Paraquat quantification is based on a complexation reaction with sodium dithionite, which generates a blueish color compound, whose absorbance was read in a spectrophotometer at the 600 nm wavelength. Results: Five samples were found to be above the maximum allowed limit, while one sample had a concentration in the upper allowed limit. The method employed did not allow quantification of black rice. Conclusion: There is a need for identification of the type of pesticide used in each examined crop as well as their respective dates of remission in order to improve the safety of agricultural practices.

关键词： Paraquat Herbicide Rice

Acting Front to Nursing Psychological Repercussions in Breast Cancer Patients

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Open Journal of Nursing 2016年第12期6卷 987-994页

作者： Maria do Socorro Santos de Oliveira Adalberto Cruz Sampaio camila Macêdo de Figueiredo Silva Morais de Santana Ferreira Natália Moreira Garcia Feitosa Rosângela Rodrigues dos Santos Petrúcya Frazão Lira Milana Drumond ramos Santana College of Juazeiro do Norte Juazeiro do Norte CE Brazil University Center Leão Sampaio Juazeiro do Norte CE Brazil Retired Juazeiro do Norte CE Brazil Maternity Hospital São Vicente College of Medicine Juazeiro do Norte CE Brazil Center for Attention to Student (NAE) College of Juazeiro do Norte Juazeiro do Norte CE Brazil

Breast cancer is a heterogeneous and complex disease with different situations of threat to their patients, which can cause psychological discomfort, anxiety and a depressed state in women. The aim of this study was to analyze the performance of nursing to psychological repercussions on living with breast cancer. This is a descriptive and cross-sectional study with qualitative characteristics. It was conducted with professional nurses in Basic Health Units (UBS) in the municipality of Juazeiro do Norte-CE, Northeast of Brazil, located in the region of Cariri. The study began to be developed after the approval of the Research Ethics Committee, CAAE: 50809315.8. 0000.5624. Data collection occurred through a semi-structured interview. For the interpretation of the results, the technique of content analysis was used. The results showed that nurses realize that emotional support, care and assistance in coping, improve the construction of a good performance of the professional nurse. However, it is possible to realize the need for information and qualified assistance by multidisciplinary team to these patients and even their family members. It was identified as difficulty in assisting the denial of patient to start the treatment, but the facility is in the intimacy that the professional nurse can engage with the customer. It highlighted the care through dialogue and health education. And, finally, the nurse is aware of such importance of his/her role when supporting the patient. Thus, the improvement of professional assistance was evidenced as regards emotional support, reception, qualified listening, health education measures leading to self-knowledge, self-esteem and acceptance of the disease and the creation of bonds with the patients.

关键词： Nursing Care Breast Cancer Women’s Health

Analysis of Nursing Care Concept: Perspective of the Person Submitted to Care

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Open Journal of Nursing 2016年第6期6卷 491-496页

作者： camila Brasil Moreira Ana Fátima Carvalho Fernandes Míria Conceição Lavinas Santos Altamira Mendonça Félix Gomes Carla Monique Lopes Mourão Rosy Denyse Pinheiro de Oliveira Angelica Isabely de Morais Almeida Suzy ramos Rocha Cláudia Rayanna Silva Mendes Sadrine Maria Eufrasino de Pinho Marinna Maria de Andrade Costa Jordana Prado Benevides Caroline Batista de Queiroz Aquino Iarlla Silva Ferreira Raquel Vilanova Araujo Department of Nursing Federal University of Ceará Fortaleza Brazil

This study aimed to analyze the concept of nursing care to patients with breast cancer on chemotherapy treatment, according to the perspective of the person submitted to care. The study followed the conceptual analysis methodology proposed by Walker and Avant according to Waldow, and it was developed through consultation to SCOPUS and CINAHL databases. The selection was carried out by inclusion and exclusion criteria, constituting a sample of 7 articles. There have been identified the main uses of the risk concept, defining attributes, antecedents and consequences of the concept. The evaluation of the concept and nursing care to breast cancer patient on chemotherapy treatment allowed a broader understanding of the phenomenon, with emphasis on the skills and competencies in nursing care dispensed to woman on chemotherapy treatment for breast cancer.

关键词： Nursing Breast Neoplasms Analysis Research Nursing Care Chemotherapy