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杂多酸催化合成N,N-双羟乙基硬脂酸氨基乙酯

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江苏化工 1999年第5期27卷 16-17页

作者：蔡照胜盐城轻工业学校盐城224001

以硬脂酸和三乙醇胺为原料在磷钨酸作催化剂、苯作带水剂条件下合成了Ｎ，Ｎ－双羟乙基硬脂酸氨基乙酯，收率在９５％以上。

关键词：羟乙基硬脂酸氨基乙酯表面活性剂杂多酸

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被动控制技术在大跨空间结构中的应用概况

世界地震工程 2009年第3期25卷 25-33页

作者：薛素铎蔡炎城李雄彦曹资北京工业大学建筑工程学院北京100124

结构被动控制技术将传统的依靠结构自身强度和刚度来增强结构抗力的设计方法转变为利用控制装置吸收并耗散振动能量,是抗震防灾的一种积极有效的设计手段。基于被动控制机理,分别从隔震、消能减震和调谐减震3种途径系统总结了被动控制... 详细信息

结构被动控制技术将传统的依靠结构自身强度和刚度来增强结构抗力的设计方法转变为利用控制装置吸收并耗散振动能量,是抗震防灾的一种积极有效的设计手段。基于被动控制机理,分别从隔震、消能减震和调谐减震3种途径系统总结了被动控制技术在大跨空间结构中的应用概况,介绍了相应的控制措施和控制效果,给出了实用性的结论,结果可为大跨空间结构的工程设计提供参考。本成果对推动振动控制手段在大跨空间结构中的实际应用具有积极意义。

关键词：大跨空间结构被动控制隔震消能减震调谐减震

Physiological and anatomical changes in two rapeseed (Brassica napus L.) genotypes under drought stress conditions

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Oil Crop Science 2021年第2期6卷 97-104页

作者： Jiacheng Zhu Dongfang cai Jianping Wang Jinhua Cao yancheng Wen Junping He Lei Zhao Dongguo Wang Shufen Zhang Industrial Crops Research Institute Henan Academy of Agricultural SciencesKey Laboratory of Oil Crops in Huanghuaihai PlainsMinistry of AgricultureKey Laboratory of Oil Crop Genetic Improvement in Henan ProvinceZhengzhouHenan450002China

Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)*** present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes,P287(drought-tolerant)and T88(drought-sensitive)under three intensities of drought *** physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress *** the fourth-leaf stage,the relative water content,chlorophyll content,protein content,malondialdehyde content,and the activities of peroxidase and catalase in P287 were significantly higher than those in T88,particularly under severe drought *** rehydration,all physiological indexes recovered rapidly,especially in *** addition,under drought stress,compared with T88,P287 had thicker palisade tissue,thinner spongy tissue,higher ratio of chloroplast length to chloroplast width,higher stomatal density and stomatal closure ***,the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.

关键词： Brassica napus L. Drought stress Water recovery Physiological parameters

Preliminary investigation and detection based on loop-mediated isothermal amplification(LAMP)of phytoplasmas associated with diseases in *** L.

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Oil Crop Science 2022年第4期7卷 219-224页

作者： yancheng Wen Shufen Zhang Junping He Dongfang cai Jiacheng Zhu Jianping Wang Jinhua Cao Kun Hu Lei Zhao Dongguo Wang Yizi Liu Institute of Industrial Crops of Henan Academy of Agricultural Sciences Zhengzhou450002China

In the last decade,some disease occurred on our experimental farms that had caused serious *** were not caused by fungi,bacteria or *** loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as *** investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in *** L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat *** incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in *** phytoplasma infected plants died without any growing *** studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in *** L.

关键词： B.napus L. Phytoplasma associated disease Pathogen Detection Loop-mediated isothermal amplification (LAMP)

Horizontal flow in a vertically vibrated granular layer

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Particuology 2019年第5期17卷 93-98页

作者： Hui cai Guoqing Miao School of Electrical Engineering Yancheng Institute of Technology Yancheng 224051 China Institute of Acoustics and Key Laboratory of Modem Acoustics of Ministry of Education Nanjing University. Nanjing 210093 China

Motivated by the investigation of Brownian motors in excited granular materials that convert chaotic motion of granules into oriented motion of motors, we performed experimental studies to explore the horizontal flow in a vertically vibrated granular layer in an asymmetric periodic potential. The horizontal flow exhibits complex behaviors, considered as functions of time, space, and driving parameters, which are essentially caused by a thermal ratchet. We interpret our experimental findings in terms of the granular layer gaining, transferring and losing horizontal momentum through inelastic collisions.

关键词： Granular flow Granular materials Thermal ratchets

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mel基因在苏云金芽孢杆菌中的转移和表达

微生物学通报 2004年第2期31卷 72-75页

作者：蔡信之周秋华刘忠权盐城师范学院生物系盐城224002 盐城师范学院化学系盐城224002

苏云金芽孢杆菌 (Bacillusthuringiensis)是研究较深入、使用较广泛的杀虫细菌 ,野外使用易受阳光中紫外线的影响而失活。黑色素具有很强的抗辐射作用。将构建的含有嗜麦芽假单胞菌mel基因的质粒pWSY通过原生质体转化导入苏云金芽孢杆... 详细信息

苏云金芽孢杆菌 (Bacillusthuringiensis)是研究较深入、使用较广泛的杀虫细菌 ,野外使用易受阳光中紫外线的影响而失活。黑色素具有很强的抗辐射作用。将构建的含有嗜麦芽假单胞菌mel基因的质粒pWSY通过原生质体转化导入苏云金芽孢杆菌体内 ,使后者获得了稳定产生黑色素的能力。并在转化子细胞内检测到与供体菌相同的质粒。SDS PAGE显示该转化子体内额外表达了一分子量约为 1 8kD的蛋白 ,该蛋白很可能就是转化子表达的酪氨酸酶。经测定 ,转化子抗辐射作用明显增强。

关键词：嗜麦芽假单胞菌苏云金芽孢杆菌 mel基因转移表达

Optimum Application Time and Application Rate of Nitrogen Fertilizer in Brassica napus L.

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Asian Agricultural Research 2018年第5期10卷 57-61页

作者： Junping HE Shufen ZHANG Jiacheng ZHU Jinhua CAO yancheng WEN Dongfang cai Lei ZHAO Dongguo WANG Jianping WANG Institute of Industrial Crops Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai PlainMinistry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement

To find proper nitrogen fertilizer application mode of rapeseed( Brassica napus L.),promote the high and stable yield of rapeseed,field experiment was carried out to study the effect of nitrogen fertilizer application modes on the yield and economic traits of Fengyou 10 rapeseed planted in two different fertility soils,and characteristics and differences of responses of different fertility soils to nitrogen fertilizer application modes were compared and analyzed. The results showed that the yield and related economic traits of rapeseed treated by nitrogen fertilizer were higher than the control group; in the nitrogen fertilizer treatment group,when the nitrogen fertilizer was applied as the base fertilizer and wintering fertilizer at the ratio of 7∶ 3,the rapeseed yield was higher than the control group and reached the highest; when the nitrogen fertilizer was applied as the base fertilizer,wintering fertilizer,and bud fertilizer at the ratio of 5∶ 3∶ 2,the rapeseed yield was significantly higher than the control group but was the lowest. These indicate that sufficient nitrogen supply is the basis for normal growth and development of rape seedlings. When the pure nitrogen fertilizer was at 192 kg/ha and the ratio of base fertilizer and wintering fertilizer was 7∶ 3,it can better coordinate the high yield of rapeseed and overall use of nitrogen fertilizer,and obtain higher rapeseed yield.

关键词： Brassica napus L. Nitrogen fertilizer application Yield Economic traits Soil fertility

秦皮苷减轻LPS诱导的肺炎小鼠肺组织炎症和氧化损伤

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免疫学杂志 2021年第9期37卷 809-815页

作者：徐宜全蔡忠贵潘延成滕州市中医医院呼吸内科 277599 枣庄矿业集团枣庄医院内科 277100 滕州市中心人民医院药房 277599

目的研究秦皮苷(fraxin)对脂多糖(lipopolysaccharides,LPS)诱导的肺炎小鼠肺组织炎症和氧化损伤的影响。方法实验分成Control(空白对照)、Model(肺炎模型)、fraxin-A(肺炎模型,8 mg/kg秦皮甙灌胃)、fraxin-B(肺炎模型,16 mg/kg秦皮甙... 详细信息

目的研究秦皮苷(fraxin)对脂多糖(lipopolysaccharides,LPS)诱导的肺炎小鼠肺组织炎症和氧化损伤的影响。方法实验分成Control(空白对照)、Model(肺炎模型)、fraxin-A(肺炎模型,8 mg/kg秦皮甙灌胃)、fraxin-B(肺炎模型,16 mg/kg秦皮甙灌胃治疗)、fraxin-C组(肺炎模型,32 mg/kg秦皮甙灌胃)。取肺组织和肺泡灌洗液,检测肺组织湿/干重比例,计数肺泡灌洗液细胞总数和中性粒细胞数目;ELISA法检测肺泡灌洗液中白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-10(interleukin-10,IL-10)含量;比色法检测肺泡灌洗液中超氧化物歧化酶(superoxide orgotein dismutase,SOD)和一氧化氮(nitric oxide,NO)含量;可见分光光度法检测肺泡灌洗液中丙二醛(malonaldehyde,MDA)含量;Western blot法检测肺组织中p-NF-κBp65、核转录因子-κB(nuclear factor-kappaB,NF-κB)p65、Toll样受体4(Toll-like receptor-4,TLR4)、诱导型一氧化氮合成酶(inducible nitric oxide synthase,iNOS)、p-IκBα、IκBα蛋白表达。结果与Control组比较,Model组肺组织湿/干重比例升高,肺泡灌洗液细胞总数和中性粒细胞数目增多,肺泡灌洗液中IL-6、IL-1β、TNF-α含量和MDA、NO含量升高,SOD含量下降,肺组织中NF-κBp65、TLR4、i NOS蛋白水平升高。与Model组比较,fraxin-A、fraxin-B、fraxin-C组肺组织湿/干重比例降低,肺泡灌洗液细胞总数和中性粒细胞数目减少,肺泡灌洗液中IL-6、IL-1β、TNF-α含量和MDA、NO含量降低,SOD含量升高,肺组织中NF-κBp65、TLR4、iNOS蛋白水平降低。结论秦皮苷减轻LPS诱导的肺炎小鼠肺组织炎症和氧化损伤,其机制可能与TLR4/NF-κB有关。

关键词：秦皮苷肺炎炎症氧化损伤

Coupling simulation of fluid structure interaction in the stirred vessel with a pitched blade turbine

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Chinese Journal of Chemical Engineering 2018年第5期26卷 922-929页

作者： Yangyang Liang Zhengming Gao Dai'en Shi Wanli Zhao Ziqi cai State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Beijing 100029 China Mechanical Engineering School Yancheng Institute of Technology Yancheng 224051 China

The interaction between fluid and a down-pumping pitched blade turbine fixed with a flexible shaft in the stirred vessel, as a typical fluid structure interaction phenomenon, was simulated by coupling the Computational Fluid Dynamics and Computational Structural Dynamics. Based on the verification of the simulated impeller torque and dimensionless shaft bending moment with experimental result, the dimensionless shaft bending moment and various loads acting on impeller(including lateral force, axial force and bending moment) were discussed in detail. By separating and extracting the fluid and structural components from those loads, the results show that the shaft bending moment mainly results from the lateral force on impeller although the axial force on impeller is much larger. The impeller mass imbalance increases the shaft bending moment and the lateral force on impeller, but has little influence on the axial force and bending moment acting on impeller. The dominant frequencies of impeller forces are macro-frequency, speed frequency and blade passing frequency, and are associated with the impeller mass imbalance.

关键词： Fluid structure interaction Shaft bending moment Impeller lateral force Impeller axial force Bending moment on impeller