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Suppression of bcl-2 Gene by RNA Interference Increases Chemosensitivity to Cisplatin in Nasopharyngeal Carcinoma Cell Line CNE1

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 749-753页

作者： Zhi-Hua YIN, cai-ping REN, feng LI, Xu-Yu YANG, Hui LI, Ming ZHAO, and Kai-Tai YAOCancer Research Institute, Central South University, Changsha 410078, China Cancer Research Institute Central South University

To explore the effect of suppressing BCL-2 expression using RNA interference (RNAi) technique in nasopharyngeal carcinoma cell line CNE1. CNE1 cell lines stably expressing shRNAs targeted bcl-2 and GL3 gene were established and gene expression inhibition was assessed by Western blotting analysis. The effect of suppressing bcl-2 by RNAi on cell growth was studied, the apoptosis induction and the sensitization of CNE1 cells to cisplatin were quantified by MTT assay and flow cytometry. The results showed that: stable transfection of CNE1

关键词： RNA interference nasopharyngeal carcinoma bcl-2 cisplatin

微生物实验室参加FEPAS能力验证计划的质量控制及对策

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食品科技 2011年第7期36卷 256-260页

作者：王立平蔡雪凤国家食品质量安全监督检验中心北京100094

目的:通过对微生物实验室参加FEPAS比对能力验证计划实施及结果分析,了解国际间微生物检测整体水平,探讨提高能力验证工作有效控制方法和措施。方法:采用传统生理生化鉴定方法和VITEK全自动微生物鉴定系统相结合的办法,充分考虑检测过... 详细信息

目的:通过对微生物实验室参加FEPAS比对能力验证计划实施及结果分析,了解国际间微生物检测整体水平,探讨提高能力验证工作有效控制方法和措施。方法:采用传统生理生化鉴定方法和VITEK全自动微生物鉴定系统相结合的办法,充分考虑检测过程中可能影响结果的因素,对目标微生物进行准确的定量或定性。结果:所采用的方法能准确检测样品中的目标菌,8项FEPAS比对能力验证计划均获得满意成绩。结论:FEPAS比对能力验证计划结果反应了各参加实验室微生物检测能力和水平。积极参与FEPAS比对能力验证计划对提高微生物实验室整体检测水平的提升具有重要的促进作用。

关键词： FEPAS 微生物定性定量质量控制

Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats

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World Journal of Gastroenterology 2025年第1期31卷 93-109页

作者： ping Zhang Ying Zhu Pu Chen Tong Zhou Zhe-Yi Han Jun Xiao Jian-feng Ma Wen Ma Peng Zang Ying Chen Graduate School Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Key Laboratory of Aerospace Nutrition and Food Engineering China Astronaut Research and Training CenterBeijing 100094China Department of Gastroenterology The Air Force Medical CenterBeijing 100142China Department of Blood Transfusion Air Force Medical CenterBeijing 100142China

BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility *** pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal ***,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal *** cells are the pacemakers of gastrointestinal *** To investigate the effects of Bifidobacterium lactis(***)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit *** The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 *** tail group was given *** BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/***&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and *** fecal flora and mucosal flora of rats in each group were detected by 16S *** Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic *** microgravity reduced the expressions of SCF and c-kit,and increased the express

关键词： Simulated microgravity Rat Intestinal flora Gastrointestinal motility Stem cell factor/c-kit signaling pathway

绍兴地区手足口病门诊患儿病原学及其T细胞亚群及IL-13变化

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中华医院感染学杂志 2021年第1期31卷 156-160页

作者：喻立娣张彬冯雅琴顾英蔡凤萍章建伟绍兴市妇幼保健院健康教育科浙江绍兴312000 绍兴市妇幼保健院产科浙江绍兴312000 绍兴市妇幼保健院发热门诊浙江绍兴312000 绍兴市妇幼保健院儿内科浙江绍兴312000 绍兴市妇幼保健院妇科浙江绍兴312000 绍兴市妇幼保健院儿保科浙江绍兴312000

目的分析绍兴地区手足口病(hand foot and mouth disease,HFMD)门诊患儿病原学特点及其与T淋巴细胞亚群、白细胞介素-13(Interleukin-13,IL-13)水平的关系。方法选择2017-2019年绍兴市妇幼保健院收治的1323例HFMD门诊患儿为研究对象,采... 详细信息

目的分析绍兴地区手足口病(hand foot and mouth disease,HFMD)门诊患儿病原学特点及其与T淋巴细胞亚群、白细胞介素-13(Interleukin-13,IL-13)水平的关系。方法选择2017-2019年绍兴市妇幼保健院收治的1323例HFMD门诊患儿为研究对象,采用流行病学方法对病例及标本检测情况进行描述性分析,并比较不同类型病毒感染患儿T淋巴细胞亚群、IL-13水平变化。结果1323例HFMD门诊患儿检出病毒阳性1227例(92.74%),其中肠道病毒71型(Enterovirus 71,EV71)感染749例(61.04%)、柯萨奇A组16型(Coxsachie virus,Cox A16)感染355例(28.93%)、其他肠道病毒感染123例(10.02%);1227例患儿年龄1~5岁占比最高为59.41%、男性占59.49%、发病时间4~6月占47.76%、散居患儿占58.60%;危重症、重症、普通占比分别为0.81%、21.11%、78.08%;三组患儿病情严重程度比较差异显著(Z=30.540,P<0.001);Cox A16感染、其他肠道病毒感染、EV71感染患儿T淋巴细胞CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)水平逐渐降低,CD_(8)^(+)、IL-13水平逐渐升高,三组比较差异有统计学意义(P<0.05)。结论HFMD患儿以EV71感染为主,Cox A16次之,在4~6月发病率最高,好发于1~5岁儿童,散居儿童为主,EV71重症患儿占比较高,EV71引起的免疫紊乱、炎性反应高于其他病毒。

关键词：手足口病病原学 T淋巴细胞亚群白细胞介素-13

Prognostic model for esophagogastric variceal rebleeding after endoscopic treatment in liver cirrhosis: A Chinese multicenter study

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World Journal of Gastroenterology 2025年第2期31卷 85-101页

作者： Jun-Yi Zhan Jie Chen Jin-Zhong Yu Fei-Peng Xu Fei-Fei Xing De-Xin Wang Ming-Yan Yang feng Xing Jian Wang Yong-ping Mu Cell Biology Laboratory Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Institute of Liver Diseases Shanghai Academy of Chinese MedicineShanghai 201203China Clinical Key Laboratory of Traditional Chinese Medicine of Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Key Laboratory of Liver and Kidney Disease of the Ministry of Education Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Gastroenterology and Hepatology Zhongshan Hospital Affiliated to Fudan UniversityShanghai 200032China Shanghai Institute of Liver Disease Fudan UniversityShanghai 200032China Evidence-Based Medicine Center Fudan UniversityShanghai 200032China Department of Gastrointestinal Endoscopy Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Hepatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China

BACKGROUND Rebleeding after recovery from esophagogastric variceal bleeding(EGVB)is a severe complication that is associated with high rates of both incidence and *** its clinical importance,recognized prognostic models that can effectively predict esophagogastric variceal rebleeding in patients with liver cirrhosis are *** To construct and externally validate a reliable prognostic model for predicting the occurrence of esophagogastric variceal *** This study included 477 EGVB patients across 2 cohorts:The derivation cohort(n=322)and the validation cohort(n=155).The primary outcome was rebleeding events within 1 *** least absolute shrinkage and selection operator was applied for predictor selection,and multivariate Cox regression analysis was used to construct the prognostic *** validation was performed with bootstrap *** assessed the discrimination,calibration and accuracy of the model,and performed patient risk *** Six predictors,including albumin and aspartate aminotransferase concentrations,white blood cell count,and the presence of ascites,portal vein thrombosis,and bleeding signs,were selected for the rebleeding event prediction following endoscopic treatment(REPET)*** predicting rebleeding within 1 year,the REPET model ex-hibited a concordance index of 0.775 and a Brier score of 0.143 in the derivation cohort,alongside 0.862 and 0.127 in the validation ***,the REPET model revealed a significant difference in rebleeding rates(P<0.01)between low-risk patients and intermediate-to high-risk patients in both *** We constructed and validated a new prognostic model for variceal rebleeding with excellent predictive per-formance,which will improve the clinical management of rebleeding in EGVB patients.

关键词： Esophagogastric variceal bleeding Variceal rebleeding Liver cirrhosis Prognostic model Risk stratification Secondary prophylaxis

L^q Interior Estimates of the Gradient for Weak Solutions of Quasilinear Elliptic Equations

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Acta Mathematica Sinica,English Series 2007年第12期23卷 2225-2234页

作者： feng ping YAO Yong Yong cai LMAM School of Mathematical Sciences Peking University Bering 100871 P. R. China

The authors present a new proof of L^q interior estimates of the gradient for weak solutions of a quasilinear elliptic equation of p-Laplacian type.

关键词： L^q interior estimates weak solutions quasilinear elliptic maximal function

冠状动脉粥样硬化、急性脑梗死患者血栓弹力图血小板图参数、血小板凝集功能及其之间的关系

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实用心脑肺血管病杂志 2025年第1期33卷 44-49页

作者：李师亮冯异程才郑萍华中科技大学同济医学院附属同济医院心脏大血管外科湖北省武汉市430000

目的分析冠状动脉粥样硬化(CAA)、急性脑梗死(ACI)患者血栓弹力图血小板图(TEG-PM)参数、血小板凝集功能情况及其之间的关系。方法选取2020-01-01至2022-01-01华中科技大学同济医学院附属同济医院收治的CAA患者73例为CAA组及ACI患者66例... 详细信息

目的分析冠状动脉粥样硬化(CAA)、急性脑梗死(ACI)患者血栓弹力图血小板图(TEG-PM)参数、血小板凝集功能情况及其之间的关系。方法选取2020-01-01至2022-01-01华中科技大学同济医学院附属同济医院收治的CAA患者73例为CAA组及ACI患者66例为ACI组。选取同期在华中科技大学同济医学院附属同济医院体检中心进行健康体检者52例为对照组。根据疾病严重程度,将CAA组患者进一步分为Ⅰ级亚组(冠状动脉无明显狭窄,n=20)、Ⅱ级亚组(冠状动脉狭窄率为40%~69%,n=19)、Ⅲ级亚组(冠状动脉狭窄率为70%~99%,n=20)、Ⅳ级亚组(冠状动脉完全闭塞,n=14),将ACI组患者进一步分为轻度亚组[美国国立卫生研究院卒中量表(NIHSS)评分为1~4分,n=35]、中度亚组(NIHSS评分为5~15分,n=15)、重度亚组(NIHSS评分≥16分,n=16)。比较CAA组与对照组、ACI组与对照组及不同疾病严重程度CAA、ACI患者TEG-PM参数[凝血反应时间(R)、血细胞凝集块形成时间(K)、血细胞凝集块形成速率(α角)、最大振幅(MA)、花生四烯酸(AA)途径抑制率、二磷酸腺苷(ADP)途径抑制率]、血小板凝集率。采用Pearson相关分析探讨CAA、ACI患者TEG-PM参数与血小板凝集率的相关性。结果CAA组、ACI组R、K短于对照组,α角、MA大于对照组,AA途径抑制率、ADP途径抑制率低于对照组,血小板凝集率高于对照组(P<0.05)。Pearson相关分析结果显示,CAA组、ACI组R、K、AA途径抑制率、ADP途径抑制率均与血小板凝集率呈负相关,α角、MA均与血小板凝集率呈正相关(P<0.05)。Ⅱ级亚组、Ⅲ级亚组、Ⅳ级亚组R短于Ⅰ级亚组,Ⅲ级亚组、Ⅳ级亚组R短于Ⅱ级亚组,Ⅳ级亚组R短于Ⅲ级亚组(P<0.05);Ⅲ级亚组、Ⅳ级亚组K短于Ⅰ级亚组,Ⅳ级亚组K短于Ⅱ级亚组(P<0.05);Ⅲ级亚组、Ⅳ级亚组MA大于Ⅰ级亚组,Ⅲ级亚组MA大于Ⅱ级亚组(P<0.05);Ⅱ级亚组、Ⅲ级亚组、Ⅳ级亚组AA途径抑制率低于Ⅰ级亚组,Ⅳ级亚组AA途径抑制率低于Ⅱ级亚组、Ⅲ级亚组(P<0.05);Ⅱ级亚组、Ⅲ级亚组、Ⅳ级亚组ADP途径抑制率低于Ⅰ级亚组,Ⅲ级亚组、Ⅳ级亚组ADP途径抑制率低于Ⅱ级亚组(P<0.05);Ⅲ级亚组、Ⅳ级亚组血小板凝集率高于Ⅰ级亚组,Ⅳ级亚组血小板凝集率高于Ⅱ级亚组(P<0.05)。中度亚组、重度亚组R短于轻度亚组,重度亚组R短于轻度亚组(P<0.05);重度亚组K短于轻度亚组、中度亚组,MA大于轻度亚组、中度亚组(P<0.05);中度亚组、重度亚组AA途径抑制率、ADP途径抑制率低于轻度亚组,重度亚组AA途径抑制率、ADP途径抑制率低于中度亚组(P<0.05);中度亚组、重度亚组血小板凝集率高于轻度亚组(P<0.05)。结论CAA、ACI患者TEG-PM参数中的R、K均缩短,α角、MA均增大,AA途径抑制率、ADP途径抑制率均降低,且血小板凝集功能均增强。此外,CAA、ACI患者TEG-PM参数中的R、K、AA途径抑制率、ADP途径抑制率均与血小板凝集功能呈负相关,α角、MA均与血小板凝集功能呈正相关。

关键词：冠状动脉疾病冠状动脉粥样硬化脑梗死血栓弹力图血小板图血小板凝集功能

Comprehensive assessment on dynamic roof instability under fractured rock mass conditions in the excavation disturbed zone

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International Journal of Minerals,Metallurgy and Materials 2009年第1期16卷 12-18页

作者： Xing-ping Lai Fen-hua Ren Yong-ping Wu Mei-feng cai Energy School Xi＇an University of Science ＆ Technology Xi＇an 710054 China School of Civil and Environmental Engineering University of Science and Technology Beijing Beijing 100083 China Key Laboratory of Western Mine ＆ Hazard Prevention China Ministry of Education Xi＇an 710054 China

The damage process of fractured rock mass showed that the fracture in rocks induced roof collapse in Yangchangwan Coal Mine, China. The rock mass was particularly weak and fractured. There occurred 6 large-scale dynamical roof falls in the excavation disturbed zone （EDZ） with the collapsing volume of 216 m^3. First, the field detailed geological environment, regional seismic dynamics, and dynamic instability of roadways were generally investigated. Second, the field multiple-index monitoring measurements for detecting the deep delamination of the roof, convergence deformation, bolt-cable load, acoustic emission （AE） characteristic parameters, total AE events, AE energy-releasing rate, rock mass fracture, and damage were arranged. Finally, according to the time-space-strength relations, a quantitative assessment of the influence of rock-mass damage on the dynamic roof instability was accomplished.

关键词： fractured rock mass excavation disturbed zone (EDZ) roof collapse acoustic emission (AE) quantitative assessment

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畅声饮治疗慢性喉炎49例

上海中医药杂志 2002年第12期36卷 18-19页

作者：曹奕黄平蔡建峰马兆鑫陈旭辉上海中医药大学附属岳阳中西医结合医院耳鼻咽喉科上海200437

为观察畅声饮对慢性喉炎的临床疗效,将患者随机分成两组,治疗组(A组)49例,口服畅声饮(由党参、黄芪、熟地黄、苍术等组成)配合雾化吸入庆大霉素加地塞米松,对照组(B组)40例单用雾化吸入治疗。10月为1个疗程,连用3个疗程。结果:A组总有... 详细信息

为观察畅声饮对慢性喉炎的临床疗效,将患者随机分成两组,治疗组(A组)49例,口服畅声饮(由党参、黄芪、熟地黄、苍术等组成)配合雾化吸入庆大霉素加地塞米松,对照组(B组)40例单用雾化吸入治疗。10月为1个疗程,连用3个疗程。结果:A组总有效率为85.7％,B组总有效率为62.5％,两组比较差异有显著性意义(P<0.05)。

关键词：慢性喉炎畅声饮雾化吸入中药疗法